Physicochemical properties of PM-factor, a surface-active agent produced by Pseudomonas marginalis

1996 ◽  
Vol 42 (3) ◽  
pp. 243-251 ◽  
Author(s):  
G. Burd ◽  
O. P. Ward
Keyword(s):  
High Speed ◽  
Surface Active Agent ◽  
Gel Filtration ◽  
Active Agent ◽  
Aromatic Amino Acids ◽  
Polymyxin B ◽  
Culture Broth ◽  
Ultraviolet Absorption Spectrum ◽  
Emulsifying Activity ◽  
Surface Active

An extracellular surface-active agent, PM-factor, was obtained by high-speed centrifugation from the culture broth of Pseudomonas marginalis PD-14B. PM-factor exhibited emulsifying activity on a broad spectrum of hydrocarbon liquids, including aromatics, aliphatics, crude oil, and creosote. The factor appeared as ball-shaped particles of varying diameter when examined by electron microscopy (0.16–1.4 μm). Gel filtration chromatography demonstrated a high molecular mass of the factor (> 106 Da). The ultraviolet absorption spectrum manifested a peak in the region 200 nm rather in the region 260–280 nm. Amino acid analysis showed a very low amount of aromatic amino acids residues in the protein moiety of PM-factor. The presence of 3-deoxy-D-mannooctulosonic acid, heptose, hexosamine, phosphorus, and 3-hydroxy fatty acid indicated that PM-factor contained lipopolysaccharide. The emulsifying activity of PM-factor was inhibited strongly by mercuric chloride and moderately by EDTA. Polymyxin B, Ca2+, and Mg2+ markedly stimulated the factors emulsifying activity. Roles of the bioemulsifier in the functioning of P. marginalis as a plant pathogen and in bioremediation are discussed.Key words: bioemulsifier, Pseudomonas marginalis, polycyclic aromatic hydrocarbons, plant pathogenesis, bioremediation.

Adjunctive Use of a Surface-Active Agent in Extracorporeal Circulation

Circulation ◽  
1966 ◽  
Vol 33 (4s1) ◽  
Author(s):  
YOSHIMASA MIYAUCHI ◽  
TAKAYUKI INOUE ◽  
BRUCE C. PATON
Keyword(s):  
Extracorporeal Circulation ◽  
Surface Active Agent ◽  
Active Agent ◽  
Surface Active

Surface Active Agent Phenomena in Dust Abatement

1952 ◽  
Vol 44 (10) ◽  
pp. 2389-2393 ◽  
Author(s):  
P. L. Walker ◽  
E. E. Petersen ◽  
C. C. Wright
Keyword(s):  
Surface Active Agent ◽  
Active Agent ◽  
Surface Active

scholarly journals The Nature of the Muscle-Relaxing Factor

1963 ◽  
Vol 46 (5) ◽  
pp. 893-904 ◽  
Author(s):  
Franklin Fuchs ◽  
F. Norman Briggs
Keyword(s):  
Molecular Weight ◽  
High Speed ◽  
Gel Filtration ◽  
Active Agent ◽  
Calcium Deficiency ◽  
Chelating Resin ◽  
Prolonged Heating ◽  
Relaxing Factor ◽  
Chelex 100 ◽  
High Molecular Weight Material

High speed centrifugal fractionation of homogenates of rabbit skeletal muscle has led to the discovery of a soluble muscle-relaxing factor in the homogenate. Assay of the relaxing activity with deoxycholate-treated myofibrils and reconstituted actomyosin systems has established that the activity is not produced by the presence of contaminants. Relaxing activity could be removed or destroyed by charcoal, dialysis, prolonged heating, and treatment with the chelating resin, chelex-100, making it improbable that the effect is due simply to calcium deficiency. Many of the characteristics of this muscle-relaxing factor suggest that it is very similar to or the same as the factor formed by the incubation of muscle granule fractions and ATP. Evidence is presented that some soluble protein component is involved in the stabilization of the factor. The relaxing activity could be separated from the high molecular weight material in the supernatant by the technique of gel filtration. On the basis of the gel used, the molecular weight of the active agent should be less than 4000.

scholarly journals Study on Antimicrobial Surface Active Agent. III.

1975 ◽  
Vol 24 (2) ◽  
pp. 116-120
Author(s):  
Tsuyoshi UCHIBORI ◽  
Masatoshi TAKAHASHI ◽  
Ichiro YAMAMOTO ◽  
Shoichiro WATANABE
Keyword(s):  
Surface Active Agent ◽  
Active Agent ◽  
Surface Active
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