The role of an extracellular polysaccharide produced by the marine Pseudomonas sp. S9 in cellular detachment during starvation

1989 ◽  
Vol 35 (2) ◽  
pp. 309-312 ◽  
Author(s):  
Michael Wrangstadh ◽  
Patricia L. Conway ◽  
Staffan Kjelleberg
Keyword(s):  
Cell Surface ◽  
Immunofluorescence Microscopy ◽  
Cell Surface Hydrophobicity ◽  
Hydrophobic Surface ◽  
Extracellular Polysaccharide ◽  
Surface Hydrophobicity ◽  
Bulk Phase ◽  
Bacterial Cells ◽  
Pseudomonas Sp

An exopolysaccharide polymer is produced by the marine Pseudomonas sp. S9 in response to complete energy and nutrient starvation. The presence of this polysaccharide on the cell surface and its subsequent release have been shown to be associated with both adhesion and detachment of the bacterial cells. Detachment from a hydrophobic surface was correlated to the presence of the exopolysaccharide on detached S9 cells. The exopolysaccharide was detected, using immunofluorescence microscopy, on surface-bound cells after only 15 min of exogenous energy and nutrient deprivation. This technique did not reveal any significant amounts of exopolysaccharide on starving bulk phase cells prior to 3 h of starvation. Cells that detached after 5.5 h of starvation had low cell surface hydrophobicity values and increased amounts of cell-bound exopolysaccharide. In contrast, cells that became detached during the first 5.5 h of starvation showed increasing hydrophobicity values during prolonged bulk phase starvation.

Role of lactobacillus cell surface hydrophobicity as probed by AFM in adhesion to surfaces at low and high ionic strength

2005 ◽  
Vol 41 (1) ◽  
pp. 33-41 ◽  
Author(s):  
Virginia Vadillo-Rodríguez ◽  
Henk J. Busscher ◽  
Henny C. van der Mei ◽  
Joop de Vries ◽  
Willem Norde
Keyword(s):  
Ionic Strength ◽  
Cell Surface ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
High Ionic Strength

scholarly journals Factors associated with adherence to and biofilm formation on polystyrene byStenotrophomonas maltophilia: the role of cell surface hydrophobicity and motility

2008 ◽  
Vol 287 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Arianna Pompilio ◽  
Raffaele Piccolomini ◽  
Carla Picciani ◽  
Domenico D'Antonio ◽  
Vincenzo Savini ◽  
...  
Keyword(s):  
Cell Surface ◽  
Biofilm Formation ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Factors Associated

Insight into heterogeneity in cell-surface hydrophobicity and ability to degrade hydrocarbons among cells of two hydrocarbon-degrading bacterial populations

2007 ◽  
Vol 53 (2) ◽  
pp. 252-260 ◽  
Author(s):  
Christian O. Obuekwe ◽  
Zamya K. Al-Jadi ◽  
Esmaeil Al-Saleh
Keyword(s):  
Cell Surface ◽  
Burkholderia Cepacia ◽  
Natural Populations ◽  
Cell Surface Hydrophobicity ◽  
Hydrophobic Surface ◽  
Surface Hydrophobicity ◽  
Surface Distribution ◽  
Bacterial Populations ◽  
Paenibacillus Sp ◽  
Successive Generations

The sequential bacterial adherence to hydrocarbons (BATH) of successive generations of hydrophobic fractions of Paenibacillus sp. R0032A and Burkholderia cepacia gave rise to bacterial populations of increasing cell-surface hydrophobicity. Thus, hydrophobicity of the first generation (H1) was less than that of the second generation (H2), which was less than that of the third generation (H3). Beyond H3, the hydrophobic populations became less stable and tended to lyse in hexadecane after violent (vortex) agitation, resulting in an apparent decline in BATH value. The exhaustively fractionated aqueous-phase population (L) was very hydrophilic. The overall cell-surface distribution of the population was L < parental strain < H1 < H2 < H3. The ability to degrade crude oil, hexadecane, or phenanthrene matched the degree of cell-surface hydrophobicity: L < P < H1 < H2 < H3. Thus, in natural populations of hydrocarbon-degrading Paenibacillus sp. R0032A and B. cepacia, there is a heterogeneity in the hydrophobic surface characteriistics that affects the ability of cells to use various hydrocarbon substrates.

scholarly journals Role of Cell Surface Hydrophobicity in the Pathogenesis of Medically-Significant Fungi

2021 ◽  
Vol 10 ◽  
Author(s):  
Carina Danchik ◽  
Arturo Casadevall
Keyword(s):  
Cell Surface ◽  
Cell Surface Hydrophobicity ◽  
Pathogenic Fungi ◽  
Surface Hydrophobicity ◽  
Dimorphic Fungi ◽  
Biophysical Parameter ◽  
Filamentous Cell ◽  
Cell Surface Interactions ◽  
Mold And Yeast

Cell surface hydrophobicity (CSH) is an important cellular biophysical parameter which affects both cell-cell and cell-surface interactions. In dimorphic fungi, multiple factors including the temperature-induced shift between mold and yeast forms have strong effects on CSH with higher hydrophobicity more common at the lower temperatures conducive to filamentous cell growth. Some strains of Cryptococcus neoformans exhibit high CSH despite the presence of the hydrophilic capsule. Among individual yeast colonies from the same isolate, distinct morphologies can correspond to differences in CSH. These differences in CSH are frequently associated with altered virulence in medically-significant fungi and can impact the efficacy of antifungal therapies. The mechanisms for the maintenance of CSH in pathogenic fungi remain poorly understood, but an appreciation of this fundamental cellular parameter is important for understanding its contributions to such phenomena as biofilm formation and virulence.

Role of cell surface hydrophobicity in Candida albicans biofilm

2013 ◽  
Vol 8 (3) ◽  
pp. 259-262 ◽  
Author(s):  
Helena Bujdáková ◽  
Miroslava Didiášová ◽  
Hana Drahovská ◽  
Lucia Černáková
Keyword(s):  
Gene Expression ◽  
Candida Albicans ◽  
Cell Surface ◽  
Biofilm Formation ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Quantitative Real Time Pcr ◽  
Planktonic Cells ◽  
Planktonic Culture

AbstractOverall cell surface hydrophobicity (CSH) is predicted to play an important role during biofilm formation in Candida albicans but is the result of many expressed proteins. This study compares the CSH status and CSH1 gene expression in C. albicans planktonic cells, sessile biofilm, and dispersal cells. Greater percentages of hydrophobic cells were found in non-adhered (1.5 h) and dispersal forms (24 or 48 h) (41.34±4.17% and 39.52±7.45%, respectively), compared with overnight planktonic cultures (21.69±3.60%). Results from quantitative real-time PCR confirmed greater up-regulation of the CSH1 gene in sessile biofilm compared with both planktonic culture and dispersal cells. Up-regulation was also greater in dispersal cells compared with planktonic culture. The markedly increased CSH found both in C. albicans biofilm, and in cells released during biofilm formation could provide an advantage to dispersing cells building new biofilm.

scholarly journals Heterologous Inducible Expression ofEnterococcus faecalis pCF10 Aggregation Substance Asc10 inLactococcus lactis and Streptococcus gordonii Contributes to Cell Hydrophobicity and Adhesion to Fibrin

2000 ◽  
Vol 182 (8) ◽  
pp. 2299-2306 ◽  
Author(s):  
Helmut Hirt ◽  
Stanley L. Erlandsen ◽  
Gary M. Dunny
Keyword(s):  
Cell Surface ◽  
Cell Surface Hydrophobicity ◽  
Regulatory System ◽  
Surface Hydrophobicity ◽  
Bacterial Cells ◽  
Streptococcus Gordonii ◽  
Cell Surfaces ◽  
Gene Encoding ◽  
Aggregation Substance

ABSTRACT Aggregation substance proteins encoded by the sex pheromone plasmid family of Enterococcus faecalis have been shown previously to contribute to the formation of a stable mating complex between donor and recipient cells and have been implicated in the virulence of this increasingly important nosocomial pathogen. In an effort to characterize the protein further, prgB, the gene encoding the aggregation substance Asc10 on pCF10, was cloned in a vector containing the nisin-inducible nisA promoter and its two-component regulatory system. Expression of aggregation substance after nisin addition to cultures of E. faecalis and the heterologous bacteria Lactococcus lactis andStreptococcus gordonii was demonstrated. Electron microscopy revealed that Asc10 was presented on the cell surfaces ofE. faecalis and L. lactis but not on that ofS. gordonii. The protein was also found in the cell culture supernatants of all three species. Characterization of Asc10 on the cell surfaces of E. faecalis and L. lactisrevealed a significant increase in cell surface hydrophobicity upon expression of the protein. Heterologous expression of Asc10 on L. lactis also allowed the recognition of its binding ligand (EBS) on the enterococcal cell surface, as indicated by increased transfer of a conjugative transposon. We also found that adhesion of Asc10-expressing bacterial cells to fibrin was elevated, consistent with a role for the protein in the pathogenesis of enterococcal endocarditis. The data demonstrate that Asc10 expressed under the control of the nisA promoter in heterologous species will be an useful tool in the detailed characterization of this important enterococcal conjugation protein and virulence factor.

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