Accessory pigment fluorescence for quantitation of photosynthetic microbial populations

1977 ◽  
Vol 23 (11) ◽  
pp. 1594-1597 ◽  
Author(s):  
Douglas E. Caldwell

The in vivo fluorescence of the primary accessory pigments in purple bacteria (carotenoids), green bacteria (bacteriochlorophyll), green algae (chlorophyll), and cyanobacteria (phycocyanin) was found to be a linear function of cell concentration over three to four orders of magnitude. The lowest cell concentrations detectable were 104 cells/ml for procaryotes and 103 cells/ml for eucaryotes.

1993 ◽  
Vol 28 (6) ◽  
pp. 29-33 ◽  
Author(s):  
V. Vyhnálek ◽  
Z. Fišar ◽  
A. Fišarová ◽  
J. Komárková

The in vivo fluorescence of chlorophyll a was measured in samples of natural phytoplankton taken from the Římov Reservoir (Czech Republic) during the years 1987 and 1988. The fluorescence intensities of samples either with or without addition of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (diuron, DCMU) were found reliable for calculating the concentration of chlorophyll a during periods when cyanobacteria were not abundant. The correction for background non-chlorophyll fluorescence appeared to be essential. No distinct correlation between a DCMU-induced increase of the fluorescence and primary production of phytoplankton was found.


2017 ◽  
Vol 262 ◽  
pp. 185-188 ◽  
Author(s):  
Alison Cox ◽  
Christopher G. Bryan

Previous agglomerate-scale heap bioleaching studies have outlined the variations in cell numbers of the liquid and attached phases during colonisation of sterilised ore by a pure culture. In this study, a mixed mesophilic culture was used in agglomerate-scale columns containing non-sterilised low-grade copper ore. Over a six - month period, columns were harvested at various intervals to provide snapshots of the metal distribution and the quantity, location, and ecological variations of mineral-oxidizing microbes within the ore bed. The initial colonisation period in this experiment was dissimilar to previous work, as the indigenous community was retained within the ore-bed throughout acid agglomeration. The overall colonisation phase lasted for approximately 1,000 hours until cell concentrations stabilised. In each column, less than 0.05% of the total cells were found in the leachate, 15-20% in the interstitial phase and the remaining ~80% were attached to the mineral surface. Once cell numbers had stabilised, interstitial cell concentrations were approximately 2,000× greater than those in the leachate. This difference persisted for the duration of the experiment. Copper concentrations in the two liquid phases generally decreased over time, but were on average 50× higher in the interstitial phase. Iron concentrations were more stable, but again were 30× higher in the interstitial phase. This demonstrates that that the difference in cell concentration between the leachate and interstitial phases cannot be explained through diffusion gradients within the system as it is much greater than those observed for the dissolved metals. It also shows that the specific environmental conditions of the interstitial and attached cells are very different to those inferred through analysis of leachates alone.


2011 ◽  
Vol 16 (9) ◽  
pp. 096013 ◽  
Author(s):  
Julien Gravier ◽  
Fabrice P. Navarro ◽  
Thomas Delmas ◽  
Frédérique Mittler ◽  
Anne-Claude Couffin ◽  
...  

2010 ◽  
Vol 68 ◽  
pp. e155
Author(s):  
Hiroshi Sekiya ◽  
Shigeyuki Namiki ◽  
Hirokazu Sakamoto ◽  
Sho Iinuma ◽  
Kenzo Hirose ◽  
...  

2014 ◽  
Vol 20 (13) ◽  
pp. 3531-3539 ◽  
Author(s):  
Yasaman Ardeshirpour ◽  
Victor Chernomordik ◽  
Moinuddin Hassan ◽  
Rafal Zielinski ◽  
Jacek Capala ◽  
...  

2012 ◽  
Vol 51 (39) ◽  
pp. 9818-9821 ◽  
Author(s):  
Guosong Hong ◽  
Joshua T. Robinson ◽  
Yejun Zhang ◽  
Shuo Diao ◽  
Alexander L. Antaris ◽  
...  

2011 ◽  
Vol 27 (6) ◽  
pp. 575-583 ◽  
Author(s):  
Sevim Kahraman ◽  
Ercument Dirice ◽  
Fatma Z. Hapil ◽  
Mustafa G. Ertosun ◽  
Saffet Ozturk ◽  
...  

1997 ◽  
Vol 15 (1) ◽  
pp. 55-58 ◽  
Author(s):  
Yoshinori Takema ◽  
Yukiko Yorimoto ◽  
Hiroyuki Ohsu ◽  
Osamu Osanai ◽  
Michio Kawai

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