The relationship between yeast cell size and cell division in Candida albicans

1984 ◽  
Vol 30 (2) ◽  
pp. 192-203 ◽  
Author(s):  
W. LaJean Chaffin
Keyword(s):  
Growth Rate ◽  
Candida Albicans ◽  
Cell Division ◽  
Doubling Time ◽  
Time Lapse ◽  
Nonlinear Approach ◽  
Wide Range ◽  
Mean Size ◽  
The Relationship ◽  
Doubling Times

The mean size and percentage of budded and unbudded cells of Candida albicans grown in batch culture over a wide range of doubling times have been measured. Cell volume decreased with increased doubling time and a nonlinear approach to an asymptotic minimum was observed. When cells were separated by age according to bud scars, each age showed a similar decrease. During each cell division cycle, size increased slowly during both budded and unbudded periods so that each generation was significantly larger than the preceeding. There was no difference in size between the parent portion of budded cells and unbudded cells of the same age. Time-lapse photomicroscopy of cells growing on solid medium showed that cells divide asymmetrically with larger parents having a shorter subsequent cycle time than the smaller daughter, although the time utilized for bud formation was similar. When cells were shifted from a medium supporting a low growth rate and small size to a medium supporting a faster growth rate and larger size, both budded and unbudded cells increased significantly in size. As the doubling time increased, both the budded and unbudded portions of parental and daughter cycles increased.

Reversion of the MR variant of Candida albicans

1969 ◽  
Vol 15 (9) ◽  
pp. 1051-1054
Author(s):  
Rosalee Ireland ◽  
A. Sarachek
Keyword(s):  
Growth Rate ◽  
Candida Albicans ◽  
Cell Division ◽  
Incubation Temperature ◽  
High Rate ◽  
Type Condition ◽  
Wild Type ◽  
Spontaneous Repair

Previous studies have indicated that the minute-rough (MR) colonial variant of Candida albicans arises through mutation of an extrachromosomal hereditary determinant. The MR variant's high rate of reversion to the wild-type condition suggests either that (a) the determinant undergoes frequent spontaneous repair or that (b) variant cells bear both normal and mutant determinants which segregate at cell division. Analyses presented here of the effects of growth rate, nutrition, incubation temperature, and metabolic antagonists upon reversion favor the view that reversion is due to repair of mutant determinants.

The Relationship between Growth Speed and Ambient Humidity in Convective Self-assembly

2010 ◽  
Vol 1273 ◽  
Author(s):  
Hans D. Robinson ◽  
Kai Chen ◽  
Stefan V. Stoianov
Keyword(s):  
Growth Rate ◽  
Self Assembly ◽  
Standard Technique ◽  
Crystal Growth Rate ◽  
Growth Speed ◽  
Linear Dependency ◽  
Ambient Relative Humidity ◽  
Wide Range ◽  
Assembly Technique ◽  
The Relationship

AbstractWe present a variation of a standard convective self-assembly technique, where the drying meniscus is restricted by a straight-edge located approximately 100 μm above the substrate adjacent to the drying zone. We find this technique to yield films at roughly twice the growth rate compared to the standard technique. We attribute this to differing local evaporation rates in the two cases. We also investigate how the crystal growth rate depends on ambient relative humidity and find a clear linear dependency, which we attribute to the length of the drying zone being constant over a wide range of humidities.

Pattern of end growth of the fission yeast Schizosaccharomyces pombe

1990 ◽  
Vol 36 (6) ◽  
pp. 390-394 ◽  
Author(s):  
Hisao Miyata ◽  
Machiko Miyata ◽  
Byron F. Johnson
Keyword(s):  
Cell Cycle ◽  
Growth Rate ◽  
Schizosaccharomyces Pombe ◽  
Key Words ◽  
Fission Yeast ◽  
Doubling Time ◽  
Time Lapse ◽  
Critical Value ◽  
Wild Type ◽  
Time Lapse Photomicrography

The patterns of end growth of individual cells of Schizosaccharomyces pombe, wild-type cells (strain 972 h−), cells exposed to 8 mM hydroxyurea, and cdc mutants (cdc11-123 and cdc2-33), were investigated by time-lapse photomicrography. It was reconfirmed that there are three patterns of end growth: cells growing at the old end, at the new end, and at both ends from the beginning of the cell cycle. Cells that initiated growth at the old (new) end increased their growth rate at the new (old) end and became constant in their growth rate at the old (new) end when cells had their growth rate higher than a critical value: 0.08, 0.09, 0.08, and 0.11 μm/min in wild-type cells, cells exposed to hydroxyurea, cdc11-123 cells, and cdc2-33 cells, respectively. The critical value is proportional to the doubling time in length. Key words: extension, growth, fission yeast.

scholarly journals A general quantitative relation linking bacterial cell growth and the cell cycle

2019 ◽  
Author(s):  
Hai Zheng ◽  
Yang Bai ◽  
Meiling Jiang ◽  
Taku A. Tokuyasu ◽  
Xiongliang Huang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Growth Rate ◽  
Cell Division ◽  
Bacterial Cell ◽  
Cell Mass ◽  
Quantitative Relation ◽  
Growth Physiology ◽  
Dividing Cells ◽  
Bacterial Cell Cycle ◽  
Doubling Times

The foundation of bacterial cell cycle studies has long resided in two interconnected dogmas between biomass growth, DNA replication, and cell division during exponential growth: the SMK growth law that relates cell mass (a measure of cell size) to growth rate1, and Donachie’s hypothesis of a growth-rate-independent initiation mass2. These dogmas have spurred many efforts to understand their molecular bases and physiological consequences3–12. Most of these studies focused on fast-growing cells, with doubling times shorter than 60 min. Here, we systematically studied the cell cycle of E. coli for a broad range of doubling times (24 min to over 10 hr), with particular attention on steady-state growth. Surprisingly, we observed that neither dogma held across the range of growth rates examined. In their stead, a new linear relation unifying the slow- and fast-growth regimes was revealed between the cell mass and the number of cell divisions it takes to replicate and segregate a newly initiated pair of replication origins. This and other findings in this study suggest a single-cell division model, which not only reproduces the bulk relations observed but also recapitulates the adder phenomenon established recently for stochastically dividing cells13–15. These results allowed us to develop quantitative insight into the bacterial cell cycle, providing a firm new foundation for the study of bacterial growth physiology.

Growth Rate of Soft Tissue Sarcomas: A Quantitative Study of 44 Local Recurrences

1980 ◽  
Vol 66 (2) ◽  
pp. 215-222 ◽  
Author(s):  
Emanuele Galante ◽  
Angelo Milani ◽  
Angelo Attili
Keyword(s):  
Growth Rate ◽  
Soft Tissue ◽  
Quantitative Study ◽  
Doubling Time ◽  
Geometric Mean ◽  
Soft Tissue Sarcomas ◽  
Local Recurrences ◽  
Significant Difference ◽  
Previously Treated ◽  
Doubling Times

Local recurrences of soft tissue sarcomas in 44 patients were studied to quantify their growth rate. The geometric mean of the doubling time of 32 cases previously treated with surgery only (14.8 days) was similar to that of 10 cases treated with surgery plus radiotherapy (14.1 days), and was less than that of most malignant human tumors. There was no significant difference in doubling times of first and successive recurrences. From the study it appears that the site of the primary and the age of the patient may affect the behavior of the tumor.

scholarly journals Studies on the Growth of Tissues In Vitro

1939 ◽  
Vol 16 (2) ◽  
pp. 232-249
Author(s):  
C. M. POMERAT ◽  
E. N. WILLMER
Keyword(s):  
Growth Rate ◽  
Cell Division ◽  
Growth Inhibition ◽  
Carbohydrate Metabolism ◽  
Sodium Azide ◽  
Methyl Glyoxal ◽  
Sodium Pyruvate ◽  
Sodium Malonate ◽  
The Relationship

1. Growth of chick periosteal fibroblasts is inhibited by 0.002 M solutions of glyceraldehyde, benzaldehyde, butyl aldehyde, propyl aldehyde and methyl glyoxal. 2. The growth is not inhibited by sodium pyruvate, lactate, propionate, glycerate or benzoate. 3. Sodium pyruvate does not affect the growth under the conditions of the experiments, nor does it alter the inhibition brought about by glyceraldehyde. 4. Growth is inhibited by sodium fluoride and sodium iodoacetate in the concentrations in which these inhibit glucolysis. Growth inhibition does not correspond to inhibition of phosphorylation. 5. Growth is not inhibited by 0.008 M phlorizin. 6. Growth is not immediately inhibited by an atmosphere containing 95% CO and 5% O2. 7. Growth is not immediately inhibited by 0.002 M sodium azide, 0.002 M HCN or by 0.01 M sodium malonate. Azide and cyanide reduce the growth rate after some hours. 8. Growth is not accelerated by the addition of 0.006 M sodium fumarate. 9. Growth is inhibited by 0.01 M sodium ferricyanide. 10. The relationship between carbohydrate metabolism and growth by cell division is discussed in the light of these results.

scholarly journals Relationship between COVID-19 death toll doubling time and national BCG vaccination policy

2020 ◽  
Author(s):  
Yutaka Akiyama ◽  
Takashi Ishida
Keyword(s):  
Doubling Time ◽  
Statistical Test ◽  
Bacillus Calmette Guerin ◽  
Vaccination Policy ◽  
Detection Rates ◽  
Bcg Vaccination ◽  
Death Toll ◽  
Significant Difference ◽  
The Relationship ◽  
Doubling Times

AbstractIn this manuscript, we showed a statistically significant difference of the doubling times of the death toll between the group of countries with national universal Bacillus Calmette-Guerin (BCG) vaccination and the group without it for recent years. Based on a statistical test, the distributions of the doubling time of these two groups were significantly different (p=0.007). Miller et al. reported the relationship between BCG vaccination and mortality for COVID-19 based on deaths per million inhabitants. However, they did not take into account the differences in COVID-19 detection rates among the countries and the epidemic stages of the countries. Therefore we used a doubling time of the death toll as a more stable indicator instead. We also investigated the dependency of the BCG strains. Among the 42 BCG-vaccinated countries, the median doubling time of the eight countries using “Tokyo 172-1” strain at least partially (Japan, Iraq, Malaysia, South Korea, Philippines, Saudi Arabia, Pakistan, and Bangladesh) was 7.2 days, and that of the other 34 vaccinated countries was 5.5 days. Their distributions were also significantly different (p=0.026).

The Problem of Identifying and Modeling the Relationship between Monetary Factor and Inflation in the Russian Economy

2008 ◽  
pp. 61-76
Author(s):  
A. Porshakov ◽  
A. Ponomarenko
Keyword(s):  
Money Supply ◽  
Money Demand ◽  
Russian Economy ◽  
Long Run ◽  
Complex Approach ◽  
Wide Range ◽  
Long Time ◽  
The Relationship ◽  
Supply Side Factors

The role of monetary factor in generating inflationary processes in Russia has stimulated various debates in social and scientific circles for a relatively long time. The authors show that identification of the specificity of relationship between money and inflation requires a complex approach based on statistical modeling and involving a wide range of indicators relevant for the price changes in the economy. As a result a model of inflation for Russia implying the decomposition of inflation dynamics into demand-side and supply-side factors is suggested. The main conclusion drawn is that during the recent years the volume of inflationary pressures in the Russian economy has been determined by the deviation of money supply from money demand, rather than by money supply alone. At the same time, monetary factor has a long-run spread over time impact on inflation.

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