Identification of a denitrifying gliding bacterium, isolated from soil and able to reduce nitrous oxide in the presence of sulfide and acetylene, as Flexibacter canadensis

1990 ◽  
Vol 36 (11) ◽  
pp. 765-770 ◽  
Author(s):  
Alison M. Jones ◽  
Anne M. Adkins ◽  
Roger Knowles ◽  
Gina R. Rayat
Keyword(s):  
Nitrous Oxide ◽  
Key Words ◽  
Dna Hybridization ◽  
Inhibition Assay ◽  
Acetylene Inhibition ◽  
Biochemical Tests ◽  
Gliding Bacteria ◽  
Soil Isolate ◽  
Gliding Bacterium ◽  
Physiological And Biochemical

We have reexamined the properties of a gliding bacterium, Is-11, which was previously isolated from soil because of its ability to denitrify and to reduce nitrous oxide in the presence of sulfide and normally inhibitory concentrations of acetylene. Occurrence of such an organism may have important implications for the use of the acetylene inhibition assay for measuring denitrification rates in reduced, sulfidic environments. Although originally tentatively identified as a Cytophaga sp., extensive morphological, physiological, and biochemical tests as well as G+C analysis and DNA hybridization studies now indicate that the soil isolate Is-11 is a strain of Flexibacter canadensis. Key words: gliding bacteria, Flexibacter canadensis, denitrification, acetylene, sulfide, nitrous oxide.

Denitrifïcation in Flexibacter canadensis

1990 ◽  
Vol 36 (6) ◽  
pp. 430-434 ◽  
Author(s):  
Alison M. Jones ◽  
Roger Knowles
Keyword(s):  
Nitrous Oxide ◽  
Sodium Sulfide ◽  
Electron Acceptors ◽  
Inhibition Assay ◽  
Oxide Reduction ◽  
Acetylene Inhibition ◽  
Gram Negative ◽  
Nitrous Oxide Reduction ◽  
Pure Cultures ◽  
Gliding Bacterium

Denitrifïcation was studied in pure cultures of Flexibacter canadensis (ATCC 29591), a Gram-negative gliding bacterium found in soil. Flexibacter canadensis was capable of using nitrate, nitrite, and nitrous oxide as terminal electron acceptors for growth. Sodium sulfide (200 μM) inhibited all of the nitrogen oxide reductases, but only temporarily. Acetylene (4 kPa) inhibited nitrous oxide reduction but did not affect the reduction of either nitrate or nitrite. However, sulfide (100 and 200 μM) alleviated the acetylene block and permitted reduction of nitrous oxide in the presence of 4 kPa acetylene. These data may have important implications regarding the use of the acetylene inhibition assay for measuring denitrifïcation rates in highly anaerobic, sulfidic environments. Key words: Flexibacter canadensis, denitrification, N2O reductase, sulfide, acetylene.

scholarly journals Citricoccus parietis sp. nov., isolated from a mould-colonized wall and emended description of Citricoccus alkalitolerans Li et al. 2005

2010 ◽  
Vol 60 (2) ◽  
pp. 271-274 ◽  
Author(s):  
Jenny Schäfer ◽  
Karin Martin ◽  
Peter Kämpfer
Keyword(s):  
Dna Hybridization ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Polar Lipid Profile ◽  
Phenotypic Differentiation ◽  
Emended Description ◽  
Diamino Acid ◽  
Physiological And Biochemical ◽  
Sequence Similarities

A Gram-positive, coccoid-shaped organism (strain 02-Je-010T), forming yellow-pigmented colonies was isolated from the wall of an indoor environment. On the basis of 16S rRNA gene sequence similarity studies, it was shown that strain 02-Je-010T belongs to the genus Citricoccus with sequence similarities of 98.9 % to Citricoccus alkalitolerans DSM 15665T and 98.6 % to Citricoccus muralis DSM 14442T. Cell-wall sugars were mannose and glucose. The diagnostic diamino acid of the peptidoglycan was lysine. The major menaquinones detected were MK-9(H2) and MK-8(H2). The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and moderate amounts of two unknown phospholipids and two unknown glycolipids. The fatty acid profile comprised major amounts of anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0. All these data supported the affiliation of strain 02-Je-010T to the genus Citricoccus. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 02-Je-010T from the two recognized Citricoccus species. For these reasons, strain 02-Je-010T represents a novel species, for which the name Citricoccus parietis sp. nov. is proposed, with the type strain 02-Je-010T (=CCUG 57388T=CCM 7609T).

scholarly journals Halorubrum tibetense sp. nov., a novel haloalkaliphilic archaeon from Lake Zabuye in Tibet, China

2004 ◽  
Vol 54 (4) ◽  
pp. 1213-1216 ◽  
Author(s):  
Huapeng Fan ◽  
Yanfen Xue ◽  
Yanhe Ma ◽  
Antonio Ventosa ◽  
William D. Grant
Keyword(s):  
Dna Hybridization ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
The Tibetan Plateau ◽  
Phylogenetic Distance ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Gene Sequence Analysis ◽  
Derivatives Of ◽  
Physiological And Biochemical

A novel haloalkaliphilic archaeon, strain 8W8T, was isolated from Lake Zabuye, on the Tibetan Plateau, China. On the basis of 16S rRNA gene sequence analysis, strain 8W8T was shown to belong to the genus Halorubrum and was related to Halorubrum vacuolatum (96·7 % sequence similarity), Halorubrum saccharovorum (96·0 %), Halorubrum lacusprofundi (95·4 %) and Halorubrum sodomense (95·3 %). The phylogenetic distance from any species within the other genera of Halobacteriales was lower than 90 %. The major polar lipids of strain 8W8T were C20C20 and C20C25 derivatives of phosphatidylglycerol phosphate and phosphatidylglycerol phosphate methyl ester. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 8W8T from the eight Halorubrum species with validly published names. Therefore, strain 8W8T represents a novel species, for which the name Halorubrum tibetense sp. nov. is proposed, with the type strain 8W8T (=AS 1.3239T=JCM 11889T).

scholarly journals Methylocystis rosea sp. nov., a novel methanotrophic bacterium from Arctic wetland soil, Svalbard, Norway (78° N)

2006 ◽  
Vol 56 (3) ◽  
pp. 541-547 ◽  
Author(s):  
Ingvild Wartiainen ◽  
Anne Grethe Hestnes ◽  
Ian R. McDonald ◽  
Mette M. Svenning
Keyword(s):  
Dna Hybridization ◽  
Sequence Similarity ◽  
Major Fatty Acid ◽  
Wetland Soil ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Phenotypic Data ◽  
Phenotypic Differentiation ◽  
Type Strains ◽  
Physiological And Biochemical

A Gram-negative, rod-shaped, non-motile, non-spore-forming, pink-pigmented bacterium, SV97T, was isolated from a wetland soil near Ny-Ålesund, Svalbard Islands, Norway (78° N). On the basis of 16S rRNA gene sequence similarity, strain SV97T was shown to belong to the Alphaproteobacteria and was highly related to a number of non-characterized Methylocystis strains with GenBank accession nos AJ458507 and AJ458502 (100 %) and AF177299, AJ458510, AJ458467, AJ458471, AJ431384, AJ458475, AJ458484, AJ458501 and AJ458466 (99 %). The most closely related type strains were Methylocystis parvus OBBPT (97·2 %) and Methylocystis echinoides IMET 10491T (97 %). The closest related recognized species within the genus Methylosinus was Methylosinus sporium NCIMB 11126T (96·0 % similarity). Chemotaxonomic and phenotypic data (C18 : 1 ω8 as the major fatty acid, non-motile, no rosette formation) supported the affiliation of strain SV97T to the genus Methylocystis. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain SV97T from the two recognized Methylocystis species. Strain SV97T therefore represents a novel species, for which the name Methylocystis rosea sp. nov. is proposed, with the type strain SV97T (=DSM 17261T=ATCC BAA-1196T).

scholarly journals Streptomyces graminifolii sp. nov., isolated from bamboo (Sasa borealis) litter

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2517-2521 ◽  
Author(s):  
Hyo-Jin Lee ◽  
Kyung-Sook Whang
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Gene Sequence ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Content Type ◽  
Link Type ◽  
Physiological And Biochemical ◽  
The 16S Rrna Gene

The taxonomic position of strain JL-22T, isolated from litter of a bamboo (Sasa borealis) forest, was determined using a polyphasic approach. The organism had phenotypic and morphological properties consistent with it being a member of the genus Streptomyces . Phylogenetic analysis of the 16S rRNA gene sequence showed that strain JL-22T was closely related to Streptomyces prunicolor NRRL B-12281T (99.2 %), Streptomyces galilaeus JCM 4757T (99.0 %) and Streptomyces chartreusis NBRC 12753T (99.0 %). However, the results of DNA–DNA hybridization and physiological and biochemical tests showed that strain JL-22T could be differentiated from its closest phylogenetic relatives both genotypically and phenotypically. Based on phenotypic and genotypic data, strain JL-22T represents a novel species of the genus Streptomyces , for which the name Streptomyces graminifolii sp. nov. is proposed. The type strain is JL-22T ( = KACC 17180T = NBRC 109806T).

Denitrification by Cytophaga johnsonae strains and by a gliding bacterium able to reduce nitrous oxide in the presence of acetylene and sulfide

1986 ◽  
Vol 32 (5) ◽  
pp. 421-424 ◽  
Author(s):  
Anne M. Adkins ◽  
Roger Knowles
Keyword(s):  
Nitrous Oxide ◽  
Nitrogen Oxide ◽  
Electron Acceptors ◽  
Gliding Bacteria ◽  
Gliding Bacterium

A gliding bacterium (Is-11), isolated for its ability to reduce nitrous oxide (N2O) in the presence of acetylene (C2H2) and sulfide, was able to use nitrate (NO3−), nitrite (NO2−), and N2O as terminal electron acceptors for growth. Similarly, of five strains of Cytophaga johnsonae examined, two were capable of complete denitrification. Two strains were unable to reduce nitrate and N2O, respectively, and the remaining strain lacked both nitrate and N2O reductases. However, in this strain the N2O reductase was induced by the presence of nitrite, but not nitrate. Acetylene inhibited N2O reduction but did not affect the reduction of nitrite or nitrate in all of the gliding bacteria tested. Sulfide temporarily inhibited all of the nitrogen oxide reductases. It did not relieve the C2H2 inhibition of N2O reduction in the C. johnsonae strains when tested under the same conditions under which C2H2 inhibition is relieved in Is-11.

scholarly journals Devosia limi sp. nov., isolated from a nitrifying inoculum

2005 ◽  
Vol 55 (5) ◽  
pp. 1997-2000 ◽  
Author(s):  
Bram Vanparys ◽  
Kim Heylen ◽  
Liesbeth Lebbe ◽  
Paul De Vos
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Sds Page ◽  
Spore Forming Bacteria ◽  
Physiological And Biochemical

A Gram-negative, rod-shaped, non-spore-forming bacteria was isolated from a nitrifying inoculum. On the basis of 16S rRNA gene sequence similarity, this strain, designated LMG 22951T, was shown to belong to the ‘Alphaproteobacteria’ and to be related to Devosia neptuniae (97·4 %) and Devosia riboflavina (97·0 %). The results of DNA–DNA hybridization, analysis of fatty acid composition, SDS-PAGE, physiological and biochemical tests allowed genotypic and phenotypic differentiation of LMG 22951T from the two recognized Devosia species. LMG 22951T therefore represents a novel species within this genus, for which the name Devosia limi is proposed. The type strain is LMG 22951T (=DSM 17137T).

scholarly journals Thermomonas koreensis sp. nov., a mesophilic bacterium isolated from a ginseng field

2006 ◽  
Vol 56 (7) ◽  
pp. 1615-1619 ◽  
Author(s):  
Myung Kyum Kim ◽  
Wan-Taek Im ◽  
Jun-Gyo In ◽  
Sung-Hoon Kim ◽  
Deok-Chun Yang
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Distinct Species ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Gene Sequence Analysis ◽  
Mesophilic Bacterium ◽  
Bacterium Strain ◽  
Physiological And Biochemical

A Gram-negative, non-spore-forming, rod-shaped, motile bacterium, strain Ko06T, was isolated from soil from a ginseng field in South Korea and was characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain Ko06T belongs to the Gammaproteobacteria, and the highest levels of sequence similarity were with Thermomonas brevis LMG 21746T (98.4 %), Thermomonas fusca LMG 21737T (97.7 %), Thermomonas haemolytica A50-7-3T (96.5 %) and Thermomonas hydrothermalis SGM-6T (95.8 %). Chemotaxonomic data revealed that strain Ko06T possesses ubiquinone Q-8 and that the predominant fatty acids are C15 : 0 iso, C11 : 0 iso and C11 : 0 iso 3-OH, all of which corroborated assignment of the strain to the genus Thermomonas. The results of DNA–DNA hybridization and physiological and biochemical tests clearly demonstrated that strain Ko06T represents a distinct species. On the basis of these data, strain Ko06T (=KCTC 12540T=NBRC 101155T) should be classified as the type strain of a novel Thermomonas species, for which the name Thermomonas koreensis sp. nov. is proposed.

scholarly journals Stenotrophomonas ginsengisoli sp. nov., isolated from a ginseng field

2010 ◽  
Vol 60 (7) ◽  
pp. 1522-1526 ◽  
Author(s):  
Ho-Bin Kim ◽  
Sathiyaraj Srinivasan ◽  
Gayathri Sathiyaraj ◽  
Lin-Hu Quan ◽  
Se-Hwa Kim ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Biochemical Tests ◽  
Gene Sequence Analysis ◽  
Stenotrophomonas Nitritireducens ◽  
Physiological And Biochemical

A Gram-negative, non-spore-forming, rod-shaped bacterium, designated strain DCY01T, was isolated from soil from a ginseng field in South Korea and was characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain DCY01T belonged to the Gammaproteobacteria and was most closely related to Stenotrophomonas koreensis KCTC 12211T (98.4 % similarity), Stenotrophomonas humi R-32729T (97.2 %), Stenotrophomonas terrae R-32768 (97.1 %), Stenotrophomonas maltophilia DSM 50170T (96.9 %) and Stenotrophomonas nitritireducens DSM 12575T (96.8 %). Chemotaxonomic analyses revealed that strain DCY01T possessed a quinone system with Q-8 as the predominant compound, and iso-C15 : 0 (28.2 %), C16 : 0 10-methyl (13.2 %), iso-C15 : 1 F (10.8 %) and C15 : 0 (7.5 %) as major fatty acids, corroborating assignment of strain DCY01T to the genus Stenotrophomonas. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The results of DNA–DNA hybridization and physiological and biochemical tests clearly demonstrated that strain DCY01T represents a species distinct from recognized Stenotrophomonas species. Based on these data, DCY01T (=KCTC 12539T=NBRC 101154T) should be classified as the type strain of a novel species of the genus Stenotrophomonas, for which the name Stenotrophomonas ginsengisoli sp. nov. is proposed.

scholarly journals Herminiimonas saxobsidens sp. nov., isolated from a lichen-colonized rock

2007 ◽  
Vol 57 (11) ◽  
pp. 2618-2622 ◽  
Author(s):  
Elke Lang ◽  
Jolantha Swiderski ◽  
Erko Stackebrandt ◽  
P. Schumann ◽  
Cathrin Spröer ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rock Surface ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Bacterium Strain ◽  
Physiological And Biochemical

A Gram-negative, rod-shaped, non-spore-forming bacterium (strain NS11T) was isolated from a lichen-colonized rock surface. On the basis of 16S rRNA gene sequence similarity, strain NS11T was shown to belong to the Betaproteobacteria, and was most closely related to Herminiimonas arsenicoxydans ULPAs1T (98.8 %), Herminiimonas aquatilis CCUG 36956T (98.0 %) and Herminiimonas fonticola S-94T (98.0 %). Major whole-cell fatty acids were C16 : 0, C17 : 0 cyclo and C16 : 1 ω7c. Strain NS11T also contained high proportions of C10 : 0 3-OH and C18 : 1 ω7c. This pattern is typical for members of the genus Herminiimonas. The results of DNA–DNA hybridization experiments and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain NS11T from the three recognized Herminiimonas species. It is therefore concluded that strain NS11T represents a novel species of the genus Herminiimonas, for which the name Herminiimonas saxobsidens sp. nov. is proposed. The type strain is NS11T (=DSM 18748T=CCM 7436T).

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