Siderophore production by Proteus mirabilis

1984 ◽  
Vol 30 (8) ◽  
pp. 1046-1051 ◽  
Author(s):  
Lisa P. Evanylo ◽  
Solomon Kadis ◽  
James R. Maudsley
Keyword(s):  
Absorption Spectrum ◽  
Proteus Mirabilis ◽  
Biosynthetic Pathway ◽  
Agar Medium ◽  
Ultraviolet Absorption Spectrum ◽  
Gas Chromatograph ◽  
Isolation And Characterization ◽  
Solid Agar ◽  
Layer Chromatography

Studies on the isolation and characterization of Proteus mirabilis siderophores provided no evidence that these bacteria synthesize catechol- or hydroxamate-type siderophores. However, gas chromatograph analysis in conjunction with mass spectroscopy revealed the presence of α-hydroxyisovaleric acid, a previously unknown metabolite. Additional substantiating evidence for the presence of α-hydroxyisovaleric acid in these bacteria was obtained from experiments involving the use of thin-layer chromatography and an ultraviolet absorption spectrum. This compound was found to be capable of removing iron from the synthetic chelator, ethylene-diamine-di-orthohydroxyphenyl acetic acid, and supplying that iron to the bacteria both in a solid agar medium and in a liquid medium. Proteus mirabilis was found to possess an enzyme capable of catalyzing the reaction by which α-hydroxyisovaleric acid is converted to α-ketoisovaleric acid, an intermediate in the valine biosynthetic pathway.

Métabolisme intracellulaire et extracellulaire de l'acide 3-indolylacétique dans les cultures aseptiques ou non aseptiques de pointes de racine du Pisum sativum

1968 ◽  
Vol 46 (8) ◽  
pp. 969-978 ◽  
Author(s):  
G. F. Collet
Keyword(s):  
Absorption Spectrum ◽  
Pisum Sativum ◽  
Indoleacetic Acid ◽  
Specific Activity ◽  
High Specific Activity ◽  
Ultraviolet Absorption Spectrum ◽  
Root Tips ◽  
Pea Seedlings ◽  
Oxidase Inhibition ◽  
Layer Chromatography

Studies on indoleacetic acid (IAA) metabolism with root tips of 3-day-old pea seedlings were carried out by thin-layer chromatography and 14C-labelled auxin of high specific activity at 10−6 M. After 16-h incubation, five metabolites of IAA could be recognized in the tissues and two in solution; one metabolite was identified as o-formamidoacetophenone from its chromatographic Rf and ultraviolet absorption spectrum. Under sterile conditions the rate of breakdown of IAA is higher in the medium than under nonsterile conditions (even after removal of the tissues). We noted, too, a decrease of the action of IAA on the growth. The decreased activity of solution under nonsterile conditions is believed to be due to the production of oxidase inhibition by microorganisms.

scholarly journals Isolation and Characterization of Indigenous Lactic Acid Bacteria from Sago Wastewater

2018 ◽  
Author(s):  
Pauline Destinugrainy Kasi ◽  
Ariandi ◽  
Heni Mutmainnah
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Agar Medium ◽  
Gram Positive ◽  
Processing Industry ◽  
Isolation And Purification ◽  
South Sulawesi ◽  
Isolation And Characterization

Sago extraction processing into sago flour is generally still done in a traditional or semi-mechanical way, with less attention to aspects of hygiene, and resulted sago fermentation spontaneously. This study aimed to isolate and characterize indigenous lactic acid bacteria (LAB) from sago wastewater which obtained directly from the traditional sago processing industry in Malangke Barat, South Sulawesi. The sago wastewater was stored for 1, 3 and 7 days of fermentation. The research was conducted on the LAB isolation and purification on MRS agar medium supplemented with 0.5% CaCO3. Two purified putative LAB isolate were each obtained from 1 day fermentation and 3 days fermentation. The LAB isolate from 1 day fermentation has milky white colors and smooth-round colonies, while LAB isolates from 3 days fermentation has creamy colors and smooth-round colonies. Both of the isolate were gram positive-rods, non-motile and negative catalase reaction.

A Yellow Discoloration of Cooked Cured Meat Products - Isolation and Characterization of the Causative Organism

1989 ◽  
Vol 52 (6) ◽  
pp. 392-395 ◽  
Author(s):  
ALLAN M. WHITELEY ◽  
MARIETTE D. D'SOUZA
Keyword(s):  
Meat Products ◽  
Heat Processing ◽  
Causative Organism ◽  
Refrigerated Storage ◽  
Anaerobic Conditions ◽  
Isolation And Characterization ◽  
Cured Meat ◽  
Layer Chromatography ◽  
Aerobic And Anaerobic

An organism has been isolated in pure culture that is capable of causing a yellow discoloration on a cooked cured meat substrate under aerobic and anaerobic conditions. This organism has subsequently been identified as a member of the genus Streptococcus. The characteristics were such that a tentative identification as Streptococcus faecium, sub-species casseliflavus was made. The organism is extremely heat resistant and could survive a heat processing of 71.1°C for 20 min. The yellow discoloration on a vacuum packaged luncheon style meat takes 3 to 4 wk to become apparent under refrigerated storage. The pigment was extracted and separated by thin layer chromatography (TLC). The evidence points to the compound being carotenoid in nature.

scholarly journals The Mycobacterium tuberculosis purine biosynthetic pathway: isolation and characterization of the purC and purL genes

Microbiology ◽  
1996 ◽  
Vol 142 (9) ◽  
pp. 2439-2447 ◽  
Author(s):  
M. Jackson ◽  
F.-X. Berthet ◽  
I. Otal ◽  
J. Rauzier ◽  
C. Martin ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Biosynthetic Pathway ◽  
Isolation And Characterization

Preparation, isolation, and characterization of permethylated galactosides and fucosides

1999 ◽  
Vol 77 (3) ◽  
pp. 319-325 ◽  
Author(s):  
Daniel D Asres ◽  
Hélène Perreault
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Characterization Methods ◽  
Isolation And Characterization ◽  
Nmr Data ◽  
Layer Chromatography ◽  
Structural Aspects

Sugar analysis involving permethylation followed by methanolysis can lead to significant results, given that permethylated monosaccharide standards are available for comparison of spectral and chromatographic data. Such standards are not readily commercially available, especially the furanoside forms. This note describes the isolation and characterization of permethylated pyranoside and furanoside species of D(+)-galactose and L(-)-fucose. Separation of the isomers was optimized using a combination of column chromatography and continuous elution thin-layer chromatography (TLC). TLC, gas chromatography - mass spectrometry, and 1H nuclear magnetic resonance (NMR) spectroscopy were used as the characterization methods. The isolation of furanosides is emphasized, since no specific NMR data have been reported on those to date, while several reports have already discussed the structural aspects of pyranosides.Key words: permethylation, monosaccharide, GC-MS, TLC, NMR.

Isolation and Characterization of the Photoalteration Products of cis- and trans-Chlordane

1975 ◽  
Vol 58 (1) ◽  
pp. 6-9
Author(s):  
Francis I Onuska ◽  
Michael E Comba
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Thin Layer ◽  
High Yield ◽  
Isolation And Characterization ◽  
The Individual ◽  
Cis And Trans ◽  
Layer Chromatography

Abstract Ultraviolet irradiation of cis- and trares-chlordane yielded 3 photolysis products. The expected half-caged analog of cis-chlordane was formed in high yield, and 2 minor photoproducts of trans-chlordane were observed. One of these products was a half-caged isomer. The individual photoproducts were isolated by thin layer chromatography and characterized by infrared, nuclear magnetic resonance, and mass spectrometry.

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