Helicobacter pylorifrom asymptomatic hosts expressing heptoglycan but lacking Lewis O-chains: Lewis blood-group O-chains may play a role inHelicobacter pyloriinduced pathology

2001 ◽  
Vol 79 (4) ◽  
pp. 449-459 ◽  
Author(s):  
Mario A Monteiro ◽  
Frank St Michael ◽  
David A Rasko ◽  
Diane E Taylor ◽  
J Wayne Conlan ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Blood Group ◽  
Lipid A ◽  
Blood Groups ◽  
Blood Group Antigens ◽  
Chemical Structures ◽  
Lewis Blood Group ◽  
Membrane Antigens ◽  
H Pylori ◽  
Group Structures

Helicobacter pylori is a widespread Gram-negative bacterium responsible for the onset of various gastric pathologies and cancers in humans. A familiar trait of H. pylori is the production of cell-surface lipopolysaccharides (LPSs; O-chain [Formula: see text] core [Formula: see text] lipid A) with O-chain structures analogous to some mammalian histo-blood-group antigens, those being the Lewis determinants (Lea, Leb, Lex, sialyl Lex, Ley) and blood groups A and linear B. Some of these LPS antigens have been implicated as autoimmune, adhesion, and colonization components of H. pylori pathogenic mechanisms. This article describes the chemical structures of LPSs from H. pylori isolated from subjects with no overt signs of disease. Experimental data from chemical- and spectroscopic-based studies unanimously showed that these H. pylori manufactured extended heptoglycans composed of 2- and 3-linked D-glycero-α-D-manno-heptopyranose units and did not express any blood-group O-antigen chains. The fact that another H. pylori isolate with a similar LPS structure was shown to be capable of colonizing mice indicates that H. pylori histo-blood-group structures are not an absolute prerequisite for colonization in the murine model also. The absence of O-chains with histo-blood groups may cause H. pylori to become inept in exciting an immune response. Additionally, the presence of elongated heptoglycans may impede exposure of disease-causing outer-membrane antigens. These factors may render such H. pylori incapable of creating exogenous contacts essential for pathogenesis of severe gastroduodenal diseases and suggest that histo-blood groups in the LPS may indeed play a role in inducing a more severe H. pylori pathology.Key words: lipopolysaccharide, carbohydrates, glycobiology, Helicobacter pylori, histo-blood groups.

scholarly journals Associations between seroprevalence of Helicobacter pylori and ABO/rhesus blood group antigens in healthy blood donors in southwest Iran

2021 ◽  
Vol 49 (12) ◽  
pp. 030006052110588
Author(s):  
Azar Dokht Khosravi ◽  
Mehrandokht Sirous ◽  
Morteza Saki ◽  
Sakineh Seyed-Mohammadi ◽  
Seyed Reza Modares Mousavi ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Blood Group ◽  
Blood Donors ◽  
Blood Groups ◽  
Blood Type ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Group Antigens ◽  
Chi Square ◽  
Cytotoxin Associated Gene A ◽  
H Pylori

Objective To investigate correlations between ABO/rhesus (Rh) blood group antigens and anti- Helicobacter pylori and anti-cytotoxin-associated gene A (CagA) seropositivity in blood donors. Methods A total of 311 blood donors were enrolled. ABO and Rh blood groups were determined using hemagglutination tests. Specific anti- H. pylori IgG and anti-CagA IgG antibodies in sera were quantitated by enzyme-linked immunosorbent assay. Correlations between blood groups and anti- H. pylori and anti-CagA seropositivity were evaluated using the Chi-square test. Results O+ was the most frequent blood type (38%, n = 118). Anti- H. pylori IgG seropositivity was observed in 240 (77.2%) blood donors, while anti-CagA IgG seropositivity was observed in 132 (42.5%) blood donors. Although seropositivity rates for both anti- H. pylori and anti-CagA IgG were higher in individuals with blood type O, no statistically significant associations were observed between seropositivity and any ABO/Rh blood groups. Conclusion Individuals with blood type O may have higher rates of H. pylori seropositivity.

scholarly journals Potential role of molecular mimicry between Helicobacter pylori lipopolysaccharide and host Lewis blood group antigens in autoimmunity.

1996 ◽  
Vol 64 (6) ◽  
pp. 2031-2040 ◽  
Author(s):  
B J Appelmelk ◽  
I Simoons-Smit ◽  
R Negrini ◽  
A P Moran ◽  
G O Aspinall ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Blood Group ◽  
Potential Role ◽  
Molecular Mimicry ◽  
Blood Group Antigens ◽  
Lewis Blood Group

Characterization of murine monoclonal antibodies againstHelicobacter pylorilipopolysaccharide specific for Lexand Leyblood group determinants

2005 ◽  
Vol 83 (5) ◽  
pp. 589-596 ◽  
Author(s):  
Eleonora Altman ◽  
Blair A Harrison ◽  
Tomoko Hirama ◽  
Vandana Chandan ◽  
Rebecca To ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Monoclonal Antibodies ◽  
Blood Group ◽  
Cell Envelope ◽  
Mucosal Cell ◽  
Blood Group Antigens ◽  
Binding Characteristics ◽  
Igg3 Subclass ◽  
H Pylori

The cell envelope of Helicobacter pylori contains lipopolysaccharide (LPS), the O-chain of which expresses type 2 Lexand Leyblood group antigens, which mimic human gastric mucosal cell-surface glycoconjugates and may contribute to the survival of H. pylori in gastric mucosa. Here we describe the generation of monoclonal antibodies specific for Lexand Leyblood group determinants and the characterization of their binding properties using purified, structurally defined H. pylori LPS, synthetic glycoconjugates, and H. pylori cells. Analysis of oligosaccharide binding by SPR provided a rapid and reliable means for characterization of antibody affinities. One of the antibodies, anti-Lex, was of IgG3 subclass and had superior binding characteristics as compared with the commercially available anti-LexIgM. These antibodies could have potential in the immunodiagnosis of certain types of cancer, in serotyping of H. pylori isolates, and in structure–function studies.Key words: Helicobacter pylori, lipopolysaccharide, monoclonal antibodies, Lewis determinants, immunodiagnosis.

scholarly journals Relationship of Blood Group Determinants on Helicobacter pylori Lipopolysaccharide with Host Lewis Phenotype and Inflammatory Response

2000 ◽  
Vol 68 (2) ◽  
pp. 937-941 ◽  
Author(s):  
Michael A. Heneghan ◽  
Ciaran F. McCarthy ◽  
Anthony P. Moran
Keyword(s):  
Helicobacter Pylori ◽  
Inflammatory Response ◽  
Blood Group ◽  
Chronic Gastritis ◽  
Bacterial Colonization ◽  
Neutrophil Infiltration ◽  
Blood Group Antigens ◽  
Significant Difference ◽  
H Pylori ◽  
Relationship Of

ABSTRACT As Lewis a (Lea) and Lewis b (Leb) blood group antigens are isoforms of Lewis x (Lex) and Lewis y (Ley) and are expressed in the gastric mucosa, we evaluated whether the patterns of expression of Lex and Ley on Helicobacter pylorilipopolysaccharides reflected those of host expression of Lea and Leb. When 79 patients (secretors and nonsecretors) were examined for concordance between bacterial and host Le expression, no association was found (χ2 = 5.734, 3 df, P = 0.125), nor was there a significant difference between the amount of Lex or Ley expressed on isolates from ulcer and chronic gastritis patients (P > 0.05). Also, the effect of host and bacterial expression of Le antigens on bacterial colonization and the observed inflammatory response was assessed. In ulcer patients, Lex expression was significantly related to neutrophil infiltration (r s = 0.481,P = 0.024), whereas in chronic gastritis patients significant relationships were found between Lexexpression and H. pylori colonization density (r s = 0.296, P = 0.03), neutrophil infiltrate (r s = 0.409,P = 0.001), and lymphocyte infiltrate (r s = 0.389, P = 0.002). Furthermore, bacterial Ley expression was related to neutrophil (r s = 0.271, P= 0.033) and lymphocyte (r s = 0.277,P = 0.029) infiltrates. Thus, although no evidence of concordance was found between bacterial and host expression of Le determinants, these antigens may be crucial for bacterial colonization, and the ensuing inflammatory response appears, at least in part, to be influenced by Le antigens.

scholarly journals Helicobacter pylori infection according to ABO blood group among blood donors in Kosovo

2011 ◽  
Vol 1 (2) ◽  
pp. 83-89 ◽  
Author(s):  
Bukurije Zhubi ◽  
Zana Baruti-Gafurri ◽  
Ymer Mekaj ◽  
Mimoza Zhubi ◽  
Idriz Merovci ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Blood Group ◽  
Blood Donors ◽  
Mean Value ◽  
Blood Groups ◽  
Helicobacter Pylori Infection ◽  
Igg Antibody ◽  
Significant Difference ◽  
Rhesus Blood Group ◽  
H Pylori

Introduction: Numerous studies have reported a high prevalence of Helicobacter pylori infection among healthy and non-healthy persons in different places. The Aim of the study is to investigate the seroprevalence of H. pylori infection among Kosovo’s Blood donor associated with ABO/Rhesus blood group.Methods: 671 blood donors are tested for H. pylori antibodies and results are classifi ed by way of donation, age, gender, blood groups and education level. Serum antibodies are analyzed by Enzyme Linked Fluorescent Assay test for H. pylori IgG with Biomerieux HPY-VIDAS.Results: The frequency of IgG antibody for H. pylori among healthy blood donors is 56.9%, there is not found any difference between voluntary and non-voluntary blood donors (57.4% respectively 56.3%)(OR=1.05; 95% CI 0.76 to 1.43; p=0.8). H pylori IgG antibodies positive are detected in 57.0 % ( 126 of 221) of women, compared with 56.9 % ( 256 of 450) of men(OR=0.99; 95% CI 0.72 to 1.38; p=0.96). Serpositive donors are older than seronegative ones (31.9 years, respectively 29.5 years, p=0.02). Mean value of IgG antibody of H. pylori is 3.61 with no significant difference between males and females (3.72 respectively 3.44; p=0.2). The seroprevalence of H. pylori infection is similar among blood groups: O (57.4%), A (56.2%), B (59.6%), AB (51.4%), RhD positive (56.7%) and RhD negative (58.3%).Conclusions: The seropositivity of H. pylori is moderately higher in the non voluntary and familiar blood donors among the total Kosovo blood donors. There is not found a significant relationship between infection with H. pylori and ABO/Rhesus blood group among blood donors.

scholarly journals Helicobacter pylori infection Evluated by C14-urea Breath test and it`s Relation with age, sex and ABO/Rhesus blood Groups in Patients with Gastrointestinal complaints in Kirkuk City / Iraq.

2019 ◽  
Vol 24 (4) ◽  
pp. 12
Author(s):  
Nadia. M. Mohammad1 ◽  
Sabah M. Salih2
Keyword(s):  
Helicobacter Pylori ◽  
Blood Group ◽  
Breath Test ◽  
Blood Groups ◽  
Urea Breath Test ◽  
Helicobacter Pylori Infection ◽  
Positive Test ◽  
Significant Difference ◽  
Gastrointestinal Complaints ◽  
H Pylori

There was significant difference between genders and H. pylori  infection (P<0.05). From the 176 patients attending private laboratories in Kirkuk city for the period from 1/8/2016 to 1/4/2017, 86(48.86%) belonged to blood group O, 42(23.86%) to A, 41(23.29%) to B and 7 (3.97%) to AB and There was statistically significant difference (P<0.05) in the incidence of H. pylori  infection between these groups, there was significant correlation between the presence of  particular blood group in H. pylori positive patients related to the reported frequency of the blood groups in Kirkuk population, the correlation between the Rh factor and positive H. pylori  patients was not significant to the frequency of the Rh factor in the population (88.06% Rh+ and 11.93% Rh-). the H. pylori  positive test was slightly, but not significantly lower in comparison with the negitive H. pylori patients test values showed a highly significant difference (P<0.01) in H. pylori posative and H. pylori negitive patients., in adults H. pylori  infection depend upon gender, blood groups but they do not depend upon the Patients age or Rh factore.   http://dx.doi.org/10.25130/tjps.24.2019.064

scholarly journals ABO, Lewis, secretor and non-secretor phenotypes in patients infected or uninfected by the Helicobacter pylori bacillus

2002 ◽  
Vol 120 (2) ◽  
pp. 55-58 ◽  
Author(s):  
Luiz Carlos de Mattos ◽  
Juliana Rodrigues Cintra ◽  
Fábio Eduardo Sanches ◽  
Rita de Cássia Martins Alves da Silva ◽  
Milton Artur Ruiz ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Blood Group ◽  
Epidemiological Studies ◽  
Blood Type ◽  
Homogeneous Distribution ◽  
Peptic Ulcers ◽  
Cross Sectional ◽  
Lewis Blood Group ◽  
Hemagglutination Test ◽  
H Pylori

CONTEXT: Epidemiological studies have demonstrated higher frequencies of the O blood group and the non-secretor phenotype of ABH antigens among patients suffering from peptic ulcers. Since Helicobacter pylori has been established as the main etiological factor in this disease, controversies about the associations of the ABO and Lewis blood group phenotypes and secretor and non-secretor phenotypes in relation to susceptibility towards infection by this bacillus have been presented. OBJECTIVE: To verify the frequencies of ABO, Lewis blood group phenotypes, secretor and non-secretor phenotypes in patients infected or uninfected by H. pylori. DESIGN: Cross-sectional study. SETTING: Outpatient clinic. PARTICIPANTS: One hundred and twenty patients with dyspeptic symptoms who underwent endoscopy.MAIN MEASUREMENTS: ABO and Lewis blood group phenotypes were determined by a standard hemagglutination test and the secretor and non-secretor phenotypes were evaluated by saliva samples using the inhibitor hemagglutination test. RESULTS: The diagnosis of infection, made via breath and urea tests and confirmed using polymerase chain reaction (PCR) in gastric biopsy fragments, showed the presence of H. pylori in 61.7% of the patients and absence in 38.3%. The differences between the frequencies of the ABO blood group phenotypes among infected (A 27.0%; B 12.2%; AB 4.0% and O 56.8%) and uninfected patients (A 58.7%; B 13.0%; AB 4.3% and O 24.0%) were significant. The Lewis blood type, secretor and non-secretor phenotypes showed homogeneous distribution between the groups of patients analyzed. CONCLUSIONS: Our results suggest that the infection of H. pylori can be related to ABO blood groups but not to the Lewis blood group nor to secretor and non-secretor phenotypes.

scholarly journals Rhesus monkey gastric mucins: oligomeric structure, glycoforms and Helicobacter pylori binding1

2004 ◽  
Vol 379 (3) ◽  
pp. 765-775 ◽  
Author(s):  
Sara LINDÉN ◽  
Thomas BORÉN ◽  
André DUBOIS ◽  
Ingemar CARLSTEDT
Keyword(s):  
Helicobacter Pylori ◽  
Rhesus Monkey ◽  
Blood Group ◽  
T Antigen ◽  
Blood Group Antigens ◽  
Human Beings ◽  
Sialyl Lex ◽  
H Pylori

Mucins isolated from the stomach of Rhesus monkey are oligomeric glycoproteins with a similar mass, density, glycoform profile and tissue localization as human MUC5AC and MUC6. Antibodies raised against the human mucins recognize those from monkey, which thus appear to be orthologous to those from human beings. Rhesus monkey muc5ac and muc6 are produced by the gastric-surface epithelium and glands respectively, and occur as three distinct glycoforms. The mucins are substituted with the histo blood-group antigens B, Lea (Lewis a), Leb, Lex, Ley, H-type-2, the Tn-antigen, the T-antigen, the sialyl-Lex and sialyl-Lea structures, and the expression of these determinants varies between individuals. At neutral pH, Helicobacter pylori strains expressing BabA (blood-group antigen-binding adhesin) bind Rhesus monkey gastric mucins via the Leb or H-type-1 structures, apparently on muc5ac, as well as on a smaller putative mucin, and binding is inhibited by Leb or H-type-1 conjugates. A SabA (sialic acid-binding adhesin)-positive H. pylori mutant binds to sialyl-Lex-positive mucins to a smaller extent compared with the BabA-positive strains. At acidic pH, the microbe binds to mucins substituted by sialylated structures such as sialyl-Lex and sialylated type-2 core, and this binding is inhibited by DNA and dextran sulphate. Thus mucin–H. pylori binding occurs via at least three different mechanisms: (1) BabA-dependent binding to Leb and related structures, (2) SabA-dependent binding to sialyl-Lex and (3) binding through a charge-mediated mechanism to sialylated structures at low pH values.

Association of Recurrent Candidal Vaginitis with Inheritance of Lewis Blood Group Antigens

1995 ◽  
Vol 172 (6) ◽  
pp. 1616-1619 ◽  
Author(s):  
E. Hilton ◽  
V. Chandrasekaran ◽  
P. Rindos ◽  
H. D. Isenberg
Keyword(s):  
Blood Group ◽  
Blood Group Antigens ◽  
Lewis Blood Group
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