Physical factors influencing the growth of protoplasts of Entomophaga grylli

1984 ◽  
Vol 30 (11) ◽  
pp. 1315-1318 ◽  
Author(s):  
Gary B. Dunphy ◽  
June M. Chadwick
Keyword(s):  
Tissue Culture ◽  
Culture Medium ◽  
Tissue Culture Medium ◽  
Physical Parameters ◽  
Physical Factors ◽  
Insect Host ◽  
Optimum Temperature ◽  
Temperature Range ◽  
Factors Influencing ◽  
Ph Range

Selected physical parameters were examined to enhance the growth of protoplasts of Entomophaga grylli in Grace's tissue culture medium. The pH range of 6.2–6.7 permitted protoplast growth with pH 6.7 being optimal. The protoplasts grew well over a temperature range of 20–30 °C. The optimum temperature was 30 °C. Shaken cultures grew faster and in shorter chains than did stationary cultures. The optimum osmolality was 325–350 mosmol/kg. The osmolality of the serum of the insect host, Dissosteira Carolina, ranged from 318 to 389 mosmol/kg.

Cultivation of Trypanosoma brucei sspp. in semi-defined and defined media

Parasitology ◽  
1973 ◽  
Vol 67 (3) ◽  
pp. 315-331 ◽  
Author(s):  
G. A. M. Cross ◽  
J. C. Manning
Keyword(s):  
Amino Acids ◽  
Tissue Culture ◽  
Trypanosoma Brucei ◽  
Culture Medium ◽  
Defined Medium ◽  
Tissue Culture Medium ◽  
Optimum Temperature ◽  
Acid Hydrolysate ◽  
Defined Media

Semi-defined and defined media for the growth of culture forms of Trypanosoma brucei sspp. have been developed by enrichment of tissue culture medium 199 with additional vitamins, amino acids, salts and other compounds. The semi-defined medium contains an acid hydrolysate of casein: in the empirically devised defined medium the casein requirement has been circumvented by inclusion of additional vitamins and amino acids. Both media are very hypertonic. Control of pH was found to be particularly critical for growth. The optimum temperature for growth in the semidefined medium was between 25°C and 28°C, but cells would undergo one or two division cycles at 37°C.

ON TETHELIN AS A TISSUE CULTURE MEDIUM

1930 ◽  
Vol 1 (9) ◽  
pp. 289-290
Author(s):  
K. C. Richardson ◽  
E. S. Horning
Keyword(s):  
Tissue Culture ◽  
Culture Medium ◽  
Tissue Culture Medium

scholarly journals THE ANTIGENIC POTENCY OF EPIDEMIC INFLUENZA VIRUS FOLLOWING INACTIVATION BY ULTRAVIOLET RADIATION

1940 ◽  
Vol 72 (6) ◽  
pp. 729-745 ◽  
Author(s):  
Jonas E. Salk ◽  
G. I. Lavin ◽  
Thomas Francis
Keyword(s):  
Tissue Culture ◽  
Influenza Virus ◽  
Ultraviolet Radiation ◽  
Culture Medium ◽  
Tissue Culture Medium ◽  
Intraperitoneal Route ◽  
Active Virus ◽  
High Concentrations

A study of the antigenic potency of influenza virus inactivated by ultraviolet radiation has been made. Virus so inactivated is still capable of functioning as an immunizing agent when given to mice by the intraperitoneal route. In high concentrations inactivated virus appears to be nearly as effective as active virus but when quantitative comparisons of the immunity induced by different dilutions are made, it is seen that a hundredfold loss in immunizing capacity occurs during inactivation. Virus in suspensions prepared from the lungs of infected mice is inactivated more rapidly than virus in tissue culture medium. A standard for the comparison of vaccines of epidemic influenza virus is proposed.

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