Production of indole-3-propanoic acid and 3-(p-hydroxyphenyl)propanoic acid by Clostridium sporogenes: a convenient thin-layer chromatography detection system

1980 ◽  
Vol 26 (4) ◽  
pp. 448-453 ◽  
Author(s):  
Joanne J. Jellet ◽  
Thomas P. Forrest ◽  
Ian. A. Macdonald ◽  
Thomas J. Marrie ◽  
Lillian V. Holdeman
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Detection System ◽  
Spot Test ◽  
The Other ◽  
Propanoic Acid ◽  
Clostridium Sporogenes ◽  
Clostridial Species ◽  
Layer Chromatography

Indole-3-propanoic acid (IPA), 3-(p-hydroxyphenyl)propanoic acid (HPPA), and 3-phenylpropanoic acid (PPA) were present in the spent bacterial media of Clostridium sporogenes (20/20 strains) and C. cylindrosporum (1/1 strains), but absent in 32 other clostridial species (74 strains) tested. Both IPA and HPPA (but not PPA) could be readily detected by thin-layer chromatography and p-hydroxybenzaldehyde spray reagent. IPA forms a scarlet complex with p-hydroxybenzaldehyde which shifts to purple and remains stable for up to 6 weeks. IPA can be detected in acidified extracts of C. sporogenes by a simple spot test. The structures of IPA and HPPA were confirmed by nuclear magnetic resonance (nmr) and mass spectroscopy and their formation was detected by the absorbance at 280 nm. Addition of one of the precursor amino acids (L-tryptophan, L-tyrosine, or L-phenylalanine) to the medium greatly enhanced formation of the corresponding deaminated acid and depressed the formation of the other two acids. The products IPA, HPPA, and PPA, at 10−3M, and spent bacterial media were negative in the direct Ames's assay for mutagenicity and noncytotoxic towards MRC-S cells.

scholarly journals A validated quantification of triclosan in toothpaste using high-performance thin-layer chromatography and a 48-bit fatbed scanner

2021 ◽  
Author(s):  
Barbara Anders ◽  
Sabrina Doll ◽  
Bernd Spangenberg
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Detection System ◽  
Flatbed Scanner ◽  
Butyl Ether ◽  
Quantification Method ◽  
Direct Measurements ◽  
Layer Chromatography

AbstractWe present a densitometric quantification method for triclosan in toothpaste, separated by high-performance thin-layer chromatography (HPTLC) and using a 48-bit flatbed scanner as the detection system. The sample was band-wise applied to HPTLC plates (10 × 20 cm), with fluorescent dye, Merck, Germany (1.05554). The plates were developed in a vertical developing chamber with 20 min of chamber saturation over 70 mm, using n-heptane–methyl tert-butyl ether–acetic acid (92:8:0.1, V/V) as solvent. The RF value of triclosan is hRF = 22.4, and quantification is based on direct measurements using an inexpensive 48-bit flatbed scanner for color measurements (in red, green, and blue) after plate staining with 2,6-dichloroquinone-4-chloroimide (Gibbs' reagent). Evaluation of the red channel makes the measurements of triclosan very specific. For linearization, an extended Kubelka–Munk expression was used for data transformation. The range of linearity covers more than two orders of magnitude and is between 91 and 1000 ng. The separation method is inexpensive, fast and reliable.

THIN-LAYER CHROMATOGRAPHY OF AMINO ACIDS: A REVIEW

2012 ◽  
Vol 35 (11) ◽  
pp. 1497-1516 ◽  
Author(s):  
S. A. Bhawani ◽  
M. N. Mohamad Ibrahim ◽  
O. Sulaiman ◽  
R. Hashim ◽  
A. Mohammad ◽  
...  
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography
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