Properties of oral asaccharolytic black-pigmented Bacteroides

1984 ◽  
Vol 30 (9) ◽  
pp. 1133-1136 ◽  
Author(s):  
D. Mayrand ◽  
G. Bourgeau ◽  
D. Grenier ◽  
J.-M. Lacroix
Keyword(s):  
Methylene Blue ◽  
Congo Red ◽  
Neutral Red ◽  
Enzymatic Activities ◽  
The Other ◽  
Other Hand ◽  
Inhibitory Agents ◽  
Group B

Bacteroides endodontalis, a newly described asaccharolytic black-pigmented Bacteroides, along with the other two recognized species of this group (B. gingivalis and B. asaccharolyticus) were studied for their susceptibility to various dyes and inhibitory agents and for some of their enzymatic activities to facilitate differentiating between them. Bacteroides endodontalis resembles B. asaccharolyticus physiologically except for the fact that the former cannot grow on media containing methylene blue, neutral red, or 3% sodium choride, whereas B. asaccharolyticus can. On the other hand, B. endodontalis and B. gingivalis can grow on a medium containing Congo red while B. asaccharolyticus cannot.

scholarly journals RESEARCH ON SHEAR PERFORMANCE OF COMPONENTS CONNECTED BY BEECH AND SELF-TAPPING SCREW COMPOSITE DOWELS

Wood Research ◽  
2021 ◽  
Vol 66 (6) ◽  
pp. 955-968
Author(s):  
XUDONG ZHU ◽  
YINGYING XUE ◽  
XUEWEN ZHANG ◽  
PENGFEI QI ◽  
JIE SHEN ◽  
...  
Keyword(s):  
Energy Consumption ◽  
Linear Equation ◽  
Failure Modes ◽  
Maximum Load ◽  
The Other ◽  
Test Results ◽  
Other Hand ◽  
Shear Performance ◽  
Group B ◽  
Better Than

This study examined the properties of components connected by beech and self-tapping screw composite dowels (group C). As a contrast, the components connected by beech dowels and self-tapping screws individually were tested. The test results indicated that the properties of the components connected by beech dowels (group B) were better than those connected by self-tapping screws (group S), except the ductility coefficient, final displacement, and energy consumption. On the other hand, the main failure modes of groups B and S were the broken beech dowel and the bent self-tapping screw, respectively. For group C, two peak values could be found which were larger than the maximum load of groups B and S, respectively. The properties of group C were better than those of groups B and S, except that the final displacement and energy consumption were located between those of groups B and S. Meanwhile, the linear equation in two unknowns have be found between groups B, S and C.

COMPOSITION OF EPIPHYSEAL CARTILAGE: III. DIFFERENCES IN ENZYMATIC ACTIVITIES OF EPIPHYSEAL AND ARTICULAR CARTILAGES OF RACHITIC CHICKS

1962 ◽  
Vol 40 (4) ◽  
pp. 419-423 ◽  
Author(s):  
J. D. Cipera ◽  
J. S. Willmer
Keyword(s):  
Alkaline Phosphatase ◽  
Articular Cartilage ◽  
Comparative Study ◽  
Adenosine Triphosphatase ◽  
Enzymatic Activities ◽  
The Other ◽  
Epiphyseal Cartilage ◽  
Other Hand ◽  
Articular Cartilages

From a comparative study of enzymatic activities in epiphyseal and articular cartilages of 3-week-old rachitic chicks it appears that the activities of pyrophosphatase and adenosine triphosphatase are approximately four times higher in epiphyseal than in articular cartilage. The greater activity of epiphyseal cartilage is even more pronounced in the cases of alkaline phosphatase and hexosamine synthetase. Phosphorylase, on the other hand, is more active in articular cartilage. The significance of these observations is discussed.

A simple technique for the preservation of vital dyes in fixed and sectioned embryos

Development ◽  
1966 ◽  
Vol 15 (2) ◽  
pp. 131-132
Author(s):  
J. Pertusa
Keyword(s):  
Methylene Blue ◽  
Ethyl Alcohol ◽  
Toluidine Blue ◽  
Trypan Blue ◽  
Simple Technique ◽  
Nile Blue ◽  
Neutral Red ◽  
The Other ◽  
Serial Sectioning ◽  
Vital Dyes

In embryological work using vital dyes it is highly desirable to be able to study the distribution of the dyes in fixed material, whether examined in toto or after embedding in paraffin and serial sectioning. However, both fixation and dehydration present problems for the preservation of colour in vitally stained cells. Some fixatives preserve some dyes but, so far as I am aware, none will preserve all the vital dyes in common use. On the other hand, ethyl alcohol destroys or dissolves all vital dyes and its use in dehydration is thus undesirable. Among the fixatives that have been proposed are those of Golowin (1902), Mitamura (1923), Parat & Painlevé (1925), and Tchéou Tai Chuin (1930) for neutral red; that of Izquierdo (1955) for toluidine blue; that of Gérard (1925) for Trypan blue; that of Turchini (1919) for methylene blue; that of Lehmann (1929) for Nile blue.

scholarly journals Association of Nasal Polyposis with Bronchial Asthma

2020 ◽  
Vol 23 (1) ◽  
pp. 11-18
Author(s):  
Md Anamul Haque ◽  
Md Kamrul Hasan Tarafder ◽  
ABM Luthful Kabir ◽  
Mohammad Harun Or Rashid ◽  
Dhiman Pramanik ◽  
...  
Keyword(s):  
Bronchial Asthma ◽  
Eosinophil Count ◽  
Nasal Polyposis ◽  
The Other ◽  
Control Group ◽  
Case Group ◽  
College Hospital ◽  
Other Hand ◽  
Group A ◽  
Group B

Objective: To find out association between Nasal polyposis and bronchial asthma. Methods: This was a cross sectional study which was carried out in the departments of Otolaryngology and Head-Neck surgery of Bangabondhu Sheikh Mujib Medical University and Dhaka Medical College Hospital during the period of February 2014 to July 2014. A Total 230 patients were selected according to selection criteria among which 30 subjects had nasal polyposis (designated as group A or cases) and 200 subjects had no nasal polyposis (designated as group B or control group). Results: In group (A), asthma was found in 30.0% cases. On the other hand, asthma was found in 2.5% of control group (B). The association of nasal polyposis with bronchial asthma was highly significant (p<.05). In both group 40.0% patients were within the 38-47 year’s age group. In case group, 80.0% patients had any sorts of allergic history on the other hand, only 15.0% patients had allergic history in control group. High eosinophil count was found 66.7% patients suffering from nasal polyposis with bronchial asthma and in comparison to patients suffering from nasal polyposis without bronchial asthma had high eosinophil count 42.85%. In group A High IgE level was found 66.7% patients suffering from nasal polyposis with bronchial asthma, on the other hand, 42.85% had IgE level among patients suffering from nasal polyposis without bronchial asthma. Conclusion: Patients with nasal polyposis and asthma are significantly associated. Both serum IgE and total circulating eosinophil count are significant associated with nasal polyposis and bronchial asthma. Bangladesh J Otorhinolaryngol; April 2017; 23(1): 11-18

ANDROGEN METABOLISM IN THE EPITHELIAL AND STROMAL COMPONENTS OF THE HUMAN HYPERPLASTIC PROSTATE

1981 ◽  
Vol 91 (1) ◽  
pp. 23-32 ◽  
Author(s):  
F. K. HABIB ◽  
ANN L. TESDALE ◽  
G. D. CHISHOLM ◽  
A. BUSUTTIL
Keyword(s):  
Prostate Gland ◽  
Hydroxysteroid Dehydrogenase ◽  
Enzymatic Activities ◽  
The Other ◽  
Asymmetric Distribution ◽  
Androgen Metabolism ◽  
Main Site ◽  
Other Hand

The reduced and oxidized metabolites of testosterone and dihydrotestosterone were measured in the stromal and epithelial components of 23 human hyperplastic prostates. Our studies indicate differences in the hormonal metabolic patterns of the stroma and epithelium of the resected specimens when compared with tissues obtained retropubically. Testosterone 5α-reductase was evenly distributed between the two components of the specimens obtained retropubically whereas the 3α(β)-hydroxysteroid dehydrogenase was predominantly located in the stroma. The measurements on the resected specimens suggest, on the other hand, that the bulk of the 5α-reductase and 3α(β)-hydroxysteroid dehydrogenase activities were confined to the stroma although these activities were considerably lower than those measured in the corresponding components of the retropubically obtained specimens. The conversion of testosterone to androstenedione was negligible in all the samples analysed. We therefore conclude that the stroma is the main site for the transformation of dihydrotestosterone to the androstanediol epimers and that the asymmetric distribution of the 3α(β)-hydroxysteroid dehydrogenase may be instrumental in the development of hyperplasia in the prostate gland. Furthermore, the results of this study indicate that electro-resection impairs the enzymatic activities of the tissue.

scholarly journals Papillary Variation in Coliform Bacteria

1941 ◽  
Vol 41 (5-6) ◽  
pp. 497-508 ◽  
Author(s):  
F. H. Stewart
Keyword(s):  
Neutral Red ◽  
The Other ◽  
Coliform Bacteria ◽  
Short Exposure ◽  
Logarithmic Phase ◽  
Liquid Media ◽  
Similar Variation ◽  
Short Summary ◽  
Other Hand

Papillaryvariation includes all heritable changes of character which take place in the papillae of bacterial colonies and the corresponding variations in liquid media.The following is a short summary of what is known about these variations. Papillary variation was first observed inB. coli-mutabile, and other coliform bacteria show the same variations. This race forms white colonies on lactose neutral-red plates. After 1–5 days’ incubation minute papillae, which become red and increase in size, appear on their surfaces. Subcultures from thesepapillae give colonies of two kinds, white and red. The white colonies continue to throw red, the reds breed true (Neisser, 1906; Massim, 1907). Similar variation to different sugars occurs in other related bacteria:B. dysentenaeSonne varies to lactose,B. dysentenaeMexner to maltose,B. paracolonto saccharose,B. coh-mutabile, B. acidi-lacticiandB. typhosusto dulcite (Twort, 1907; Reiner Miiller, 1909; Burn, 1910; Stewart, 1926, 1927). Vacation takes place only on the sugar concerned, but, once it has taken place, the character remains fixed, even after long growth on other media. Variation is not determined by length of exposure to the sugar. It occurs at (or after) the end of the logarithmic phase. If this phase is kept going by daily subculture (in the appropriate sugar) the bacteria of the chain carried on from culture to culture do not vary, however long they are exposed (Stewart, 1927; Deere, Dulaney & Michelson, 1939). On the other hand, if the logarithmic phase is curtailed by heavy inoculation of a culture, variation occurs early (Stewart, 1927). If the bacterium is grown on a plate without sugar until the colonies are well formed and the sugar is then added, with care not to disturb the colonies, variants appear after very short exposure (Stewart, 1927, 1928). A variation 33–2 may be completed in, two distinct steps; thus a race ofB. typhosusformed white colonies on dulcite with pink papillae, subcultures from which gave white and pink colonies; the pink colonies formed dark red papillae from which on subculture came pink and dark red colonies, the latter bred true.

STUDY OF METHYLENE BLUE INTERACTION WITH SYNTHETIC POLYNUCLEOTIDE POLY(rA)-POLY(rU)

2020 ◽  
Vol 54 (2 (252)) ◽  
pp. 112-117
Author(s):  
M.A. Parsadanyan ◽  
M.A. Shahinyan ◽  
A.P. Antonyan
Keyword(s):  
Methylene Blue ◽  
Fluorescence Spectroscopy ◽  
The Other ◽  
Binding Modes ◽  
Other Hand ◽  
Non Linear ◽  
Order Of Magnitude ◽  
Synthetic Polynucleotide ◽  
Specific Ligand

A study of the interaction of methylene blue (MB) with poly(rA)-poly(rU) by the method of fluorescence spectroscopy has been carried out. The data obtained revealed that MB, being a DNA-specific ligand, can bind to double-stranded regions of RNA. In this regard, as in the case of DNA, semi-intercalation was the most preferable mechanism for the binding of this ligand to poly(rA)-poly(rU). On the other hand, non-linear curves of dependence of F0/F on concentration of polynucleotide might result from two binding modes, the second of which was probably of an electrostatic nature. Proceeding from the data obtained, the value of KSV was revealed to be almost an order of magnitude less than for DNA, which may indicate that RNA is a less preferable target for MB.

scholarly journals Construction and Functional Activities of Chimeric Mouse-Human Immunoglobulin G and Immunoglobulin M Antibodies against the Neisseria meningitidis PorA P1.7 and P1.16 Epitopes

2003 ◽  
Vol 71 (10) ◽  
pp. 5714-5723 ◽  
Author(s):  
Terje E. Michaelsen ◽  
Øistein Ihle ◽  
Karen Johanne Beckstrøm ◽  
Tove K. Herstad ◽  
Jan Kolberg ◽  
...  
Keyword(s):  
Immunoglobulin M ◽  
The Other ◽  
Human Immunoglobulin ◽  
Chimeric Mouse ◽  
Protective Effects ◽  
Functional Activities ◽  
Other Hand ◽  
V Genes ◽  
Group B

ABSTRACT We studied the in vitro protective activities of human immunoglobulin G1 (IgG1), IgG3, and IgM antibodies against group B meningococci by constructing sets of chimeric mouse-human antibodies (chIgG1, chIgG3, and chIgM, respectively) with identical binding regions against the P1.7 and P1.16 epitopes on PorA. This was done by cloning the V genes of three mouse hybridoma antibodies and subsequently transfecting vectors containing the homologous heavy- and light-chain genes into NSO cells. Cell clones secreting intact human chIgG1, chIgG3, or chIgM antibodies originating from three parent mouse antibodies were isolated. The functional affinities appeared to be similar for all human isotypes and surprisingly also for the pentameric chIgM antibody. chIgG1 exhibited greater serum bactericidal activity (SBA) than chIgG3, while chIgG3 was more efficient in inducing a respiratory burst (RB) associated with opsonophagocytosis than chIgG1 was. On the other hand, chIgM exhibited SBA similar to that of chIgG1, but it exhibited much higher RB activity than chIgG3 and chIgG1 exhibited. The antibodies against the P1.16 epitope were more efficient in terms of SBA than the antibodies against the P1.7 epitope were; thus, 10- to 40-fold-lower concentrations of antibodies against P1.16 than of antibodies against P1.7 were needed to induce SBA. On the other hand, antibodies against these epitopes were equally effective in inducing RB. Our results revealed differences in the functional activities of human chIgG1, chIgG3, and chIgM antibodies against meningococci, which might influence their protective effects against meningococcal disease.

COMPOSITION OF EPIPHYSEAL CARTILAGE: III. DIFFERENCES IN ENZYMATIC ACTIVITIES OF EPIPHYSEAL AND ARTICULAR CARTILAGES OF RACHITIC CHICKS

1962 ◽  
Vol 40 (1) ◽  
pp. 419-423 ◽  
Author(s):  
J. D. Cipera ◽  
J. S. Willmer
Keyword(s):  
Alkaline Phosphatase ◽  
Articular Cartilage ◽  
Comparative Study ◽  
Adenosine Triphosphatase ◽  
Enzymatic Activities ◽  
The Other ◽  
Epiphyseal Cartilage ◽  
Other Hand ◽  
Articular Cartilages

From a comparative study of enzymatic activities in epiphyseal and articular cartilages of 3-week-old rachitic chicks it appears that the activities of pyrophosphatase and adenosine triphosphatase are approximately four times higher in epiphyseal than in articular cartilage. The greater activity of epiphyseal cartilage is even more pronounced in the cases of alkaline phosphatase and hexosamine synthetase. Phosphorylase, on the other hand, is more active in articular cartilage. The significance of these observations is discussed.

scholarly journals Microcolony Formation by Single-Cell Synechococcus Strains as a Fast Response to UV Radiation

2011 ◽  
Vol 77 (21) ◽  
pp. 7533-7540 ◽  
Author(s):  
Cristiana Callieri ◽  
Andrea Lami ◽  
Roberto Bertoni
Keyword(s):  
Single Cell ◽  
Uv Radiation ◽  
Fast Response ◽  
The Other ◽  
Low Light ◽  
Content Type ◽  
Other Hand ◽  
Light Intensities ◽  
Group I ◽  
Group B

ABSTRACTUV radiation (UVR) has different effects on prokaryotic cells, such as, for instance, filamentation and aggregation in bacteria. Here we studied the effect of UVR on microcolony formation in two freshwaterSynechococcusstrains of different ribotypes (group B and group I) and phycobiliprotein compositions (phycoerythrin [PE] and phycocyanin [PC]). Each strain was photoacclimated at two light intensities, low light (LL) (10 μmol m−2s−1) and moderate light (ML) (100 μmol m−2s−1). The cultures were exposed for 6 days to treatments with UVR or without UVR. PE-richSynechococcusacclimated to LL had a low carotenoid/chlorophylla(car/chl) ratio but responded faster to UVR treatment, producing the highest percentages of microcolonies and of cells in microcolonies. Conversely, the same strain acclimated to ML, with a higher car/chl ratio, did not aggregate significantly. These results suggest that microcolony formation by PE-richSynechococcusis induced by UVR if carotenoid levels are low. PC-richSynechococcusformed a very low percentage of microcolonies in both acclimations even with low car/chl ratio. The different responses of the twoSynechococcusstrains to UVR depend on their pigment compositions. On the other hand, this study does not exclude that UVR-induced microcolony formation could also be related to specific ribotypes.

Sign in / Sign up

Export Citation Format

Share Document