Construction of a shuttle vector and expression in Streptomyces lividans of the sulfonamide-resistance gene derived from Escherichia coli plasmid pSAS1206

1984 ◽  
Vol 30 (4) ◽  
pp. 515-518 ◽  
Author(s):  
F. Shareck ◽  
A. Sasarman ◽  
Claude Vézina
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Shuttle Vector ◽  
Streptomyces Lividans ◽  
Hybrid Plasmid ◽  
Sulfonamide Resistance ◽  
Coli Plasmid ◽  
Sulfonamide Resistance Gene

We have constructed a hybrid plasmid using Streptomyces lividans plasmid pIJ 101 and Escherichia coli plasmid pSAS1206. This plasmid, designated pFSH102, is able to replicate in both hosts and the sulfonamide-resistance gene encoded by pSAS1206 is phenotypically expressed in S. lividans.

scholarly journals Incidence of the Recently Described Sulfonamide Resistance Gene sul3 among German Salmonella enterica Strains Isolated from Livestock and Food

2004 ◽  
Vol 48 (7) ◽  
pp. 2712-2715 ◽  
Author(s):  
Beatriz Guerra ◽  
Ernst Junker ◽  
Reiner Helmuth
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Resistance Gene ◽  
Salmonella Enterica ◽  
Bacterial Populations ◽  
First Report ◽  
Strong Potential ◽  
Sulfonamide Resistance ◽  
Sulfonamide Resistance Gene

ABSTRACT The sul3 gene recently described in Escherichia coli was found in 22 of 512 (4.3%) German Salmonella isolates from different regions and sources and of different serotypes, antimicrobial resistance phenotypes, and genomic groups. This is the first report on the prevalence of sul3 among Salmonella strains, and the findings support the strong potential of this determinant to spread within bacterial populations.

A novel shuttle vector for Streptomyces spp. and Escherichia coli as a tool in site-directed mutagenesis

1992 ◽  
Vol 38 (4) ◽  
pp. 350-353 ◽  
Author(s):  
A. Moreau ◽  
F. W. Paradis ◽  
R. Morosoli ◽  
F. Shareck ◽  
D. Kluepfel
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Shuttle Vector ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Gene Encoding ◽  
Single Stranded Dna ◽  
E Coli ◽  
Streptomyces Spp ◽  
Ampicillin Resistance Gene

This paper describes the construction and utilization of a novel shuttle vector for Streptomyces spp. and Escherichia coli as a useful vector in site-directed mutagenesis. The shuttle vector pIAFS20 (6.7 kb) has the following features: a replicon for Streptomyces spp., isolated from plasmid pIJ702; the thiostrepton-resistance gene as a selective marker in Streptomyces; the ColE1 origin, allowing replication in E. coli; and the ampicillin-resistance gene as a selective markerin E. coli. Vector pIAFS20 also contains the phage fl intergenic region, which permits production of single-stranded DNA in E. coli after superinfection with helper phage M13K07. Moreover, the lac promoter is located in front of the multiple cloning sites cassette, allowing eventual expression of the cloned genes in E. coli. After mutagenesis and screeningof the mutants in E. coli, the plasmids can be readily used to transform Streptomyces spp. As a demonstration, a 3.2-kb DNA fragment containing the gene encoding the xylanase A from Streptomyces lividans 1326 was inserted into pIAFS20, and the promoter region of this gene served as a target for site-directed mutagenesis. The two deletions reported here confirm the efficiency of this new vector as a tool in mutagenesis. Key words: shuttle vector, single-stranded DNA, site-directed mutagenesis, Streptomyces spp., Escherichia coli.

scholarly journals Detection and Characterization of Clinical Bordetella trematum Isolates from Chronic Wounds

Pathogens ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 966
Author(s):  
Christian Buechler ◽  
Claudio Neidhöfer ◽  
Thorsten Hornung ◽  
Marcel Neuenhoff ◽  
Marijo Parčina
Keyword(s):  
Resistance Gene ◽  
Clinical Significance ◽  
Chronic Wounds ◽  
Beta Lactamase ◽  
Broth Microdilution ◽  
Bacterial Genomes ◽  
Sulfonamide Resistance ◽  
Increased Susceptibility ◽  
Sulfonamide Resistance Gene

Bordetella trematum is a relatively newly discovered and potentially frequently overlooked Bordetella species, mostly isolated from chronic wounds and predominantly in those of the lower extremities. Its susceptibility profile and clinical significance is still debated, given the limited amount of available data. We contribute providing a molecular and phenotypical analysis of three unique clinical B. trematum isolates detected between August 2019 and January 2020 to aid the matter. Cryo-conserved isolates were subcultured and re-identified using various routine means of identification. Bacterial genomes were fully Illumina-sequenced and phenotypical susceptibility was determined by broth microdilution and gradient-strip tests. All isolates displayed increased susceptibility to piperacillin–tazobactam (<2/4 mg/L), imipenem (<1 mg/L), and meropenem (<0.047 mg/L), whereas they displayed decreased susceptibility to all tested cephalosporins and fluoroquinolones (according to PK-PD, EUCAST 10.0 2020). One isolate carried a beta-lactamase (EC 3.5.2.6) and a sulfonamide resistance gene (sul2) and cells displayed resistance to ampicillin, ampicillin/sulbactam, and trimethoprim/sulfamethoxazole. All isolates carried genes conferring decreased susceptibility to aminoglycosides (aadA), fosfomycin (fosA) and fluoroquinolones (gyrB EC 5.99.1.3). Awareness that B. trematum can be resistant to trimethoprim/sulfamethoxazole is warranted.

Expression of the sulfonamide resistance gene from plasmid R46

Plasmid ◽  
1990 ◽  
Vol 23 (1) ◽  
pp. 35-41 ◽  
Author(s):  
François Guerineau ◽  
Louise Brooks ◽  
Philip Mullineaux
Keyword(s):  
Resistance Gene ◽  
Sulfonamide Resistance ◽  
Sulfonamide Resistance Gene
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