Genetic and physical characterization of trimethoprim resistance plasmids from Shigella sonnei and Shigella flexneri

1987 ◽  
Vol 33 (10) ◽  
pp. 905-913 ◽  
Author(s):  
Patricia N. Tonin ◽  
Robert B. Grant
Keyword(s):  
Genetic Characterization ◽  
Shigella Flexneri ◽  
Chloride Ions ◽  
Multiple Antibiotic Resistance ◽  
The Third ◽  
Resistance Plasmids ◽  
Restriction Endonuclease Fragment ◽  
Incompatibility Reaction ◽  
Representative Member

Analysis of six Shigella flexneri and four S. sonnei isolates with trimethoprim (Tp) resistance from clinical cases in Ontario has shown that, in all isolates, the Tp resistance is mediated by gene(s) on conjugative, multiple antibiotic-resistance plasmids. The physical and genetic characterization of these plasmids revealed that there are three different Tp resistance plasmids. One group, composed of all six S. flexneri plasmids, consists of plasmids which are about 70 megadaltons (MDa) and inhibit the fertility of an Escherichia coli Hfr strain (Fi+). A representative member of this group, pPT4, demonstrates a weak incompatibility reaction with IncFI plasmid R455-2. Another group, three of the four S. sonnei plasmids, contains plasmids which are about 43 MDa, Fi−, and mediate propagation of phage PRD1. The third group, the remaining S. sonnei plasmid, is 53 MDa,fi+, mediates propagation of phages fd and MS2, and is incompatible with IncFII plasmid R100. These plasmids also have been differentiated by restriction endonuclease fragment profiles. Analysis of pPT4 has revealed that the Tp resistance of this plasmid is transposable. The transposon, Tn536, is different from previously described Tp resistance transposons; it is 16 MDa, and in addition to Tp, it encodes resistance to mercuric chloride ions, spectinomycin, streptomycin, and sulfonamides.

scholarly journals Chordoid Glioma of the Third Ventricle: Immunohistochemical and Molecular Genetic Characterization of a Novel Tumor Entity

1999 ◽  
Vol 9 (4) ◽  
pp. 617-626 ◽  
Author(s):  
Guido Reifenberger ◽  
Tillmann Weber ◽  
Ruthild G. Weber ◽  
Marietta Wolter ◽  
Almuth Brandis ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Third Ventricle ◽  
Molecular Genetic ◽  
The Third ◽  
Tumor Entity ◽  
Chordoid Glioma ◽  
Molecular Genetic Characterization

scholarly journals Genetic Characterization of Six Stocks ofLitopenaeus vannameiUsed in Cuba for Aquaculture by Means of Microsatellite Loci

2012 ◽  
Vol 2012 ◽  
pp. 1-7
Author(s):  
Anna Pérez-Beloborodova ◽  
Adriana Artiles-Valor ◽  
Lourdes Pérez-Jar ◽  
Damir Hernández-Martínez ◽  
Missael Guerra-Aznay ◽  
...  
Keyword(s):  
Litopenaeus Vannamei ◽  
Microsatellite Loci ◽  
First Generation ◽  
Second Generation ◽  
Genetic Characterization ◽  
Parental Population ◽  
Allelic Variants ◽  
The Third ◽  
Private Alleles

Four microsatellite loci were used to achieve genetic characterization of six stocks fromLitopenaeus vannameiused for aquaculture in Cuba: second generation from first introduction (S2-1), first generation from the second one (S1-2), from the third one (S1-3), and the fourth one (S1-4) and the crossings from two parental population: first generation from the first with first generation from the third (S1-1 × S1-3) and first generation from the second with first generation from the third (S1-2 × S1-3). 66% (16/24) of genetic systems in total loci were in genetic disequilibrium. The four microsatellite loci were polymorphic for all six stocks. Major quantities of allelic variants correspond to locus Pvan 1758, which is at the same time that one where there are private alleles from first generation of the third. All Fst comparisons were significant. This indicates big differences between stocks. The highest values are those in which there is presence of the second introduction. This introduction and its descendants are also more consanguineous.

scholarly journals Genetic characterization of antimicrobial resistance of Shigella flexneri 1c isolates from patients in Egypt and Pakistan

2013 ◽  
Vol 12 (1) ◽  
pp. 9 ◽  
Author(s):  
Salwa F Ahmed ◽  
John Klena ◽  
Tupur Husain ◽  
Jesse Monestersky ◽  
Amel Naguib ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Genetic Characterization ◽  
Shigella Flexneri

scholarly journals Epidemiology and genetic characterization of Shigella flexneri strains isolated from three paediatric populations in Egypt (2000–2004)

2006 ◽  
Vol 134 (6) ◽  
pp. 1237-1248 ◽  
Author(s):  
S. F. AHMED ◽  
M. S. RIDDLE ◽  
T. F. WIERZBA ◽  
I. ABDEL MESSIH ◽  
M. R. MONTEVILLE ◽  
...  
Keyword(s):  
Medical Care ◽  
Genetic Characterization ◽  
Shigella Flexneri ◽  
Geographical Region ◽  
Pulsed Field ◽  
Pulsed Field Electrophoresis ◽  
Marked Resistance

Ninety-seven isolates of Shigella flexneri from children seeking medical care from three sites in Egypt were characterized. Overall, 46·4% of children (median age 17 months) were febrile or reported blood in their stools, 25·8% were dehydrated and 16·5% were admitted to hospital. Serotypes 2a (37·1%), 1b (18·6%), 1c (17·5%), and 6 (15·5%) comprised over 88·7% of the total isolates. We observed marked resistance to ampicillin (87·6%), tetracycline (84·5%) and trimethoprim–sulfamethoxazole (63·9%). Pulsed-field electrophoresis grouped the majority of isolates within a serotype together, separately from isolates of an alternative serotype. The set gene was present in all serogroup 2a isolates, however, the sen gene was detected in every isolate. Our results show S. flexneri 1c has emerged as a dominant S. flexneri serotype in Egypt. Development and application of a Shigella vaccine should consider the diversity of Shigella serotypes within a geographical region prior to administration.

scholarly journals GENETIC CHARACTERIZATION OF THE 87C REGION OF THE THIRD CHROMOSOME OF DROSOPHILA MELANOGASTER

Genetics ◽  
1979 ◽  
Vol 93 (4) ◽  
pp. 917-934 ◽  
Author(s):  
Janos Gausz ◽  
Gabor Bencze ◽  
Henrik Gyurkovics ◽  
Michael Ashburner ◽  
David Ish-Horowicz ◽  
...  
Keyword(s):  
Heat Shock ◽  
Genetic Characterization ◽  
Break Point ◽  
Complementation Group ◽  
Coding Sequences ◽  
The Third ◽  
Functional Locus ◽  
Group A ◽  
Complementation Groups

ABSTRACT Ethyl methanesulphonate (EMS) was used to induce 39 lethal and 13 karmoisin mutations within Df(3R)kar3J, a nine-band deficiency extending from 87C1 to 87C9 (inclusive). Five complementation groups (four lethal and one visible) were identified and cytologically mapped between 8764-5 and 87C9, one complementation group per band, with the exception of complementation group A, which is localized to 87C4-5. These positions were determined using a set of overlapping deficiencies, each having at least one break-point in the 87C1-9 region. Mutations within a single complementation group have similar lethal phases or subvital phenotypes, consistent with the notion that each complementation group represents a single functional locus. No mutations localized to 87CI-C3. The inability to induce mutations in the 87C1 heat-shock puff locus is consistent with the current interpretation of a duplication of coding sequences at the 87A7 and 87C1 heat-shock puffs.

scholarly journals Genetic analysis of the heterochromatin of chromosome 3 in Drosophila melanogaster. I. Products of compound-autosome detachment.

Genetics ◽  
1988 ◽  
Vol 120 (2) ◽  
pp. 503-517
Author(s):  
G E Marchant ◽  
D G Holm
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Analysis ◽  
Genetic Characterization ◽  
Complementation Analysis ◽  
Chromosome 3 ◽  
Recessive Lethal ◽  
The Third ◽  
Left And Right ◽  
The Right

Abstract The heterochromatin of the third chromosome is the largest uncharacterized region of the Drosophila melanogaster genome, and the last major block of D. melanogaster heterochromatin to be thoroughly analyzed. In the present study, this region was genetically dissected by generating and analyzing a series of attached, detached and reattached third chromosomes. Separate detachment experiments were conducted for all 12 possible combinations of four newly synthesized sister-strand compound-3L and three newly synthesized sister-strand compound-3R chromosomes. A total of 443 recessive lethal detachment products carrying putative heterochromatic deficiencies were tested for complementation in a several-stage complementation analysis. The results revealed the presence of seven separable vital regions in the heterochromatin of chromosome 3. Attempts to reattach deficiency-carrying detachment products established that six of these vital regions are on the left arm, but only one is on the right arm. An analysis of the types and frequencies of detachment-product deficiencies generated in each detachment experiment permitted the genetic characterization of the progenitor compounds. It was also possible to determine the proximal-distal orientation of the genes on each arm, and to identify possible breakpoints for each lethal detachment product produced. The results of this study suggest that vital genes in the heterochromatin of the third chromosome are not randomly distributed between, nor within, the heterochromatic blocks of the left and right arms.

scholarly journals Genetic Characterization of Shigella flexneri Isolates in Guizhou Province, China

PLoS ONE ◽  
2015 ◽  
Vol 10 (1) ◽  
pp. e0116708 ◽  
Author(s):  
Shijun Li ◽  
Qiangzheng Sun ◽  
Xiaoyu Wei ◽  
John D. Klena ◽  
Jianping Wang ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Shigella Flexneri ◽  
Guizhou Province

scholarly journals Genetic characterization of three qnrS1-harbouring multidrug-resistance plasmids and qnrS1-containing transposons circulating in Ho Chi Minh City, Vietnam

2015 ◽  
Vol 64 (8) ◽  
pp. 869-878 ◽  
Author(s):  
Vien Le ◽  
Nguyen Thi Khanh Nhu ◽  
Ana Cerdeno-Tarraga ◽  
James I. Campbell ◽  
Ha Thanh Tuyen ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Genetic Characterization ◽  
Ho Chi Minh City ◽  
Resistance Plasmids
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