Breakdown of psyllium hydrocolloid by strains of Bacteroides ovatus from the human intestinal tract

1978 ◽  
Vol 24 (3) ◽  
pp. 336-338 ◽  
Author(s):  
Abigail A. Salyers ◽  
Charlotte J. Harris ◽  
Tracy D. Wilkins
Keyword(s):  
Intestinal Tract ◽  
Extracellular Enzymes ◽  
Human Feces ◽  
Bacteroides Ovatus ◽  
High Concentrations ◽  
Human Intestinal Tract

Strains of Bacteroides ovatus from human feces produced extracellular enzymes which degraded psyllium hydrocolloid. Other species of saccharolytic bacteria which are found in high concentrations in human feces did not degrade psyllium.

scholarly journals Distribution of Bifidobacterial Species in Human Intestinal Microflora Examined with 16S rRNA-Gene-Targeted Species-Specific Primers

1999 ◽  
Vol 65 (10) ◽  
pp. 4506-4512 ◽  
Author(s):  
Takahiro Matsuki ◽  
Koichi Watanabe ◽  
Ryuichiro Tanaka ◽  
Masafumi Fukuda ◽  
Hiroshi Oyaizu
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Intestinal Tract ◽  
Bifidobacterium Longum ◽  
Rrna Gene ◽  
Human Feces ◽  
Specific Primers ◽  
Specific Pcr ◽  
Species Specific ◽  
Human Intestinal Tract

ABSTRACT In order to clarify the distribution of bifidobacterial species in the human intestinal tract, a 16S rRNA-gene-targeted species-specific PCR technique was developed and used with DNAs extracted from fecal samples obtained from 48 healthy adults and 27 breast-fed infants. To cover all of the bifidobacterial species that have been isolated from and identified in the human intestinal tract, species-specific primers for Bifidobacterium longum, B. infantis,B. dentium, and B. gallicum were developed and used with primers for B. adolescentis, B. angulatum, B. bifidum, B. breve, and the B. catenulatum group (B. catenulatum andB. pseudocatenulatum) that were developed in a previous study (T. Matsuki, K. Watanabe, R. Tanaka, and H. Oyaizu, FEMS Microbiol. Lett. 167:113–121, 1998). The specificity of the nine primers was confirmed by PCR, and the species-specific PCR method was found to be a useful means for identifying Bifidobacteriumstrains isolated from human feces. The results of an examination of bifidobacterial species distribution showed that the B. catenulatum group was the most commonly found taxon (detected in 44 of 48 samples [92%]), followed by B. longum andB. adolescentis, in the adult intestinal bifidobacterial flora and that B. breve, B. infantis, andB. longum were frequently found in the intestinal tracts of infants. The present study demonstrated that qualitative detection of the bifidobacterial species present in human feces can be accomplished rapidly and accurately.

scholarly journals Recovery of SARS-CoV-2 from Wastewater Using Centrifugal Ultrafiltration

2021 ◽  
Vol 4 (2) ◽  
pp. 32
Author(s):  
Brienna L. Anderson-Coughlin ◽  
Adrienne E. H. Shearer ◽  
Alexis N. Omar ◽  
K. Eric Wommack ◽  
Kalmia E. Kniel
Keyword(s):  
Enteric Virus ◽  
Clinical Testing ◽  
Human Feces ◽  
Angiotensin Converting Enzyme 2 ◽  
Human Waste ◽  
Safety Research ◽  
New Castle County ◽  
Wastewater Systems ◽  
High Concentrations ◽  
Centrifugal Ultrafiltration

The COVID-19 pandemic is a global crisis and continues to impact communities as the disease spreads. Clinical testing alone provides a snapshot of infected individuals but is costly and difficult to perform logistically across whole populations. The virus which causes COVID-19, SARS-CoV-2, is shed in human feces and urine and can be detected in human waste. SARS-CoV-2 can be shed in high concentrations (>107 genomic copies/mL) due to its ability to replicate in the gastrointestinal tract of humans through attachment to the angiotensin-converting enzyme 2 (ACE-2) receptors there. Monitoring wastewater for SARS-CoV-2, alongside clinical testing, can more accurately represent the spread of disease within a community. This protocol describes a reliable and efficacious method to recover SARS-CoV-2 in wastewater, quantify genomic RNA levels, and evaluate concentration fluctuations over time. Using this protocol, viral levels as low as 10 genomic copies/mL were successfully detected from 30 mL of wastewater in more than seven-hundred samples collected between August 2020 and March 2021. Through the adaptation of traditional enteric virus methods used in food safety research, targets have been reliably detected with no inhibition of detection (RT-qPCR) observed in any sample processed. This protocol is currently used for surveillance of wastewater systems across New Castle County, Delaware.

The human intestinal tract – a hotbed of resistance gene transfer? Part I

2007 ◽  
Vol 29 (3) ◽  
pp. 17-21 ◽  
Author(s):  
Abigail A. Salyers ◽  
Kyung Moon ◽  
David Schlesinger
Keyword(s):  
Gene Transfer ◽  
Resistance Gene ◽  
Intestinal Tract ◽  
Human Intestinal Tract

Enterotoxin gene content inStaphylococcus aureusfrom the human intestinal tract

2009 ◽  
Vol 296 (1) ◽  
pp. 72-77 ◽  
Author(s):  
Elżbieta Lis ◽  
Kamila Korzekwa ◽  
JarosÅ‚aw BystroÅ„ ◽  
Agnieszka Å»arczyÅ„ska ◽  
Anna DÄ…browska ◽  
...  
Keyword(s):  
Intestinal Tract ◽  
Gene Content ◽  
Enterotoxin Gene ◽  
Human Intestinal Tract

scholarly journals Monitoring and Survival ofLactobacillus gasseriSBT2055 in the Human Intestinal Tract

2006 ◽  
Vol 50 (11) ◽  
pp. 867-870 ◽  
Author(s):  
Hidetoshi Takahashi ◽  
Takashi Fujita ◽  
Yutaka Suzuki ◽  
Yoshimi Benno
Keyword(s):  
Intestinal Tract ◽  
Human Intestinal Tract
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