Observations préliminaires sur l'accumulation d'hydrocarbures chez Candida steatolytica: relation avec la synthèse des lipides

1985 ◽  
Vol 31 (7) ◽  
pp. 620-624 ◽  
Author(s):  
M. Elisabetta Guerzoni ◽  
Paolo Lambertini ◽  
Agostino Cavazza ◽  
Rosa Marchetti
Keyword(s):  
Carbon Source ◽  
Lipid Composition ◽  
Lipid Accumulation ◽  
Nitrogen Limitation ◽  
Culture Medium ◽  
Lipomyces Starkeyi ◽  
Cell Lipid ◽  
Branched Hydrocarbons ◽  
Lipid Fractions

Different cell lipid accumulation and composition patterns were observed in the yeasts Lipomyces starkeyi and Candida steatolytica by varying the nutritive conditions. In Lipomyces starkeyi 303 which accumulates high levels of lipids as triglycerides, the composition of the different lipid fractions was relatively unaffected by the culture medium. In contrast, the lipid composition of Candida steatolytica 20C was strongly affected by the composition of the medium under conditions of nitrogen limitation and when starch was used as the carbon source, high levels of straight and branched hydrocarbons were observed. [Traduction de l'auteur]

Research on microbial lipid production from potato starch wastewater as culture medium by Lipomyces starkeyi

2013 ◽  
Vol 67 (8) ◽  
pp. 1802-1808 ◽  
Author(s):  
Jun-Xian Liu ◽  
Qin-Yan Yue ◽  
Bao-Yu Gao ◽  
Yan Wang ◽  
Qian Li ◽  
...  
Keyword(s):  
Cell Growth ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Culture Medium ◽  
Potato Starch ◽  
Inoculum Size ◽  
Microbial Lipid ◽  
Lipomyces Starkeyi ◽  
Starch Wastewater ◽  
Source Concentration

In this paper, potato starch wastewater as culture medium was treated by the oleaginous yeast Lipomyces starkeyi to biosynthesize microbial lipid. The result indicated that carbon source types, carbon source concentration, nitrogen source types, nitrogen source concentration, inoculum size, and cultivation time all had a significant effect on cell growth and microbial lipid accumulation in batch cultures. A measure of 120 g/L of glucose concentration, 3.0 g/L of (NH4)2SO4 concentration, 10% inoculum size, and incubation time 96 h cultivated in a shaking flask at 30 °C were found to be the optimal conditions not only for cell growth but also for lipid synthesis. Under this condition, the cellular biomass and lipid content could reach 2.59 g/L and 8.88%, respectively. This work provides a new method for effective utilization of potato starch wastewater, which has particular social and economic benefits for yeast treatment technology.

Effect of Carbon Sources and Fe3+ on the Growth and Lipid Accumulation of Monoraphidium sp. FXY-10 under Mixotrophic Culture Condition

2014 ◽  
Vol 1070-1072 ◽  
pp. 157-163
Author(s):  
Hao Miao Jiangwang ◽  
Li Huang ◽  
Xu Ya Yu
Keyword(s):  
Carbon Source ◽  
Culture Condition ◽  
Lipid Accumulation ◽  
Sodium Acetate ◽  
Culture Medium ◽  
Carbon Sources ◽  
Biomass Productivity ◽  
Lipid Productivity ◽  
Mixotrophic Culture ◽  
Final Cell Density

Effects of different carbon source and Fe3+ for the growth and lipid accumulation of Monoraphidium sp. FXY-10 cultured mixotrophically was studied in our present work. The final cell density was reached to 2.626 g L-1 when glucose was the only carbon source, which is 1.43-fold to sodium acetate (1.834 g L-1), far higher than sucrose (0.251 g L-1) and xylitol (0.471 g L-1), but barely grow in other culture condition. Additionally, the highest algae lipid productivity (77.45 mg L-1 d-1) was obtained in 10 g L-1 glucose group, which indicated that glucose was the optimal carbon source for growth and lipid accumulation of Monoraphidium sp. FXY-10. Nevertheless, Monoraphidium sp. FXY-10 was grew slowly in BG-11 culture medium with the absence of Fe3+. The biomass was achieved at the top with 50μM Fe3+ added. With the increase of Fe3+ concentration, it showed no variation in the growth of microalgae. The highest biomass productivity (209.87 mg L-1 d-1) was reached when the Fe3+ concentration was at 150μM while highest lipid productivity (94.05 mg L-1 d-1) reached at 50μM, which indicated that Fe3+ was one of the most indispensable trace elements for the growth and lipid accumulation of Monoraphidium sp. FXY-10.

scholarly journals Nitrogen limitation, oxygen limitation, and lipid accumulation in Lipomyces starkeyi

2016 ◽  
Vol 200 ◽  
pp. 780-788 ◽  
Author(s):  
Christopher H. Calvey ◽  
Yi-Kai Su ◽  
Laura B. Willis ◽  
McSean McGee ◽  
Thomas W. Jeffries
Keyword(s):  
Lipid Accumulation ◽  
Nitrogen Limitation ◽  
Oxygen Limitation ◽  
Lipomyces Starkeyi

scholarly journals Influence of cultivation conditions on IAA - producing activity of endophytic bacterial strains isolated from morning-glory (IPOMOEA PES-CAPRAE (L.) R.Br.)

2021 ◽  
Vol 36 ◽  
pp. 05003
Author(s):  
Nguyen Van Zhang ◽  
Nguyen Thi Thu ◽  
Vu Thi Linh ◽  
V.V. Pylnev ◽  
M.I. Popchenko
Keyword(s):  
Carbon Source ◽  
Culture Medium ◽  
Nitrogen Sources ◽  
Morning Glory ◽  
Bacterial Strains ◽  
Cultivation Conditions ◽  
Bacillus Mycoides ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Study Results

This work presents the experimental study results of the influence of the culture medium on the ability to IAA synthesis of three endophytic strains TH10R, TH11T, and TH13T from roots of Ipomoea pes-caprae. Three investigated strains give the highest IAA concentration after 96 h of cultivation. A significant increase in IAA biosynthesis was obtained by cultivating the TH10R strain in a medium containing lactose or starch as a carbon source and NH4Cl or KNO3 as a nitrogen source. The TH11T strain produces the maximum amount of IAA, using glucose or xylose and KNO3 or NH4NO3 as carbon and nitrogen sources, respectively. Sucrose is a suitable carbon source for the TH13T strain; on a sucrose-containing medium, the TH13T strain produces the highest IAA amount. The most active strain is TH10R, identified as Bacillus mycoides and named Bacillus mycoides TH10R.

scholarly journals Lipid accumulation capability of typical non‐acclimated activated sludge microbial consortia using methane gas as secondary carbon source

2020 ◽  
Vol 2 (4) ◽  
Author(s):  
Dhan Lord B. Fortela ◽  
Wayne Sharp ◽  
Emmanuel Revellame ◽  
Andrei Chistoserdov ◽  
William Holmes ◽  
...  
Keyword(s):  
Carbon Source ◽  
Activated Sludge ◽  
Lipid Accumulation ◽  
Microbial Consortia ◽  
Methane Gas ◽  
Acclimated Activated Sludge ◽  
Secondary Carbon

The isocitrate dehydrogenase gene of oleaginous yeast Lipomyces starkeyi is linked to lipid accumulation

2009 ◽  
Vol 55 (9) ◽  
pp. 1062-1069 ◽  
Author(s):  
Wei Tang ◽  
Sufang Zhang ◽  
Qian Wang ◽  
Haidong Tan ◽  
Zongbao Kent Zhao
Keyword(s):  
Isocitrate Dehydrogenase ◽  
Lipid Accumulation ◽  
Oleaginous Yeast ◽  
Phylogenetic Analyses ◽  
Dry Mass ◽  
Strain Engineering ◽  
Pcr Analysis ◽  
Lipomyces Starkeyi ◽  
Intracellular Lipids ◽  
Rapid Amplification

The oleaginous yeast Lipomyces starkeyi can accumulate intracellular lipids to over 60% of its cell dry mass under a nitrogen-limited condition. We showed that extracellular and intracellular citrate concentrations of L. starkeyi AS 2.1560 increased and the nicotinamide adenine dinucleotide – isocitrate dehydrogenase (NAD+–IDH) activity decreased at the beginning of the lipid accumulation, suggesting that the attenuation of the NAD+–IDH activity might initiate lipid storage. We next cloned the IDH gene by the methods of degenerate PCR and rapid amplification of cDNA ends. Phylogenetic analyses of the evolutionary relationships among LsIDH1, LsIDH2, and other yeast NAD+–IDHs revealed that the L. starkeyi IDH had a closer relationship with the IDHs of Yarrowia lipolytica . Further real-time PCR analysis showed that the expression levels of both LsIDH1 and LsIDH2 decreased concurrently with the evolution of cellular lipids. Our data should be valuable for understanding the biology of oleaginous yeasts and for further strain engineering of L. starkeyi.

scholarly journals Gold Bioleaching from Printed Circuit Boards of Mobile Phones by Aspergillus niger in a Culture without Agitation and with Glucose as a Carbon Source

Metals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 521 ◽  
Author(s):  
Rosalba Argumedo-Delira ◽  
Mario J. Gómez-Martínez ◽  
Brenda Joan Soto
Keyword(s):  
Aspergillus Niger ◽  
Carbon Source ◽  
Culture Medium ◽  
Printed Circuit Boards ◽  
Biological Processes ◽  
Circuit Boards ◽  
Printed Circuit ◽  
Material Resources ◽  
Low Energy Consumption ◽  
Fungal Consortium

Hydrometallurgical and pyrometallurgical processes to recover gold (Au) from cell-phone printed circuit boards (PCBs) have the disadvantage of generating corrosive residues and consuming a large amount of energy. Therefore, it is necessary to look for biological processes that have low energy consumption and are friendly to the environment. Among the biological alternatives for the recovery of Au from PCB is the use of cyanogenic bacteria and filamentous fungi in cultures with agitation. Considering that it is important to explore the response of microorganisms in cultures without agitation to reduce energy expenditure in the recovery of metals from PCB, the present investigation evaluated the capacity of Aspergillus niger MXPE6 and a fungal consortium to induce Au bioleaching from PCB in a culture medium with glucose as a carbon source and without agitation (pH 4.5). The results indicate that the treatments with PCB inoculated with the fungal consortium showed a considerable decrease in pH (2.8) in comparison with the treatments inoculated with A. niger MXPE6 (4.0). The fungal consortium showed a significantly higher Au bioleaching (56%) than A. niger MXPE6 (17%). Finally, the use of fungal consortia grown without agitation could be an alternative to recover metals from PCB, saving energy and material resources.

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