Competitive ability, effectiveness, and succinate – triphenyl tetrazolium chloride reductase activity of mutant strains of chick-pea (Cicer arietinum L.) Rhizobium sp.

1983 ◽  
Vol 29 (7) ◽  
pp. 735-745 ◽  
Author(s):  
Raman Rai ◽  
V. Prasad ◽  
S. D. Sharma ◽  
I. C. Shukla
Keyword(s):  
Competitive Ability ◽  
Nitrogenase Activity ◽  
Reductase Activity ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
Chick Pea ◽  
Cicer Arietinum L ◽  
Reactive Iron ◽  
Mutant Strains ◽  
Rhizobium Sp

Chick-pea Rhizobium strain RG 4 was treated with 200 μg/mL of N-methyl-N-nitro-N′-nitrosoguanidine and 14 mutant strains isolated. Two mutant strains were found to be nonnodulating in the absence of certain amino acids. On the basis of nodulation, two mutant strains (M 38 and M 46) were found to be equally competitive against each other. Mutant strains M 38 and M 46 when applied together produced the maximum nodulation, nitrogenase activity, phenanthroline-reactive iron, and least tetrazolium reductase activity as compared to other pairwise combinations of mutant strains. It was also observed that the correlation between tetrazolium reductase and nitrogenase activity was highly negative.

Competitiveness and effectiveness of carbendazim-resistant mutant and indigenous Rhizobiumstrains of chick pea (Cicer arietinutnLinn.) in calcareous soil

1985 ◽  
Vol 104 (2) ◽  
pp. 341-344 ◽  
Author(s):  
R. Rai
Keyword(s):  
Calcareous Soil ◽  
Nitrogenase Activity ◽  
Reductase Activity ◽  
Dry Weight ◽  
Resistant Mutant ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
Chick Pea ◽  
Active Iron ◽  
Mutant Strains

SummaryChick pea Rhizobiumstrain RG 4 was treated with 30 /tg/ml of N-nitrosoguanidine and four mutants isolated were resistant to 400 /tg carbendazim/ml. Only two of the mutants (M 1 and M 4) retained the capacity to form nodules. On the basis of number of nodules, their dry weight, nitrogenase activity, triphenyl tetrazolium chloride reductase activity and active iron content of nodules, mutant strains M 1 and M 4 were found to be equally effective for nitrogen fixation both with and without carbendazim (bavistine) and resulted in significantly greater grain yield than the corresponding uninoculated treatments.

Transformation of Azotobacter vinelandii strains unable to fix nitrogen with Rhizobium spp. DNA

1978 ◽  
Vol 24 (3) ◽  
pp. 209-214 ◽  
Author(s):  
William J. Page
Keyword(s):  
Transcriptional Control ◽  
Deoxyribonucleic Acid ◽  
Azotobacter Vinelandii ◽  
Nitrogenase Activity ◽  
Nitrogen Fixing ◽  
Wild Type ◽  
Spontaneous Reversion ◽  
Mutant Strains ◽  
Rhizobium Spp ◽  
Rhizobium Sp

The phenotypes of Azotobacter vinelandii ATCC 12837 strains defective in nitrogen fixation (Nif−) were characterized by intrageneric transformation with known Nif− strains of A. vinelandii OP. These former mutant strains were used as recipients for intergeneric transformation by deoxyribonucleic acid (DNA) prepared from Rhizobium spp. to determine if the rhizobia would transform the Azotobacter Nif− phenotypes to Nif+. The frequency of Nif+ transformants using Rhizobium DNA was always less than the frequency using Azotobacter wild-type DNA but was greater than the spontaneous reversion frequency. The Azotobacter Nif+ recombinants also were stable. DNA from all of the Rhizobium spp. transformed to Nif+Azotobacter mutants defective in the nitrogenase component I (molybdoferredoxin); however, some recombinants had a lower nitrogenase activity and a delayed nitrogenase depression time. Mutants defective in the pleiotrophic transcriptional control of both nitrogenase components were transformed to Nif+ by the asymbiotic nitrogen fixing Rhizobium sp. 32H1 and 41A1, but not the symbiotic nitrogen-fixing species. The significance of these results and the possible future applications of this system are discussed.

scholarly journals Potential Fertilization Capacity of Two Grapevine Varieties: Effects on Agricultural Production in Designation of Origin Areas in the Northwestern Iberian Peninsula

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 961
Author(s):  
J. Antonio Cortiñas Rodríguez ◽  
María Fernández-González ◽  
Estefanía González-Fernández ◽  
Rosa A. Vázquez-Ruiz ◽  
F. Javier Rodríguez-Rajo ◽  
...  
Keyword(s):  
Airborne Pollen ◽  
Germination Rate ◽  
Pollen Viability ◽  
Weather Conditions ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
The Mean ◽  
Study Sites ◽  
Fertilization Capacity

In the present study, we analyzed the main parameters related with the potential fertilization ability of two grapevine varieties, Godello and Mencía, during the years 2017 and 2018. The research was carried out in two vineyards of the Galician winegrowing Designation of Origin areas of Ribeiro and Ribeira Sacra. Ten vines of each variety were selected for bunch and flower counting, pollen calculations, pollen viability studies by means of aceto-carmine (AC) stain and 2, 3, 5-triphenyl tetrazolium chloride (TTC) methods, and the determination of their germination rate. In all vineyards the 50% fruitset was reached, except for Godello in Cenlle during 2017. The mean coulure value was higher for Godello (40.5%) than for Mencía (31%). Analyzing the pollen production per plant and airborne pollen levels, we observed important discordances between them, which can be due to the influence of weather conditions and be related with self-pollination processes. We found important differences on pollen viability depending on the applied method and variety, with higher values for the AC method than the TTC for both varieties in all study plots, and higher values for Mencía variety than Godello. Regarding germination rates, we observed a marked reduction in 2017 with respect to 2018, in all study sites and for both varieties. The analyzed parameters were useful to explain the different productive abilities of Godello and Mencía varieties in the two studied bioclimatic regions of Ribeiro and Ribeira Sacra.

A Histochemical Study of Dehydrogenase Activity in the Pectoralis Major Muscle of the Pigeon and certain other Vertebrate Skeletal Muscles

1958 ◽  
Vol s3-99 (48) ◽  
pp. 469-473
Author(s):  
J. C. GEORGE ◽  
K. S. SCARIA
Keyword(s):  
Dehydrogenase Activity ◽  
Skeletal Muscles ◽  
Pectoralis Major Muscle ◽  
Breast Muscle ◽  
Muscle Fibres ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
Unique System ◽  
Oxidative Processes ◽  
The Individual

Certain dehydrogenases in the breast muscle of the pigeon and fowl and the leg muscle of the fowl and frog were studied histochemically by the use of 2:3:5: triphenyl tetrazolium chloride. The dehydrogenase activity was found to have a relationship with the colour and the mitochondrial content of the individual muscle fibres. In the pigeon breast muscle, however, the broad white fibres did not show the presence of any of the enzymes studied. It is therefore concluded that these fibres in the pigeon breast muscle are a unique system in which none of the oxidative processes concerned takes place; they cannot be considered as analogous to the white fibres of the other muscles studied.

Oxygen uptake and tetrazolium reduction during skin cycle of the mouse

1965 ◽  
Vol 209 (1) ◽  
pp. 231-235 ◽  
Author(s):  
Ira M. Dushoff ◽  
John Payne ◽  
Falls B. Hershey ◽  
Robert C. Donaldson
Keyword(s):  
Oxygen Consumption ◽  
Mouse Skin ◽  
Growth Cycle ◽  
Triphenyl Tetrazolium Chloride ◽  
Intact Skin ◽  
Tetrazolium Chloride ◽  
Quantitative Measurements ◽  
Close Relationship ◽  
Coefficient Of Regression ◽  
Tetrazolium Reduction

Quantitative measurements of oxygen consumption and triphenyl tetrazolium chloride reduction by intact skin were made during various stages of the skin cycle of the mouse. Values are highest during early anagen, when growth is most rapid: Qo2 is 2.47 ± 0.10 (mean ± se) and TTC is 537 ± 24.6. In late anagen the activities decrease 40–50%: Qo2 is 1.24 ± 0.10 and TTC is 315 ± 20.8. There is a close relationship between tetrazolium reduction and oxygen consumption during all phases of the growth cycle of mouse skin. The coefficient of regression of TTC on Qo2 is 163.2 ( P < .001).

Effect of Dietary Raw Chick Pea (Cicer arietinum L.) Seeds on Broiler Performance and Blood Constituents

2012 ◽  
Vol 11 (4) ◽  
pp. 294-297 ◽  
Author(s):  
Tamador. A. Algam ◽  
Khadiga. A. Abdel A ◽  
B.M. Dousa ◽  
S.M. Elawad ◽  
A.M. Fadel Elseed
Keyword(s):  
Cicer Arietinum ◽  
Broiler Performance ◽  
Chick Pea ◽  
Blood Constituents ◽  
Cicer Arietinum L
Sign in / Sign up

Export Citation Format

Share Document