ISOLATION OF LABELLED DEOXYRIBONUCLEIC ACID PHOSPHORUS BY A MODIFIED SCHMIDT AND THANNHAUSER PROCEDURE

1957 ◽  
Vol 35 (9) ◽  
pp. 721-728 ◽  
Author(s):  
R. Daoust ◽  
Catherine E. Stevens Hooper
Keyword(s):  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Specific Activity ◽  
Phosphorus Fraction ◽  
Modified Method ◽  
Phosphorus Containing ◽  
Soluble Phosphorus

The DNA phosphorus fraction of tissues was isolated by the method of Schmidt and Thannhauser modified so that the DNA phosphorus fraction attains a state of purity satisfactory for studies with radiophosphorus. This procedure was submitted to a series of test experiments. The results suggested that the DNA isolated by the procedure is practically free from contamination by other phosphorus-containing fractions, namely acid-soluble phosphorus, phospholipids, ribonucleic acid, and phosphoproteins. The modified method seems reliable for estimating the specific activity of DNA phosphorus following the administration of radiophosphorus to animals.

ISOLATION OF LABELLED DEOXYRIBONUCLEIC ACID PHOSPHORUS BY A MODIFIED SCHMIDT AND THANNHAUSER PROCEDURE

1957 ◽  
Vol 35 (1) ◽  
pp. 721-728 ◽  
Author(s):  
R. Daoust ◽  
Catherine E. Stevens Hooper
Keyword(s):  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Specific Activity ◽  
Phosphorus Fraction ◽  
Modified Method ◽  
Phosphorus Containing ◽  
Soluble Phosphorus

The DNA phosphorus fraction of tissues was isolated by the method of Schmidt and Thannhauser modified so that the DNA phosphorus fraction attains a state of purity satisfactory for studies with radiophosphorus. This procedure was submitted to a series of test experiments. The results suggested that the DNA isolated by the procedure is practically free from contamination by other phosphorus-containing fractions, namely acid-soluble phosphorus, phospholipids, ribonucleic acid, and phosphoproteins. The modified method seems reliable for estimating the specific activity of DNA phosphorus following the administration of radiophosphorus to animals.

The Phosphorus-containing Fractions of Sterile Lingcod Muscle During Storage at 0 °C

1960 ◽  
Vol 17 (3) ◽  
pp. 371-376 ◽  
Author(s):  
Neil Tomlinson ◽  
Vera M. Creelman ◽  
K. G. Reid
Keyword(s):  
Aqueous Extract ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Soluble Fraction ◽  
Inorganic P ◽  
P Content ◽  
Phosphorylated Compounds ◽  
Phosphorus Containing ◽  
Total P

During refrigerator storage of sterile lingcod muscle for up to 3 weeks at 0 °C no significant changes occurred in the P content of the phospholipid, ribonucleic acid, or deoxyribonucleic acid fractions, but in the acid-soluble fraction the portion of total P accounted for by inorganic P increased to 96% from 75%. The P-containing fractions from the fish held in ice for the same period did not differ appreciably from those of the sterile muscle. An aqueous extract of lingcod muscle was shown to be able to form orthophosphate from a variety of phosphorylated compounds.

scholarly journals The Enzymatic Phosphorylation of Ribonucleic Acid and Deoxyribonucleic Acid

1966 ◽  
Vol 241 (12) ◽  
pp. 2933-2943 ◽  
Author(s):  
Abraham Novogrodsky ◽  
Moshe Tal ◽  
Abraham Traub ◽  
Jerard Hurwitz
Keyword(s):  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Enzymatic Phosphorylation
Sign in / Sign up

Export Citation Format

Share Document