Effect of carbon dioxide on growth of Pseudomonas putida ATCC 11172 on asparagine, citrate, glucose, and lactate in batch and continuous culture

1985 ◽  
Vol 31 (9) ◽  
pp. 763-766 ◽  
Author(s):  
Göran Molin
Keyword(s):  
Carbon Dioxide ◽  
Growth Rate ◽  
Pseudomonas Putida ◽  
Continuous Culture ◽  
Specific Growth ◽  
Carbon Sources ◽  
Maximum Specific Growth Rate ◽  
The Other ◽  
Continuous Cultures ◽  
Batch Cultures

The growth of Pseudomonas putida ATCC 11172 on L-asparagine, citrate, D-glucose, and L-lactate was followed in air and in 40% CO2 + air, using batch and carbon-limited continuous cultures. Batch cultures in air utilized a mixture of the carbon sources simultaneously. However, a change to 40% CO2 favoured the utilization of glucose. The maximum specific growth rate (μmax) in air was about 0.3 h−1 on glucose and 0.6 h−1 on the other carbon sources. In CO2, the μmax for glucose was reduced by 16% compared with almost 60–70% for the others. An order of preference for the different carbon sources in continuous cultures was determined by comparing the dilution rates at which the different carbon sources started to appear in the effluent. Glucose was the first compound to appear as the dilution rate increased (lowest preference when grown in air). In 40% CO2, the μmax for glucose was slightly higher than the others and the recorded preference for glucose in continuous culture was equal to that for citrate but was somewhat lower than that of lactate and asparagine. D-Gluconate and glucono-δ-lactone were produced as a step in the utilization of glucose. The D-gluconate production was enhanced by CO2.

Evolution of Escherichia coli During Growth in a Constant Environment

Genetics ◽  
1987 ◽  
Vol 116 (3) ◽  
pp. 349-358
Author(s):  
Robert B Helling ◽  
Christopher N Vargas ◽  
Julian Adams
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Specific Growth Rate ◽  
Continuous Culture ◽  
Specific Growth ◽  
Maximum Specific Growth Rate ◽  
Constant Environment ◽  
Single Clone ◽  
Mixed Populations

ABSTRACT Populations of Escherichia coli, initiated with a single clone and maintained for long periods in glucose-limited continuous culture, developed extensive polymorphisms. In one population, examined after 765 generations, two majority and two minority types were identified. Stable mixed populations were reestablished from the isolated strains. Factors involved in the development of this polymorphism included differences in the maximum specific growth rate and in the transport of glucose, and excretion of metabolites by some clones which were utilized by minority clones.

scholarly journals Elucidating the competition between heterotrophic denitrification and DNRA using the resource-ratio theory

2019 ◽  
Author(s):  
Mingsheng Jia ◽  
Mari K.H. Winkler ◽  
Eveline I.P. Volcke
Keyword(s):  
Growth Rate ◽  
Organic Carbon ◽  
Dilution Rate ◽  
Nitrate Reduction ◽  
Specific Growth ◽  
Nitrogen Loss ◽  
Maximum Specific Growth Rate ◽  
Comprehensive Understanding ◽  
Continuous Cultures ◽  
Heterotrophic Denitrification

AbstractDenitrification and dissimilatory nitrate reduction to ammonium (DNRA) are two microbial processes competing for nitrate and organic carbon (COD). Their competition has great implications for nitrogen loss, conservation, and greenhouse gas emissions. Nevertheless, a comprehensive and mechanistic understanding of the governing factors for this competition is still lacking. We applied the resource-ratio theory and verified it with competition experiments of denitrification and DNRA reported in the literature. Based on this theory, we revealed how COD/N ratio, influent resource concentrations, dilution rate, and stoichiometric and kinetic parameters individually and collectively define the boundaries for different competition outcomes in continuous cultures. The influent COD/N ratio alone did not drive competition outcome as the boundary COD/N ratio for different competition outcomes changed significantly with influent resource concentrations. The stoichiometry of the two processes was determinative for the boundaries, whereas the affinity for the resources (Ks), maximum specific growth rate (μmax) of the two species and the dilution rate had significant impacts as well but mainly at low influent resource concentrations (e.g., <100 μM nitrate). The proposed approach allows for a more comprehensive understanding of the parameters controlling microbial selection and explains apparently conflicting experimental results. The results from this model also provide testable hypotheses and tools for understanding and managing the fate of nitrate in ecosystems and for other species that compete for two resources.

The excretion of organic nitrogen by marine algae in batch and continuous culture

1972 ◽  
Vol 50 (12) ◽  
pp. 2605-2611 ◽  
Author(s):  
B. S. Newell ◽  
G. Dalpont ◽  
B. R. Grant
Keyword(s):  
Carbon Dioxide ◽  
Growth Rate ◽  
Continuous Culture ◽  
Organic Material ◽  
Dissolved Organic Nitrogen ◽  
Organic Nitrogen ◽  
Marine Algae ◽  
Algal Species ◽  
Continuous Cultures

Production of varying amounts of dissolved organic nitrogen has been demonstrated in cultures of eight species of marine algae. The material appears to be polypeptide. Continuous culture experiments with two algal species showed that production of this organic material was not increased by stress in the form of changes in growth rate, light, carbon dioxide, or pH. It was increased when the continuous cultures were allowed to stagnate.

scholarly journals Influence of Carbon Source on Nitrate Removal by Nitrate-TolerantKlebsiella oxytoca CECT 4460 in Batch and Chemostat Cultures

1998 ◽  
Vol 64 (8) ◽  
pp. 2970-2976 ◽  
Author(s):  
Guadalupe Piñar ◽  
Karin Kovárová ◽  
Thomas Egli ◽  
Juan L. Ramos
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Specific Growth ◽  
Nitrate Removal ◽  
Dry Weight ◽  
Specific Rate ◽  
Continuous Cultures ◽  
Batch Cultures ◽  
Chemostat Cultures ◽  
C And N

ABSTRACT The nitrate-tolerant organism Klebsiella oxytoca CECT 4460 tolerates nitrate at concentrations up to 1 M and is used to treat wastewater with high nitrate loads in industrial wastewater treatment plants. We studied the influence of the C source (glycerol or sucrose or both) on the growth rate and the efficiency of nitrate removal under laboratory conditions. With sucrose as the sole C source the maximum specific growth rate was 0.3 h−1, whereas with glycerol it was 0.45 h−1. In batch cultures K. oxytocacells grown on sucrose or glycerol were able to immediately use sucrose as a sole C source, suggesting that sucrose uptake and metabolism were constitutive. In contrast, glycerol uptake occurred preferentially in glycerol-grown cells. Independent of the preculture conditions, when sucrose and glycerol were added simultaneously to batch cultures, the sucrose was used first, and once the supply of sucrose was exhausted, the glycerol was consumed. Utilization of nitrate as an N source occurred without nitrite or ammonium accumulation when glycerol was used, but nitrite accumulated when sucrose was used. In chemostat cultures K. oxytoca CECT 4460 efficiently removed nitrate without accumulation of nitrate or ammonium when sucrose, glycerol, or mixtures of these two C sources were used. The growth yields and the efficiencies of C and N utilization were determined at different growth rates in chemostat cultures. Regardless of the C source, yield carbon (YC) ranged between 1.3 and 1.0 g (dry weight) per g of sucrose C or glycerol C consumed. Regardless of the specific growth rate and the C source, yield nitrogen (YN) ranged from 17.2 to 12.5 g (dry weight) per g of nitrate N consumed. In contrast to batch cultures, in continuous cultures glycerol and sucrose were utilized simultaneously, although the specific rate of sucrose consumption was higher than the specific rate of glycerol consumption. In continuous cultures double-nutrient-limited growth appeared with respect to the C/N ratio of the feed medium and the dilution rate, so that for a C/N ratio between 10 and 30 and a growth rate of 0.1 h−1 the process led to simultaneous and efficient removal of the C and N sources used. At a growth rate of 0.2 h−1the zone of double limitation was between 8 and 11. This suggests that the regimen of double limitation is influenced by the C/N ratio and the growth rate. The results of these experiments were validated by pulse assays.

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