Isolation and genetic analysis of Azospirillum brasilense Nif− mutants

1983 ◽  
Vol 29 (8) ◽  
pp. 968-972 ◽  
Author(s):  
P. Jara ◽  
B. Quiviger ◽  
P. Laurent ◽  
C. Elmerich
Keyword(s):  
Genetic Analysis ◽  
Host Range ◽  
Azospirillum Brasilense ◽  
Nitrogenase Activity ◽  
Plasmid Vector ◽  
Ecori Fragment ◽  
Broad Host Range ◽  
Broad Host Range Plasmid ◽  
Ethylmethane Sulfonate ◽  
Derivatives Of

After ethylmethane sulfonate mutagenesis of Azospirillum brasilense strain 7000, mutants devoid of nitrogenase activity were isolated. Partial diploids were constructed by introducing plasmids pAB35 and pAB36 into the Nif− mutants. The two plasmids were derivatives of the broad host-range plasmid vector pRK290. Plasmid pAB35 contained a 6.7 kilobase pairs (kb) EcoRI fragment which carried the nifHDK gene cluster cloned from strain 7000. Plasmid pAB36 contained the same fragment from which a 2.6-kb PstI fragment that likely covers nifK, and a part of nifD was deleted. The restoration of a Nif+ phenotype by pAB35, but not by pAB36, was observed in the case of mutant 7571, which might be impaired in a structural gene for the nitrogenase complex.

scholarly journals A broad host range plasmid vector that does not encode replication proteins

2002 ◽  
Vol 211 (1) ◽  
pp. 91-95 ◽  
Author(s):  
Eza Kalyaeva ◽  
Irina Bass ◽  
Gennady Kholodii ◽  
Vadim Nikiforov
Keyword(s):  
Host Range ◽  
Plasmid Vector ◽  
Broad Host Range ◽  
Replication Proteins ◽  
Broad Host Range Plasmid

Multiple mechanisms for expression of incompatibility by broad-host-range plasmid RK2

1982 ◽  
Vol 152 (3) ◽  
pp. 1078-1090
Author(s):  
R Meyer ◽  
M Hinds
Keyword(s):  
Dna Replication ◽  
Host Range ◽  
Plasmid Dna ◽  
Plasmid Vector ◽  
Broad Host Range ◽  
Plasmid Maintenance ◽  
Broad Host Range Plasmid ◽  
Expression Of Genes ◽  
Plasmid Partitioning ◽  
Plasmid Rk2

By cloning fragments of plasmid DNA, we have shown that RK2 expresses incompatibility by more than one mechanism. One previously identified (R. J. Meyer, Mol. Gen, Genet. 177:155--161, 1979; Thomas et al., Mol. Gen. Genet. 181:1--7, 1981) determinant for incompatibility is linked to the origin of plasmid DNA replication. When cloned into a plasmid vector, this determinant prevents the stable inheritance of a coresident RK2. However, susceptibility to this mechanism of incompatibility requires an active RK2 replicon and is abolished if another replicator is provided. We have also cloned a second incompatibility determinant, encoded within the 54.1- to 56.4-kilobase region of RK2 DNA, which we call IncP-1(II). An RK2 derivative remains sensitive to IncP-1(II), even when it is not replicating by means of the RK2 replicon. The 54.1- to 56.4-kilobase DNA does not confer susceptibility to the IncP-1(II) mechanism, nor does it encode a detectable system for efficient plasmid partitioning. The incompatibility may be related to the expression of genes mapping in the 54.1- to 56.4-kilobase region, which are required for plasmid maintenance and suppression of plasmid-encoded killing functions.

scholarly journals Structural, molecular, and genetic analysis of the kilA operon of broad-host-range plasmid RK2.

1991 ◽  
Vol 173 (11) ◽  
pp. 3463-3477 ◽  
Author(s):  
P Goncharoff ◽  
S Saadi ◽  
C H Chang ◽  
L H Saltman ◽  
D H Figurski
Keyword(s):  
Genetic Analysis ◽  
Host Range ◽  
Broad Host Range ◽  
Broad Host Range Plasmid ◽  
Plasmid Rk2

Host-specific effects of the korA-korB operon and oriT region on the maintenance of miniplasmid derivatives of broad host-range plasmid RK2

Plasmid ◽  
1989 ◽  
Vol 21 (2) ◽  
pp. 99-112 ◽  
Author(s):  
Thomas J. Schmidhauser ◽  
David H. Bechhofer ◽  
David H. Figurski ◽  
Donald R. Helinski
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Specific Effects ◽  
Broad Host Range Plasmid ◽  
Derivatives Of ◽  
Plasmid Rk2

Replication of derivatives of the broad host range plasmid RK2 in two distantly related bacteria

Plasmid ◽  
1983 ◽  
Vol 9 (3) ◽  
pp. 325-330 ◽  
Author(s):  
Thomas J. Schmidhauser ◽  
Marcin Filutowicz ◽  
Donald R. Helinski
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Broad Host Range Plasmid ◽  
Derivatives Of ◽  
Plasmid Rk2

Broad-host-range plasmid vector for the in vitro construction of transcriptional/translational lac fusions

Gene ◽  
1985 ◽  
Vol 34 (2-3) ◽  
pp. 219-226 ◽  
Author(s):  
Francis E. Nano ◽  
W.D. Shepherd ◽  
M.M. Watkins ◽  
S.A. Kuhl ◽  
S. Kaplan
Keyword(s):  
Host Range ◽  
Plasmid Vector ◽  
Broad Host Range ◽  
Broad Host Range Plasmid

Overcoming the metabolic load associated with the presence of plasmid DNA in the plant growth promoting rhizobacteriumPseudomonas putidaGR12-2

1995 ◽  
Vol 41 (7) ◽  
pp. 624-628 ◽  
Author(s):  
Yuwen Hong ◽  
J. J. Pasternak ◽  
Bernard R. Glick
Keyword(s):  
Plant Growth ◽  
Plasmid Vector ◽  
Plant Growth Promoting Rhizobacteria ◽  
Broad Host Range ◽  
Metabolic Load ◽  
Broad Host Range Plasmid ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Lactamase Gene ◽  
Derivatives Of

When the broad host range plasmid vector pGSS15 was used to genetically transform the plant growth promoting rhizobacterium Pseudomonas putida GR12-2, the transformants were physiologically debilitated. It was postulated that the expression of the β-lactamase gene of pGSS15 caused a metabolic load resulting in the impaired functioning of the bacterium. To test this hypothesis, derivatives of pGSS15 that either lack the β-lactamase gene (pYH122) or in which a β-glucosidase gene was substituted for the β-lactamase gene (pYH124) were constructed and examined to see whether their presence also impaired the functioning of P. putida GR12-2. On the basis of growth rates, siderophore production, and the ability to stimulate canola root elongation in sterile growth pouches, neither of the newly constructed plasmids debilitated P. putida GR12-2. In addition, P. putida GR12-2 transformed with pYH124 facilitated the proliferation of the bacterium in minimal medium containing cellobiose at low temperature. This latter trait may enable P. putida GR12-2 to persist in the soil in competition with other microorganisms.Key words: plant growth promoting rhizobacteria, PGPR, bacterial fertilizer, soil bacteria, metabolic load, β-glucosidase

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