Biosynthesis of trans fatty acids from acetate in the bacterium Pseudomonas atlantica

1987 ◽  
Vol 33 (9) ◽  
pp. 748-754 ◽  
Author(s):  
James B. Guckert ◽  
David B. Ringelberg ◽  
David C. White
Keyword(s):  
Fatty Acids ◽  
Biosynthetic Pathway ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Test Organism ◽  
Membrane Fatty Acids ◽  
Ecological Implications ◽  
Starvation Survival ◽  
Adhesion Process

The cis configuration in fatty acids is the only known product of bacterial biosynthetic pathways of monounsaturated membrane fatty acids. The trans configuration is considered "nonphysiologic" or "unnatural." This research shows that in vivo synthesis of trans-monounsaturated membrane fatty acids from acetate occurs in the bacterium Pseudomonas atlantica. The saturated, trans, and cis phospholipid fatty acids (PLFA) of P. atlantica grown in the presence of [14C]acetate were physically separated as the corresponding fatty acid methyl esters (FAME) and the presence of trans FAME was verified by infrared spectroscopy. The FAME isomers were quantified by gas chromatography and 14C incorporation into these isomers was counted. The specific activities of trans and saturated PLFA were found to be equivalent, indicating that P. atlantica has the in vivo biosynthetic capabilities to synthesize trans PLFA from acetate. From these results, a biosynthetic pathway is hypothesized and the suggestion is made that P. atlantica would be a preferred test organism to elucidate this pathway as well as to test the ecological implications of these trans PLFA in terms of starvation survival and the initial biofouling–adhesion process.

Determination of cis,cis-Methylene Interrupted Polyunsaturated Fatty Acids in Fats and Oils By Capillary Gas Chromatography

1986 ◽  
Vol 69 (1) ◽  
pp. 65-67
Author(s):  
Albert K Athnasios ◽  
Edward J Healy ◽  
Anthony F Gross ◽  
Gareth J Templeman
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Fatty Acid Methyl Esters ◽  
Capillary Column ◽  
Methyl Esters ◽  
Fats And Oils ◽  
Pufa Content ◽  
Acid Methyl ◽  
Relative Standard

Abstract A capillary gas chromatographic (CGC) method is described for the determination of cis,cis-methylene interrupted polyunsaturated fatty acids (cis-PUFA) in fats and oils. The sample is saponified and the liberated fatty acids are esterified to the corresponding methyl esters. The latter are analyzed by CGC using a 60M SP2340 capillary column. Area percent values for 9,12-cis,cis-C18:2 and 9,12,15-cis,cis,cis-C18:3 fatty acid methyl esters are summed to give the total cis-PUFA content. Gas chromatographic results agreed well with those obtained by an enzymatic lipoxygenase method at the 31-48% cis-PUFA levels with a correlation coefficient of 0.98. The method has a precision (relative standard deviation) of 0.33% at a 44.4% cis-PUFA level in margarine oil.

Chloroplast Fatty Acid Composition in Mediterranean Populations of the Marine Chlorophyte, Anadyomene stellata

2000 ◽  
Vol 55 (7-8) ◽  
pp. 569-575 ◽  
Author(s):  
Debra L. Bemis ◽  
Vassilios Roussis ◽  
Constantinios Vagias ◽  
Robert S. Jacobs
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Florida Keys ◽  
Mediterranean Populations ◽  
Total Fatty Acids ◽  
Of Greece

Abstract Chloroplasts isolated from three populations of the tropical marine Chlorophyte Anadyomene stellata collected off the coast of Greece were analyzed for their fatty acid composition. Following the preparation of fatty acid methyl esters, GC-MS (El) was utilized to identify the fatty acids present in each population. Including isomers, the fatty acid profile of all three algal populations was comprised of 19 fatty acids (4 saturated, 6 monounsaturated, 9 polyunsaturated) with palmitic acid present in the highest amounts (25-27% of total fatty acids). Analysis of variance revealed significant differences amongst the three populations in the percent of total fatty acids for twelve of the fatty acids. High levels of C20 PUFAs, an atypical observation in Chlorophytes, were observed in all three populations comprising approximately 17% of total fatty acids. Furthermore a 14:2 PUFA , apparently rare in marine macrophytic Chlorophytes, was identified in significant quantities. Surprisingly, we did not find any of the conjugated tetraene containing fatty acids that we previously identified in the A. stellata populations studied from the Florida Keys.

Recent advances in the field of selective epoxidation of vegetable oils and their derivatives: a review and perspective

2017 ◽  
Vol 7 (17) ◽  
pp. 3659-3675 ◽  
Author(s):  
S. M. Danov ◽  
O. A. Kazantsev ◽  
A. L. Esipovich ◽  
A. S. Belousov ◽  
A. E. Rogozhin ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Vegetable Oils ◽  
Critical Review ◽  
Fatty Acid Methyl Esters ◽  
Recent Progress ◽  
Unsaturated Fatty Acids ◽  
Methyl Esters ◽  
Acid Methyl ◽  
Review Reports

The present critical review reports the recent progress of the last 15 years in the selective epoxidation of vegetable oils and their derivatives, in particular unsaturated fatty acids (UFAs) and fatty acid methyl esters (FAMEs).

Direct ultrasound-assisted methylation of fatty acids in serum for free fatty acid determinations

2010 ◽  
Vol 88 (9) ◽  
pp. 898-905 ◽  
Author(s):  
Liyan Liu ◽  
Ying Li ◽  
Rennan Feng ◽  
Changhao Sun
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Ultrasonic Waves ◽  
Acid Methyl ◽  
Ultrasound Assisted

A method for simultaneous determination of 16 free fatty acids (FFAs) in serum is described. The method involves conversion of FFAs to fatty acid methyl esters (FAMEs) using the heat of ultrasonic waves followed by gas chromatography and mass spectrometry (GC–MS) analysis. Optimum levels of the variables affecting the yield of FAMEs were investigated. The results indicate that the optimal levels are 55 °C, 60 W, 10% H2SO4/CH3OH, and 50 min. Recoveries ranged from 85.32% to 112.11%, with a detection limit ranging from 0.03 to 0.08 μg mL–1. The linearity, using the linear correlation coefficient, was higher than 0.9914.

scholarly journals Markers of quality and genuineness of commercial extra virgin sacha inchi oils

2016 ◽  
Vol 67 (4) ◽  
pp. 169 ◽  
Author(s):  
N. A. Chasquibol ◽  
R. B. Gómez-Coca ◽  
J. C. Yácono ◽  
Á. Guinda ◽  
W. Moreda ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Physicochemical Parameters ◽  
Trans Fatty Acids ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Minor Components ◽  
Basic Requirement ◽  
Acid Methyl ◽  
Sacha Inchi Oil ◽  
Sacha Inchi

This work tackles the study of the quality and authenticity of oils labeled and commercialized as extra virgin sacha inchi oil. Major and minor components as triglycerides, fatty acid methyl esters, tocopherols, sterols and hydrocarbons are determined as well as other physicochemical parameters (density, viscosity, acidity and peroxide value). The results showed that some of the commercialized oils do not fulfill the basic requirement established in the regulation such as the content of α-linolenic acid, higher than 44.7 or 55.0% in the cases of P. volubilis and P. huayllabambana, respectively. The calculated stigmasterol/campesterol ratio for genuine sacha inchi oils should be around 4, however not all commercial oils analyzed comply with this requirement. The presence of the flavons sesamin and sesamolin indicates the addition of compounds from sesame oils. Finally, some of the commercial oils showed to contain trans fatty acids although this was not accompanied by the sterene hydrocarbon presence.

scholarly journals Validation and application of a chromatographic method for evaluation of commercial vegetable oils possibly adulterated

2018 ◽  
Vol 22 ◽  
pp. 13
Author(s):  
Katarynna Santos Araújo ◽  
Mariana Oliveira Barbosa ◽  
Carolina Barbosa Malafaia ◽  
Daniella Carla Napoleão
Keyword(s):  
Fatty Acids ◽  
Olive Oil ◽  
Vegetable Oils ◽  
Fatty Acid Methyl Esters ◽  
Unsaturated Fatty Acids ◽  
Methyl Esters ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Acid Methyl ◽  
Two Samples

A method of separation, identification and quantification of fatty acid methyl esters (FAMEs) was developed by gas chromatography with flame ionization detector (GC-FID) using a basic transesterification. In this sense, there were analyzed FAMEs in commercial samples of vegetable oils from soybean and olive oil. The referred method was linear (r>0.99), accurate and precise for palmitic (C16:0), linoleic (C18:2), oleic (C18:2), linolenic (C18:3) and stearic (C18:0) acids. The limits of detection (LOD) and quantification (LOQ) were from 0.03 to 0.31 and 0.08 to 0.94 mg.mL-1 for the five fatty acids, respectively. The results demonstrated that the unsaturated fatty acids were the most abundant for the two samples, being the oleic acid (C18:1) the major in three brands of olive oil (D, E and F), and the linoleic acid (C18:2) the most abundant in soybean oil and the other brands of olive oil (G, H and I), suggesting a possible adulteration in these brands. The proposed method could be considered a tool for the investigation of adulteration in commercial vegetable oils for guaranteed reliability in the results to be comparable with correlated legislations.

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