New Minto virus: a new rhabdovirus from ticks in Alaska

1978 ◽  
Vol 24 (4) ◽  
pp. 422-426 ◽  
Author(s):  
Donald G. Ritter ◽  
Charles H. Calisher ◽  
David J. Muth ◽  
Robert E. Shope ◽  
Frederick A. Murphy ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Complement Fixation ◽  
Sodium Deoxycholate ◽  
Lepus Americanus ◽  
Pekin Duck ◽  
East Central ◽  
Snowshoe Hares ◽  
The Family ◽  
Subcutaneous Inoculation ◽  
Family Rhabdoviridae

Three strains of a virus were isolated from Haemaphysalis leporis-palustris (Packard) ticks removed from snowshoe hares (Lepus americanus Erxleben) in east central Alaska. We suggest that the virus be named New Minto for the location in which the ticks were collected. Prototype New Minto virus is sensitive to the action of sodium deoxycholate and kills suckling mice by the intracerebral but not intraperitoneal route; weaned mice do not die after intracerebral, intraperitoneal, or subcutaneous inoculation. The virus produces plaque in serially propagated Vero but not in primary Pekin duck embryo cells.By complement-fixation and neutralization tests New Minto is related to Sawgrass virus, a hitherto ungrouped virus from Florida. The establishment of a Sawgrass group is suggested. In addition, Sawgrass virus was found by electron microscopy to belong to the Family Rhabdoviridae.

Immune Electron Microscopy (IEM) of a Canine Adeno-Associated Virus

1974 ◽  
Vol 32 ◽  
pp. 256-257
Author(s):  
Gunter F. Thomas ◽  
M. David Hoggan
Keyword(s):  
Electron Microscopy ◽  
Cell Cultures ◽  
Complement Fixation ◽  
Hepatitis Virus ◽  
Wide Distribution ◽  
Immune Electron Microscopy ◽  
Adeno Associated Virus ◽  
Virus Like Particle ◽  
Dog Kidney ◽  
Green Monkeys

In 1968, Sugimura and Yanagawa described a small 25 nm virus like particle in association with the Matsuda strain of infectious canine hepatitis virus (ICHV). Domoto and Yanagawa showed that this particle was dependent on ICHV for its replication in primary dog kidney cell cultures (PDK) and was resistant to heating at 70°C for 10 min, and concluded that it was a canine adeno-associated virus (CAAV). Later studies by Onuma and Yanagawa compared CAAV with the known human serotypes (AAV 1, 2, 3) and AAV-4, known to be associated with African Green Monkeys. Using the complement fixation (CF) test, they found that CAAV was serologically related to AAV-3 and had wide distribution in the dog population of Japan.

Electron Microscopy of Mycoplasma Pneumoniae

1971 ◽  
Vol 29 ◽  
pp. 258-259 ◽  
Author(s):  
E. S. Boatman ◽  
G. E. Kenny
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Growth Phase ◽  
Hela Cells ◽  
Sulfonic Acid ◽  
Gamma Globulin ◽  
Horse Serum ◽  
Morphological Aspects ◽  
The Family

Information concerning the morphology and replication of organism of the family Mycoplasmataceae remains, despite over 70 years of study, highly controversial. Due to their small size observations by light microscopy have not been rewarding. Furthermore, not only are these organisms extremely pleomorphic but their morphology also changes according to growth phase. This study deals with the morphological aspects of M. pneumoniae strain 3546 in relation to growth, interaction with HeLa cells and possible mechanisms of replication.The organisms were grown aerobically at 37°C in a soy peptone yeast dialysate medium supplemented with 12% gamma-globulin free horse serum. The medium was buffered at pH 7.3 with TES [N-tris (hyroxymethyl) methyl-2-aminoethane sulfonic acid] at 10mM concentration. The inoculum, an actively growing culture, was filtered through a 0.5 μm polycarbonate “nuclepore” filter to prevent transfer of all but the smallest aggregates. Growth was assessed at specific periods by colony counts and 800 ml samples of organisms were fixed in situ with 2.5% glutaraldehyde for 3 hrs. at 4°C. Washed cells for sectioning were post-fixed in 0.8% OSO4 in veronal-acetate buffer pH 6.1 for 1 hr. at 21°C. HeLa cells were infected with a filtered inoculum of M. pneumoniae and incubated for 9 days in Leighton tubes with coverslips. The cells were then removed and processed for electron microscopy.

scholarly journals Pollen, Tapetum, and Orbicule Development inColletia paradoxaandDiscaria americana(Rhamnaceae)

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
M. Gotelli ◽  
B. Galati ◽  
D. Medan
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Ultrastructural Changes ◽  
Transmission Electron ◽  
The Family ◽  
Tapetal Cells ◽  
Grain Formation

Tapetum, orbicule, and pollen grain ontogeny inColletia paradoxaandDiscaria americanawere studied with transmission electron microscopy (TEM). The ultrastructural changes observed during the different stages of development in the tapetal cells and related to orbicule and pollen grain formation are described. The proorbicules have the appearance of lipid globule, and their formation is related to the endoplasmic reticulum of rough type (ERr). This is the first report on the presence of orbicules in the family Rhamnaceae. Pollen grains are shed at the bicellular stage.

Ascospore sculpturing and generic concepts in the Testudinaceae (syn. Zopfiaceae)

1979 ◽  
Vol 57 (2) ◽  
pp. 91-99 ◽  
Author(s):  
D. L. Hawksworth
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Combination ◽  
The Family ◽  
First Time ◽  
Scanning Electron

Generic concepts in the Testudinaceae (Ascomycotina, Loculoascomycetes) are reviewed with particular emphasis on the sculpturing of the ascospores which has been examined by scanning electron microscopy (SEM) for the first time. A key to the nine genera accepted in the family is provided of which two are described as new: Ulospora for U. bilgramii comb.nov. (= Zopfia bilgramii D. Hawksw. et al.), the ascospores of which have three to six deep fissures in each cell, and Zopfiofoveola for Z. punctata comb.nov. (= Zopfia punctata D. Hawksw. & C. Booth), with distinctly and regularly foveolate ascospores. One further new combination is made: Rechingeriella boudieri comb.nov. (= Zopfia boudieri Arnaud). The positions of three additional genera referred to the family by earlier authors but which are excluded from it here are also briefly discussed.

New perspectives on Nephridiacanthus major (Acanthocephala: Oligacanthorhynchidae) collected from hedgehogs in Iran

2020 ◽  
Vol 94 ◽  
Author(s):  
O.M. Amin ◽  
M. Sharifdini ◽  
R.A. Heckmann ◽  
M. Zarean
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Analysis ◽  
Chemical Analysis ◽  
Central Asia ◽  
Molecular Profile ◽  
X Ray ◽  
The Family ◽  
First Time ◽  
Scanning Electron

We describe morphological features not previously reported for this old acanthocephalan Nephridiacanthus major (Bremser, 1811 in Westrumb, 1821) Golvan, 1962 first described over 200 years ago. Our specimens were collected from long-eared hedgehog Hemiechinus auritus (Gmelin, 1770) (Erinaceidae) in Iran. We compare the morphometrics of our material with others previously reported from the same host in Iran, Russia, central Asia and Europe. Our specimens had markedly smaller proboscides, proboscis hooks and lemnisci than those reported from Russia and central Asia, but comparable measurements of other structures with specimens previously described from other collections. We document our new observations with scanning electron microscopy features not previously demonstrable by other observers and provide a chemical analysis of proboscis hooks using energy-dispersive X-ray analysis for the first time. The molecular profile of this acanthocephalan, based on 18S rDNA and cox1 genes, was generated for the first time. The phylogenetic analysis showed that N. major is placed in a clade of the family Oligacanthorhynchidae, well separated from the families Moniliformidae and Gigantorhynchidae.

Dynamics of a snow shoe hare population in fragmented habitat

1993 ◽  
Vol 71 (7) ◽  
pp. 1385-1392 ◽  
Author(s):  
Lloyd B. Keith ◽  
Sara E. M. Bloomer ◽  
Tomas Willebrand
Keyword(s):  
Patch Size ◽  
Canis Latrans ◽  
Lepus Americanus ◽  
Fragmented Habitat ◽  
Proximate Cause ◽  
Lower Survival ◽  
The Third ◽  
Snowshoe Hares ◽  
Poorly Drained Soils ◽  
Hare Population

During November 1988 – December 1991 we livetrapped, radio-collared, and monitored the survival, reproduction, and movements of snowshoe hares (Lepus americanus) in highly fragmented habitat near the species' geographic limit in central Wisconsin. Our 7 study areas centered on 5- to 28-ha patches of prime habitat: dense stands of willow (Salix), alder (Alnus), and regenerating aspen (Populus) on poorly drained soils. Maximum hare densities averaged 1.6 – 0.8/ha, and were unrelated to patch size. Rapid declines to extinction occurred on 3 of the 5 smallest study areas; on another, where extinction seemed imminent, juvenile ingress restored the population. On the 2 largest areas (23 – 28 ha of prime habitat) hare populations were stationary during the first 2 years, but declined by 50 – 70% in the third as mean annual (September – August) survival of radio-collared hares fell from 0.27 (1988 – 1990) to 0.07 (1990 – 1991). Annual survival on the 3 extinction sites averaged just 0.015 compared with 0.179 elsewhere. Reproduction did not differ between small (5 – 7 ha) vs. larger (23 – 28 ha) patches nor between years. Estimated dispersal of adult and juvenile hares from the 5 small study areas was twice as high as from the 2 larger, viz. 16 vs. 35% annually. Dispersers appeared to have markedly lower survival. Predation, chiefly by coyotes (Canis latrans), was the proximate cause of 96% (117 of 122) of natural deaths among radio-collared hares, and was therefore the overwhelming determinant of survival and thus population trend. Results of this study suggest that probabilities of extinction in such fragmented habitat depend importantly on patch size and attendant hare numbers; i.e., fall populations of < 10 hares frequenting patches of prime habitat ≤ 5 ha are not likely to persist long without ingress.

scholarly journals Fine Structure of Maxillary Palps in Adults of Hermetia illucens (Diptera: Stratiomyidae)

2020 ◽  
Author(s):  
M Pezzi ◽  
C Scapoli ◽  
M Bharti ◽  
M J Faucheux ◽  
M Chicca ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sexual Dimorphism ◽  
Hermetia Illucens ◽  
The Family ◽  
Sensory Structures ◽  
First Time ◽  
Maxillary Palps ◽  
Scanning Electron

Abstract A relevant species in waste management but also in forensic, medical, and veterinary sciences is the black soldier fly, Hermetia illucens (Linnaeus; Diptera: Stratiomyidae). An ultrastructural study by scanning electron microscopy (SEM) was conducted for the first time on maxillary palps of both sexes, describing in detail the morphology and distribution of sensilla and microtrichia. The maxillary palps, composed of two segments, show sexual dimorphism in length and shape. In both sexes, the first segment is covered only by microtrichia, but the second one is divided into two parts: the proximal one, covered only by microtrichia, and the distal one containing both microtrichia and sensory structures. These structures include two types of sensory pits and one of chaetic sensilla. Due to sexual dimorphism in palp size, females have a higher number of sensory pits. The sexual dimorphism of palps and the presence and role of sensilla in H. illucens was discussed in comparison to other species of the family Stratiomyidae and of other Diptera. This study may represent a base for further investigations on mouthpart structures of this species, involved in key physiological activities, such as feeding, mating and oviposition.

scholarly journals Al5+αSi5+δN12, a new Nitride compound

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
R. Dagher ◽  
L. Lymperakis ◽  
V. Delaye ◽  
L. Largeau ◽  
A. Michon ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Density Functional ◽  
Cation Sublattice ◽  
Nitride Semiconductors ◽  
Anion Sublattice ◽  
Functional Theory ◽  
X Ray ◽  
Transmission Electron ◽  
The Family ◽  
Heteroepitaxial Layers

Abstract The family of III-Nitride semiconductors has been under intensive research for almost 30 years and has revolutionized lighting applications at the dawn of the 21st century. However, besides the developments and applications achieved, nitride alloys continue to fuel the quest for novel materials and applications. We report on the synthesis of a new nitride-based compound by using annealing of AlN heteroepitaxial layers under a Si-atmosphere at temperatures between 1350 °C and 1550 °C. The structure and stoichiometry of this compound are investigated by high resolution transmission electron microscopy (TEM) techniques and energy dispersive X-Ray (EDX) spectroscopy. Results are supported by density functional theory (DFT) calculations. The identified structure is a derivative of the parent wurtzite AlN crystal where the anion sublattice is fully occupied by N atoms and the cation sublattice is the stacking of 2 different planes along <0001>: The first one exhibits a ×3 periodicity along <11–20> with 1/3 of the sites being vacant. The rest of the sites in the cation sublattice are occupied by an equal number of Si and Al atoms. Assuming a semiconducting alloy, a range of stoichiometries is proposed, Al5+αSi5+δN12 with α being between −2/3 and 1/4 and δ between 0 and 3/4.

Sign in / Sign up

Export Citation Format

Share Document