Seroepidemiology of Q fever in New Brunswick and Manitoba

1988 ◽  
Vol 34 (9) ◽  
pp. 1043-1045 ◽  
Author(s):  
Thomas J. Marrie
Keyword(s):  
Phase I ◽  
New Brunswick ◽  
Antibody Titer ◽  
Phase Ii ◽  
Coxiella Burnetii ◽  
Blood Donors ◽  
Q Fever ◽  
Immunofluorescence Test ◽  
Serum Samples ◽  
Indirect Immunofluorescence Test

A seroepidemiological survey, using an indirect immunofluorescence test, was carried out on serum samples obtained from New Brunswick and Manitoba blood donors during 1986. The antigens were Coxiella burnetii phase I and phase II from strain Nine Mile. Eighty of the 503 (15.9%) Manitoba blood donors had a phase II antibody titer of ≥ 1:8, while 41 (4.2%) of the 966 New Brunswick blood donors had such antibodies. We have recently diagnosed three cases of Q fever in New Brunswick but none have been diagnosed in Manitoba. Our data suggest that Q fever may be increasing in New Brunswick and repeated seroepidemiological studies are indicated. It is likely that undetected cases of Q fever are occurring in Manitoba.

scholarly journals Seroprevalence Coxiella burnetii among cows and sheep in Thi-Qar Province/ Iraq

2010 ◽  
Vol 9 (2) ◽  
pp. 26
Author(s):  
Journal Manager ◽  
J. Abed, A.A Salih, and A. Abd-ul-husien
Keyword(s):  
Phase I ◽  
Phase Ii ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Serum Samples

The aim of this study is detecting antibodies of phase I and phase II of Coxiella burnetii bacterium, the cause of Q-fever, a zoonotic diesase in humans and animals in Thi-Qar province.Out of 393 serum samples collected randomly from Thi-Qar province from aborted and non aborted cows and ewes, the results appeared that 29 (7.37%) samples of cows and ewes were seropositive for C. burnetii distributed as 16 seropositive samples of 172 cows (9.3%) and 13 seropositive samples of 221 sheep (5.8%).the most positive cases associated with abortion cases with ratio (92.3%) in ewes and (75%) in cows.

scholarly journals Animals withCoxiella burnetiiInfection Demonstrate a Western Immunoblot Profile of Chronic Q Fever in Humans

1996 ◽  
Vol 7 (1) ◽  
pp. 45-48
Author(s):  
TJ Marrie ◽  
Linda Yates
Keyword(s):  
Immune Response ◽  
Phase I ◽  
Phase Ii ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Western Immunoblotting ◽  
Western Immunoblot ◽  
Chronic Q Fever

Western immunoblotting was used to compare the immune response toCoxiella burnetiiphase I and phase II antigens of humans with acute and chronic Q fever with that of infected cats, rabbits, cows and raccoons. The cats, rabbits, cows and raccoons had an immunoblot profile similar to that of the human with chronic Q fever.

scholarly journals Q fever in the Netherlands: a sero-epidemiological survey among human population groups from 1968 to 1983

1987 ◽  
Vol 98 (2) ◽  
pp. 211-219 ◽  
Author(s):  
J. H. Richardus ◽  
A. Donkers ◽  
A. M. Dumas ◽  
G. J. P. Schaap ◽  
J. P. W. M. Akkermans ◽  
...  
Keyword(s):  
The Netherlands ◽  
Indirect Immunofluorescence ◽  
Q Fever ◽  
Epidemiological Survey ◽  
Immunofluorescence Test ◽  
Age Classes ◽  
Serum Samples ◽  
Occupational Groups ◽  
Indirect Immunofluorescence Test ◽  
Long Time

SUMMARYA sero-epidemiological survey, using an indirect immunofluorescence test for IgG against Coxiella burnetii (phase II), was carried out in the Netherlands. Serum samples taken in 1968, 1975, 1979 and 1983 were tested. Occupational groups with a supposedly high risk of infection (veterinarians, residents of dairy farms and taxidermists) showed a significantly higher percentage of seropositives than defined controls. The percentage of seropositive amateur wool spinners was significantly higher than that of the controls from the same region. Since 1968 there has been no increase in the percentage of infected persons, indicating that, contrary to earlier assumptions, Q fever has been endemic in The Netherlands for a long time already. The increase in numbers of notified cases of overt Q fever is considered to be the result of the recent introduction of a sensitive indirect immunofluorescence test for IgM antibodies against C. burnetii. Antibody percentages in all age classes between 1 and 64 years were much alike, suggesting that most infections occur in early childhood. This is in accordance with the finding that 35% of our patients are younger than 3 years. The possibility of infection related to childbirth and lactation is discussed.

scholarly journals Truckin' pneumonia—an outbreak of Q fever in a truck repair plant probably due to aerosols from clothing contaminated by contact with newborn kittens

1989 ◽  
Vol 102 (1) ◽  
pp. 119-127 ◽  
Author(s):  
Thomas J. Marrie ◽  
Donald Langille ◽  
Vasilia Papukna ◽  
Linda Yates
Keyword(s):  
Phase I ◽  
Phase Ii ◽  
Coxiella Burnetii ◽  
Attack Rate ◽  
Q Fever ◽  
The Other ◽  
High Antibody ◽  
First Case ◽  
The Family ◽  
Antibody Titres

SUMMARYWe describe an outbreak of Q fever affecting 16 of 32 employees at a truck repair plant. None of the cases were exposed to cattle, sheep or goats. the traditional reservoirs of Q fever. The cases did not work, live on, or visit farms or attend livestock auctions. One of the employees had a cat which gave birth to kittens 2 weeks prior to the first case of Q fever in the plant. The cat owner fed the kittens every day before coming to work as the cat would not let the kittens suckle. Serum from the cat had high antibody titres to phase I and phase IICoxiella burnetiiantigens. The attack rate among the employees where the cat owner worked. 13 of 19 (68%), was higher than that of employees elsewhere, 3 of 13 (28%) [P <0·01]. The cat owner's wife and son also developed Q fever. None of the family members of the other employees with Q fever was so affected.We conclude that this outbreak of Q fever probably resulted from exposure to the contaminated clothing of the cat owner.

Seroepidemiology of Q fever in Nova Scotia and Prince Edward Island

1984 ◽  
Vol 30 (1) ◽  
pp. 129-134 ◽  
Author(s):  
Thomas J. Marrie ◽  
John Van Buren ◽  
Ruth S. Faulkner ◽  
E. Vanora Haldane ◽  
James C. Williams ◽  
...  
Keyword(s):  
Nova Scotia ◽  
Phase I ◽  
Phase Ii ◽  
Prince Edward Island ◽  
Blood Donors ◽  
Complement Fixation ◽  
Q Fever ◽  
Antibody Prevalence ◽  
Seroepidemiologic Studies ◽  
Microimmunofluorescence Test

The prevalence of Coxiella burnetti infection (Q fever) was determined among Nova Scotia (N.S.) and Prince Edward Island (P.E.I.) blood donors by using the complement fixation and microimmunofluorescence (IF) test. The complement fixation and IF antibody tests measured antibody prevalence for the phase II or phase I and II antigens, respectively. Complement-fixing antibodies to phase II antigen were detected in 4.1% of 997 N.S. and 5.0% of 219 P.E.I. blood donors. Anti-phase II antibodies were detected by microimmunofluorescence in 11.8 and 14.6% of the blood donors in the two provinces, respectively. Anti-phase I antibodies were detected among 2.8% of the N.S. blood donors and 6.3% of the P.E.I. blood donors. Comparison of rates of anti-phase II IF by counties in N.S. revealed that there was at least one county where infection by C. burnetti is hyperendemic. Rates of antibody prevalence were similar in all three areas of P.E.I. examined. We conclude that "Q fever" is endemic in N.S. and P.E.I. and that the microimmunofluorescence test is more suitable than the complement fixation test for seroepidemiologic studies.

scholarly journals Early Diagnosis and Treatment of Patients with Symptomatic Acute Q Fever Do Not Prohibit IgG Antibody Responses to Coxiella burnetii

2012 ◽  
Vol 19 (10) ◽  
pp. 1661-1666 ◽  
Author(s):  
C. C. H. Wielders ◽  
L. M. Kampschreur ◽  
P. M. Schneeberger ◽  
M. M. Jager ◽  
A. I. M. Hoepelman ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Phase I ◽  
Phase Ii ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Antibody Responses ◽  
Igg Antibody ◽  
Antibody Titers ◽  
Acute Q Fever

ABSTRACTLittle is known about the effect of timing of antibiotic treatment on development of IgG antibodies following acute Q fever. We studied IgG antibody responses in symptomatic patients diagnosed either before or during development of the serologic response toCoxiella burnetii. Between 15 and 31 May 2009, 186 patients presented with acute Q fever, of which 181 were included in this retrospective study: 91 early-diagnosed (ED) acute Q fever patients, defined as negative IgM phase II enzyme-linked immunosorbent assay (ELISA) and positive PCR, and 90 late-diagnosed (LD) acute Q fever patients, defined as positive/dubious IgM phase II ELISA and positive immunofluorescence assay (IFA). Follow-up serology at 3, 6, and 12 months was performed using IFA (IgG phase I and II). High IgG antibody titers were defined as IgG phase II titers of ≥1:1,024 together with IgG phase I titers of ≥1:256. At 12 months, 28.6% of ED patients and 19.5% of LD patients had high IgG antibody titers (P= 0.17). No statistically significant differences were found in frequencies of IgG phase I and IgG phase II antibody titers at all follow-up appointments for adequately and inadequately treated patients overall, as well as for ED and LD patients analyzed separately. Additionally, no significant difference was found in frequencies of high antibody titers and between early (treatment started within 7 days after seeking medical attention) and late timing of treatment. This study indicates that early diagnosis and antibiotic treatment of acute Q fever do not prohibit development of the IgG antibody response.

scholarly journals Seroprevalence of Q fever in small ruminants in the northeast Anatolian region in Turkey

2021 ◽  
Vol 77 (05) ◽  
pp. 6522-2021
Author(s):  
PERIHAN SERIFOĞLU BAGATIR ◽  
BIRAY OKUMUS ◽  
EDIZ KAAN OZGEN ◽  
MUSTAFA ULUCAN ◽  
BERNA YANMAZ ◽  
...  
Keyword(s):  
Phase I ◽  
Coxiella Burnetii ◽  
Indirect Elisa ◽  
Q Fever ◽  
Small Ruminants ◽  
Animal Husbandry ◽  
Fold Increase ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Goat Population

The aim of our study was to determine the seroepidemiological profile of Q fever in small ruminants in Turkey and to examine its prevalence changes over the years. The study included 573 serum samples taken in 2013 and 472 samples taken in 2017 from animals in mixed herds of sheep and goats from 84 farms in Northeast Anatolia. Phase I and phase II IgG antibodies against Coxiella burnetii in serum samples were investigated by IDEXX ELISA (Q fever Ab Test IDEXX Laboratories, USA) indirect ELISA kits. Seroprevalence of Coxiella burnetii IgG in Artvin, Gümüşhane and Iğdır provinces was 5.6% in sheep, 1.8% in goats and 4.5% in total in 2013. In contrast, it was 24.4% in sheep, 1.1% in goats and 20.1% in total in 2017. According to the total seroprevalence rates calculated by including both sheep and goat population, it was seen that the province with the highest seroprevalence change in these animals was Iğdır with a 7.3-fold increase. Herd-level seroprevalence was 29.4% in 2013 and 57.6% in 2017. According to these results, the C. burnetii IgG seroprevalence nearly doubled after four years. This increase has been evaluated as a major risk for animal and human health as well as for the livestock economy in Northeastern Anatolia, where animal husbandry is intense.

scholarly journals Culture under normoxic conditions and enhanced virulence of phase II Coxiella burnetii transformed with a RSF1010-based shuttle vector

2019 ◽  
Author(s):  
Shengdong Luo ◽  
Zemin He ◽  
Zhihui Sun ◽  
Yonghui Yu ◽  
Yongqiang Jiang ◽  
...  
Keyword(s):  
Phase I ◽  
Phase Ii ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Shuttle Vector ◽  
Axenic Culture ◽  
Hypoxic Condition ◽  
Kanamycin Resistance ◽  
Temperature Sensitive ◽  
Egfp Gene

AbstractCoxiella burnetii is a Gram-negative, facultative intracellular microorganism that can cause acute or chronic Q fever in human. It was recognized as an obligate intracellular organism until the revolutionary design of an axenic cystine culture medium (ACCM). Present axenic culture of C. burnetii strictly requires a hypoxic condition (<10% oxygen). Here we investigated the normoxic growth of C. burnetii strains in ACCM-2 with or without tryptophan supplementation. Three C. burnetii strains - Henzerling phase I, Nine Mile phase II and a Nine Mile phase II transformant, were included. The transformant contains a pMMGK plasmid that is composed of a RSF1010 ori, a repABC operon, an eGFP gene and a kanamycin resistance cassette. We found that, under normoxia if staring from an appropriate concentration of fresh age inocula, Nine Mile phase II can grow significantly in ACCM-2 with tryptophan, while the transformant can grow robustly in ACCM-2 with or without tryptophan. In contrast, long-term frozen stocks of phase II and its transformant, and Henzerling phase I of different ages had no growth capability under normoxia under any circumstances. Furthermore, frozen stocks of the transformant consistently caused large splenomegaly in SCID mice, while wild type Nine Mile phase II induced a lesser extent of splenomegaly. Taken together, our data show that normoxic cultivation of phase II C. burnetii can be achieved under certain conditions. Our data suggests that tryptophan and an unknown temperature sensitive signal are involved in the expression of genes for normoxic growth regulated by quorum sensing in C. burnetii.

Infection by Coxiella burnetii in a patient from a rural area of Monteria, Colombia

2015 ◽  
Vol 16 (6) ◽  
pp. 958-961 ◽  
Author(s):  
Salim Mattar V ◽  
Verónica Contreras C ◽  
Marco Gonzalez T ◽  
Francisco Camargo ◽  
Jaime Alvarez ◽  
...  
Keyword(s):  
Phase I ◽  
Rural Area ◽  
Phase Ii ◽  
Coxiella Burnetii ◽  
Indirect Immunofluorescence ◽  
Q Fever ◽  
Immunofluorescence Assay ◽  
Indirect Immunofluorescence Assay ◽  
Igg Antibody ◽  
Antibody Titers

<p>Q fever is a zoonosis caused by <em>Coxiella burnetii</em>. In Colombia, there have been very few human cases reported to date. This report describes the case of a 56-year-old patient with a background in agriculture and livestock handling. An indirect immunofluorescence assay (IFA) showed high titers of IgG for <em>C. burnetii</em> anti-phase I (1: 256) and anti-phase II (1:1024). For the next six months the patient’s IgG antibody titers remained high, and, after treatment with doxycycline, the IgG antibody titers decreased to 50 % (anti-phase I 1:128 and anti-phase II 1:512); this profile suggests an infection of <em>C. burnetii</em>.</p>

Proteomic comparison of virulent phase I and avirulent phase II of Coxiella burnetii, the causative agent of Q fever

2011 ◽  
Vol 74 (10) ◽  
pp. 1974-1984 ◽  
Author(s):  
Ludovit Skultety ◽  
Martin Hajduch ◽  
Gabriela Flores-Ramirez ◽  
Ján A. Miernyk ◽  
Fedor Ciampor ◽  
...  
Keyword(s):  
Phase I ◽  
Phase Ii ◽  
Coxiella Burnetii ◽  
Causative Agent ◽  
Q Fever
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