Detection of cellulase and xylanase activity in isoelectric-focused gels using agar substrate gels supported on plastic film

1984 ◽  
Vol 30 (12) ◽  
pp. 1522-1525 ◽  
Author(s):  
C. R. MacKenzie ◽  
R. E. Williams
Keyword(s):  
Congo Red ◽  
Carboxymethyl Cellulose ◽  
Xylanase Activity ◽  
Plastic Film ◽  
Sensitive Technique ◽  
Highly Sensitive ◽  
Agar Substrate

A simple, rapid, and highly sensitive technique is described for the detection of endoglucanase and endoxylanase in isoelectric-focused gels. Agar substrate gels containing 0.1% carboxymethyl cellulose or xylan are prepared on GelBond® plastic film and placed in contact with an electrofocused gel containing the enzymes under study. Hydrolysis zones in substrate gels are visualized by staining with Congo red. As little as 1 ng of enzyme is detected in 5 min using this technique.

The use of droplet digital PCR in liquid biopsies: A highly sensitive technique for MYD88 p.(L265P) detection in cerebrospinal fluid

2017 ◽  
Vol 36 (2) ◽  
pp. 429-435 ◽  
Author(s):  
Laura S. Hiemcke-Jiwa ◽  
Monique C. Minnema ◽  
Joyce H. Radersma-van Loon ◽  
N. Mehdi Jiwa ◽  
Mirthe de Boer ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Liquid Biopsies ◽  
Sensitive Technique ◽  
Highly Sensitive

scholarly journals Fibrillary Glomerulonephritis

2019 ◽  
Vol 14 (12) ◽  
pp. 1741-1750 ◽  
Author(s):  
Nicole K. Andeen ◽  
Megan L. Troxell ◽  
Maziar Riazy ◽  
Rupali S. Avasare ◽  
Jessica Lapasia ◽  
...  
Keyword(s):  
Congo Red ◽  
Primary Outcome ◽  
Multivariable Analysis ◽  
Specific Marker ◽  
Fibrillary Glomerulonephritis ◽  
Pathologic Features ◽  
Highly Sensitive ◽  
Kidney Insufficiency ◽  
Male Sex ◽  
Fibril Diameter

Background and objectivesFibrillary GN has been defined as an immune complex-mediated GN with amyloid-like fibrils larger than amyloid which are IgG positive and Congo red negative. With discovery of DNAJB9 as a highly sensitive and specific marker for fibrillary GN, the specificity of the morphologic criteria for establishing the diagnosis of fibrillary GN has come into question.Design, setting, participants, & measurementsWe sought to (1) determine anatomic characteristics that best define fibrillary GN and (2) identify clinical and pathologic features that predict outcomes.ResultsWe retrospectively reviewed kidney biopsies from patients diagnosed with fibrillary GN or suspected fibrillary GN between 1997 and 2017 (n=266, 65% female, median age 61). Approximately 11% of kidney biopsies had one or more unusual feature including monotypic deposits, Congo red positivity, or unusual fibril diameter. Fibrillary GN as a possible monoclonal gammopathy of renal significance represented <1% of cases. Immunostaining for DNAJB9 confirmed fibrillary GN in 100% of cases diagnosed as fibrillary GN and 79% of atypical cases diagnosed as possible fibrillary GN. At a median time of 24 months (interquartile range, 8–46 months) after biopsy (n=100), 53% of patients reached the combined primary outcome of ESKD or death, 18% had CKD, and 18% had partial remission. On multivariable analysis, male sex (adjusted hazard ratio [aHR], 3.82; 95% confidence interval [95% CI], 1.97 to 7.37) and eGFR were the most significant predictors of primary outcome (aHR of 8.02 if eGFR <30 ml/min per 1.73 m2 [95% CI, 1.85 to 34.75]; aHR of 6.44 if eGFR 30 to <45 ml/min per 1.73 m2 [95% CI, 1.38 to 29.99]). Immunosuppressive therapy with rituximab was significantly associated with stabilization of disease progression.ConclusionsDetection of DNAJB9 is a useful diagnostic tool for diagnosing atypical forms of fibrillary GN. The outcomes for fibrillary GN are poor and progression to ESKD is influenced predominantly by the degree of kidney insufficiency at the time of diagnosis and male sex. Rituximab may help preserve kidney function for select patients with fibrillary GN.PodcastThis article contains a podcast at https://www.asn-online.org/media/podcast/CJASN/2019_11_04_CJN03870319.mp3

FTIR-spectroscopy as a highly sensitive technique to study adsorption and desorption on ionic film and single crystal surfaces

1988 ◽  
Vol 95 (1-6) ◽  
pp. 105-108 ◽  
Author(s):  
Joachim Heidberg ◽  
Elisabeth Kampshoff ◽  
Helmut Stein ◽  
Helmut Weiss ◽  
Michael Warskulat
Keyword(s):  
Single Crystal ◽  
Ftir Spectroscopy ◽  
Crystal Surfaces ◽  
Adsorption And Desorption ◽  
Single Crystal Surfaces ◽  
Sensitive Technique ◽  
Highly Sensitive
Sign in / Sign up

Export Citation Format

Share Document