Halobacterium vallismortis sp. nov. An amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium

1978 ◽  
Vol 24 (6) ◽  
pp. 710-715 ◽  
Author(s):  
Carmen Gonzalez ◽  
Carmen Gutierrez ◽  
C. Ramirez
Keyword(s):  
Type Strain ◽  
Halophilic Bacterium ◽  
Soluble Starch ◽  
Gas Production ◽  
Death Valley ◽  
Tween 20 ◽  
Facultative Anaerobe ◽  
Gram Negative ◽  
Optimum Growth Temperature ◽  
A New Species

The extremely halophilic bacterium (formerly designated as strain J.F. 54) isolated from salt pools of the Death Valley. California, is a motile, Gram-negative, extremely pleomorphic organism, aerobe, and facultative anaerobe. A variety of carbohydrates are assimilated with or without acid production; soluble starch is hydrolyzed. The organism is not proteolytic; catalase, oxidase, and DNase reactions are positive; Tween 20 is slightly hydrolyzed, but Tweens 40, 60, and 80 are not. Nitrates are reduced to nitrites with gas production; nitrites are not reduced. Optimum growth temperature is 40 °C. Growth is inhibited by bacitracin and by novobiocin. The type strain J.F. 54 differs from described species of the genus Halobacterium and is assigned to a new species, Halobacterium vallismortis sp. nov.

scholarly journals Haloargentinum marplatensis gen. nov., sp. nov., a novel extremely halophilic bacterium isolated from salted-ripened anchovy (Engraulis anchoita)

2020 ◽  
Author(s):  
Silvina Perez ◽  
Margarita Gomila ◽  
Silvia Elena Murialdo ◽  
Irene Mabel Ameztoy ◽  
Narjol Gonzalez-Escalona ◽  
...  
Keyword(s):  
Fatty Acids ◽  
16S Rrna ◽  
Type Strain ◽  
Close Relationships ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Gram Negative ◽  
Novel Genus And Species ◽  
Bacterium Strain ◽  
The 16S Rrna Gene

ABSTRACTA facultative aerobic, Gram-negative, motile, non-endospore forming and extremely halophilic bacterium, strain 11aii⊺, isolated from salted-ripened anchovy, was examined using a polyphasic approach to characterize and clarify its phylogenetic and taxonomic position. Sequences of the 16S rRNA gene revealed close relationships to species of the genera Lentibacillus and Virgibacillus (94.2% similarity). The organism grew optimally in the presence of 20-35 % NaCl. The major fatty acids of strain 11aii⊺ were C16:0 (42.1%) and anteiso-C15:0 (31.2%) and also presented iso-C16:0 (11.0%), anteiso-C17:0 (10.4%) and C18:0 (5.2%). Based on data presented here, strain 11aii⊺ is considered to represent a novel genus and species, for which the name Haloargentinum marplatensis gen. nov. sp. nov. is proposed with the strain 11aii⊺ as type strain.

scholarly journals Prolixibacter denitrificans sp. nov., an iron-corroding, facultatively aerobic, nitrate-reducing bacterium isolated from crude oil, and emended descriptions of the genus Prolixibacter and Prolixibacter bellariivorans

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 2865-2869 ◽  
Author(s):  
Takao Iino ◽  
Mitsuo Sakamoto ◽  
Moriya Ohkuma
Keyword(s):  
Growth Temperature ◽  
Nitrate Reduction ◽  
Type Strain ◽  
Novel Species ◽  
Soluble Starch ◽  
Phylogenetic Position ◽  
Optimum Growth ◽  
Glucose Fermentation ◽  
Optimum Growth Temperature ◽  
Growth Temperature Range

The facultatively aerobic, non-hydrogenotrophic, iron (Fe0)-corroding, nitrate-reducing Prolixibacter sp. strain MIC1-1T was characterized for representation of a novel species of the genus Prolixibacter. Strain MIC1-1T grew optimally at 35–37 °C, at pH 6.5 and with 2  % (w/v) NaCl. Strain MIC1-1T also grew fermentatively on some pentoses, hexoses, disaccharides and soluble starch. Succinic acid was the major end-product from d-glucose fermentation. Strain MIC1-1T was differentiated from the type strain of Prolixibacter bellariivorans by cell size, optimum growth temperature, range of temperature and NaCl for growth, and nitrate reduction. On the basis of phenotypic features and the phylogenetic position, a novel species of the genus Prolixibacter is proposed for strain MIC1-1T, to be named Prolixibacter denitrificans sp. nov. The type strain is MIC1-1T ( = JCM 18694T = NBRC 102688T = DSM 27267T). Emended descriptions of the genus Prolixibacter and Prolixibacter bellariivorans are also provided.

Bacillus oleroniussp.nov., a member of the hindgut flora of the termiteReticulitermes santonensis(Feytaud)

1995 ◽  
Vol 41 (8) ◽  
pp. 699-706 ◽  
Author(s):  
Thomas Kuhnigk ◽  
Eva-Maria Borst ◽  
Alfred Breunig ◽  
Helmut König ◽  
Matthew D. Collins ◽  
...  
Keyword(s):  
New Species ◽  
Type Strain ◽  
Staining Reaction ◽  
Bacillus Firmus ◽  
Genus Bacillus ◽  
16S Rrna Analysis ◽  
Gram Negative ◽  
Gram Staining ◽  
Reticulitermes Santonensis ◽  
A New Species

A new rod-shaped endospore-forming bacterium is described, which was isolated from the hindgut of the termite Reticulitermes santonensis (Feytaud). The isolate stains Gram negative and its DNA has a guanine-plus-cytosine content of 35 mol%. Despite the Gram-staining reaction, both biochemical and physiological features place the isolate in the genus Bacillus and indicate a phenotypic resemblance to the Bacillus firmus–lentus group of species. On the basis of comparative 16S rRNA analysis and some phenotypic features the isolate clearly represents a new species for which the name Bacillus oleronius is proposed. The type strain is Bacillus oleronius Rt 10 (DSM 9356).Key words: Bacillus, termites, hindgut flora.

scholarly journals Pseudidiomarina piscicola sp. nov., isolated from cultured European seabass, Dicenthrarchus labrax

2020 ◽  
Author(s):  
M. Carmen Macián ◽  
Teresa Lucena ◽  
David R. Arahal ◽  
María A. Ruvira ◽  
Rosa Aznar ◽  
...  
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Gram Negative ◽  
Cellular Fatty Acids ◽  
European Seabass ◽  
Total Salinity ◽  
A New Species ◽  
The 16S Rrna Gene

AbstractStrain CECT 9734 T, a Gram-negative, aerobic, chemoorganotrophic bacterium, motile by polar flagella, was isolated from cultured European seabass, Dicenthrarchus labrax, in Spain. It grows from 5 to 42 ºC, 6–9 pH and 1–12% total salinity. Major cellular fatty acids are C15:0 iso, summed feature 9 (C17:1 iso w9c/C16:0 10-methyl) and C17:0 iso. The genome size is 2.5 Mbp and G + C content is 49.5 mol%. Comparative analysis of the 16S rRNA gene sequence shows that the strain is a member of Pseudidiomarina, with highest similarities with Pseudidiomarina halophila (97.0%) and Pseudidiomarina salinarum (96.9%). Phylogenomic tree based on UBCG program shows P. halophila as its closest relative. ANI and in-silico DDH with other Pseudidiomarina spp. are lower than 87 and 20%, respectively, suggesting that strain CECT 9734 T represents a new species, for which we propose the name Pseudidiomarina piscicola sp. nov. and CECT 9734 T (= LUBLD50 7aT = LMG 31044 T) as type strain.

scholarly journals Geobacter bemidjiensis sp. nov. and Geobacter psychrophilus sp. nov., two novel Fe(III)-reducing subsurface isolates

2005 ◽  
Vol 55 (4) ◽  
pp. 1667-1674 ◽  
Author(s):  
Kelly P. Nevin ◽  
Dawn E. Holmes ◽  
Trevor L. Woodard ◽  
Erich S. Hinlein ◽  
David W. Ostendorf ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Electron Acceptors ◽  
Electron Donors ◽  
Amorphous Iron ◽  
Gram Negative ◽  
Subsurface Sediments ◽  
Curved Rods ◽  
A Site

Fe(III)-reducing isolates were recovered from two aquifers in which Fe(III) reduction is known to be important. Strain BemT was enriched from subsurface sediments collected in Bemidji, MN, USA, near a site where Fe(III) reduction is important in aromatic hydrocarbon degradation. Strains P11, P35T and P39 were isolated from the groundwater of an aquifer in Plymouth, MA, USA, in which Fe(III) reduction is important because of long-term inputs of acetate as a highway de-icing agent to the subsurface. All four isolates were Gram-negative, slightly curved rods that grew best in freshwater media. Strains P11, P35T and P39 exhibited motility via means of monotrichous flagella. Analysis of the 16S rRNA and nifD genes indicated that all four strains are δ-proteobacteria and members of the Geobacter cluster of the Geobacteraceae. Differences in phenotypic and phylogenetic characteristics indicated that the four isolates represent two novel species within the genus Geobacter. All of the isolates coupled the oxidation of acetate to the reduction of Fe(III) [iron(III) citrate, amorphous iron(III) oxide, iron(III) pyrophosphate and iron(III) nitrilotriacetate]. All four strains utilized ethanol, lactate, malate, pyruvate and succinate as electron donors and malate and fumarate as electron acceptors. Strain BemT grew fastest at 30 °C, whereas strains P11, P35T and P39 grew equally well at 17, 22 and 30 °C. In addition, strains P11, P35T and P39 were capable of growth at 4 °C. The names Geobacter bemidjiensis sp. nov. (type strain BemT=ATCC BAA-1014T=DSM 16622T=JCM 12645T) and Geobacter psychrophilus sp. nov. (strains P11, P35T and P39; type strain P35T=ATCC BAA-1013T=DSM 16674T=JCM 12644T) are proposed.

scholarly journals Proposal for the creation of a new genus Musicola gen. nov., reclassification of Dickeya paradisiaca (Samson et al. 2005) as Musicola paradisiaca comb. nov. and description of a new species Musicola keenii sp. nov.

2021 ◽  
Vol 71 (10) ◽  
Author(s):  
Nicole Hugouvieux-Cotte-Pattat ◽  
Cécile Jacot des-Combes ◽  
Jérôme Briolay ◽  
Leighton Pritchard
Keyword(s):  
Dna Hybridization ◽  
Type Strain ◽  
Type Species ◽  
Plant Pathogens ◽  
Phenotypic Analysis ◽  
Content Type ◽  
Link Type ◽  
Type Strains ◽  
A New Species ◽  
Laboratory Collection

The Pectobacteriaceae family of important plant pathogens includes the genus Dickeya . There are currently 12 described species of Dickeya , although some are poorly characterized at the genomic level. Only two genomes of Dickeya paradisiaca , the type strain CFBP 4178T and strain Ech703, have previously been sequenced. Members of this species are mostly of tropical or subtropical origin. During an investigation of strains present in our laboratory collection we sequenced the atypical strain A3967, registered as CFBP 722, isolated from Solanum lycopersicum (tomato) in the South of France in 1965. The genome of strain A3967 shares digital DNA–DNA hybridization and average nucleotide identity (ANI) values of 68 and 96 %, respectively, with the D. paradisiaca type strain CFBP 4178T. However, ANI analysis showed that D. paradisiaca strains are significantly dissimilar to the other Dickeya species, such that less than one third of their genomes align to any other Dickeya genome. On phenotypic, phylogenetic and genomic grounds, we propose a reassignment of D. paradisiaca to the genus level, for which we propose the name Musicola gen. nov., with Musicola paradisiaca as the type species and CFBP 4178T (NCPPB 2511T) as the type strain. Phenotypic analysis showed differences between strain A3967T and CFBP 4178T, such as for the assimilation of melibiose, raffinose and myo-inositol. These results support the description of two novel species, namely Musicola paradisiaca comb. nov. and Musicola keenii sp. nov., with CFBP 4178T (NCPPB 2511T=LMG 2542T) and A3967T (CFBP 8732T=LMG 31880T) as the type strains, respectively.

scholarly journals Proposal of Thermoactinomyces mirandus sp. nov., a filamentous, anaerobic bacterium isolated from a biogas plant

2020 ◽  
Author(s):  
Mira Mutschlechner ◽  
Nina Lackner ◽  
Rudolf Markt ◽  
Willi Salvenmoser ◽  
Christopher A. Dunlap ◽  
...  
Keyword(s):  
Sequence Comparison ◽  
Type Strain ◽  
Gene Sequence ◽  
Genetic Relatedness ◽  
Biogas Plant ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Optimum Growth Temperature ◽  
Nacl Concentration ◽  
Thermoactinomyces Vulgaris

AbstractWe isolated a filamentous, thermophilic, and first anaerobic representative of the genus Thermoactinomyces, designated strain AMNI-1T, from a biogas plant in Tyrol, Austria and report the results of a phenotypic, genetic, and phylogenetic investigation. Strain AMNI-1T was observed to form a white branching mycelium that aggregates into pellets when grown in liquid medium. Cells could primarily utilize lactose, glucose, and mannose as carbon and energy sources, with acetate accelerating and yeast extract being mandatory for growth. The optimum growth temperature and pH turned out to be 55 °C and pH 7.0, respectively, with an optimum NaCl concentration of 0–2% (w/v). 16S rRNA gene sequence comparison indicated that the genetic relatedness between strain AMNI-1T and Thermoactinomyces intermedius, Thermoactinomyces khenchelensis, and Thermoactinomyces vulgaris was less than 97%. The G + C content of the genomic DNA was 44.7 mol%. The data obtained suggest that the isolate represents a novel and first anaerobic species of the genus Thermoactinomyces, for which the name Thermoactinomyces mirandus is proposed. The type strain is AMNI-1T (= DSM 110094T = LMG 31503T). The description of the genus Thermoactinomyces is emended accordingly.

The complete structure of the core carbohydrate backbone from the LPS of marine halophilic bacterium Pseudoalteromonas carrageenovora type strain IAM 12662T

2005 ◽  
Vol 340 (8) ◽  
pp. 1475-1482 ◽  
Author(s):  
Alba Silipo ◽  
Rosa Lanzetta ◽  
Michelangelo Parrilli ◽  
Luisa Sturiale ◽  
Domenico Garozzo ◽  
...  
Keyword(s):  
Type Strain ◽  
Halophilic Bacterium ◽  
Complete Structure ◽  
The Core

scholarly journals A New Chemolithoautotrophic Arsenite-Oxidizing Bacterium Isolated from a Gold Mine: Phylogenetic, Physiological, and Preliminary Biochemical Studies

2000 ◽  
Vol 66 (1) ◽  
pp. 92-97 ◽  
Author(s):  
Joanne M. Santini ◽  
Lindsay I. Sly ◽  
Roger D. Schnagl ◽  
Joan M. Macy
Keyword(s):  
Carbon Dioxide ◽  
New Species ◽  
Minimal Medium ◽  
Doubling Time ◽  
Northern Territory ◽  
Gold Mine ◽  
Gram Negative ◽  
Biochemical Studies ◽  
Optimum Ph ◽  
A New Species

ABSTRACT A previously unknown chemolithoautotrophic arsenite-oxidizing bacterium has been isolated from a gold mine in the Northern Territory of Australia. The organism, designated NT-26, was found to be a gram-negative motile rod with two subterminal flagella. In a minimal medium containing only arsenite as the electron donor (5 mM), oxygen as the electron acceptor, and carbon dioxide-bicarbonate as the carbon source, the doubling time for chemolithoautotrophic growth was 7.6 h. Arsenite oxidation was found to be catalyzed by a periplasmic arsenite oxidase (optimum pH, 5.5). Based upon 16S rDNA phylogenetic sequence analysis, NT-26 belongs to theAgrobacterium/Rhizobium branch of the α-Proteobacteria and may represent a new species. This recently discovered organism is the most rapidly growing chemolithoautotrophic arsenite oxidizer known.

scholarly journals Roseospira visakhapatnamensis sp. nov. and Roseospira goensis sp. nov.

2007 ◽  
Vol 57 (11) ◽  
pp. 2453-2457 ◽  
Author(s):  
S. Kalyan Chakravarthy ◽  
T. N. R. Srinivas ◽  
P. Anil Kumar ◽  
Ch. Sasikala ◽  
Ch. V. Ramana
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Aminobenzoic Acid ◽  
Rrna Gene Sequence ◽  
Gram Negative ◽  
Photosynthetic Membranes ◽  
Marine Habitats ◽  
Gene Sequence Analysis

Two Gram-negative, vibrioid, phototrophic, purple non-sulfur strains, JA131T and JA135T, were isolated from marine habitats. Strain JA131T is non-motile but strain JA135T is motile by means of a pair of monopolar flagella. Both strains have an obligate requirement for NaCl for growth. The intracellular photosynthetic membranes of the two novel strains are of the vesicular type. Bacteriochlorophyll a and probably rhodovibrine are present as photosynthetic pigments. Niacin, thiamine and p-aminobenzoic acid are required as growth factors for both novel strains. Based on 16S rRNA gene sequence analysis, morphological and physiological characteristics, strains JA131T and JA135T are significantly different from each other and from other species of the genus Roseospira and thus represent two novel species for which the names Roseospira visakhapatnamensis sp. nov. and Roseospira goensis sp. nov. are proposed, respectively. The type strain of Roseospira visakhapatnamensis sp. nov. is JA131T (=ATCC BAA-1365T=JCM 14190T) and the type strain of Roseospira goensis sp. nov. is JA135T (=ATCC BAA-1364T=JCM 14191T).

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