Isolation and description of carbazole-degrading bacteria

1996 ◽  
Vol 42 (1) ◽  
pp. 79-82 ◽  
Author(s):  
Joanna Shotbolt-Brown ◽  
David W. F. Hunter ◽  
Jackie Aislabie
Keyword(s):  
Substrate Specificity ◽  
Fossil Fuels ◽  
Bacterial Isolates ◽  
Enrichment Cultures ◽  
Plate Assay ◽  
Degrading Bacteria

Carbazole is a nitrogen hetrocyclic compound associated with fossil fuels and their products. Enrichment cultures were established to isolate bacteria able to degrade carbazole and a plate assay was developed to select carbazole-degrading microorganisms from the enrichments. Three different bacterial isolates capable of mineralizing carbazole were obtained. No intermediates of carbazole degradation were detected. The bacteria had a limited substrate specificity; all used benzoate for growth but were unable to utilize the analogues of carbazole, fluorene, or dibenzothiophene.Key words: carbazole, biodegradation, bacteria.

Biodegradation of organophosphorus Pollutants by Soil Bacteria: Biochemical Aspects and Unsolved Problems

2020 ◽  
Vol 36 (4) ◽  
pp. 126-135
Author(s):  
T.V. Shushkova ◽  
D.O. Epiktetov ◽  
S.V. Tarlachkov ◽  
I.T. Ermakova ◽  
A.A. Leontievskii
Keyword(s):  
Genome Sequencing ◽  
Soil Bacteria ◽  
Activity Regulation ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Russian Science ◽  
Degrading Bacteria ◽  
Phosphorus Source ◽  
Glyphosate Herbicide ◽  
Enzymatic Pathways

The degradation of persistent organophosphorus pollutants have been studied in 6 soil bacterial isolates and in 3 bacterial strains adapted for utilization of glyphosate herbicide (GP) under laboratory conditions. Significant differences in the uptake of organophosphonates were found in taxonomically close strains possessing similar enzymatic pathways of catabolism of these compounds, which indicates the existence of unknown mechanisms of activity regulation of these enzymes. The effect of adaptation for GP utilization as a sole phosphorus source on assimilation rates of several other phosphonates was observed in studied bacteria. The newly found efficient stains provided up to 56% of GP decomposition after application to the soil in the laboratory. The unresolved problems of microbial GP metabolism and the trends for further research on the creation of reliable biologicals capable of decomposing organophosphonates in the environment are discussed. organophosphonates, glyphosate, biodegradation, bioremediation, C-P lyase, phosphonatase, degrading bacteria Investigation of phosphonatase and genome sequencing were supported by Russian Science Foundation Grant no. 18-074-00021.

scholarly journals Broad diversity of bacteria degrading 17ß-estradiol-3-sulfate isolated from river sediment and biofilm at a wastewater treatment plant discharge

2021 ◽  
Author(s):  
Tamara Mainetti ◽  
Marilena Palmisano ◽  
Fabio Rezzonico ◽  
Blaž Stres ◽  
Susanne Kern ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
River Sediment ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Aquatic Environments ◽  
Bacterial Isolates ◽  
Degrading Bacteria ◽  
17Β Estradiol ◽  
Aquatic Wildlife ◽  
Harmful Effects

AbstractConjugated estrogens, such as 17β-estradiol-3-sulfate (E2-3S), can be released into aquatic environments through wastewater treatment plants (WWTP). There, they are microbiologically degraded into free estrogens, which can have harmful effects on aquatic wildlife. Here, the degradation of E2-3S in environmental samples taken upstream, downstream and at the effluent of a WWTP was assessed. Sediment and biofilm samples were enriched for E2-3S-degrading microorganisms, yielding a broad diversity of bacterial isolates, including known and novel degraders of estrogens. Since E2-3S-degrading bacteria were also isolated in the sample upstream of the WWTP, the WWTP does not influence the ability of the microbial community to degrade E2-3S.

Isolation and Characterization of Cellulose Degrading Bacteria of Termite Gut from North Eastern Region of India

2016 ◽  
Vol 5 (6) ◽  
pp. 283-290
Author(s):  
Sonika Sharma ◽  
Soumya Chatterjee ◽  
Sibnarayan Datta ◽  
Rajesh Kumar Prasad ◽  
Angkita Sharma ◽  
...  
Keyword(s):  
Agar Plate ◽  
Bacterial Consortium ◽  
Cellulase Production ◽  
Eastern Region ◽  
Bacterial Isolates ◽  
Carbohydrate Utilization ◽  
North East ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Termite Gut

A study was conducted to screen and isolate cellulase producing bacteria of termite gut from North east region of India. A total of 27 culturable bacterial isolates were screened for cellulase production. Out of the 27 bacterial isolates 11 showed zone of clearance on CMC agar media on staining with 1% Congo red, suggesting potential cellulose degrading activity. The maximum hydrolysis capacities (HC value) on CMC agar plate was found within the range of 3.6 to 40mm. The morphological characterization and gram staining of the positive isolates indicated that 3 isolates were positively stained rods and others were negative cocci. All the cellulase positive isolates were also tested for carbohydrate utilization with maltose, dextrose and fructose, to which all the 11 isolates responded positively. Further, based on the amplification and sequencing of the 16S rRNA genetic region, isolates were identified as member of the genus Bacillus, Paenibacillus and Staphylococcus. The degrading potential of these bacteria were assessed by developing bacterial consortium and efficient degradation was reported after seven days of incubation with different cellulose source like rice, cotton and rice husk.

scholarly journals Isolation and Identification of Hydrocarbon-Degrading Bacteria that Tolerant to Saponin of Sapindus Rarak Plant

2019 ◽  
Vol 4 (1) ◽  
pp. 79-88
Author(s):  
Evi Octaviany ◽  
Suharjono Suharjono ◽  
Irfan Mustafa
Keyword(s):  
Crude Oil ◽  
16S Rdna ◽  
Bacterial Isolates ◽  
High Concentration ◽  
Isolation And Identification ◽  
Rdna Sequences ◽  
Degrading Bacteria ◽  
16S Rdna Sequences ◽  
Petroleum Contaminated Soil ◽  
Cell Densities

A commercial saponin as biosurfactant can reduce the surface tension of water and increase of hydrocarbon degradation. However, this saponin can be toxic to some hydrocarbonoclastic bac-teria. This study aimed to obtain bacterial isolates that were tolerant and incapable to degrade saponin, and to identify them based on 16S rDNA sequence. Bacteria were isolated from petroleum contaminated soil in Wonocolo Village, Bojonegoro Regency, East Java, Indonesia. The soil samples were acclimated using Bushnell-Haas (BH) broth with 0.5% crude oil at room temperature for 3 weeks. The culture was spread onto BH agar incubated at 30°C for 7 days. The first screened, isolates were grown in nutrient broth with addition of sap-onin 0%, 8%, and 12% (v/v) then incubated at 30°C for three days. The bacterial cell density was measured using a spectrophotometer. Second screened, the isolates were grown on BH broth with addition of 0.5% saponin as a sole carbon source, and their cell densities were measured. The selected isolates were identified based on 16S rDNA sequences. Among 34 bacterial isolates, nine isolates were tol-erant to 12% saponin. Three bacterial isolates IHT1.3, IHT1.5, and IHT3.24 tolerant to high concentration of saponin and did not use this substance as growth nutrition. The IHT1.3, IHT1.5, and IHT3.24 isolates were identified as Ochrobactrum pseudogrignonense (99% similarity), Pseudomonas mendocina (99%), and Ochrobactrum pi-tuitosum; (97%), respectively. Those three selected isolates are good candidates as hydrocarbon-degrading bacteria to bioremediation of soil contaminated crude oil. However, the combined activity of bacteria and saponin to degrade hydrocarbon needs further study. 

scholarly journals Isolation of Polymer-Degrading Bacteria and Characterization of the Hindgut Bacterial Community from the Detritus-Feeding Larvae of Tipula abdominalis (Diptera: Tipulidae)

2007 ◽  
Vol 73 (17) ◽  
pp. 5683-5686 ◽  
Author(s):  
Dana M. Cook ◽  
Emily DeCrescenzo Henriksen ◽  
Rima Upchurch ◽  
Joy B. Doran Peterson
Keyword(s):  
Microbial Community ◽  
Initial Study ◽  
Gene Clone ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Future Studies ◽  
Degrading Bacteria ◽  
Novel Bacteria ◽  
Tipula Abdominalis

ABSTRACT The Tipula abdominalis larval hindgut microbial community presumably facilitates digestion of the lignocellulosic diet. The microbial community was investigated through characterization of bacterial isolates and analysis of 16S rRNA gene clone libraries. This initial study revealed novel bacteria and provides a framework for future studies of this symbiosis.

Biodegradation of Haloacetic Acids by Bacterial Isolates and Enrichment Cultures from Drinking Water Systems

2009 ◽  
Vol 43 (9) ◽  
pp. 3169-3175 ◽  
Author(s):  
Ping Zhang ◽  
Timothy M. LaPara ◽  
Emma H. Goslan ◽  
Yuefeng Xie ◽  
Simon A. Parsons ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Systems ◽  
Haloacetic Acids ◽  
Bacterial Isolates ◽  
Enrichment Cultures ◽  
Drinking Water Systems

scholarly journals Isolation, Characterization, and Identification of Bacterial Population from Textile and Tannery Effluents of Bangladesh

2016 ◽  
Vol 29 (2) ◽  
pp. 84-88
Author(s):  
A Hakim ◽  
S Hoque ◽  
SM Ullah
Keyword(s):  
Bacterial Population ◽  
Bacterial Species ◽  
Bacterial Count ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Tannery Effluents ◽  
Bacterial Composition ◽  
Degrading Bacteria ◽  
Diagnostic Characteristics ◽  
Metal Treatment

Ten effluent samples from two different sites located at Hazaribagh tannery belt and Dhaka EPZ, Savar were collected. This study aimed to compare the bacterial composition isolated from tannery and textile effluents and to investigate the occurrence of metal toxicity tolerant and dye degrading bacteria and to select the potential strains for the use in bioremediation. The average bacterial count of HT and DETDE varied in between 3.35×106 and 5.45×106 cfu/mL and 4.8×106 and 7.75×106cfu/mL, respectively. A total of 12 bacterial isolates were characterized as strains of Bacillus, Staphylococcus, and Pseudomonas. A few, however, were re-cultured on other recommended media for verification of diagnostic characteristics. Maximum numbers of bacterial species were isolated from textile effluent. The results showed that a Gram-positive bacillus with a yellow pigment was considered as a major group of the population. Among them three isolates were identified based on alignments of partial sequence of 16S rRNA gene. These are also being used in different wastewater and metal treatment plants all over the world.Bangladesh J Microbiol, Volume 29, Number 2, Dec 2012, pp 84-88

scholarly journals Isolation and Identification of Novel Microcystin-Degrading Bacteria

2009 ◽  
Vol 75 (21) ◽  
pp. 6924-6928 ◽  
Author(s):  
Pathmalal M. Manage ◽  
Christine Edwards ◽  
Brajesh K. Singh ◽  
Linda A. Lawton
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
Bacterial Isolates ◽  
Isolation And Identification ◽  
First Report ◽  
Degrading Bacteria

ABSTRACT Of 31 freshwater bacterial isolates screened using the Biolog MT2 assay to determine their metabolism of the microcystin LR, 10 were positive. Phylogenetic analysis (16S rRNA) identified them as Arthrobacter spp., Brevibacterium sp., and Rhodococcus sp. This is the first report of microcystin degraders that do not belong to the Proteobacteria.

Substrate specificity of chlorophenoxyalkanoic acid-degrading bacteria is not dependent upon phylogenetically related tfdA gene types

2001 ◽  
Vol 33 (6) ◽  
pp. 507-513 ◽  
Author(s):  
Christopher Smejkal ◽  
Tatiana Vallaeys ◽  
Sara Burton ◽  
Hilary Lappin-Scott
Keyword(s):  
Substrate Specificity ◽  
Degrading Bacteria ◽  
Tfda Gene

scholarly journals The Isolation and identification of cellulolytic bacteria at fibric, hemic and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia

2020 ◽  
Vol 21 (5) ◽  
Author(s):  
Siti Khotimah ◽  
Suharjono Suharjono ◽  
Tri Ardyati ◽  
Yulia Nuraini
Keyword(s):  
Organic Matter ◽  
Oil Palm ◽  
Secondary Forest ◽  
Bacterial Isolates ◽  
Cellulolytic Bacteria ◽  
Isolation And Identification ◽  
Plate Method ◽  
Cellulase Enzyme ◽  
Degrading Bacteria ◽  
Relationship Of

Abstract. Khotimah S, Suharjono, Ardyati T, Nurani Y. 2020. Isolation and identification of cellulolytic bacteria at fibric, hemic, and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia. Biodiversitas 21: 2103-2112. Cellulose degrading bacteria was one of the microbial removers of organic matter contained in the soil into simpler monomers so that it can be utilized by other organisms. The objective of the research was to obtain cellulose-degrading bacteria found on fibric, hemic, and sapric peat in forest and shrubs (oil palm). The bacteria were isolated by pour plate method on 1% CMC media. Selected isolates were assayed quantitatively based on the activity of cellulase enzyme, identified with 16S rDNA. The density of cellulolytic bacteria in the secondary forest peat of fibric, hemic, sapric were 2.1x103 cfu/g, 5.9x104 cfu/g, and 4.9x104 cfu/g whereas, in the area of shrubs/oil palm peat fibric, hemic and sapric 6.9x104 cfu/g, 8.4x104 cfu/g and 3.4x105 cfu/g respectively. There were 19 bacterial isolates that have clear zones around the colony as degradation of cellulose had highest ability to degrade cellulose with clear zones of 5-7 mm. The strain of SB1.1.1 showed highest activity of cellulase enzyme 11.17 U/mL, followed by HH3.1.1 strain and SB2.3 7.83 U/mL. Based on the phylogeny tree, strain SB1.1.1 and HH3.1.1 have the closest kinship relationship with Bacillus cereus with a kinship relationship of 100%, while SB2.3 has the closest kinship relationship with Bacillus stratosphericus with a relationship of 99.85 %.

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