Construction of a new shuttle vector for Lactobacillus

1992 ◽  
Vol 38 (1) ◽  
pp. 69-74 ◽  
Author(s):  
P. Chagnaud ◽  
C. K. N. Chan Kwo Chion ◽  
R. Duran ◽  
P. Naouri ◽  
A. Arnaud ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Selection Pressure ◽  
Shuttle Vector ◽  
Restriction Enzymes ◽  
Lactobacillus Bulgaricus ◽  
Restriction Map ◽  
Enzyme Gene ◽  
Shuttle Plasmid ◽  
Malolactic Enzyme ◽  
Mrs Medium

To clone the malolactic enzyme gene from Lactobacillus sp. 89, construction of a shuttle vector able to express itself in Lactobacillus sp. 89 and Escherichia coli was undertaken. The shuttle plasmid pLE16 resulted from the union of pBR328 and of the pLB10 plasmid extracted from Lactobacillus bulgaricus 10. The bacterial transformation in Lactobacillus sp. 89 was performed by electroporation, and the clones were selected on MRS medium with 30 μg∙mL−1 chloramphenicol added. Fifty percent of the clones from Lactobacillus sp. 89 lost their resistance to chloramphenicol following 28 generations when the selection pressure was not maintained. The restriction map of pLE16 (7600 bp) was established using several restriction enzymes. Key words: malolactic enzyme, shuttle plasmid, Escherichia coli, Lactobacillus, electroporation.

scholarly journals Characterization of the Basic Replicon ofRhodococcus Plasmid pSOX and Development of aRhodococcus-Escherichia coli Shuttle Vector†

1998 ◽  
Vol 64 (11) ◽  
pp. 4363-4367 ◽  
Author(s):  
Claude Denis-Larose ◽  
Hélène Bergeron ◽  
Diane Labbé ◽  
Charles W. Greer ◽  
Jalal Hawari ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shuttle Vector ◽  
Open Reading Frames ◽  
Chloramphenicol Resistance ◽  
Rep Protein ◽  
E Coli ◽  
Shuttle Plasmid ◽  
Rep Proteins ◽  
Replication Region ◽  
Internal Initiation

ABSTRACT The replication region of a 100-kb desulfurization plasmid (pSOX) from Rhodococcus sp. strain X309 was localized to a 4-kbKpnI fragment, and its sequence was determined. The amino acid sequence of one of the predicted open reading frames (ORFs) was related to the putative replication (Rep) protein sequences of the mycobacterial pLR7 family of plasmids. Three of the five predicted ORF products were identified by radiolabelling with the Escherichia coli T7 polymerase/promoter system. In E. coli, the Rep protein of pSOX was apparently synthesized in a shortened form, 21.3 kDa instead of the predicted 41.3 kDa, as a result of an internal initiation. This situation is reminescent of that for some bacterial Rep proteins. A shuttle plasmid was constructed with the pSOX origin, pBluescript II KS−, and the chloramphenicol resistance (Cmr) gene from pRF29. This new shuttle plasmid was used to demonstrate expression of the Bacillus subtilis sacB gene in a strain of Rhodococcus, rendering it sensitive to the presence of sucrose.

scholarly journals Alignment of Escherichia coli K12 DNA sequences to a genomic restriction map

1990 ◽  
Vol 18 (2) ◽  
pp. 313-321 ◽  
Author(s):  
Kenneth E. Rudd ◽  
Webb Miller ◽  
James Ostell ◽  
Dennis A. Benson
Keyword(s):  
Escherichia Coli ◽  
Dna Sequences ◽  
Restriction Map ◽  
Escherichia Coli K12

Restriction map of the hepatitis B virus DNA cloned in Escherichia coli

Gene ◽  
1982 ◽  
Vol 20 (3) ◽  
pp. 481-484 ◽  
Author(s):  
V.V. Bichko ◽  
T.M. Kozlovskaya ◽  
A. Dishler ◽  
P. Pumpen ◽  
A. Janulaitis ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Restriction Map ◽  
Hepatitis B Virus Dna ◽  
B Virus

scholarly journals A Wall of Funnels Concentrates Swimming Bacteria

2007 ◽  
Vol 189 (23) ◽  
pp. 8704-8707 ◽  
Author(s):  
Peter Galajda ◽  
Juan Keymer ◽  
Paul Chaikin ◽  
Robert Austin
Keyword(s):  
Escherichia Coli ◽  
Porous Media ◽  
Biological Tissue ◽  
Selection Pressure ◽  
Random Motion ◽  
Swimming Bacteria ◽  
Multistage Pump ◽  
Narrow Opening ◽  
Arbitrary Shapes ◽  
Motile Bacteria

ABSTRACT Randomly moving but self-propelled agents, such as Escherichia coli bacteria, are expected to fill a volume homogeneously. However, we show that when a population of bacteria is exposed to a microfabricated wall of funnel-shaped openings, the random motion of bacteria through the openings is rectified by tracking (trapping) of the swimming bacteria along the funnel wall. This leads to a buildup of the concentration of swimming cells on the narrow opening side of the funnel wall but no concentration of nonswimming cells. Similarly, we show that a series of such funnel walls functions as a multistage pump and can increase the concentration of motile bacteria exponentially with the number of walls. The funnel wall can be arranged along arbitrary shapes and cause the bacteria to form well-defined patterns. The funnel effect may also have implications on the transport and distribution of motile microorganisms in irregular confined environments, such as porous media, wet soil, or biological tissue, or act as a selection pressure in evolution experiments.

scholarly journals Establishment of a Tractable Genetic Transformation System in Veillonella spp.

2012 ◽  
Vol 78 (9) ◽  
pp. 3488-3491 ◽  
Author(s):  
Jinman Liu ◽  
Zhoujie Xie ◽  
Justin Merritt ◽  
Fengxia Qi
Keyword(s):  
Escherichia Coli ◽  
Genetic Transformation ◽  
Shuttle Vector ◽  
Transformation System ◽  
Genetic Studies ◽  
Content Type ◽  
Genetic Transformation System

ABSTRACTWe have constructed the firstEscherichia coli-Veillonellashuttle vector based on an endogenous plasmid (pVJL1) isolated from a clinicalVeillonellastrain. A highly transformableVeillonellastrain was also identified. Both the shuttle vector and the transformable strain should be valuable tools for futureVeillonellagenetic studies.

Nucleotide sequence of the replication region from the Mycobacterium-Escherichia coli shuttle vector pEP2

Gene ◽  
1990 ◽  
Vol 96 (1) ◽  
pp. 147-148 ◽  
Author(s):  
Linda J. Messerotti ◽  
Anthony J. Radford ◽  
Adrian L.M. Hodgson
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Shuttle Vector ◽  
Replication Region
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