Restriction-modification systems in Streptomyces antibioticus

1985 ◽  
Vol 31 (10) ◽  
pp. 942-946 ◽  
Author(s):  
Jesùs Sànchez ◽  
Covadonga Barbès ◽  
Angeles Hernandez ◽  
Clara-Rosa G. de los Reyes Gavilàn ◽  
Carlos Hardisson
Keyword(s):  
Biological Indicators ◽  
Modification System ◽  
Streptomyces Antibioticus ◽  
Restriction Modification System ◽  
Different Strains ◽  
Restriction Modification ◽  
Restriction Modification Systems

Several restriction systems were detected in different strains of Streptomyces antibioticus by using actinophages as biological indicators. Adsorption of phages to the bacteria, together with the study of the efficiency of plating gave an initial indication of restriction in three strains. The alternation of efficiency of plating values obtained from restricting and nonrestricting hosts, gave evidence for the presence of a restriction-modification system in another strain. No common modification systems were detected among the different strains tested. Two specific endonucleases with a possible role in restriction were detected in strains ATCC 11891 and ETH 7451, respectively.

scholarly journals Restriction-modification systems and DNA methylation profile of Pectobacterium carotovorum 2A

2021 ◽  
pp. 71-77
Author(s):  
Yulia V. Diubo ◽  
Artur E. Akhremchuk ◽  
Leonid N. Valentovich ◽  
Yevgeny A. Nikolaichik
Keyword(s):  
Methylation Profile ◽  
Type I ◽  
Pectobacterium Carotovorum ◽  
Modification System ◽  
Restriction Modification System ◽  
Type Iv ◽  
Oxford Nanopore ◽  
Dna Methylation Profile ◽  
Restriction Modification ◽  
Restriction Modification Systems

The methylation profile of Pectobacterium carotovorum 2A genome was studied using the Oxford Nanopore sequencing technology. The specificity of the methylase subunits of the three restriction-modification systems of this strain was determined. Analysis of homologous systems showed the uniqueness of the type I restriction-modification system and the type IV restriction system specific to methylated DNA of this strain. The work confirms the applicability of Oxford Nanopore technology to the analysis of bacterial DNA modifications and is also the first example of such an analysis for Pectobacterium spp.

scholarly journals Effects of mutations in phage restriction sites during escape from restriction–modification

2017 ◽  
Vol 13 (12) ◽  
pp. 20170646 ◽  
Author(s):  
Maroš Pleška ◽  
Călin C. Guet
Keyword(s):  
Restriction Site ◽  
Response To Selection ◽  
Modification System ◽  
Restriction Modification System ◽  
Restriction Sites ◽  
Restriction Modification ◽  
Insight Into ◽  
Restriction Modification Systems ◽  
Site Avoidance

Restriction–modification systems are widespread genetic elements that protect bacteria from bacteriophage infections by recognizing and cleaving heterologous DNA at short, well-defined sequences called restriction sites. Bioinformatic evidence shows that restriction sites are significantly underrepresented in bacteriophage genomes, presumably because bacteriophages with fewer restriction sites are more likely to escape cleavage by restriction–modification systems. However, how mutations in restriction sites affect the likelihood of bacteriophage escape is unknown. Using the bacteriophage λ and the restriction–modification system EcoRI, we show that while mutation effects at different restriction sites are unequal, they are independent. As a result, the probability of bacteriophage escape increases with each mutated restriction site. Our results experimentally support the role of restriction site avoidance as a response to selection imposed by restriction–modification systems and offer an insight into the events underlying the process of bacteriophage escape.

scholarly journals Mobility of a Restriction-Modification System Revealed by Its Genetic Contexts in Three Hosts

2002 ◽  
Vol 184 (9) ◽  
pp. 2411-2419 ◽  
Author(s):  
Marc Naderer ◽  
Jessica R. Brust ◽  
Dieter Knowle ◽  
Robert M. Blumenthal
Keyword(s):  
Open Reading Frames ◽  
Modification System ◽  
Integrase Gene ◽  
Sequence Characterization ◽  
Restriction Modification System ◽  
Subunit Interface ◽  
Bacteriophage P4 ◽  
Flanking Sequences ◽  
Restriction Modification ◽  
Restriction Modification Systems

ABSTRACT The flow of genes among prokaryotes plays a fundamental role in shaping bacterial evolution, and restriction-modification systems can modulate this flow. However, relatively little is known about the distribution and movement of restriction-modification systems themselves. We have isolated and characterized the genes for restriction-modification systems from two species of Salmonella, S. enterica serovar Paratyphi A and S. enterica serovar Bareilly. Both systems are closely related to the PvuII restriction-modification system and share its target specificity. In the case of S. enterica serovar Paratyphi A, the restriction endonuclease is inactive, apparently due to a mutation in the subunit interface region. Unlike the chromosomally located Salmonella systems, the PvuII system is plasmid borne. We have completed the sequence characterization of the PvuII plasmid pPvu1, originally from Proteus vulgaris, making this the first completely sequenced plasmid from the genus Proteus. Despite the pronounced similarity of the three restriction-modification systems, the flanking sequences in Proteus and Salmonella are completely different. The SptAI and SbaI genes lie between an equivalent pair of bacteriophage P4-related open reading frames, one of which is a putative integrase gene, while the PvuII genes are adjacent to a mob operon and a XerCD recombination (cer) site.

Cloning the KpnI restriction-modification system in Escherichia coli

Gene ◽  
1991 ◽  
Vol 97 (1) ◽  
pp. 97-102 ◽  
Author(s):  
Alan W. Hammond ◽  
Gary F. Gerard ◽  
Deb K. Chatterjee
Keyword(s):  
Escherichia Coli ◽  
Modification System ◽  
Restriction Modification System ◽  
Restriction Modification

scholarly journals A putative mobile genetic element carrying a novel type IIF restriction-modification system (PluTI)

2010 ◽  
Vol 38 (9) ◽  
pp. 3019-3030 ◽  
Author(s):  
Feroz Khan ◽  
Yoshikazu Furuta ◽  
Mikihiko Kawai ◽  
Katarzyna H. Kaminska ◽  
Ken Ishikawa ◽  
...  
Keyword(s):  
Mobile Genetic Element ◽  
Genetic Element ◽  
Modification System ◽  
Restriction Modification System ◽  
Restriction Modification

scholarly journals Complete Genome Sequence of Brevibacterium linens SMQ-1335

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Alessandra G. de Melo ◽  
Simon J. Labrie ◽  
Jeannot Dumaresq ◽  
Richard J. Roberts ◽  
Denise M. Tremblay ◽  
...  
Keyword(s):  
Complete Genome Sequence ◽  
Open Reading Frames ◽  
Type I ◽  
Industrial Strain ◽  
Modification System ◽  
Brevibacterium Linens ◽  
Restriction Modification System ◽  
New Type ◽  
Restriction Modification ◽  
Reading Frames

Brevibacterium linens is one of the main bacteria found in the smear of surface-ripened cheeses. The genome of the industrial strain SMQ-1335 was sequenced using PacBio. It has 4,209,935 bp, a 62.6% G+C content, 3,848 open reading frames, and 61 structural RNAs. A new type I restriction-modification system was identified.

Sign in / Sign up

Export Citation Format

Share Document