Molecular techniques and their potential application in monitoring the microbiological quality of indoor air

1995 ◽  
Vol 41 (8) ◽  
pp. 657-665 ◽  
Author(s):  
L. MacNeil ◽  
T. Kauri ◽  
W. Robertson
Keyword(s):  
Indoor Air ◽  
Molecular Detection ◽  
Microbiological Quality ◽  
Air Sampling ◽  
Restriction Endonuclease Analysis ◽  
Molecular Techniques ◽  
Detection Methods ◽  
Individual Species ◽  
Bacteria And Fungi

Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This review discusses the shortcomings of current methods of identification and detection and focuses on the potential for molecular techniques in this emerging field. Probe techniques, restriction endonuclease analysis, karyotyping, and DNA and polymerase chain reaction fingerprinting methods available to detect and identify bacteria and fungi significant in the indoor air environment are discussed. Problems that may be encountered using these techniques are also considered. The authors have included a brief discussion on current air sampling techniques as well as adapting these techniques for use with molecular detection methods.Key words: indoor air microbiology, microbiological air sampling, molecular detection methods.

Microflora of smoked Trachurus trachurus and Scomber scombrus samples in Orlu South East, Nigeria and its implication on public health

2020 ◽  
Vol 34 (1) ◽  
pp. 7-14
Author(s):  
L.A. Agbabiaka ◽  
C.O. Agu
Keyword(s):  
Public Health ◽  
Microbiological Quality ◽  
Viable Count ◽  
Trachurus Trachurus ◽  
Salmonella Spp ◽  
Candida Spp ◽  
Fish Samples ◽  
Bacteria And Fungi ◽  
And Staphylococcus Aureus

The study was carried out to assess the microbiological quality of smoked Trachurus trachurus and Scomber scumbrus in four markets in Orlu Local Government Area, South East, Nigeria. Forty freshly smoked samples of Trachurus trachurus and Scomber scumbrus were procured from Eke Eziachi, Orlu Main, Aforaja Umuowa and Afor Ogidi Ihioma markets and analyzed using standard techniques. Results revealed that Orlu main market had the highest level of microbial contamination (mean bacteria and fungi count of 5 x 102 and 10 x 102cfu/g respectively). The highest total viable count (7 x102 cfu/g) of Scomber scumbrus was obtained in Aforaja Umuowa market while the least value of 2 x102 cfu/g was observed in Afor Ogidi Ihioma market. Also, similar value was recorded in Trachurus trachurus at Aforaja Umuowa market. Results from the study indicated that pathogens present in fish samples included bacteria (Bacillus cereus, B. subtilis, Escherichia coli, Salmonella spp and Staphylococcus aureus) and fungi (Gliocladium spp., Candida spp., Rhizopus spp., Alternaria spp., Aspergillus spp., Clasdosprium spp., Fonsecaca spp., Chrysoporius spp., mucour spp. and Botrytis spp). The study also showed that freshly smoked Trachurus trachurus and Scomber scumbrus samples sold in the four markets under assessment were contaminated with pathogenic organisms. This may however present some public health implications. Keywords: Microbial activity, Smoked fish, Orlu, Fish quality; Hygiene

Environmental microbiology

2017 ◽  
Vol 2 (11) ◽  
Author(s):  
Felicita Briški ◽  
Marija Vuković Domanovac
Keyword(s):  
Indoor Air ◽  
Hot Springs ◽  
Microbiological Quality ◽  
Environmental Microbiology ◽  
Toxic Substances ◽  
Biological Degradation ◽  
Dominant Form ◽  
Form Of Life ◽  
Complex Organic

Abstract For most people, microorganisms are out of sight and therefore out of mind but they are large, extremely diverse group of organisms, they are everywhere and are the dominant form of life on planet Earth. Almost every surface is colonized by microorganisms, including our skin; however most of them are harmless to humans. Some microorganisms can live in boiling hot springs, whereas others form microbial communities in frozen sea ice. Among their many roles, microorganisms are necessary for biogeochemical cycling, soil fertility, decomposition of dead plants and animals and biodegradation of many complex organic compounds present in the environment. Environmental microbiology is concerned with the study of microorganisms in the soil, water and air and their application in bioremediation to reduce environmental pollution through the biological degradation of pollutants into non-toxic or less toxic substances. Field of environmental microbiology also covers the topics such as microbially induced biocorrosion, biodeterioration of constructing materials and microbiological quality of outdoor and indoor air.

scholarly journals Application of chemometric methods for assessment and modelling of microbiological quality data concerning coastal bathing water in Greece

2014 ◽  
Vol 3 (3) ◽  
Author(s):  
Agelos Papaioannou ◽  
George Rigas ◽  
Panagiotis Papastergiou ◽  
Christos Hadjichristodoulou
Keyword(s):  
Cluster Analysis ◽  
Large Scale ◽  
Microbiological Quality ◽  
Terrestrial Ecosystems ◽  
Detection Methods ◽  
Quality Data ◽  
Waterborne Diseases ◽  
Chemometric Methods ◽  
Bathing Water

<em>Background</em>. Worldwide, the aim of managing water is to safeguard human health whilst maintaining sustainable aquatic and associated terrestrial, ecosystems. Because human enteric viruses are the most likely pathogens responsible for waterborne diseases from recreational water use, but detection methods are complex and costly for routine monitoring, it is of great interest to determine the quality of coastal bathing water with a minimum cost and maximum safety. <br /><em>Design and methods.</em> This study handles the assessment and modelling of the microbiological quality data of 2149 seawater bathing areas in Greece over 10-year period (1997-2006) by chemometric methods. <br /><em>Results</em>. Cluster analysis results indicated that the studied bathing beaches are classified in accordance with the seasonality in three groups. Factor analysis was applied to investigate possible determining factors in the groups resulted from the cluster analysis, and also two new parameters were created in each group; VF1 includes <em>E. coli</em>, faecal coliforms and total coliforms and VF2 includes faecal streptococci/enterococci. By applying the cluster analysis in each seasonal group, three new groups of coasts were generated, group A (ultraclean), group B (clean) and group C (contaminated). <em>Conclusions</em>. The above analysis is confirmed by the application of discriminant analysis, and proves that chemometric methods are useful tools for assessment and modeling microbiological quality data of coastal bathing water on a large scale, and thus could attribute to effective and economical monitoring of the quality of coastal bathing water in a country with a big number of bathing coasts, like Greece.

scholarly journals Detection of drug resistant bacteria in different ice creams available in local market of Dhaka city

2017 ◽  
Vol 7 (1) ◽  
pp. 33-35
Author(s):  
Md Musa Howlader ◽  
Tahmina Shammi
Keyword(s):  
Staphylococcus Aureus ◽  
Microbiological Quality ◽  
Ice Cream ◽  
Storage Condition ◽  
Resistant Bacteria ◽  
Local Market ◽  
Drug Resistant ◽  
Drug Resistant Bacteria ◽  
Bacteria And Fungi

Sound microbiological quality of ice cream should be ensured for being a widely popular dairy food in the world. Present study was conducted to determine the microbiological quality of different ice cream samples available in Dhaka, Bangladesh. Total 3 ice cream samples were collected and processed to detect the microbiological quality as well as drug resistant trait of the isolates through several conventional Kirby Bauer method. All the samples were found to be contaminated with the total viable bacteria and fungi within the range of 1.2×104 cfu/ml to 4.3×106 cfu/ml. The presence of E. coli, Staphylococcus aureus was also observed up to 104 cfu/ml. However, the fecal contamination was totally absent in all the samples. Antibiotic profile of two isolates was measured against 8 commonly used antibiotics and both E.coli and Staphylococcus aureus were found to be resistant against more than one antibiotics. Appropriate hygienic and storage condition should be maintained concerning the health safety of consumers. Stamford Journal of Microbiology, Vol.7(1) 2017: 33-35

scholarly journals Microbial Assessment of Indoor Air Quality of Ventilation Systems

2019 ◽  
pp. 1-7
Author(s):  
H. O. Stanley ◽  
B. Onwuna ◽  
C. J. Ugboma
Keyword(s):  
Indoor Air ◽  
Natural Ventilation ◽  
Airborne Bacteria ◽  
Fungal Isolates ◽  
Enterococcus Spp ◽  
Bacillus Spp ◽  
Bacteria And Fungi ◽  
Klebsiella Spp

The aim of the study was to compare the indoor levels of airborne bacteria and fungi of air conditioned (AC) buildings and naturally ventilated (NV) buildings using a Supermarket, a Laboratory and an Eatery as a case study. The predominant bacterial isolates were Staphylococcus, Micrococcus spp., Escherichia coli, Bacillus spp., Streptococcus spp., Enterococcus spp., Klebsiella spp. The fungal isolates belong to the genera Penicillium, Aspergillus, Mucor, Trichophyton, Fusarium, Candida and Chaetomium. The levels of airborne bacteria and fungi were determined using settle plate method. In AC buildings the average air levels of bacteria (supermarket: 24.2 CFU m-3; laboratory: 29.2 CFU m-3; eatery: 51.0 CFU m-3 air) were higher than in NV (respectively: 54.3 CFU m-3; 100.7 CFU m-3; 134.3 CFU m-3 air). The average air levels for fungal isolates were higher in the eatery due to presence of poorly maintained AC system (supermarket: 7.8 CFU m-3; laboratory: 11.5 CFU m-3; eatery: 56.7 CFU m-3 air) than in NV (28.6 CFU m-3; 19.6 CFU m-3; 13.5 CFU m-3 air respectively). Findings from this study showed that AC buildings had lower levels of bacterial and fungal contamination compared to buildings with natural ventilation.

scholarly journals Microbiological analysis of pasteurized and fresh fruit juice sold in Rawalpindi of Pakistan

2013 ◽  
Vol 48 (3) ◽  
pp. 185-192 ◽  
Author(s):  
SA Batool ◽  
SS Tahir ◽  
N Rauf ◽  
R Kalsoom
Keyword(s):  
Fruit Juice ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Viable Count ◽  
Fruit Juices ◽  
Local Market ◽  
Microbiological Analysis ◽  
E Coli ◽  
Bacteria And Fungi

Freshly prepared and pasteurized fruit juices sold by vendors in local market of Rawalpindi city from 10 locations were analyzed for the microbiological quality. Total viable count (TVC), total coliform, faecal coliform, molds and the presence of pathogenic microorganisms such as E. coli, Pseudomonas, Staphylococcus aureus, Salmonella, and fungi like Aspergillus, Pencillum, Rhizopus were determined .In open fruit juices available in city were highly contaminated with bacteria and fungi. E. coli, Salmonella, Staphylococcus, and Pseudomonas were isolated with different frequency. Aspergillus, Pencillum and Rhizopus were also found in juices especially Aspergillus was with high percentages. The pasteurized juices have less contamination as compared to the fresh juices samples. The number and type of microorganisms recovered from the freshly squeezed fruit juices made them unsafe for drinking. The results of this study demonstrate the unhygienic quality of popular types of market vended freshly squeezed fruit juices and their risk to the consumers. DOI: http://dx.doi.org/10.3329/bjsir.v48i3.17329 Bangladesh J. Sci. Ind. Res. 48(3), 185-192, 2013

scholarly journals Microbiological Quality of Indoor Air in University Libraries

2014 ◽  
Vol 4 ◽  
pp. S312-S317 ◽  
Author(s):  
Samuel Fekadu Hayleeyesus ◽  
Abayneh Melaku Manaye
Keyword(s):  
Indoor Air ◽  
Microbiological Quality ◽  
University Libraries

scholarly journals Detection of Non-Alcoholic Beer Spoilage Microorganisms at Critical Points of Production by Polymerase Chain Reaction

2020 ◽  
Vol 11 (2) ◽  
pp. 9658-9668
Keyword(s):  
Polymerase Chain Reaction ◽  
Microbiological Quality ◽  
Detection Methods ◽  
Specific Method ◽  
Chain Reaction ◽  
Critical Control Points ◽  
Beer Spoilage ◽  
Polymerase Chain ◽  
Yeasts And Molds

Detection of non-alcoholic beer contaminants is complicated as lengthy incubation time is required for cultivation; indeed, this is a need for rapid detection methods. A recent study was conducted to determine the microbiological quality of non-alcoholic beers of four brads (A-D) in Tehran, Iran. Polymerase chain reaction (PCR) is applied as a rapid and specific method for the detection of contaminants in beer manufacturing. The PCR protocols were effective for the detection of yeasts in beers during processing until packaging. The presence of wild yeasts and molds during processing could be risky and will affect the final product quality. The predominant contaminants of non-alcoholic beer were found to be the species of Saccharomyces, Pichia, Rhodotorula, Alternaria, Hansenia, Wickerhamomyces, and Cladosporium. It is essential to inform the producers about hazards, and critical control points in non-alcoholic beer processing stages, including; wort boiling, clarification, filtration, and packaging, and the implication of environmental hygiene are deniable.

scholarly journals Microbiological indoor air quality in faculty's rooms: Risks on students' health

2021 ◽  
pp. 63-72
Author(s):  
Olgica Stefanović ◽  
Jelena Radosavljević ◽  
Marijana Kosanić
Keyword(s):  
Air Quality ◽  
Qualitative Analysis ◽  
Indoor Air Quality ◽  
Human Skin ◽  
Indoor Air ◽  
Microbiological Quality ◽  
Skin Microbiota ◽  
School Year ◽  
High Level

This study deals with a quantitative and qualitative analysis of indoor airborne microbiota and estimation of microbiological quality of indoor air in faculty's rooms during the summer semester of 2017/18 school year. The concentration of bacteria was significantly higher than the concentration of fungi. The species that belong to human skin microbiota or of environmental origin were identified. According to indoor air quality breakpoints, low to medium/high level of bacterial and fungal air contaminations was noticed.

scholarly journals Use of molecular techniques for the analysis of the microbiological quality of fish marketed in the municipality of Cuiabá, Mato Grosso, Brazil

2019 ◽  
Vol 39 (suppl 1) ◽  
pp. 146-151
Author(s):  
Débora Cristina PASTRO ◽  
Sandra MARIOTTO ◽  
Erika Cerqueira SANTOS ◽  
Daniela Cristina FERREIRA ◽  
Gilma Silva CHITARRA
Keyword(s):  
Microbiological Quality ◽  
Molecular Techniques ◽  
Mato Grosso
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