An improved synthetic growth medium for Halobacterium cutirubrum

1976 ◽  
Vol 22 (3) ◽  
pp. 440-442 ◽  
Author(s):  
V. L. Grey ◽  
P. S. Fitt
Keyword(s):  
Growth Medium ◽  
Synthetic Medium ◽  
Complex Media ◽  
Synthetic Growth Medium ◽  
Synthetic Growth

A synthetic medium is described in which Halobacterium cutirubrum grows as well as in complex media.

scholarly journals Selective synthetic growth medium “Acinetobacter phenylalanine agar” for isolation and identification of Acinetobacter calcoaceticus — Acinetobacter baumannii complex species

2020 ◽  
Vol 10 (3) ◽  
pp. 591-596
Author(s):  
E. P. Sivolodskii ◽  
G. V. Gorelova ◽  
S. P. Bogoslovskaya ◽  
E. V. Zueva
Keyword(s):  
Growth Medium ◽  
Acinetobacter Calcoaceticus ◽  
Selective Growth ◽  
Isolation And Identification ◽  
Complex Species ◽  
Synthetic Growth Medium ◽  
Acinetobacter Baumannii Complex ◽  
Acinetobacter Spp ◽  
Routine Assay ◽  
Synthetic Growth

The aim of the study was to carry out clinical and microbiological testing of selective growth medium Acinetobacter phenylalanine agar to isolate and identify bacterial species belonging to the Acinetobacter calcoaceticus — Acinetobacter baumannii complex (ACB complex). For this, 400 samples of clinical material (wound discharge, blood, urine, bronchoalveolar lavage) were examined in 2018 in the Clinical and Bacteriological Laboratory of the Military Medical Academy by using routine assays (seeding on blood agar growth medium, pure culture isolation and identification by using biochemical assays as well as VITEK 2 microbial identification system, bioMerieux) and plating together with growth medium Acinetobacter phenylalanine agar selective to Acinetobacter spp. owing to L-phenylalanine as a sole nitrogen and carbon source additional selected with trimethoprim. ACB complex Acinetobacters were identified 18–24 hours later after incubation at 37°C by emergence of typical colonies on selective medium. Control tests for cytochrome oxidase as well as oxidative/fermentation (OF)-glucose test by using peroxide-hydrogen microvolume method (for 1 h) allowed to rapidly distinguish ACB complex Acinetobacters from other bacteria as well as from Acinetobacter spp. unable to glucose oxidation. Next, species identity for all isolated Acinetobacter strains was established by using matrix-activated laser desorption/ionization with time-of-flight mass spectrometry (MALDI-TOF MS). It was found that by using novel vs. routine assay Acinetobacter spps. were isolated in 26 (18 samples — in monoculture, 8 — in association with Pseudomonas aeruginosa or Klebsiella pneumoniae subsp. pneumoniae with tiny associate colonies) vs. 20 (7 — in monoculture, 13 — in association with Pseudomonas aeruginosa, Klebsiella, Escherichia, Citrobacter, Providencia, Staphylococcus, Enterococcus, C. albicans). Using MALDI-TOF MS method revealed that 25 out of 26 Acinetobacter strains isolated on selective growth medium belonged to the ACB complex (A. baumannii — 23, A. pittii — 2), whereas one strain (A. baylyi) did not belong to ACB complex. Hence, the diagnostic specificity of the Acinetobacter phenylalanine agar synthetic growth medium for isolation and identification of the A. calcoaceticus — A. baumannii complex species comprised 96.2%, with diagnostic sensitivity exceeding that one for routine assay by 25%. Use of selective growth medium accelerates research by allowing to isolate and identify ACB complex Acinetobacter spp. 18–24 h later after plating clinical material. Selectivity of growth medium was potentially stable, as its major trophic selection factor did not depend on acquired bacterial antibiotic resistance, which is also suitable as a synthetic growth medium for standardized studies.

Adenosine-induced bacteriostasis in Micrococcus sodonensis

1973 ◽  
Vol 19 (9) ◽  
pp. 1083-1092 ◽  
Author(s):  
Charles R. Shobe ◽  
J. N. Campbell
Keyword(s):  
Growth Inhibition ◽  
Growth Medium ◽  
Chromatographic Analysis ◽  
De Novo ◽  
Pyrimidine Biosynthesis ◽  
Bacteriostatic Effect ◽  
Purine Bases ◽  
De Novo Biosynthesis ◽  
Synthetic Growth Medium ◽  
Synthetic Growth

In a synthetic growth medium at a concentration of 1.0 mM, adenosine had a marked bacteriostatic effect on the growth of Micrococcus sodonensis. With the exceptions of hypoxanthine and inosine, which were slightly inhibitory, other purine bases and ribonucleosides did not inhibit the growth of the organism. When incubation of adenosine-supplemented cultures was prolonged, the organism was able to recover from the growth inhibition after a period of time which varied directly with the initial adenosine concentrations. Chromatographic analysis of the supernatants of those cultures revealed that exogenous adenosine concentrations declined steadily and the termination of the inhibited phase of growth correlated with the exhaustion of exogenous adenosine.It was observed that de novo biosynthesis of thiamine was inhibited in adenosine-supplemented cultures and that this inhibition was also relieved upon the exhaustion of exogenous adenosine. Bacteriostasis could be prevented by including either thiamine or its pyrimidine precursor (B1-pyrimidine) in adenosine-supplemented cultures and it was concluded that the observed growth inhibition reflected a depletion of the intracellular thiamine pool resulting from an adenosine-associated inhibition of B1-pyrimidine biosynthesis.

Repair of salt tolerance and recovery of lost D-alanine and magnesium following sublethal heating of Staphylococcus aureus are independent events

1981 ◽  
Vol 27 (6) ◽  
pp. 627-632 ◽  
Author(s):  
A. Hurst ◽  
A. Hughes
Keyword(s):  
Staphylococcus Aureus ◽  
Salt Tolerance ◽  
Phosphate Buffer ◽  
Synthetic Medium ◽  
Potassium Phosphate ◽  
Complex Media ◽  
Rich Medium ◽  
Potassium Phosphate Buffer ◽  
Heat Damage ◽  
Independent Events

Sublethal heating of Staphylococcus aureus S6 in potassium phosphate buffer caused loss of salt tolerance, D-alanine, and magnesium. During incubation in rich complex media all three of the damaged sites were repaired. Repair occurred more slowly but went to completion in a dilute synthetic medium (DSM), free of D-ala. DSM plus penicillin or D-cycloserine allowed repair of salt tolerance but recovery of normal levels of D-ala or Mg was prevented. When DSM-repaired cells were cultured into fresh rich medium they grew rapidly after a short lag. Cells which had acquired their salt tolerance in DSM plus cycloserine and were D-ala and Mg deficient grew slowly and had a lag of 3 h. We suggest that heat damage has two separate primary targets in S. aureus cells: the membrane, which is manifested by loss of salt tolerance, and a second site, possibly teichoic acids, manifested by loss of D-ala and Mg.

Variations du contenu lipidique occasionnées par la carence en pyridoxine du milieu nutritif de Ceratocystis ulmi

1984 ◽  
Vol 62 (1) ◽  
pp. 102-104
Author(s):  
Y. Dalpé
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Lipid Fraction ◽  
Fatty Acid Content ◽  
Neutral Lipid Fraction ◽  
Polar Lipid Fraction ◽  
Polyunsaturated Fatty Acid Content ◽  
Synthetic Growth Medium ◽  
Total Disappearance ◽  
Synthetic Growth

Pyridoxin deficiency of the synthetic growth medium results on four of the five strains of the studied Ceratocystis ulmi in an increase (6.9 to 18.8%) of the lipid content of the mycelium. This increase mainly affects the neutral lipid fraction. The analysis of fatty acids shows a significative decrease, both in the neutral and the polar lipid fraction, of the polyunsaturated fatty acid content, and in some cases the total disappearance of the linolenic acid.

Effects of radiation pasteurization on Salmonella. I. Parameters affecting survival and recovery from chicken

1970 ◽  
Vol 16 (6) ◽  
pp. 465-471 ◽  
Author(s):  
Joseph J. Previte ◽  
Y. Chang ◽  
H. M. El-Bisi
Keyword(s):  
Ionizing Radiation ◽  
Salmonella Typhimurium ◽  
Growth Medium ◽  
Yeast Extract ◽  
Brain Heart Infusion ◽  
Complex Media ◽  
Germ Free ◽  
Typhimurium Strain ◽  
Effects Of Radiation ◽  
Different Strains

Growth of Salmonella typhimurium, strain RIA, in minimal broth supplemented with methionine, produced a population of cells that was more sensitive to 60Co irradiation than were those grown in brain heart infusion. The resistance values (D) were 0.056 and 0.066 Mrad, respectively. D was not significantly different when the bacteria were irradiated at 4 °C on fresh or frozen-thawed germ-free chicken, or on conventional chicken sterilized by autoclaving, or by exposure to 2 Mrad of ionizing radiation. It was generally higher when the cells were irradiated in the original growth medium than on chicken. D of five different strains of S. typhimurium irradiated at 4 °C on autoclaved chicken varied from 0.052 to 0.068 Mrad. The greatest increase in resistance was produced by decreasing the temperature during irradiation. S. typhimurium, RIA, attained a D value of 0.293 Mrad at −80 °C. The effect of the recovery media on survival is equivocal. In some instances nutrient and minimal agar seemed to provide slightly better recovery of strains SR-11 and RIA. Enrichment of complex media with yeast extract did not increase recovery. The results indicate that of those parameters studied, the most significant effects on resistance were due to the temperature during irradiation.

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