C-390 as sole selective agent for isolation of Pseudomonas aeruginosa from hospital waste water

1986 ◽  
Vol 32 (6) ◽  
pp. 513-515 ◽  
Author(s):  
A. H. Havelaar ◽  
M. During

Pseudomonas aeruginosa was recovered (in numbers ranging from 102 to 105 colony-forming units per millilitre) from heavily contaminated hospital waste water when grown at 41.5 °C on a differential medium agar containing 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan (C-390) at a final concentration of 30 μg/mL. The medium appeared to be highly selective for P. aeruginosa with 95–100% of all colonies isolated from four different hospital waste waters being identified as P. aeruginosa. Many strains of P. aeruginosa isolated from hospital waste waters failed to hydrolyse casein when grown on skim milk agar and this medium appeared to restrict pigment production to only pyoverdin (detectable only under ultraviolet light). However, most strains were capable of casein hydrolysis when grown on a modified skim milk medium.

2012 ◽  
Vol 82 (1) ◽  
pp. 19-24 ◽  
Author(s):  
A.S. Breathnach ◽  
M.D. Cubbon ◽  
R.N. Karunaharan ◽  
C.F. Pope ◽  
T.D. Planche

Author(s):  
Maria Y. Savostyanova ◽  
◽  
Lidia А. Norina ◽  
Arina V. Nikolaeva ◽  
◽  
...  

Retaining of water resources quality is one of the global ecological problems of the modern time. The most promising direction in solving the problem of water resources protection is the reduction of negative environmental influence of waste water from production facilities by upgrading the existing water treatment technologies. To treat utility water, technical and rain water from site facilities of Transneft system entities, the specialists developed and approved standard technological diagrams, which are used in producing treatment facilities. The standard technological diagrams provide for all necessary stages of waste water treatment ensures the reduction of pollution level to normal values. However, during operation of treatment facilities it was established, that to ensure the required quality of waste water treatment with initially high levels of pollution, the new technological solutions are necessary. The author presents the results of scientific-research work, in the context of which the best affordable technologies were identified in the area of the treatment of waste water with increased content of pollutants and non-uniform ingress pattern. On the basis of the research results the technical solutions were developed for optimization of operation of existing waste water treatment facilities by means of using combined treatment of technical and rain waters and utility waste waters and applying bioreactor with movable bed – biochips. The use of bioreactor with movable bed allows the increase in the area of active surface, which facilitates increase and retention of biomass. Biochips are completely immersed into waste waters, and biofilm is formed on the entire volume of immersion area, facilitating retention of biomass and preventing formation of sediments. Due to mixing the floating device with biofilm constantly moves along the whole area of bioreactor, and, in doing so, speeds up biochemical processes and uniformity of treatment. The advantages of a bioreactor with movable bed – its active sludge durability against increased and changing pollutant concentrations, change of waste water temperature and simplicity of application – ensured the possibility of its use for blending utility waters, technical and rain waters.


2007 ◽  
Vol 2 (1) ◽  
Author(s):  
D.A. Danilovich ◽  
M.N. Kozlov ◽  
V.I. Sklyar ◽  
Yu.A. Nikolaev ◽  
N.M. Shchegolkova ◽  
...  

This work demonstrates a possibility of composting of municipal waste waters sludge (WWS) digested during 5-7 days, of the Kuryanovo waste water treatment plants (the city of Moscow) without the use of traditional organic additives – sawdust, peat, thatch. As a recycling filler (repeatedly used), enhancing the porosity of the composted mixture, ribbed polyethylene spheres (∅=8 cm) and wood chips (having dimension of 5-10 cm) were used. Composting was performed efficiently in both cases, however, the use of wood ships had technological and economical advantages. The process of composting was carried out during 2-4 weeks and was composed of classical phases of active heating (a rate of 0.2-0.3oC/h was obtained), maintaining of maximum temperature during 1-2 days, and cooling down to the ambient temperature. During the course of composting, a product was obtained, having attractive organoleptic properties, suitable for application in municipal laying-out of greenery.


1994 ◽  
Vol 29 (9) ◽  
pp. 179-185 ◽  
Author(s):  
Åke Undén

In a programme started in 1988, the waste water discharges from the major chemical industries in Sweden have been investigated. The primary objectives were to gather information on the major sources of such discharges and to initiate action so as to achieve significant reductions where required. In this paper the investigation programme is presented together with some early conclusions. As could be expected there are no simple common rules for these industries, each effluent should be considered as a separate problem. It was concluded that these waste waters were in most cases more toxic than was acceptable, and that further purifying measures were required in these cases.


1995 ◽  
Vol 31 (5-6) ◽  
pp. 75-79 ◽  
Author(s):  
M. Würzer ◽  
A. Wiedenmann ◽  
K. Botzenhart

In Germany the application of procedures such as flocculation and filtration in the preparation of drinking water results in the annual production of an estimated 500,000 t of sediments and sludges. Some of these residues have a potential for being reused, for example in agriculture, forestry, brickworks or waste water treatment. To assess the microbiological quality of residues from waterworks methods for the detection of enterobacteria, Escherichia coli, Salmonella, Pseudomonas aeruginosa, Legionella, poliovirus, Ascaris suis eggs and Cryptosporidium have been evaluated regarding their detection limits and were applied to various residues from German waterworks. Results show that sediments and sludges may contain pathogenic bacteria, viruses and protista. When residues from waterworks are intended to be reused in agriculture or forestry the microbiological quality should therefore be considered.


2012 ◽  
Vol 32 (1) ◽  
pp. 142-150 ◽  
Author(s):  
Danila Soares Caixeta ◽  
Thiago Henrique Scarpa ◽  
Danilo Florisvaldo Brugnera ◽  
Dieyckson Osvani Freire ◽  
Eduardo Alves ◽  
...  

The biofilm formation of Pseudomonas aeruginosa and Pseudomonas fluorescens on AISI 304 stainless steel in the presence of reconstituted skim milk under different temperatures was conducted, and the potential of three chemical sanitizers in removing the mono-species biofilms formed was compared. Pseudomonas aeruginosa cultivated in skim milk at 28 °C presented better growth rate (10.4 log CFU.mL-1) when compared with 3.7 and 4.2 log CFU.mL-1 for P. aeruginosa and P. fluorescens cultivated at 7 °C, respectively. Pseudomonas aeruginosa formed biofilm when cultivated at 28 °C. However, only the adhesion of P. aeruginosa and P. fluorescens was observed when incubated at 7 °C. The sodium dichloroisocyanurate was the most efficient sanitizer in the reduction of the adhered P. aeruginosa cells at 7 and 28 °C and those on the biofilm, respectively. The hydrogen peroxide was more effective in the reduction of adhered cells of P. fluorescens at 7 °C.


2000 ◽  
Vol 18 (No. 5) ◽  
pp. 170-174
Author(s):  
P. Navrátilová

Waste waters from dairies were tested for the presence of bacterial pathogens – Listeria monocytogenes, Staphylococcus aureus and Salmonella spp. The prevalence of bacteria was investigated in each stage of the cleaning process (activated sludges systems) too. Two hunder samples of raw waste water, activated sludge, returned activated sludge, excess sludge and treated water from 14 dairies were tested. The samples were all negative for Salmonella spp. From a total of 102 (51%) strains Listeria spp., Listeria inoccua 95 (47.5%) and Listeria monocytogenes 7 (3.5%) were identified. 47 samples were positive for S. aureus. L. monocytogenes were detected in raw waste water 1 (1.6%), in activated sludge 3 (5.5%), in excess sludge 1 and in treated water 2 (3.1%). S. aureus were detected in raw waste water 14 (22.6%), in activated sludge 23 (41.8%), in excess sludge 1 and in treated water 8 (12.3%). These results demonstrate a prevalence of L. monocytogenes and S. aureus in waste waters from dairies. During the cleaning process pathogenic bacteria were not devitalized. The excess sludge and treated water including pathogenic micro-organisms represent a potential health hazard.


2009 ◽  
Vol 15 (1) ◽  
pp. 53-56
Author(s):  
M. Vukcevic ◽  
D. Blecic ◽  
Z. Blecic ◽  
D. Cabarkapa

The new concept of Cleaner Production (CP) was recently introduced in the Factory for the production of welding electrodes (FEP) in Pluzine, by following some activities, raw materials and products. By the identification of some sub-processes with CP opportunities, focus points were identified and subjected to some technical modifications and innovations. The most significant results were obtained regarding waste waters, through the modifications of existing waste water generation and a possible switch to an other technology, the reduction of K-silica waste through a technological modification, a system of powder transport, as well as the production of coated electrodes.


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