Xylitol production by Petromyces albertensis grown on medium containing D-xylose

1991 ◽  
Vol 37 (1) ◽  
pp. 14-18 ◽  
Author(s):  
Jagroop S. Dahiya
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Key Words ◽  
Yeast Extract ◽  
High Performance ◽  
Ammonium Acetate ◽  
Xylitol Production ◽  
Maximum Production ◽  
Initial Ph ◽  
Fermentative Products

Petromyces albertensis produced xylitol and D-xylulose when cultivated on a medium containing D-xylose. These fermentative products were identified by high-performance liquid chromatography. A large amount of xylitol was obtained from a D-xylose medium containing ammonium acetate and yeast extract at an initial pH of 7.0. Maximum production of xylitol and of the enzymes concerned with its production was observed after 10 days of cultivation. A D-xylose (100 g/L) medium supplemented with 1% (v/v) methanol gave the highest yields of xylitol (39.8 g/L) and D-xylulose (2.8 g/L). Key words: Petromyces albertensis, D-xylulose, xylitol.

scholarly journals Attempted fractionation of LB Lennox medium via reversed phase high performance liquid chromatography

2020 ◽  
Author(s):  
Wenfa Ng
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Growth Medium ◽  
Yeast Extract ◽  
High Performance ◽  
Chemical Engineering ◽  
Reversed Phase ◽  
Model Systems ◽  
Water Mixture ◽  
Rp Hplc

AbstractMass balance analysis is a highly useful tool for chemical engineering analysis of biological processes. Specifically, composition and amounts of inputs to a cell could be correlated with measurement of metabolic byproducts and outputs for inferring metabolic fluxes flowing through specific cellular metabolic pathways. However, the composition of many common microbiological growth medium remains ill-defined with batch-to-batch variation. Thus, a need exists for developing methods for effective fractionation and separation of common growth medium. Using 5 g/L yeast extract, 10 g/L tryptone, and LB Lennox medium as model systems, this work attempted the fractionation of the three complex growth mixtures using C-18 reversed phase high performance liquid chromatography (RP-HPLC). Results revealed no effective fractionation of the three mixtures. More importantly, experiment results indicated that appropriate choice of detection wavelength for visualizing the chromatogram made a huge difference to understanding the effectiveness of fractionation achieved. Specifically, in the case of yeast extract, tryptone and LB Lennox medium, 194 nm may be a more appropriate detection wavelength compared to 280 nm. Collectively, C-18 RP-HPLC was not effective in separating 5 g/L yeast extract, 10 g/L tryptone and LB Lennox medium with hydrophilic mobile phases (ethanol/water mixture).Graphical abstractShort description: Visualization of the reversed phase HPLC chromatogram at 194 nm revealed broad peaks and lack of distinct peaks indicative of fractionation of LB Lennox medium by the chromatography method. More importantly, complex ensemble of different components in the growth medium present a significant separation challenge that precludes separation or fractionation by modern liquid chromatography methods. Overall, combinatorial use of both hydrophobic and hydrophilic mobile phase with a C-18 reversed phase column could reveal the presence of a couple of main fractions in a mixture otherwise unable to be separated into distinct components.

scholarly journals Phenobarbital Analysis in Biological Matrix (Blood) by High Performance Liquid Chromatography (HPLC)

2013 ◽  
Vol 20 ◽  
pp. 31-40
Author(s):  
Ouakouak Hamza ◽  
Ben Mohamed Moktar ◽  
Ben Chohra Mostafa ◽  
Abdelhamid Zeghdaoui
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Ammonium Acetate ◽  
Analytical Techniques ◽  
Hplc Method ◽  
Diode Array ◽  
Blood Components ◽  
Ammonium Acetate Buffer

In this work, we have tried to contribute to the analysis of phenobarbital in the blood. To do this, we used different analytical techniques such as liquid chromatography (HPLC). The results obtained in the course of our work, we can conclude that: The HPLC method was separate phenobarbital endogenous blood components, and optimizing the conditions of chromatographic separation as the composition of the mobile phase consisting of 20% acetonitrile + 20% methanol + 60% ammonium acetate buffer. The extraction with diethyl ether to pH = 4; The chromatographic column, microbondapack ZORBAX C18. A system of diode-array UV detection at 254 nm.

Analysis of Bifenthrin and its Enantiomer Using High Performance Liquid Chromatography

2014 ◽  
Vol 675-677 ◽  
pp. 275-279 ◽  
Author(s):  
Su Su Fan ◽  
Jian Shi ◽  
Ling Zhou ◽  
Yu Wen Hang
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Chiral Stationary Phase ◽  
Ammonium Acetate ◽  
Hplc Method ◽  
Column Temperature ◽  
Polysaccharide Derivatives ◽  
Separation Results

Using the high performance liquid chromatography (HPLC) method, bifenthrin isomers can be split at a polysaccharide derivatives chiral stationary phase column, and two well distinguished peaks of bifenthrin isomers are obtained. The effects of mobile phase ratios, temperatures, and detection wavelengths on the separation results are discussed. The optimal chromatographic conditions are as follows: the mobile phase ratio is methanol: ammonium acetate salts = 80:20, the column temperature is 35°C, and the wavelength is set as 220 nm. Under the optimal conditions, the resolution of bifenthrin enantiomer can be as large as 3.0.

scholarly journals Morphology Character and Andrographolide Quantifications on Sambiloto  ( Andrographis paniculata (Burm.F.) Nees) Karakter Morfologi dan Kuantifikasi Andrografolid Pada Sambiloto ( Andrographis paniculata (Burm.F.) Nees )

BioScience ◽  
2020 ◽  
Vol 4 (1) ◽  
pp. 109
Author(s):  
Retno Prihatini ◽  
Auzar Syarif ◽  
Amri Bakhtiar
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Key Words ◽  
High Performance ◽  
Economic Value ◽  
Dry Weight ◽  
Andrographis Paniculata ◽  
Flowering Rate ◽  
Length Width ◽  
Leaf Dry Weight

ABSTRACTThe Morphology character and andrographolide quantification on Sambiloto (A. Paniculata) that growth in Sawah Dangka Jorong Gaduik, Tilatang Kamang, Agam (S 00o16159.311 E100o23110.311)  have been studied.  The Morphology character  of  A. Paniculata on generative fase  have been done in the field and Laboratorium of Biology Department, Andalas University. Quantification of  andrographolide constituen were analysed by using High Performance Liquid Chromatography (HPLC) in Chemical of Nature Resources Laboratorium, Pharmacy Faculty, Andalas University. The result showed that Morphology character of  Sambiloto ( A. Paniculata ) include parameters  ie. range of height plant ( 27-45 cm) ; range of nodus numerous ( 10-14 ); range of leaf-7 size (length; width)  ( 3.8-4.1 ; 0.8-1.1) cm; range of branch numerous ( 8-10), leaf dry weight rate  (2.482 g ), stem dry weight rate  ( 5.882 g ), and day old flowering rate  ( 110 day ). The Result of quantification secondary metabolite with high economic value, namely andrographolide showed respectively with level  2.208% (vegetative) and  2.780 % (generative).  Key Words : Andrographis paniculata, morpology chracter, andrographolide quantification  ABSTRAKTelah dilakukan studi tentang Karakter morfologi dan kuantifikasi senyawa andrografolide pada tanaman Sambiloto (A. Paniculata) yang hidup di Sawah Dangka Jorong Gaduik, Tilatang Kamang, Agam (S 00o16159.311 E100o23110.311). Karakter morfologi   A. Paniculata pada fase generative telah dilakukan di lapangan dan di  Laboratorium di Jurusan Biologi Universitas Andalas. Kuantifikasi senyawa andrografolid dianalisa dengan menggunakan High Performance Liquid Chromatography (HPLC) di Laboratorium Kimia Bahan Alam, Fakultas Farmasi Universitas Andalas.  Hasil menunjukkan bahwa karakter morfologi Sambiloto    ( A. Paniculata ) meliputi parameter  yaitu kisaran tinggi tanaman ( 27-45 cm) ; kisaran jumlah nodus ( 10-14 ); kisaran ukuran daunke-7  (panjang; lebar)  ( 3,8-4,1 ; 0,8-1,1) cm; kisaran jumlah cabang ( 8-10), rata-rata berat kering daun  (2,482 g ), rata-rata berat kering batang ( 5,882 g ), and rata-rata umur berbunga  ( 110 day ).  Hasil kuantifikasi metabolit sekunder dengan nilai ekonomi tinggi, yang dikenal sebagai senyawa andrografolid menunjukkan level   2,208%  (vegetatif) dan 2,780 % (generatif). Kata kunci: Andrographis paniculata, karakter morfologi, kuantifikasi andrografolid. 

High Performance Liquid Chromatography Using Ultrasonic Extraction for Benzoic Acid Analysis in Curry Paste Samples

2019 ◽  
Vol 886 ◽  
pp. 40-45
Author(s):  
Nararat Thongsrinoon ◽  
Netnapha Phiwdee ◽  
Yanada Duangsa ◽  
Khaengkhae Muensub ◽  
Vichayaporn Duang-Iad
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Benzoic Acid ◽  
Mobile Phase ◽  
High Performance ◽  
Ammonium Acetate ◽  
Ultrasonic Extraction ◽  
Optimum Conditions ◽  
Ammonium Acetate Buffer ◽  
Uv Visible

Benzoic acid analysis in curry paste samples were carried out by using high performance liquid chromatography using ultrasonic extraction. Methanol-0.05 M ammonium acetate buffer pH 4.40 in the ratio of 55:45 (%v/v) at a flow rate of 1.00 mL/min was used as the mobile phase and benzoic acid detection was performed at 226 nm using an UV-Visible detector. Under the optimum conditions, linearity of spiked samples ranged from 50 to 3,000 mg/kg. Matrix matched calibrations had determined that benzoic acid contents in southern sour, red, and green curry paste samples were 67.59, 78.62 and 72.33 mg/kg, respectively. Recoveries were obtained from 89.34 to 101.70%, 83.37 to 130.30% and 92.75 to 113.56% with R.S.D. ranged from 2.71 to 6.53%, 4.02 to 11.58% and 5.81 to 6.35%, for southern sour, red, and green curry paste samples, respectively.

scholarly journals High-performance liquid chromatography of type-III heptocarboxylic porphyrinogen isomers

1987 ◽  
Vol 247 (1) ◽  
pp. 229-232 ◽  
Author(s):  
C K Lim ◽  
F Li ◽  
T J Peters
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Ammonium Acetate ◽  
Reversed Phase ◽  
Buffer Concentration ◽  
Organic Modifier ◽  
Type Iii ◽  
Optimum Separation

A reversed-phase h.p.l.c. system is described for the separation of the four type-III heptacarboxylic porphyrinogen isomers. The effects of buffer concentration, pH and type and proportion of organic modifier in the mobile phase on retention and resolution of isomers were studied. Optimum separation on an ODS-Hypersil column was by elution with a ternary mobile phase of acetonitrile, methanol and 1 M-ammonium acetate, pH 5.16 (7:3:90, by vol.). Isomer identification was based on a comparison of their retention times with those of authentic standards, and was further confirmed by h.p.l.c. analysis of the characteristic mixture of three pentacarboxylic porphyrins formed after partial decarboxylation of individual isomers in 0.3 M-HCl at 160 degrees C.

scholarly journals Glucosinolate composition of broccoli (Brassica oleracea var. italica) grown under various boron treatments at three Ontario sites

1993 ◽  
Vol 73 (3) ◽  
pp. 885-888 ◽  
Author(s):  
B. J. Shelp ◽  
L. Liu ◽  
D. McLellan
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Key Words ◽  
Brassica Oleracea ◽  
High Performance ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Glucosinolate Concentration

The relative glucosinolate composition of the florets from field-grown broccoli, determined using high-performance liquid chromatography and liquid chromatography–mass spectrometry, varied slightly with growing site and B fertilization, but was cultivar-dependent. Differences among growing sites, in total glucosinolate concentration, were greater than differences between the two cultivars used in the study. Key words: Brassica, broccoli, glucosinolate, nutrition

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