Nodulation ofPhaseolus vulgarisbyRhizobium etliis enhanced by the presence ofBacillus

1997 ◽  
Vol 43 (1) ◽  
pp. 1-8 ◽  
Author(s):  
M. Srinivasan ◽  
F. B. Holl ◽  
D. J. Petersen
Keyword(s):  
Phaseolus Vulgaris ◽  
Lateral Root ◽  
Root Formation ◽  
Bacterial Species ◽  
Plant Roots ◽  
Rhizobium Etli ◽  
Phaseolus Acutifolius ◽  
Plant Interaction ◽  
Split Root ◽  
Bacillus Spp

The ability of Bacillus spp. to alter the nodulation of Phaseolus vulgaris by Rhizobium etli was assessed. The simultaneous presence of both Rhizobium etli TAL 182 and Bacillus megaterium S49 on plant roots during the early stages of plant growth was necessary for enhanced nodulation of Phaseolus vulgaris by the Rhizobium microsymbiont. Coinoculation with both bacterial species also facilitated heterologous nodulation of Rhizobium TAL 182 on Phaseolus acutifolius. These results are consistent with earlier reports of increased root hair proliferation and lateral root formation in response to coinoculation. Split-root experiments revealed that coinoculation partially suppressed host-controlled regulation of nodulation, implicating a plant interaction with the two bacterial species. Changes to the nodulation potential of R. etli due to coinoculation with Bacillus spp. demonstrate the potential for root-associated organisms other than rhizobia to alter the dynamics of the legume–Rhizobium symbiosis.Key words: Bacillus, nodulation enhancement, heterologous nodulation.

Assessment of Bacterial Contamination of Orthodontic Arch wire

2019 ◽  
Vol 31 (1) ◽  
pp. 48-51
Author(s):  
Suha S Hassan ◽  
Nidhal H. Ghaib ◽  
Batool H Al-Ghurabi
Keyword(s):  
Bacterial Contamination ◽  
Microbial Contamination ◽  
Ethylenediaminetetraacetic Acid ◽  
Bacterial Species ◽  
Brain Heart Infusion ◽  
Control Groups ◽  
Dental Practitioners ◽  
Significant Difference ◽  
Bacillus Spp ◽  
Droplet Transmission

Background: The microorganisms can impend the life of health care professional and particularly the dental practitioners. They can be transmitted by different ways like airborne and droplet transmission. The current study was carried out to identify whether the arch wires that received from the manufactures are free from microbial contamination and to determine the bacterial species attached to the arch wires. Materials and Methods: This study involved eighty samples, consisted of two types of arch wires (nitinol and stainless-steel) from four companies (3M, G&H, Jiscop, OrthoTechnology). These wires inserted in a plane tube that contains 10 -ml of (Tris [tris(hydroxymethyl)aminomethane] and EDTA (ethylenediaminetetraacetic acid) tris-EDTA and brain heart infusion (BHI) broth. A 0.1 ml was withdrawn from the tube and spread on agar plates. The control groups consist of 16 plane tube (8 tubes with tris-EDTA and other 8 tubes with (BHI). Results: Microbial sampling yielded growth from 5 of the 80 arch wires. The predominant bacteria that isolated were Bacillus spp. No growth was recovered from 75 of the samples and from controls. The bacteria were isolated by BHI reagent and no growth was observed by tris-EDTA reagent with statistically significant difference (P<0.05). The Bacillus spp. found only in the G&H and Jiscop companies, however, no statistically significant difference was found among them (P>0.05). With regard to the presence and distribution of bacteria according to the types of wires, the present results clarified that cases of contamination with Bacillus spp. were found in the nitinol arch wires with statistically significant difference (P<0.05). Conclusions: The results of the current study revealed low count of bacterial contamination in the two types of companies (G&H and Jiscop). Not all materials that received from the manufactures are free from contamination and an effective sterilization regimen is needed to avoid cross-contamination.

Lateral root formation and nutrients: nitrogen in the spotlight

2021 ◽  
Author(s):  
Pierre-Mathieu Pélissier ◽  
Hans Motte ◽  
Tom Beeckman
Keyword(s):  
Signaling Pathways ◽  
Root System ◽  
Root Development ◽  
Lateral Root ◽  
Molecular Mechanisms ◽  
Root Formation ◽  
Lateral Roots ◽  
Nutrient Use Efficiency ◽  
Lateral Root Formation ◽  
Lateral Root Development

Abstract Lateral roots are important to forage for nutrients due to their ability to increase the uptake area of a root system. Hence, it comes as no surprise that lateral root formation is affected by nutrients or nutrient starvation, and as such contributes to the root system plasticity. Understanding the molecular mechanisms regulating root adaptation dynamics towards nutrient availability is useful to optimize plant nutrient use efficiency. There is at present a profound, though still evolving, knowledge on lateral root pathways. Here, we aimed to review the intersection with nutrient signaling pathways to give an update on the regulation of lateral root development by nutrients, with a particular focus on nitrogen. Remarkably, it is for most nutrients not clear how lateral root formation is controlled. Only for nitrogen, one of the most dominant nutrients in the control of lateral root formation, the crosstalk with multiple key signals determining lateral root development is clearly shown. In this update, we first present a general overview of the current knowledge of how nutrients affect lateral root formation, followed by a deeper discussion on how nitrogen signaling pathways act on different lateral root-mediating mechanisms for which multiple recent studies yield insights.

scholarly journals Evaluation of MicroScan Bacterial Identification Panels for Low-Resource Settings

Diagnostics ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 349
Author(s):  
Sien Ombelet ◽  
Alessandra Natale ◽  
Jean-Baptiste Ronat ◽  
Olivier Vandenberg ◽  
Liselotte Hardy ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Ease Of Use ◽  
Bacterial Identification ◽  
Gram Positive ◽  
Gram Negative ◽  
Low Resource Settings ◽  
Low Resource ◽  
Bacillus Spp ◽  
Gram Negative Organisms ◽  
Instructions For Use

Bacterial identification is challenging in low-resource settings (LRS). We evaluated the MicroScan identification panels (Beckman Coulter, Brea, CA, USA) as part of Médecins Sans Frontières’ Mini-lab Project. The MicroScan Dried Overnight Positive ID Type 3 (PID3) panels for Gram-positive organisms and Dried Overnight Negative ID Type 2 (NID2) panels for Gram-negative organisms were assessed with 367 clinical isolates from LRS. Robustness was studied by inoculating Gram-negative species on the Gram-positive panel and vice versa. The ease of use of the panels and readability of the instructions for use (IFU) were evaluated. Of species represented in the MicroScan database, 94.6% (185/195) of Gram-negative and 85.9% (110/128) of Gram-positive isolates were correctly identified up to species level. Of species not represented in the database (e.g., Streptococcus suis and Bacillus spp.), 53.1% out of 49 isolates were incorrectly identified as non-related bacterial species. Testing of Gram-positive isolates on Gram-negative panels and vice versa (n = 144) resulted in incorrect identifications for 38.2% of tested isolates. The readability level of the IFU was considered too high for LRS. Inoculation of the panels was favorably evaluated, whereas the visual reading of the panels was considered error-prone. In conclusion, the accuracy of the MicroScan identification panels was excellent for Gram-negative species and good for Gram-positive species. Improvements in stability, robustness, and ease of use have been identified to assure adaptation to LRS constraints.

scholarly journals Involvement of cytokinins in adventitious and lateral root formation

Plant Root ◽  
2007 ◽  
Vol 1 ◽  
pp. 27-33 ◽  
Author(s):  
Takeshi Kuroha ◽  
Shinobu Satoh
Keyword(s):  
Lateral Root ◽  
Root Formation ◽  
Lateral Root Formation

scholarly journals Cross–talk between nitric oxide and Ca2+in elevated CO2-induced lateral root formation

2013 ◽  
Vol 8 (2) ◽  
pp. e23106 ◽  
Author(s):  
Huan Wang ◽  
Yaofang Niu ◽  
Rushan Chai ◽  
Miao Liu ◽  
Yongsong Zhang
Keyword(s):  
Nitric Oxide ◽  
Elevated Co2 ◽  
Cross Talk ◽  
Lateral Root ◽  
Root Formation ◽  
Lateral Root Formation

scholarly journals Potencial in vitro de Bacillus spp. no controle de Curtobacterium flaccumfaciens pv. flaccumfaciens em feijoeiro-comum

2016 ◽  
Vol 42 (4) ◽  
pp. 360-362
Author(s):  
Evelynne Urzêdo Leão ◽  
◽  
Júlio Cesar da Silva ◽  
Fabíola Rodrigues Medeiros ◽  
Gabriela Silva Santa Rosa Macêdo ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Bacillus Licheniformis ◽  
Bacillus Spp

RESUMO O feijoeiro-comum (Phaseolus vulgaris) é uma cultura de grande relevância na alimentação da população brasileira. A murcha-de-curtobacterium ou murcha bacteriana, causada por Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff). é uma doença vascular que acomete o feijoeiro causando danos significativos. Neste contexto, o objetivo deste trabalho foi avaliar a ação in vitro de Bacillus spp. na inibição de dois isolados de Cff, colonização do sistema radicular e desenvolvimento de plântulas de feijoeiro-comum. Foram realizados dois ensaios in vitro para verificar a atividade antagônica dos isolados Bacillus licheniformis, B. subtilis e B. subtilis + B. lichenformis a dois isolados de Cff. Todos os isolados de Bacillus spp. apresentaram inibição no crescimento dos isolados de Cff. Não foi observada a colonização das raízes das plântulas de feijoeiro-comum, pelos isolados bacterianos avaliados.

EXPERIMENTS ON THE RELATIVE SUSCEPTIBILITY OF WEEDS TO AUXIN HERBICIDES

1962 ◽  
Vol 40 (11) ◽  
pp. 1401-1409 ◽  
Author(s):  
J. R. Hay
Keyword(s):  
Phaseolus Vulgaris ◽  
Root Formation ◽  
Effective Control ◽  
Inorganic Salts ◽  
Dichlorophenoxyacetic Acid ◽  
Functional Importance ◽  
Molecular Configuration ◽  
Auxin Herbicides ◽  
Chlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid

Sections of stems of Silene cucubalus, Galeopsis tetrahit, and Phaseolus vulgaris were cultured under sterile conditions in media containing inorganic salts, sucrose, agar, and the herbicides 2-methyl, 4-chlorophenoxyacetic acid, 2,4-dichlorophenoxyacetic acid, or 2-(2,4,5-trichlorophenoxy)propionic acid. The herbicide which gave the most effective control of a species under field conditions was also the most active in inducing root formation and proliferation on stem sections of that species. The response of the sections to these auxin herbicides diminished as the plants from which the sections were taken became more mature. The amount of herbicide recovered from treated shoots of these species was not correlated with susceptibility. It is postulated that susceptibility to these materials depends upon (i) the affinity of the species for the molecular configuration of the herbicide and (ii) the relative amount and functional importance of the susceptible tissues at the time of treatment.

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