Physical mapping of the genes controlling adenovirus paracrystal formation

1978 ◽  
Vol 24 (11) ◽  
pp. 1381-1386 ◽  
Author(s):  
Joseph Weber
Keyword(s):  
Virus Strain ◽  
Physical Mapping ◽  
Temperature Sensitive ◽  
Determining Factor ◽  
Strain Dependent

The genetic properties and physical mapping of the genes controlling adenovirus-induced paracrystals were examined by means of a new, simplified technique. The induction of paracrystals was virus-strain dependent and blocked by several temperature-sensitive mutations. Using interserotypic recombinants between a paracrystal-producing and a non-producing strain of adenovirus, the principal paracrystal determining factor was mapped between coordinates 30 and 44 on the 100-unit adenovirus genome.

Genetic analysis of temperature-sensitive mutants of HSV-1: The combined use of complementation and physical mapping for cistron assignment

Virology ◽  
1983 ◽  
Vol 130 (2) ◽  
pp. 290-305 ◽  
Author(s):  
Sandra K. Weller ◽  
Doris P. Aschman ◽  
Wendy R. Sacks ◽  
Donald M. Coen ◽  
Priscilla A. Schaffer
Keyword(s):  
Genetic Analysis ◽  
Physical Mapping ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants ◽  
Combined Use ◽  
Hsv 1

scholarly journals Inoculation of hamsters with a temperature sensitive (ts) mutant of parainfluenza 3 virus

1972 ◽  
Vol 70 (2) ◽  
pp. 229-234 ◽  
Author(s):  
N. Zygraich ◽  
Michèle Lobmann ◽  
C. Huygelen
Keyword(s):  
Nasal Mucosa ◽  
Virus Strain ◽  
Temperature Sensitive ◽  
Potential Candidate ◽  
Serological Response ◽  
Virulent Virus ◽  
Ts Mutant ◽  
Intranasal Vaccine

SUMMARYThe multiplication pattern of a temperature-sensitive (ts) mutant of bovine parainfluenza 3 virus was studied in hamsters and compared with that of a virulent virus strain. The ts mutant was recovered regularly from the nasal mucosa and not from the lungs, whereas the virulent virus multiplied in the lungs as well as in the nasal mucosa.The serological response induced by the mutant was comparable to that obtained after inoculation of the virulent virus.This ts mutant may be a potential candidate for a live intranasal vaccine against bovine parainfluenza 3 infection.

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