Biotransformation of dibenzothiophene to dibenzothiophene sulfone by Pseudomonas putida

1989 ◽  
Vol 35 (5) ◽  
pp. 603-605 ◽  
Author(s):  
MeLanie R. Mormile ◽  
Ronald M. Atlas
Keyword(s):  
Pseudomonas Putida ◽  
Microbial Degradation ◽  
Sulfur Metabolism ◽  
Crystalline Compound ◽  
Dibenzothiophene Sulfone

A strain of Pseudomonas putida was isolated that transforms dibenzothiophene (DBT) to DBT sulfone (DBT-5-dioxide) via DBT-5-oxide. It also degrades DBT to 3-hydroxy-2-formyl benzothiophene via an alternate and previously described pathway, and to an unidentified red crystalline compound. Neither DBT sulfone nor 3-hydroxy-2-formyl benzothiophene are further degraded by this organism.Key words: dibenzothiophene, microbial degradation, Pseudomonas, dibenzothiophene sulfone, sulfur metabolism.

Microbial degradation of the morphine alkaloids: identification of morphinone as an intermediate in the metabolism of morphine by Pseudomonas putida M10

1990 ◽  
Vol 154 (5) ◽  
pp. 465-470 ◽  
Author(s):  
Neil C. Bruce ◽  
Clare J. Wilmot ◽  
Keith N. Jordan ◽  
Anna E. Trebilcock ◽  
Lauren D. Gray Stephens ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Microbial Degradation ◽  
Morphine Alkaloids

scholarly journals Pathways of Assimilative Sulfur Metabolism inPseudomonas putida

1999 ◽  
Vol 181 (18) ◽  
pp. 5833-5837 ◽  
Author(s):  
Paul Vermeij ◽  
Michael A. Kertesz
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pseudomonas Putida ◽  
Sulfur Metabolism ◽  
Sulfur Source ◽  
Methionine Biosynthesis ◽  
Cysteine Synthase ◽  
Transsulfuration Pathway ◽  
Key Enzyme ◽  
Low Levels

ABSTRACT Cysteine and methionine biosynthesis was studied inPseudomonas putida S-313 and Pseudomonas aeruginosa PAO1. Both these organisms used direct sulfhydrylation of O-succinylhomoserine for the synthesis of methionine but also contained substantial levels of O-acetylserine sulfhydrylase (cysteine synthase) activity. The enzymes of the transsulfuration pathway (cystathionine γ-synthase and cystathionine β-lyase) were expressed at low levels in both pseudomonads but were strongly upregulated during growth with cysteine as the sole sulfur source. In P. aeruginosa, the reverse transsulfuration pathway between homocysteine and cysteine, with cystathionine as the intermediate, allows P. aeruginosa to grow rapidly with methionine as the sole sulfur source. P. putida S-313 also grew well with methionine as the sulfur source, but no cystathionine γ-lyase, the key enzyme of the reverse transsulfuration pathway, was found in this species. In the absence of the reverse transsulfuration pathway, P. putida desulfurized methionine by the conversion of methionine to methanethiol, catalyzed by methionine γ-lyase, which was upregulated under these conditions. A transposon mutant of P. putida that was defective in the alkanesulfonatase locus (ssuD) was unable to grow with either methanesulfonate or methionine as the sulfur source. We therefore propose that in P. putida methionine is converted to methanethiol and then oxidized to methanesulfonate. The sulfonate is then desulfonated by alkanesulfonatase to release sulfite for reassimilation into cysteine.

scholarly journals Distribution of Camphor Monooxygenase Genes in Soil Bacteria

2015 ◽  
Vol 10 (2) ◽  
Author(s):  
N. Ngadiman ◽  
Hikaru Suenaga ◽  
Masatoshi Goto ◽  
Kensuke Furukawa
Keyword(s):  
Pseudomonas Putida ◽  
Microbial Degradation ◽  
Genomic Dna ◽  
Soil Bacteria ◽  
Southern Analysis ◽  
Bacterial Strains ◽  
Gene Distribution

In microbial degradation of camphor, the first step is oxidation by multiunit enzyme, camphormonooxygenase, encoded by cam genes (camA,B,C). Seven camphor-utilizing bacterial strains have been isolatedfrom soil at various locations. CamA,B,C genes of Pseudomonas putida strain PpG1 and strain GF2001 were used asprobes to explore their abundance in the camphor-utilizing bacteria. Southern analysis revealed that all of thecam genes of GF2001 could hybridize well to the SpeI-digested genomic DNA of strains tested, whereas PpG1 camgenes were not. This result suggested that the GF2001 type cam genes are widely distributed among the camphorutilizingstrains in the environment. Thus strain GF2001 and seven newly isolated strains share a commonevolutionary origin.Key words: Camphor monooxygenase genes, gene distribution, sail bacteria.

scholarly journals Microbial degradation of the morphine alkaloids. Purification and characterization of morphine dehydrogenase from Pseudomonas putida M10

1991 ◽  
Vol 274 (3) ◽  
pp. 875-880 ◽  
Author(s):  
N C Bruce ◽  
C J Wilmot ◽  
K N Jordan ◽  
L D G Stephens ◽  
C R Lowe
Keyword(s):  
Affinity Chromatography ◽  
Pseudomonas Putida ◽  
Hydroxy Group ◽  
Microbial Degradation ◽  
Purification Procedure ◽  
Metal Centre ◽  
Purification And Characterization ◽  
Ph Optimum ◽  
Morphine Alkaloids

The NADP(+)-dependent morphine dehydrogenase that catalyses the oxidation of morphine to morphinone was detected in glucose-grown cells of Pseudomonas putida M10. A rapid and reliable purification procedure involving two consecutive affinity chromatography steps on immobilized dyes was developed for purifying the enzyme 1216-fold to electrophoretic homogeneity from P. putida M10. Morphine dehydrogenase was found to be a monomer of Mr 32,000 and highly specific with regard to substrates, oxidizing only the C-6 hydroxy group of morphine and codeine. The pH optimum of morphine dehydrogenase was 9.5, and at pH 6.5 in the presence of NADPH the enzyme catalyses the reduction of codeinone to codeine. The Km values for morphine and codeine were 0.46 mM and 0.044 mM respectively. The enzyme was inhibited by thiol-blocking reagents and the metal-complexing reagents 1,10-phenanthroline and 2,2′-dipyridyl, suggesting that a metal centre may be necessary for activity of the enzyme.

Enzimaktivitások és a fluoreszkáló pszeudomonasz csíraszámok változása a fehér lóhere (Trifolium repens L.) rizoszférájában sókezelés (NaCl) hatására

2004 ◽  
Vol 53 (3-4) ◽  
pp. 367-376 ◽  
Author(s):  
A. A. Khalif ◽  
H. Abdorhim ◽  
Hosam E. H. T. Bayoumi ◽  
Anna Füzy ◽  
Mihály Kecskés
Keyword(s):  
Pseudomonas Putida ◽  
Trifolium Repens ◽  
Trifolium Repens L

Üvegházi körülmények között savanyú barna erdotalajban nevelt fehér here (Trifolium repens L.) növények rizoszférájának sókezelés hatására bekövetkezo változását ellenoriztük. Megvizsgáltuk a különbözo sókoncentrációknak (0, 0,2, 0,4, 0,6 és 0,8 tömeg %) a baktériumnépesség összetételére és a különbözo talajenzimek aktivitására gyakorolt hatását.  Megállapítottuk, hogy a talaj sótartalma közvetlenül befolyásolta a rizoszférában található fluoreszkáló pszeudomonaszok csíraszámát. A legsurubb populáció a 0,2% NaCl-ot tartalmazó talajban volt mérheto, ahol a fluoreszkáló pszeudomonaszok között a Pseudomonas putida és a P. fluorescens fordultak elo a legnagyobb számban. A pszeudomonaszok ily módon jól tolerálják a talaj magas NaCl-tartalmát, és gyökérkolonizáló tevékenységet képesek kifejteni a magas NaCl-tartalmú talajban is. A sókoncentráció növelésével kezdetben (a 0,2-0,4%-os tartományban) jelentosen növekedett a dehidrogenáz, kataláz, és ureáz enzimek aktivitása. A proteáz enzimek aktivitásmaximuma a 0,1-0,2% NaCl-koncentráció tartományba esett. A 0,4%-nál magasabb koncentrációkban a kontrollhoz hasonló mértékure csökkent mind a négy enzim aktivitása, és a baktériumok száma is. A foszfatáz- és a b-glükozidáz-tevékenység viszont a NaCl-dózis növelése következtében a koncentrációval arányosan, jelentosen csökkent a kontrollhoz viszonyítva.  Feltételezésünk szerint az enzimaktivitások változását is a sókezelés hatására bekövetkezo mikrobióta összetételének megváltozása okozta.

Exemplar Abstract for Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Pseudomonas Putida
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