Multiple variants and a differential splicing pattern of kinectin in human hepatocellular carcinoma

2004 ◽  
Vol 82 (2) ◽  
pp. 321-327 ◽  
Author(s):  
Hong-Cheng Wang ◽  
Yan-Rong Su ◽  
Ke-Jun Han ◽  
Xue-Wen Pang ◽  
Ji-Run Peng ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Alternative Splicing ◽  
Tissue Sample ◽  
Cdna Expression Library ◽  
Serological Analysis ◽  
Expression Library ◽  
Humoral Immune ◽  
Cdna Expression ◽  
Splicing Pattern ◽  
Alternatively Spliced

To extend the search for hepatocellular carcinoma (HCC) associated antigens with immunogenicity for clinical applications, we constructed a cDNA expression library using resected human HCC tissue sample and screened it by serological analysis of recombinant cDNA expression library (SEREX) with autologous and allogeneic sera. A total of 24 distinct antigens were isolated and kinectin was the antigen most frequently identified. We found that kinectin was alternatively spliced at four sites and obtained all eight theoretical forms of variant, six by SEREX and two by RT-PCR, from the different splicing combinations of the last three sites. In addition, the splicing patterns of four sites were analyzed. Variant containing D2 was overexpressed in cancerous tissues and this alteration may be tumor associated. The four splicing sites, the variants generated by alternative splicing, and the humoral immune response in HCC patients, may help to analyze the role of kinectin in human HCC cell biology.Key words: alternative splicing, antibody response, hepatocellular carcinoma, kinectin, serological analysis of recombinant cDNA expression library (SEREX).

Identification of novel IgE-binding soybean allergens using serological analysis of a recombinant cDNA expression library (SEREX)

2014 ◽  
Vol 23 (4) ◽  
pp. 1037-1042 ◽  
Author(s):  
Jae-Hwan Kim ◽  
Kang-Mo Ahn ◽  
Wooki Kim ◽  
Youngshin Han ◽  
Young-Rok Kim ◽  
...  
Keyword(s):  
Cdna Expression Library ◽  
Serological Analysis ◽  
Expression Library ◽  
Ige Binding ◽  
Cdna Expression

scholarly journals Trans-acting Factors Required for Inclusion of Regulated Exons in the Ultrabithorax mRNAs of Drosophila melanogaster

Genetics ◽  
1999 ◽  
Vol 151 (4) ◽  
pp. 1517-1529 ◽  
Author(s):  
James M Burnette ◽  
Allyson R Hatton ◽  
A Javier Lopez
Keyword(s):  
Drosophila Melanogaster ◽  
Alternative Splicing ◽  
P Element ◽  
Splicing Factors ◽  
Loss Of Function ◽  
Large Collection ◽  
Splicing Pattern ◽  
Alternatively Spliced ◽  
Hypomorphic Alleles

Abstract Alternatively spliced Ultrabithorax mRNAs differ by the presence of internal exons mI and mII. Two approaches were used to identify trans-acting factors required for inclusion of these cassette exons. First, mutations in a set of genes implicated in the control of other alternative splicing decisions were tested for dominant effects on the Ubx alternative splicing pattern. To identify additional genes involved in regulation of Ubx splicing, a large collection of deficiencies was tested first for dominant enhancement of the haploinsufficient Ubx haltere phenotype and second for effects on the splicing pattern. Inclusion of the cassette exons in Ubx mRNAs was reduced strongly in heterozygotes for hypomorphic alleles of hrp48, which encodes a member of the hnRNP A/B family and is implicated in control of P-element splicing. Significant reductions of mI and mII inclusion were also observed in heterozygotes for loss-of-function alleles of virilizer, fl(2)d, and crooked neck. The products of virilizer and fl(2)d are also required for Sxl autoregulation at the level of splicing; crooked neck encodes a protein with structural similarities to yeast-splicing factors Prp39p and Prp42p. Deletion of at least five other loci caused significant reductions in the inclusion of mI and/or mII. Possible roles of identified factors are discussed in the context of the resplicing strategy for generation of alternative Ubx mRNAs.

scholarly journals Study on Characteristics of Chemokine CXCL10 Gene Cloned from cDNA Expression Library of Ujumqin Sheep

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
P. F. Hu ◽  
X. C. Li ◽  
N. Lei ◽  
X. Y. Lan ◽  
Q. J. Zhao ◽  
...  
Keyword(s):  
Immune Responses ◽  
Transfection Efficiency ◽  
Peak Level ◽  
Fluorescence Signal ◽  
Cdna Expression Library ◽  
Fibroblast Cells ◽  
Green Fluorescence ◽  
Cdna Fragment ◽  
Expression Library ◽  
Cdna Expression

Chemokines were a major regulator of body’s inflammatory and immune responses. In this study, the cDNA fragment of chemokine CXC ligand 10 (CXCL10) was cloned from the Ujumqin sheep ear marginal tissue cDNA expression library; the CXCL10 gene had 103 amino acids and a molecular weight of 11.47 kDa, and it shared a high homology among cattle, sheep, and goat, while a low homology compared with mouse. The CXCL10 protein had 4 conservative cysteine residues, located in 28, 30, 55, and 72 sites. The expression pattern and intracellular distribution of recombinant CXCL10 proteins in Ujumqin sheep fibroblast cells showed that there were green fluorescence signals both in cytoplasm and nucleolus after 24 h of transfection, the number of positive cells was increased with time, the peak level of fluorescence signal was reached after 48 h of transfection and the transfection efficiency was 33.3%; there was a significant decrease in fluorescence intensity after 72 h of transfection. Expression of recombinant CXCL10 gene inEscherichia colihad a time- and temperature-dependency on the amount of protein expression, and a small quantity of inducer was needed.

scholarly journals Bovine galactosyltransferase: identification of a clone by direct immunological screening of a cDNA expression library.

1986 ◽  
Vol 83 (6) ◽  
pp. 1573-1577 ◽  
Author(s):  
N. L. Shaper ◽  
J. H. Shaper ◽  
J. L. Meuth ◽  
J. L. Fox ◽  
H. Chang ◽  
...  
Keyword(s):  
Cdna Expression Library ◽  
Expression Library ◽  
Cdna Expression ◽  
Immunological Screening
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