Bactericidal action of carbon dioxide laser radiation in experimental dental root canals

1986 ◽  
Vol 32 (12) ◽  
pp. 942-946 ◽  
Author(s):  
Kenneth L. Zakariasen ◽  
Douglas N. Dederich ◽  
John Tulip ◽  
Sandra DeCoste ◽  
Susan E. Jensen ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Laser Radiation ◽  
Root Canal ◽  
Carbon Dioxide Laser ◽  
Complete Recovery ◽  
Oral Bacteria ◽  
Human Teeth ◽  
Root Canals ◽  
Laser Alignment ◽  
Major Factors

The ability of a carbon dioxide laser to sterilize the root canal of human teeth has been investigated. Three oral bacteria, Streptococcus sanguis, Streptococcus mutans, and Actinomyces viscosus, and three other bacteria, Bacillus cereus, Staphyloccus aureus, and Pseudomonas aeruginosa were used as experimental organisms. Exposure of cells on glass slides to laser radiation showed there was little difference in the exposure required to kill these six organisms. Complete recovery of bacteria from the root canal was initially a problem and was only achieved when bacterial manipulations and removal were carried out in rapid succession, within 5 min of inoculation. However, the geometry of the instrumented canal and the laser alignment were major factors in achieving consistent cell death of oral bacteria in the root canals. Using sets of 10 teeth, four repeated exposures of 10 W for 1 s was found to sterilize 4 or more of the teeth.

Comparative bactericidai exposures for selected oral bacteria using carbon dioxide laser radiation

1990 ◽  
Vol 10 (6) ◽  
pp. 591-594 ◽  
Author(s):  
Douglas N. Dederich ◽  
Michael A. Pickard ◽  
Allison S. Vaughn ◽  
John Tulip ◽  
Kenneth L. Zakariasen
Keyword(s):  
Carbon Dioxide ◽  
Laser Radiation ◽  
Carbon Dioxide Laser ◽  
Oral Bacteria

Conversion of carbon dioxide laser radiation into visible light in proustite

1975 ◽  
Vol 4 (8) ◽  
pp. 963-965 ◽  
Author(s):  
E N Antonov ◽  
V R Mironenko ◽  
D N Nikogosyan ◽  
M I Glovei
Keyword(s):  
Carbon Dioxide ◽  
Laser Radiation ◽  
Visible Light ◽  
Carbon Dioxide Laser

scholarly journals The effect of different irrigation techniques on the quality of cleaning of root canal walls

2008 ◽  
Vol 55 (4) ◽  
pp. 221-228
Author(s):  
Violeta Pavlovic ◽  
Slavoljub Zivkovic
Keyword(s):  
Root Canal ◽  
Smear Layer ◽  
Sodium Hypochlorite Solution ◽  
Efficient Removal ◽  
Human Teeth ◽  
Root Canals ◽  
Group I ◽  
Different Types ◽  
The Difference

Aim: To evaluate the effect of irrigation techniques, i.e. different types of irrigation needles, on the quality of cleaning of root canal walls using scanning electron microscopy (SEM). Material and Method: The study was conducted on 16 extracted, single-rooted, human teeth. The samples were allocated to two experimental groups depending on the type of the irrigation needle. Conventional needles were used for the irrigation of Group I and laterally perforated ones for Group II. All root canals were instrumented using K files and the Step-back technique. During instrumentation, all samples were irrigated with 2.5 % sodium hypochlorite solution followed by final irrigation with 17 % EDTA for 1 min. After that, all samples were irrigated with 10 ml of distilled water. The roots were, then, sectioned longitudinally and middle one third of each root canal was analyzed using SEM. Quantitative analysis was based on criteria by H?lsmann et al. Results: The obtained results showed that the more efficient removal of debris and the smear layer was accomplished in the group of samples irrigated using laterally perforated needles than conventional needles. The difference between the investigated irrigation techniques were statistically significant (p<0.01). Conclusion: The use of laterally perforated needles for irrigation allows more efficient cleaning of root canal walls, i.e. the removal of debris and the smear layer.

scholarly journals Comparative evaluation of shaping ability of trunatomy and protaper gold files in curved canals using cone?beam computed tomography: An invitro study

2021 ◽  
Vol 6 (2) ◽  
pp. 101-105
Author(s):  
Kadam Krutika Kiran ◽  
Vagarali Hemant ◽  
Pujar Madhu A ◽  
Tamase Aishwarya S ◽  
Sahana Umesh
Keyword(s):  
Computed Tomography ◽  
Cone Beam Computed Tomography ◽  
Root Canal ◽  
Cone Beam ◽  
Human Teeth ◽  
Root Canals ◽  
Before And After ◽  
Canal Transportation ◽  
Group 2 ◽  
Shaping Ability

This study aimed to compare the canal transportation and canal centering ability in the preparation of curved root canals after instrumentation with TruNatomy (TN) (TN; Dentsply Sirona, Maillefer, Ballaigues, Switzerland) and ProTaper Gold (PG) (PG; Dentsply, Tulsa Dental Specialties, Tulsa, OK, USA) files using cone‑beam computed tomography (CBCT). 30 Single rooted extracted human teeth with root curvature ranging from 20-30° according to Schneider’s method were selected. Teeth with any visible cracks or fractures, calcifications, previous root canal treatments were excluded. The teeth were randomly assigned into two groups i.e. Group 1-TN and Group 2-PG (n = 15 each). The teeth were instrumented according to manufacturer’s guidelines for both the groups. Canals were scanned using a CBCT scanner before and after preparation to evaluate the transportation and centering ratio at 3 mm, 5 mm, and 7 mm from the apex. The data analysis was done using SPSS software and the test used was independent sample t test for comparison between the 2 groups.Data obtained suggested that TN group presented lesser canal transportation at the middle third of the root. The PG group showed better centering abitily at apical third of the root canal when both the groups were compared. TN resulted in less transportation than PG at the middle third, and PG showed better centering ability at the apical third. Overall, both systems safely prepared root canals, causing minimal errors.

Effects of Carbon Dioxide Laser Radiation on Bone: An Initial Report

1981 ◽  
Vol 146 (2) ◽  
pp. 120-123 ◽  
Author(s):  
Gary W. Allen ◽  
James C. Adrian
Keyword(s):  
Carbon Dioxide ◽  
Laser Radiation ◽  
Carbon Dioxide Laser ◽  
Initial Report

scholarly journals Prevalence, virulence and antifungal activity of C. albicans isolated from infected root canals

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Sheela B. Abraham ◽  
Farah al Marzooq ◽  
Wan Harun Himratul-Aznita ◽  
Hany Mohamed Aly Ahmed ◽  
Lakshman Perera Samaranayake
Keyword(s):  
Root Canal ◽  
Antifungal Susceptibility ◽  
Production Capacity ◽  
Human Teeth ◽  
Root Canals ◽  
Infected Root ◽  
Dental Hospital ◽  
Canal Systems ◽  
Infected Root Canal ◽  
Genotype A

Abstract Background There is limited data on the prevalence of Candida species in infected root canal systems of human teeth. We attempted to investigate the prevalence, genotype, virulence and the antifungal susceptibility of Candida albicans isolated from infected root canals of patients with primary and post-treatment infections in a UAE population. Methods Microbiological samples from 71 subjects with infected root canals were aseptically collected, and cultured on Sabouraud dextrose agar, and C. albicans was identified using multiplex polymerase chain reaction, and the isolates were further subtyped using ABC genotyping system. Their relative virulence was compared using further four archival samples of endodontic origin from another geographical region, and four more salivary isolates, as controls. The virulence attributes compared were biofilm formation, and production of phospholipase and haemolysin, and the susceptibility to nystatin, amphotericin B, ketoconazole, and fluoconazole was also tested. Results 4 out of 71 samples (5.6%) yielded Candida species. On analysis of variance among the groups, the intracanal isolates, mainly Genotype A, possessed a high degree of phospholipase and haemolysin activity (p < 0.05). The UAE and Finland isolates were stronger biofilm formers and had superior phospholipase production capacity compared with the salivary isolates. All isolates were sensitive to the antifungal chemicals used. The salivary isolates were more sensitive to fluoconazole compared to other groups (p < 0.05). Conclusion The prevalence of C. albicans in infected root canals of patients attending a dental hospital in UAE is low. The strains isolated were good biofilm formers, possessed strong phospholipase and haemolysin activity and were mostly of the genotype A. The fact that the root canal isolates possessed significant hydrolase activity, imply that they are equipped with virulence attributes necessary for survival within a harsh intracanal ecosystem.

scholarly journals Effect of a Surfactant on the Antimicrobial Activity of Sodium Hypochlorite Solutions

2014 ◽  
Vol 25 (5) ◽  
pp. 416-419 ◽  
Author(s):  
Marcos Rodolfo Bolfoni ◽  
Marcelo dos Santos Ferla ◽  
Otávio da Silva Sposito ◽  
Luciano Giardino ◽  
Rogério de Castilho Jacinto ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Root Canal Preparation ◽  
Human Teeth ◽  
Significance Level ◽  
Root Canals ◽  
Tukey Test ◽  
Irrigation Solution ◽  
Period Data

The objective of the present study was to evaluate the antimicrobial activity of sodium hypochlorite (NaOCl) associated with a surfactant. Seventy single-rooted extracted human teeth were inoculated with Enterococcus faecalis, and incubated for 21 days (37 °C). The groups were distributed according to the irrigation solution used during root canal preparation: 5%, 2.5% and 1% NaOCl; 5%, 2.5% and 1% Hypoclean(r), a solution containing a surfactant (cetrimide) associated with NaOCl. Three microbiological samples were collected from each tooth: S1 - before instrumentation; S2 - immediately after instrumentation; and S3 - after a seven-day period. Data were submitted to ANOVA and Tukey test with 5% significance level. The results showed that immediately after root canal preparation (S2), E. faecalis was eliminated in all the experimental groups. However, after 7 days (S3), only the groups in which Hypoclean was used, remained contamination-free, including Hypoclean associated with 1% NaOCl, while the root canals irrigated with 1% NaOCl only, presented the highest percentage of bacterial growth. In conclusion, the addition of surfactant increased the antimicrobial activity of 1% NaOCl to levels similar to 5% NaOCl.

Interaction of Carbon Dioxide Laser Radiation with Enamel and Dentin

1968 ◽  
Vol 47 (2) ◽  
pp. 311-317 ◽  
Author(s):  
Ralph R. Lobene ◽  
B. Raj Bhussry ◽  
Samuel Fine
Keyword(s):  
Carbon Dioxide ◽  
Laser Radiation ◽  
Carbon Dioxide Laser
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