Comparative virulence and immunodiffusion analysis of Petriellidium boydii (Shear) Malloch strains isolated from feedlot manure and a human mycetoma

1978 ◽  
Vol 24 (7) ◽  
pp. 856-863 ◽  
Author(s):  
R. G. Bell
Keyword(s):  
Gastric Mucin ◽  
Soluble Antigen ◽  
Internal Organs ◽  
Swiss Mice ◽  
Immunodiffusion Analysis ◽  
Common Antigens

The virulence to mice of a Petriellidium boydii strain isolated from feedlot manure was compared with that of a strain from a human mycetoma. Washed spores suspended in 5% hog gastric mucin were injected intraperitoneally into non-inbred female albino Swiss mice. In 21 days, the mycetoma strain killed 95% of the test animals whereas injection of eight times as many feedlot strain spores resulted in only 28% mortality. A progressive, exclusively sinistral (left-sided) torticollis was the most obvious symptom of experimental petriellidiosis. Mycelia bearing annellospores were observed in PAS-stained sections of most internal organs, including the brains, of the test animals. Immunodiffusion analyses were conducted on soluble antigen preparations with concentrated antisera produced in rabbits using live spore suspensions. The two strains-contained common antigens but the proportions of two were significantly different.

scholarly journals MODIFICATION OF THE HOMOTYPIC SPECIFICITY OF POLIOMYELITIS COMPLEMENT-FIXING ANTIGENS BY HEAT

1956 ◽  
Vol 104 (1) ◽  
pp. 99-120 ◽  
Author(s):  
Nathalie J. Schmidt ◽  
Edwin H. Lennette ◽  
Keyword(s):  
Hela Cell ◽  
Cell Cultures ◽  
Soluble Antigen ◽  
Native State ◽  
Poliomyelitis Virus ◽  
Specific Antigens ◽  
Common Antigens ◽  
High Degree

Fluid obtained from HeLa cell cultures infected with poliomyelitis viruses served as a complement-fixing antigen. When used in the native state, i.e. untreated in any way, the fluids acted as homotypically specific antigens. When heated, however, the antigenicity was broadened and a high degree of heterotypic reactivity was encountered. Data are presented indicating that the observed group reactivity was apparently based on common antigens shared by the three immunologic types of poliomyelitis virus. This reactivity appeared to be specific for the poliomyelitis viruses. No evidence was obtained in preliminary experiments that heating of the antigens releases a "soluble" antigen responsible for the group reactivity.

scholarly journals Canines vaccinated against visceral leishmaniasis develop a serological response to the Leishmania braziliensis antigen

2018 ◽  
Vol 39 (2) ◽  
pp. 573
Author(s):  
Julia De Assis Pinheiro ◽  
Silas Garcia Giori ◽  
Sayanne Luns Hatum de Almeida ◽  
Rafael Assis de Souza ◽  
Ana Paula Madureira ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Cross Reactivity ◽  
Leishmania Braziliensis ◽  
Soluble Antigen ◽  
Serological Response ◽  
Serum Samples ◽  
Asymptomatic Group ◽  
Post Vaccination ◽  
Common Antigens ◽  
Positive Frequency

American cutaneous leishmaniasis (ACL) is a zoonosis caused by Leishmania, a protozoan. Common antigens occur in the strains found in America, which allow antigenic cross-reactivity. Therefore, multivalent vaccines can be used for this pathogen. In this study, we investigated the efficacy of two different commercial vaccines for visceral leishmaniasis to induce an immune response to the soluble L. (Viannia) braziliensis antigens. In 2014, 70 seronegative dogs from the municipality of Iúna (Espírito Santo State, Brazil) were vaccinated and serologically evaluated by ELISA and immunoblotting by using the soluble antigen of L. braziliensis. Of the 121 dogs initially selected, only 70 received vaccination because 51 dogs tested positive by ELISA, yielding a positive frequency of 42.14% in the asymptomatic group. These 70 dogs were divided into two equal groups and administered three doses of each vaccine, according to the manufacturers’ instructions. We found that the sera of dogs immunized with three doses of both vaccines A and B had antibodies against the soluble antigens of L. (V.) braziliensis, as determined by ELISA and immunoblotting 120 days post vaccination. Antibodies produced in response to vaccines A and B were found in 22/35 and 18/35 serum samples, respectively, at T1 (120 days), while 7/35 and 4/35 serum samples tested positive at T2 (240 days). Furthermore, immunoblotting allowed us to differentiate between vaccinated and asymptomatic dogs.

Alteration of Mitochondria in Oxythiamine-Induced, Acute Thiamine Deficiency in the Mouse

1976 ◽  
Vol 34 ◽  
pp. 284-285
Author(s):  
Itaru Watanabe ◽  
Dante G. Scarpelli
Keyword(s):  
Electron Microscopy ◽  
Adult Male ◽  
Thiamine Deficiency ◽  
Light And Electron Microscopy ◽  
Fatty Change ◽  
Deficient Diet ◽  
Subcutaneous Injections ◽  
Swiss Mice ◽  
Time Period ◽  
Ad Libitum

Acute thiamine deficiency was produced in mice by the administration of oxythiamine, a thiamine analogue, superimposed upon a thiamine deficient diet. Adult male Swiss mice (30 gm. B.W.) were fed with a thiamine deficient diet ad libitumand were injected with oxythiamine (170 mg/Kg B.W.) subcutaneously on days 4 and 10. On day 11, severe lassitude and anorexia developed, followed by death within 48 hours. The animals treated daily with subcutaneous injections of thiamine (300 μg/Kg B.W.) from day 11 through 15 were kept alive. Similarly, feeding with a diet containing thiamine (600 μg/Kg B.W./day) from day 9 through 17 reversed the condition. During this time period, no fatal illness occurred in the controls which were pair-fed with a thiamine deficient diet.The oxythiamine-treated mice showed a significant enlargement of the liver, which weighed approximately 1.5 times as much as that of the pair-fed controls. By light and electron microscopy, the hepatocytes were markedly swollen due to severe fatty change and swelling of the mitochondria.

Numerical and experimental investigation on internal membrane pressure wave inside sealed structure

2013 ◽  
Vol 61 (3) ◽  
pp. 613-621 ◽  
Author(s):  
W. Barnat
Keyword(s):  
Experimental Investigation ◽  
Pressure Wave ◽  
Pressure Increase ◽  
Internal Organs ◽  
Pressure Impulse ◽  
Internal Membrane ◽  
Contaminated Area

Abstract The article presents an approach to modeling the internal membrane pressure wave inside a sealed structure. During an explosion near a vehicle when a pressure wave reaches a hull, a pressure wave inside arises due to the hull’s bottom and the deformation of sides. They act like the piston - membrane. This membrane transfers the pressure impulse into the vehicle’s interior. A pressure increase causes the damage of internal organs or even death of occupants. In case of an armor penetration the pressure increase may be even larger. One of basic methods to protect a crew is to open hatches. However, such a method cannot be used in a contaminated area.

Rheumatoid Arthritis: History, Stages, Epidemiology, Pathogenesis, Diagnosis and Treatment

2017 ◽  
Vol 9 (02) ◽  
Author(s):  
Abeer Fauzi Al-Rubaye ◽  
Mohanad Jawad Kadhim ◽  
Imad Hadi Hameed
Keyword(s):  
Rheumatoid Arthritis ◽  
Medicinal Plants ◽  
Systemic Disease ◽  
Modern Medicine ◽  
Whole Body ◽  
Internal Organs ◽  
Synthetic Drugs ◽  
Relieve Pain ◽  
Medicinal Treatment ◽  
Natural Drugs

The pharmacological mechanisms of the medicinal plants traditionally used for RA in Persian medicine are discussed in the current review. Further investigations are mandatory to focus on bioefficacy of these phytochemicals for finding novel natural drugs. Rheumatoid arthritis is chronic, progressive, disabling autoimmune disease characterized by systemic inflammation of joints, damaging cartilage and bone around the joints. It is a systemic disease which means that it can affect the whole body and internal organs such as lungs, heart and eyes. Although numbers of synthetic drugs are being used as standard treatment for rheumatoid arthritis but they have adverse effect that can compromise the therapeutic treatment. Unfortunately, there is still no effective known medicinal treatment that cures rheumatoid arthritis as the modern medicine can only treat the symptoms of this disease that means to relieve pain and inflammation of joints. It is possible to use the herbs and plants in various forms in order to relieve the pain and inflammation in the joints. There are so many medicinal plants that have shown anti rheumatoid arthritis properties. So the plants and plant product with significant advantages are used for the treatment of rheumatoid arthritis. The present review is focused on the medicinal plants having anti rheumatoid arthritis activity

Pathomorphological changes in the internal organs of rats after chronic effect of chemical stress factors

2018 ◽  
Vol 3 (5) ◽  
pp. 63-66
Author(s):  
S. A. Sherstyuk ◽  
◽  
S. A. Nakonechnaya ◽  
E. V. Nakonechnyi ◽  
Е. E. Koschiy ◽  
...  
Keyword(s):  
Stress Factors ◽  
Chemical Stress ◽  
Chronic Effect ◽  
Internal Organs
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