Characterization of Streptococcus bovis bacteriophages

1976 ◽  
Vol 22 (6) ◽  
pp. 847-852 ◽  
Author(s):  
W. G. Iverson ◽  
Nancy F. Millis
Keyword(s):  
Growth Curve ◽  
Rumen Fluid ◽  
Single Step ◽  
Antigenic Relationship ◽  
Streptococcus Bovis ◽  
Serum Neutralization ◽  
Phage Types ◽  
Step Growth ◽  
Group D

About 25 Streptococcus bovis bacteriophages were isolated from abattoir wastes, bovine rumen fluid, and lysogenic strains of S. bovis. Eight phages were selected and characterized by morphology, stability, rate of adsorption, single-step growth curve, serum neutralization, and antigenic relationship. Two distinct morphological phage types were found, one of which has not been previously reported for group D streptococci.

scholarly journals Characterization of RNA synthesis during a one-step growth curve and of the replication mechanism of bovine viral diarrhoea virus

1996 ◽  
Vol 77 (11) ◽  
pp. 2729-2736 ◽  
Author(s):  
Y. Gong ◽  
R. Trowbridge ◽  
T. B. Macnaughton ◽  
E. G. Westaway ◽  
A. D. Shannon ◽  
...  
Keyword(s):  
Growth Curve ◽  
Rna Synthesis ◽  
Bovine Viral Diarrhoea Virus ◽  
Bovine Viral Diarrhoea ◽  
Replication Mechanism ◽  
Diarrhoea Virus ◽  
One Step ◽  
Step Growth

scholarly journals Characterization of Six Bacteriophages ofSerratia liquefaciens CP6 Isolated from the Sugar Beet Phytosphere

1999 ◽  
Vol 65 (5) ◽  
pp. 1959-1965 ◽  
Author(s):  
Kevin E. Ashelford ◽  
John C. Fry ◽  
Mark J. Bailey ◽  
Aaron R. Jeffries ◽  
Martin J. Day
Keyword(s):  
Sugar Beet ◽  
Burst Size ◽  
Single Step ◽  
The Other ◽  
Cross Hybridization ◽  
The Family ◽  
Step Growth ◽  
Natural Ecology ◽  
Changes Over Time

ABSTRACT Six phages (ΦCP6-1 to ΦCP6-6) that are commonly found in the phytosphere of sugar beet (Beta vulgaris var. Amethyst) were investigated, and their relative impacts on their host (Serratia liquefaciens CP6) were compared. There were fundamental differences between the two most abundant predators of CP6 (ΦCP6-1 and ΦCP6-4). Like ΦCP6-2 and ΦCP6-5, ΦCP6-1 belonged to the family Siphoviridae, while ΦCP6-4 exhibited the morphology of the family Podoviridae. The other phages were members of the family Myoviridae. DNA-DNA cross-hybridization revealed that ΦCP6-1 and ΦCP6-4 had little common DNA, although all of the other phages exhibited some genetic similarity. Like ΦCP6-2, ΦCP6-3, and ΦCP6-5, ΦCP6-1 was capable of forming a lysogenic association with its host, while ΦCP6-4 and ΦCP6-6 appeared to be entirely virulent. Single-step growth curve experiments revealed that ΦCP6-4 had a much shorter latent period and a smaller burst size than ΦCP6-1. Also, ΦCP6-1 could transduce a number of host chromosomal markers with transfer frequencies of 2.9 × 10−9 to 3.9 × 10−7, whereas ΦCP6-4 could not transduce S. liquefaciens CP6 genes. When viewed in the context of the strikingly different temporal niches of these phages, our data provide an insight into how bacteriophage interactions with their hosts might reflect the natural ecology of bacteriophages. Our data also illustrate how the potential for gene transfer changes over time in an environment that supports several different phages.

Characterization of Streptococcus durans bacteriophages

1968 ◽  
Vol 14 (4) ◽  
pp. 397-402 ◽  
Author(s):  
Marvin D. Brailsford ◽  
Paul A. Hartman
Keyword(s):  
Adsorption Kinetics ◽  
Temperature Effects ◽  
Rumen Fluid ◽  
Growth Characteristics ◽  
Plaque Morphology ◽  
Fecal Samples ◽  
Bovine Rumen ◽  
One Step ◽  
Step Growth

Streptococcus durans bacteriophages were isolated from bovine rumen fluid, feed lot soil, bovine fecal samples, and lysogenic strains of S. durans. The presence of different phage strains was suggested by antiserum neutralization tests and one-step growth characteristics, whereby the bacteriophages were placed into three distinct, but serologically related, groups. These phages were further characterized by plaque morphology, phage morphology, adsorption kinetics, and pH and temperature effects.

scholarly journals Characterization of Poliovirus Variants Selected for Resistance to the Antiviral Compound V-073

2012 ◽  
Vol 56 (11) ◽  
pp. 5568-5574 ◽  
Author(s):  
Hong-Mei Liu ◽  
Jason A. Roberts ◽  
Deborah Moore ◽  
Barbara Anderson ◽  
Mark A. Pallansch ◽  
...  
Keyword(s):  
Single Step ◽  
Single Amino Acid ◽  
Drug Resistant ◽  
Antiviral Compound ◽  
Equivalent Position ◽  
Single Amino Acid Change ◽  
Step Growth ◽  
Type 3

ABSTRACTV-073, a small-molecule capsid inhibitor originally developed for nonpolio enterovirus indications is considerably more potent against polioviruses. All poliovirus isolates tested to date (n= 45), including wild, vaccine, vaccine-derived, and laboratory strains, are susceptible to the antiviral capsid inhibitor V-073. We grew poliovirus in the presence of V-073 to allow for the identification of variants with reduced susceptibility to the drug. Sequence analysis of 160 independent resistant variants (80 isolates of poliovirus type 1, 40 isolates each of types 2 and 3) established that V-073 resistance involved a single amino acid change in either of two virus capsid proteins, VP1 (67 of 160 [42%]) or VP3 (93 of 160 [58%]). In resistant variants with a VP1 change, the majority (53 of 67 [79%]) exhibited a substitution of isoleucine at position 194 (equivalent position 192 in type 3) with either methionine or phenylalanine. Of those with a VP3 change, alanine at position 24 was replaced with valine in all variants (n= 93). The resistance phenotype was relatively stable upon passage of viruses in cell culture in the absence of drug. Single-step growth studies showed no substantial differences between drug-resistant variants and the virus stocks from which they were derived, while the resistant viruses were generally more thermally labile than the corresponding drug-susceptible parental viruses. These studies provide a foundation from which to build a greater understanding of resistance to antiviral compound V-073.

Isolation and Characterization of a Rape MAP Kinase BnMPK9 from Group D

2013 ◽  
Vol 39 (3) ◽  
pp. 440
Author(s):  
Shun-Wu YU ◽  
Li-Da ZHANG ◽  
Guo-Lan LIU ◽  
Li-Jun LUO
Keyword(s):  
Map Kinase ◽  
Isolation And Characterization ◽  
Group D

Polymer Chemistry

2004 ◽  
Keyword(s):  
Conducting Polymers ◽  
Liquid Crystalline ◽  
Polymer Chemistry ◽  
Chain Growth ◽  
Crystalline Polymers ◽  
Wide Range ◽  
Cyclic Oligomers ◽  
Step Growth

Polymer Chemistry: A Practical Approach in Chemistry has been designed for both chemists working in and new to the area of polymer synthesis. It contains detailed instructions for preparation of a wide-range of polymers by a wide variety of different techniques, and describes how this synthetic methodology can be applied to the development of new materials. It includes details of well-established techniques, e.g. chain-growth or step-growth processes together with more up-to-date examples using methods such as atom-transfer radical polymerization. Less well-known procedures are also included, e.g. electrochemical synthesis of conducting polymers and the preparation of liquid crystalline elastomers with highly ordered structures. Other topics covered include general polymerization methodology, controlled/"living" polymerization methods, the formation of cyclic oligomers during step-growth polymerization, the synthesis of conducting polymers based on heterocyclic compounds, dendrimers, the preparation of imprinted polymers and liquid crystalline polymers. The main bulk of the text is preceded by an introductory chapter detailing some of the techniques available to the scientist for the characterization of polymers, both in terms of their chemical composition and in terms of their properties as materials. The book is intended not only for the specialist in polymer chemistry, but also for the organic chemist with little experience who requires a practical introduction to the field.

A p-Type PbS Quantum Dot Ink with Improved Stability for Solution Processable Optoelectronics

2021 ◽  
Author(s):  
Fiaz Ahmed ◽  
John Hardin Dunlap ◽  
Perry J. Pellechia ◽  
Andrew Greytak
Keyword(s):  
Quantum Dot ◽  
Ligand Exchange ◽  
Chemical Characterization ◽  
Single Step ◽  
Improved Stability ◽  
Solution Processable ◽  
P Type ◽  
Pbs Quantum Dot

A highly stable p-type PbS-QDs ink is prepared using a single-step biphasic ligand exchange route, overcoming instability encountered in previous reports. Chemical characterization of the ink reveals 3-mercaptopriopionic acid (MPA)...

scholarly journals Synthesis and Polymerization Kinetics of Rigid Tricyanate Ester

Polymers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1686
Author(s):  
Andrey Galukhin ◽  
Roman Nosov ◽  
Ilya Nikolaev ◽  
Elena Melnikova ◽  
Daut Islamov ◽  
...  
Keyword(s):  
Kinetic Analysis ◽  
Single Step ◽  
Polymerization Kinetics ◽  
Scanning Calorimetry ◽  
Modulated Differential Scanning Calorimetry ◽  
Diffusion Controlled ◽  
Polymerization Product ◽  
The One ◽  
Kinetics Of

A new rigid tricyanate ester consisting of seven conjugated aromatic units is synthesized, and its structure is confirmed by X-ray analysis. This ester undergoes thermally stimulated polymerization in a liquid state. Conventional and temperature-modulated differential scanning calorimetry techniques are employed to study the polymerization kinetics. A transition of polymerization from a kinetic- to a diffusion-controlled regime is detected. Kinetic analysis is performed by combining isoconversional and model-based computations. It demonstrates that polymerization in the kinetically controlled regime of the present monomer can be described as a quasi-single-step, auto-catalytic, process. The diffusion contribution is parameterized by the Fournier model. Kinetic analysis is complemented by characterization of thermal properties of the corresponding polymerization product by means of thermogravimetric and thermomechanical analyses. Overall, the obtained experimental results are consistent with our hypothesis about the relation between the rigidity and functionality of the cyanate ester monomer, on the one hand, and its reactivity and glass transition temperature of the corresponding polymer, on the other hand.

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