Characterization of interspecific hybrids within the Aspergillus nidulans group by isoenzyme analysis

1991 ◽  
Vol 37 (5) ◽  
pp. 391-396 ◽  
Author(s):  
Éva Tóth Kálmán ◽  
János Varga ◽  
Ferenc Kevei
Keyword(s):  
Aspergillus Nidulans ◽  
Interspecific Hybrids ◽  
Species Group ◽  
Chromosome Loss ◽  
Isoenzyme Analysis ◽  
Electrophoretic Patterns ◽  
Isoenzyme Composition ◽  
Key Words Aspergillus ◽  
Isoenzyme Patterns

A comparison of interspecific hybrids within the Aspergillus nidulans species group was made by isoenzyme analysis. The gel electrophoretic patterns of the parental species were distinct for most of the enzymes tested. The hybrids were distinguishable from their parents by isoenzyme patterns. The appearance of novel bands in all the interspecific hybrids indicated that nuclear fusion could have occurred. In most hybrids the appearance of the parental bands showed nonpreferential, partial chromosome loss or repressive interaction between the genomes. The isoenzyme composition of the haploid segregants of the Aspergillus nidulans × Aspergillus rugulosus hybrid differed for some of the enzymes studied from that of the hybrid, suggesting that during segregation further interaction of the chromosomes took place. The results indicate a certain degree of genetic homology among the members of the Aspergillus nidulans species group. Key words: Aspergillus, isoenzyme analysis, hybridization.

Characterization of interspecific hybrids of Triticum aestivum x Aegilops sp. without 5B chromosome for induced homoeologous pairing

2015 ◽  
Vol 25 (1) ◽  
pp. 117-120 ◽  
Author(s):  
Imran Sheikh ◽  
Prachi Sharma ◽  
Shailender Kumar Verma ◽  
Satish Kumar ◽  
Sachin Malik ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Interspecific Hybrids ◽  
Homoeologous Pairing ◽  
Induced Homoeologous Pairing

Revision of the Metallactus kollari species-group with a new diagnosis of the genus (Coleoptera: Chrysomelidae: Cryptocephalinae)

Zootaxa ◽  
2018 ◽  
Vol 4413 (1) ◽  
pp. 57
Author(s):  
DAVIDE SASSI
Keyword(s):  
Type Species ◽  
Species Group ◽  
Identification Key ◽  
Original Diagnosis ◽  
Taxonomic Treatment ◽  
Species Groups ◽  
New Diagnosis ◽  
Good Resource

The genus Metallactus has been controversial since its introduction due to the ambiguous nature of the original diagnosis. This has caused some confusion in the taxonomy of Neotropical Pachybrachina. In this work the morphology of endophallus, which is useful for the characterization of species groups in several groups of Coleoptera, including Cryptocephalinae, has been analyzed. This has proven to be a good resource also in the taxonomic treatment of the species belonging to the genus Metallactus. After a careful survey on most of the species described so far, the endophallus shape in Metallactus turned out to be remarkably distinctive and very promising in the delimitation of species groups. The present work includes: a) a new diagnosis of the genus Metallactus on the basis of the aedeagal anatomy; b) the designation of the type species of the nominal genus; c) the revision of a first species-group of the genus, including the type species, hereinafter called Metallactus kollari species-group. Before this revision, catalogues had been reporting 13 species attributable to this group, in the present work three species have been synonymized and seven have been described as new to science. Therefore, the group includes 17 species. The species described as new are: Metallactus rileyi n. sp., M. bellatrix n. sp., M. longicornis n. sp.; M. londonpridei n. sp., M. regalini n. sp., M. bezoar n. sp., M. guarani n. sp. The new synonymies are as follows: Metallactus albipes Suffrian, 1866 (= M. nigrofasciatus Suffrian, 1866 n. syn.), M. albifrons Suffrian, 1866 (= M. flavofrontalis Jacoby, 1907 n. syn.), M. dodecastictus Suffrian, 1866 (= Griburius nigritarsis Jacoby, 1907 n. syn.). The lectotypes of all previously described species have been designated. The new synonymies, the name-bearing type fixations and designations and the nomenclatural acts have been critically discussed. An identification key for the species-group is also provided. 

Characterization of the Role of the FluG Protein in Asexual Development of Aspergillus nidulans

Genetics ◽  
2001 ◽  
Vol 158 (3) ◽  
pp. 1027-1036 ◽  
Author(s):  
Cletus A D'Souza ◽  
Bee Na Lee ◽  
Thomas H Adams
Keyword(s):  
Aspergillus Nidulans ◽  
Negative Influence ◽  
Asexual Development ◽  
Wild Type ◽  
Significant Similarity ◽  
Developmental Defects ◽  
Asexual Sporulation ◽  
Type Strains

Abstract We showed previously that a ΔfluG mutation results in a block in Aspergillus nidulans asexual sporulation and that overexpression of fluG activates sporulation in liquid-submerged culture, a condition that does not normally support sporulation of wild-type strains. Here we demonstrate that the entire N-terminal region of FluG (∼400 amino acids) can be deleted without affecting sporulation, indicating that FluG activity resides in the C-terminal half of the protein, which bears significant similarity with GSI-type glutamine synthetases. While FluG has no apparent role in glutamine biosynthesis, we propose that it has an enzymatic role in sporulation factor production. We also describe the isolation of dominant suppressors of ΔfluG(dsg) that should identify components acting downstream of FluG and thereby define the function of FluG in sporulation. The dsgA1 mutation also suppresses the developmental defects resulting from ΔflbA and dominant activating fadA mutations, which both cause constitutive induction of the mycelial proliferation pathway. However, dsgA1 does not suppress the negative influence of these mutations on production of the aflatoxin precursor, sterigmatocystin, indicating that dsgA1 is specific for asexual development. Taken together, our studies define dsgA as a novel component of the asexual sporulation pathway.

scholarly journals Structural and functional characterization of a highly secreted α- l -arabinofuranosidase (GH62) from Aspergillus nidulans grown on sugarcane bagasse

2017 ◽  
Vol 1865 (12) ◽  
pp. 1758-1769 ◽  
Author(s):  
Fabiano Jares Contesini ◽  
Marcelo Vizoná Liberato ◽  
Marcelo Ventura Rubio ◽  
Felipe Calzado ◽  
Mariane Paludetti Zubieta ◽  
...  
Keyword(s):  
Aspergillus Nidulans ◽  
Sugarcane Bagasse ◽  
Functional Characterization
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