Isolation, characterization, and inoculation of N2-fixing bacteria from dicotyledonous plants

1986 ◽  
Vol 32 (12) ◽  
pp. 912-916 ◽  
Author(s):  
S. Garvin ◽  
W. C. Lindemann
Keyword(s):  
New Mexico ◽  
Acetylene Reduction ◽  
Azospirillum Brasilense ◽  
Wild Plants ◽  
Acetylene Reduction Activity ◽  
Generic Level ◽  
Plant Nitrogen ◽  
Reduction Activity ◽  
Dicotyledonous Plants ◽  
Reducing Bacteria

N2-flxing (acetylene-reducing) bacteria were isolated from roots of five species of wild plants in southern New Mexico. Ground cherry (Physalis wrightii) had the highest frequency of roots with N2-fixing bacteria. Acetylene reduction rates of subcultures were comparable with rates exhibited by Azospirillum (300–500 nmol ethylene∙h−1). Three isolates from ground cherry were characterized to the generic level and one to the level of genus and species. Three of these isolates were identified as Enterobacter spp, while the fourth isolate was identified as Azospirillum brasilense. Inoculation of ground cherry with N2-fixing isolates had minimal effects on plant growth, plant nitrogen content, or plant acetylene reduction activity 7 weeks after inoculation. Comparisons between uninoculated and inoculated plants were generally not statistically significant. Significant acetylene reduction activity and high numbers of N2-fixing bacteria occurred only on the roots of plants grown with little or no added nitrogen.

Effect of inoculation of Zea mays with Azospirillum brasilense strains under temperate conditions

1981 ◽  
Vol 27 (9) ◽  
pp. 871-877 ◽  
Author(s):  
G. W. O'Hara ◽  
M. R. Davey ◽  
J. A. Lucas
Keyword(s):  
Zea Mays ◽  
Nitrogen Content ◽  
Acetylene Reduction ◽  
Azospirillum Brasilense ◽  
Dry Weight ◽  
Yield Response ◽  
Acetylene Reduction Activity ◽  
Reduction Activity ◽  
Mutant Strains ◽  
Nitrogen Concentrations

Seven strains of Azospirillum brasilense were compared for their effect on the growth of Zea mays grown under temperate conditions in sand–vermiculite pot cultures. Inoculation with all seven strains tested, including Fix− mutant strains, increased dry weight and total nitrogen content of shoots, but nitrogen concentrations were unaffected. Low and variable rates of acetylene reduction activity were observed from excised roots of inoculated plants without preincubation. Estimates of N2-fixing A. brasilense associated with inoculated roots showed differences between strains in establishing themselves in the rhizosphere and endorhizosphere. In some strains enrichment in the endorhizosphere of roots occurred following inoculation, but the relative numbers and location of the strains did not appear to affect the yield response.

Wheat root colonization and nitrogenase activity by Azospirillum isolates from crop plants in Korea

2005 ◽  
Vol 51 (11) ◽  
pp. 948-956 ◽  
Author(s):  
Chungwoo Kim ◽  
Mihály L Kecskés ◽  
Rosalind J Deaker ◽  
Kate Gilchrist ◽  
Peter B New ◽  
...  
Keyword(s):  
Acetylene Reduction ◽  
Azospirillum Brasilense ◽  
Nitrogenase Activity ◽  
Lateral Roots ◽  
Wheat Root ◽  
Acetylene Reduction Activity ◽  
Galactosidase Activity ◽  
Azospirillum Lipoferum ◽  
Wheat Roots ◽  
Reduction Activity

Nitrogen-fixing bacteria were isolated from the rhizosphere of different crops of Korea. A total of 16 isolates were selected and characterized. Thirteen of the isolates produced characteristics similar to those of the reference strains of Azospirillum, and the remaining 3 isolates were found to be Enterobacter spp. The isolates could be categorized into 3 groups based on their ARDRA patterns, and the first 2 groups comprised Azospirillum brasilense and Azospirillum lipoferum. The acetylene reduction activity (ARA) of these isolates was determined for free cultures and in association with wheat roots. There was no correlation between pure culture and plant-associated nitrogenase activity of the different strains. The isolates that showed higher nitrogenase activities in association with wheat roots in each group were selected and sequenced. Isolates of Azospirillum brasilense CW301, Azospirillum brasilense CW903, and Azospirillum lipoferum CW1503 were selected to study colonization in association with wheat roots. We observed higher expression of β-galactosidase activity in A. brasilense strains than in A. lipoferum strains, which could be attributed to their higher population in association with wheat roots. All strains tested colonized and exhibited the strongest β-galactosidase activity at the sites of lateral roots emergence.Key words: Azospirillum, acetylene reduction activity, 16S rDNA, ARDRA patterns, lacZ fusion.

Nitrogen fixation associated with 'Park' Kentucky bluegrass (Poapratensis L.)

1979 ◽  
Vol 25 (10) ◽  
pp. 1197-1200 ◽  
Author(s):  
R. C. Shearman ◽  
W. L. Pedersen ◽  
R. V. Klucas ◽  
E. J. Kinbacher
Keyword(s):  
Nitrogen Fixation ◽  
Acetylene Reduction ◽  
Poa Pratensis ◽  
Kentucky Bluegrass ◽  
Acetylene Reduction Activity ◽  
Controlled Environment ◽  
Nitrogen Accumulation ◽  
Reduction Activity ◽  
Significant Difference ◽  
Reduction Assay

Associative nitrogen fixation in Kentucky bluegrass (Poa pratensis L.) turfs inoculated with five nitrogen-fixing bacterial isolates was evaluated using the acetylene reduction assay and nitrogen accumulation as indicators of fixation. 'Park' and 'Nugget' Kentucky bluegrass turfs were grown in controlled environment chambers and inoculated with Klebsiella pneumoniae (W-2, W-6, and W-14), Erwinia herbicola (W-8), and Enterobacter cloacae (W-11). 'Park' inoculated with K. pneumoniae (W-6) had significant acetylene reduction activity using undisturbed turfs. Other treatments including turfs treated with heat-killed cells had no significant difference in acetylene reduction. In a second study, 'Park' and 'South Dakota Certified' turfs were grown in a greenhouse and inoculated with K. pneumoniae (W-6) and E. herbicola (W-8). 'Park' inoculated with K. pneumoniae (W-6) had increased acetylene reduction activity rates and also a greater nitrogen accumulation in aerial tissues when compared to controls. Acetylene reduction activity was correlated (r = 0.92) to nitrogen accumulation. Other treatments did not effectively increase acetylene reduction activity or nitrogen accumulation.

scholarly journals Diversity and Effectivity of Indigenous Mesorhizobium Strains for Chickpea (Cicer arietinum L.) in Myanmar

Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 287 ◽  
Author(s):  
Khin Myat Soe ◽  
Aung Zaw Htwe ◽  
Kyi Moe ◽  
Abiko Tomomi ◽  
Takeo Yamakawa
Keyword(s):  
Sequence Analysis ◽  
Cicer Arietinum ◽  
Acetylene Reduction ◽  
Dry Weight ◽  
Acetylene Reduction Activity ◽  
Rrna Gene ◽  
Cicer Arietinum L ◽  
Reduction Activity ◽  
Legume Production ◽  
High Level

Chickpea (Cicer arietinum L.) is one of the world’s main leguminous crops that provide chief source of food for humans. In the present study, we characterized thirty isolates of indigenous chickpea rhizobia from Myanmar based on the sequence analysis of the bacterial 16S rRNA gene. The sequence analysis confirmed that all isolates were categorized and identified as the genus Mesorhizobium and they were conspecific with M. plurifarium, M. muliense, M. tianshanense, and M. sp. This is the first report describing M. muliense, M. tianshanense, and M. plurifurium from different geographical distribution of indigenous mesorhizobia of chickpea in Myanmar. In order to substitute the use of chemical fertilizers in legume production, there is a need for the production of Biofertilizers with rhizobial inoculants. The effectiveness of Myanmar Mesorhizobim strains isolated from soil samples of major chickpea growing areas of Myanmar for plant growth and nitrogen fixation were studied in pot experiments. The nodule dry weight and acetylene reduction activity of the plant inoculated with Mesorhizobium tianshanense SalCP19 was significantly higher than the other tested isolates in Yezin-4 chickpea variety. But, Mesorhizobium sp. SalCP17 was showed high level of acetylene reduction activity per plant in Yezin-6 chickpea variety.

Acetylene reduction activity in salt‐tolerant alfalfa andrhizobium

1989 ◽  
Vol 3 (4) ◽  
pp. 469-476 ◽  
Author(s):  
Ramzi M. Mohammad ◽  
W.F. Campbell ◽  
M.D. Rumbaugh
Keyword(s):  
Acetylene Reduction ◽  
Acetylene Reduction Activity ◽  
Salt Tolerant ◽  
Reduction Activity
Sign in / Sign up

Export Citation Format

Share Document