scholarly journals Cell-mediated immune response in gonococcal infection

1981 ◽  
Vol 27 (1) ◽  
pp. 76-80 ◽  
Author(s):  
Patricia J. Landolfo ◽  
Thomas J. Marrie ◽  
Norma A. Nelson ◽  
Allan R. Ronald
Keyword(s):  
Control Group ◽  
Cell Mediated Immunity ◽  
Migration Inhibition ◽  
Effector Cells ◽  
Peripheral Leukocyte ◽  
Cell Mediated Immune Response ◽  
Maximum Inhibition ◽  
And Migration ◽  
Leukocyte Migration Inhibition Test ◽  
Migration Of Cells

A peripheral leukocyte migration inhibition test has been used to demonstrate cellular immunity to a protein component of Neisseria gonorrhoeae. Human leukocytes served as effector cells in an agarose method to distinguish antigen-sensitive from nonsensitive individuals. Leukocytes, preincubated with antigen, were placed in wells in medium 199 agarose. After 18 h of incubation the migration index was calculated by dividing the area of migration of cells preincubated with antigen by the area of migration of the control cells.Significant migration inhibition was demonstrated for 16 of the 30 patients with uncomplicated gonorrhoea. Maximum inhibition was obtained 7–10 days following the onset of symptoms and the duration of that response varied from 14 to more than 40 days. There was a statistically significant positive correlation between the number of previous infections and migration inhibition. A control group, which included individuals with Neisseria meningitidis infection, showed an insignificant response to this gonococcal antigen.Although these results indicate the presence of cell-mediated immunity, the significance of this response in the protection of the host or in the pathogenesis of gonococcal disease has yet to be determined.

MicroRNA‐33b replacement effect on growth and migration inhibition in ovarian cancer cells

2021 ◽  
Author(s):  
Jin Liu ◽  
Weiming Wang ◽  
Limin Chen ◽  
Yachai Li ◽  
Shuimiao Zhao ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Migration Inhibition ◽  
And Migration ◽  
Effect On Growth

scholarly journals Bu Shen Yang Xue Prescription Has Treating Effect on Endometrial Cancer through FSH/PI3K/AKT/Gankyrin/HIF-α/cyclinD 1 Pathway in Ishikawa Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-10
Author(s):  
Yue-qun Chen ◽  
Hua-li Fei ◽  
Hong-li Zhu
Keyword(s):  
Endometrial Cancer ◽  
Endometrial Carcinoma ◽  
Cancer Cell ◽  
Nude Mice ◽  
Follicle Stimulating Hormone ◽  
Control Group ◽  
Model Group ◽  
Migration Ability ◽  
Ishikawa Cells ◽  
And Migration

Background. The formulation of Bu Shen Yang Xue (BSYX) has been clinically used in treating gynecologic disease in China, especially for the development of the endometrium. Endometrial carcinoma is the most common malignant tumor of the female genital tract in developed countries. And few studies have been reported on the antitumor activity of BSYX. Therefore, this study aimed to investigate the effect of BSYX on endometrial cancer and make an initial discussion of the underlining mechanisms in Ishikawa cells. Methods and Results. Firstly, 60 SPF female nude mice were randomly divided into control group, model group, BSYX group, and positive group. The models of subcutaneous tumor xenograft of nude mice were established by injection of human endometrial carcinoma cell line Ishikawa tumor cell suspension. Compared with model group, BSYX reduced effectively tumor volume and changed pathological feature in mice tumor issue. Meanwhile, proteins from tumor issues were detected by western blot analysis. The protein levels of follicle-stimulating hormone receptor (FSHR), p-Akt/Akt, Gankyrin, and cyclinD1 in the model group were higher than those in control group but the expression in BSYX group was lower than that in the model group. The hypoxia inducible factor alpha (HIF-α) protein level in the model group was lower than those in control group and upregulated in BSYX group. In addition, Ishikawa cells were cultured and then exposed to follicle-stimulating hormone (FSH), LY294002, a highly selective PI3K inhibitor and serum containing BSYX, respectively. LY294002 and BSYX markedly decreased the cancer cell viability and migration ability and increased the apoptosis rate. FSH promoted the cancer cell ability and migration ability. LY294002 and BSYX evidently downregulated the proteins levels of FSHR, p-Akt/Akt, Gankyrin, and cyclinD1 and upregulated the expression of HIF-α protein, and FSH was on the opposite. Conclusions. Taken together, our results showed that the formulation of BSYX had antitumor effect on endometrial cancer in vivo and in vitro and was related with FSH/PI3K/AKT/Gankyrin/HIF-α/cyclinD1 transduction pathway.

scholarly journals Proliferation of human malignant hematopoietic cells in immunodeficient mice: suppression by antibody to pluripotent K-562 leukemia cells involves direct cytolysis and effector cells

Blood ◽  
1983 ◽  
Vol 61 (6) ◽  
pp. 1045-1053 ◽  
Author(s):  
BB Lozzio ◽  
EA Machado ◽  
J Mitchell ◽  
CB Lozzio ◽  
CJ Wust ◽  
...  
Keyword(s):  
Gamma Globulin ◽  
Hematopoietic Cells ◽  
Leukemia Cells ◽  
Control Group ◽  
Fab Fragment ◽  
Lymphoma Cells ◽  
Effector Cells ◽  
Immunodeficient Mice ◽  
Immune Gamma Globulin

Abstract Six human hematopoetic cell lines were successfully heterotransplanted into athymic (nude) and asplenic-athymic (lasat) neonatal mice. The tumors arising from leukemia and lymphoma cells could then be serially transplanted into adult nude mice. Seven days after the fourth serial mouse passage, each mouse was treated with goat immune gamma globulin against K-562 cells. One control group was treated similarly, but with nonimmune (normal) gamma globulin, while another control group was not treated. The goat gamma globulin was not toxic for nude and lasat mice, and the immune, but not the normal, gamma globulin suppressed local subcutaneous growth of myelosarcomas, lymphosarcomas, and Burkitt lymphoma cells. On the other hand, the growth of lung, breast, and prostatic carcinomas and a melanoma of human origin were not altered by the immune gamma globulin. Since suppression of cell growth occurred equally well in decomplemented mice, a complement-mediated cytotoxicity apparently cannot be considered as responsible for the abrogation. The Fab fragment of the immunoglobulin did not suppress the growth of the myelosarcomas. We conclude that antibody suppression of the in vivo proliferation was specific for malignant hematopoietic cells and that the Fc portion of IgG is necessary for in vivo cytolysis of leukemia cells. The most probable mechanisms are direct antibody cytolysis and antibody-dependent macrophage-mediated cytotoxicity.

scholarly journals Expression of AIM2 in rheumatoid arthritis and its role on fibroblast-like synoviocyte

2020 ◽  
Author(s):  
fujuan qiu ◽  
Chen Yong ◽  
Qiu Fujuan ◽  
Zhao Xiaofeng ◽  
Xiao Changhong
Keyword(s):  
Rheumatoid Arthritis ◽  
Mtt Assay ◽  
Control Group ◽  
Expression Levels ◽  
Potential Target ◽  
Caspase 1 ◽  
Significant Difference ◽  
And Migration ◽  
Fibroblast Like Synoviocytes ◽  
Proliferation And Migration

Abstract Background To determine whether any differences of AIM2 inflammasome expression levels between rheumatoid arthritis (RA) and osteoarthritis (OA) and investigate the effects of AIM2 when transferred into RA fibroblast-like synoviocytes (RA-FLS).Methods Serum AIM2 levels between OA and RA patients were compared by ELISA. Different expression levels of AIM2, ASC, Caspase-1 and IL-1β between RA and OA synovium were semi-quantified by RT-qPCR and immunohistochemical (IHC) staining. IHC staining were recorded by H scores, and determine the correlation with ESR and CRP levels of RA patients. SiRNA AIM2 was transferred to RA-FLS and observe its effects on proliferation and migration by MTT assay and transwell test respectively.Results In RA sera, no significant difference was observed between OA and RA patients. However, in affected knee synovium, AIM2, ASC, Caspase-1 and IL-1β were expressed higher in RA than that of OA. Plus, H score of AIM2, ASC, and IL-1β were positively correlated to ESR and CRP levels in RA patients. After transferred AIM2 siRNA to FLS and incubation for 48 hours, the proliferation of FLS were significantly inhibited, and the apoptosis rate were significantly increased compared to FLS in control group. However, no effect on migration was detected.Conclusions AIM2 participated in the proliferation of FLS, and might be a potential target for therapy.

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