Erratum: Characterization of the surface antigens of the marine fish pathogens Vibrio anguillarum and Vibrio ordalii

1984 ◽  
Vol 30 (9) ◽  
pp. 1195-1195
Author(s):  
Henrik Chart ◽  
Trevor J. Trust
Keyword(s):  
Marine Fish ◽  
Vibrio Anguillarum ◽  
Surface Antigens ◽  
Fish Pathogens ◽  
Vibrio Ordalii

Characterization of the surface antigens of the marine fish pathogens Vibrio anguillarum and Vibrio ordalii

1984 ◽  
Vol 30 (5) ◽  
pp. 703-710 ◽  
Author(s):  
Henrik Chart ◽  
Trevor J. Trust
Keyword(s):  
Outer Membrane ◽  
Chain Length ◽  
Extraction Procedure ◽  
Vibrio Anguillarum ◽  
Surface Antigens ◽  
Cross Reactivity ◽  
Antigenic Specificity ◽  
Fish Pathogens ◽  
Morphological Heterogeneity ◽  
Vibrio Ordalii

The technique of immunoblotting was used to identify the surface antigens of the marine vibrios pathogenic for fish, Vibrio anguillarum and Vibrio ordalii. Polyclonal antisera raised in rabbits to strains representing the two most common serotypes causing Vibriosis in fish in North America were used. The results demonstrated that antigenic specificity was conferred by the lipopolysaccharides, with three serotypes being displayed among the strains examined. The lipopolysaccharides of strains chosen as type species for V. anguillarum and V. ordalii displayed antigenic cross-reactivity. The morphological heterogeneity of the Vibrio lipopolysaccharides was also analyzed in silver-stained polyacrylamide gels and by intrinsic 32P-radiolabelling. Two distinct lipopolysaccharide morphologies were exhibited, one with 0 polysaccharide chains of heterogeneous chain length, the other having 0 polysaccharide chains of more uniform chain length but displaying microheterogeneity. These lipopolysaccharide morphologies corresponded to different serogroups. Two minor proteins of apparent molecular weights 49 000 – 51 000 present in outer membrane preparations isolated by the sarcosinate extraction procedure were also strong antigens, and common to all strains of V. anguillarum tested and to several strains of V. ordalii. The major outer membrane protein was a weak antigen common to both species.

Molecular characterization of Vibrio anguillarum biotype 2

1981 ◽  
Vol 27 (10) ◽  
pp. 1011-1018 ◽  
Author(s):  
Michael H. Schiewe ◽  
Jorge H. Crosa
Keyword(s):  
Vibrio Anguillarum ◽  
Virulence Plasmid ◽  
Multicopy Plasmid ◽  
Nick Translation ◽  
Fish Pathogens ◽  
Mole Percent ◽  
Discrete Nature ◽  
Sequence Relationships ◽  
Restriction Endonuclease Cleavage

Examination of polynucleotide sequence relationships among 11 strains of Vibrio anguillarum biotype 2 isolated from moribund fish in North America and Japan demonstrated the highly conserved character of this group of fish pathogens and, moreover, confirmed its discrete nature from V. anguillarum biotype 1. Additional molecular analyses of the V. anguillarum biotype 2 strains revealed the universal presence of a multicopy plasmid with a molecular mass of approximately 20 × 106 daltons and a mole percent guanine plus cytosine of 44. The plasmid of strain DF3K was representative of this molecular species and was designated pMJ101. Subsequent DNA–DNA hybridizations using nick translation-labeled pMJ101 as a probe indicated all the 20 × 106 dalton plasmids were either identical or highly conserved, and, furthermore, that pMJ101 was apparently unrelated to either the virulence plasmid, pJM1, of V. anguillarum biotype 1 or to representatives of common plasmid incompatibility groups. The lack of relatedness between pMJ101 and pJM1 was further supported by differences in their restriction endonuclease cleavage patterns.

Ultraviolet Treatment of Water for Destruction of Five Gram-Negative Bacteria Pathogenic to Fishes

1977 ◽  
Vol 34 (8) ◽  
pp. 1244-1249 ◽  
Author(s):  
G. L. Bullock ◽  
H. M. Stuckey
Keyword(s):  
Particulate Matter ◽  
Ultraviolet Irradiation ◽  
Vibrio Anguillarum ◽  
Spring Water ◽  
Aeromonas Salmonicida ◽  
Water Disinfection ◽  
Gram Negative Bacteria ◽  
Fish Pathogens ◽  
Gram Negative ◽  
Ultraviolet Treatment

Filtration (25 nm) and ultraviolet irradiation dosages of 13,100–29,400 microwatt seconds per square centimetre (μW∙s∙cm−2) effected a 99.98–100% reduction of five gram-negative fish pathogens — Aeromonas salmonicida, A. hydrophila, Vibrio anguillarum, Pseudomonas fluorescens, and the enteric redmouth organism in 12.5 °C clear spring water or spring water containing particulate matter. Filtration and a dosage of 4500 μW∙s∙cm−2 killed 99.83–100% of test strains in spring water and 4000–4750 μW∙s∙cm−2 killed 99.33–99.99% in water with particulate matter. Irradiation of unfiltered water containing particulate matter was less effective, especially at dosages of 5000 μW∙s∙cm−2 or less, which killed 97–99.94% of strains. Filtration and 13,100 μW∙s∙cm−2 irradiation of water containing A. salmonicida prevented transmission of furunculosis. Key words: ultraviolet irradiation, bacterial fish pathogens, water disinfection

scholarly journals The Fish Pathogen Vibrio ordalii Under Iron Deprivation Produces the Siderophore Piscibactin

2019 ◽  
Vol 7 (9) ◽  
pp. 313 ◽  
Author(s):  
Pamela Ruiz ◽  
Miguel Balado ◽  
Juan Carlos Fuentes-Monteverde ◽  
Alicia E. Toranzo ◽  
Jaime Rodríguez ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Iron Uptake ◽  
Peptide Mass Fingerprinting ◽  
Iron Limitation ◽  
Fish Pathogen ◽  
Fish Pathogens ◽  
Iron Deprivation ◽  
Peptide Mass ◽  
Photobacterium Damselae ◽  
Vibrio Ordalii

Vibrio ordalii is the causative agent of vibriosis, mainly in salmonid fishes, and its virulence mechanisms are still not completely understood. In previous works we demonstrated that V. ordalii possess several iron uptake mechanisms based on heme utilization and siderophore production. The aim of the present work was to confirm the production and utilization of piscibactin as a siderophore by V. ordalii. Using genetic analysis, identification by peptide mass fingerprinting (PMF) of iron-regulated membrane proteins and chemical identification by LC-HRMS, we were able to clearly demonstrate that V. ordalii produces piscibactin under iron limitation. The synthesis and transport of this siderophore is encoded by a chromosomal gene cluster homologous to another one described in V. anguillarum, which also encodes the synthesis of piscibactin. Using β-galactosidase assays we were able to show that two potential promoters regulated by iron control the transcription of this gene cluster in V. ordalii. Moreover, biosynthetic and transport proteins corresponding to piscibactin synthesis and uptake could be identified in membrane fractions of V. ordalii cells grown under iron limitation. The synthesis of piscibactin was previously reported in other fish pathogens like Photobacterium damselae subsp. piscicida and V. anguillarum, which highlights the importance of this siderophore as a key virulence factor in Vibrionaceae bacteria infecting poikilothermic animals.

scholarly journals Detection and Characterization of Autoantibodies to Neuronal Cell-Surface Antigens in the Central Nervous System

2016 ◽  
Vol 9 ◽  
Author(s):  
Marleen H. van Coevorden-Hameete ◽  
Maarten J. Titulaer ◽  
Marco W. J. Schreurs ◽  
Esther de Graaff ◽  
Peter A. E. Sillevis Smitt ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cell Surface ◽  
Neuronal Cell ◽  
Surface Antigens ◽  
Cell Surface Antigens ◽  
The Central Nervous System
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