Membrane mutations and production of enterotoxin B and alpha hemolysin in Staphylococcus aureus

1975 ◽  
Vol 21 (3) ◽  
pp. 275-280 ◽  
Author(s):  
Robert A. Altenbern
Keyword(s):  
Staphylococcus Aureus ◽  
Aureus Strain ◽  
Type B ◽  
Alpha Hemolysin ◽  
Osmotic Stability ◽  
Enterotoxin B

Staphylococcus aureus strain S-6, which produces enterotoxin type B (SEB), and strain 10-275, a high toxin-producing mutant derived from S-6, display pronounced differences in dye sensitivity, osmotic stability, and bacitracin sensitivity. Such characteristics are consistent with the concept that strain 10-275 is a membrane mutant of strain S-6. Some membrane mutants of S. aureus strain 14458 exhibit about two- to three-fold increases in SEB production whereas other membrane mutants show about twofold increases in α-hemolysin production. It is suggested that specific and independent membrane mutations control the secretory processes resulting in the extracellular elaboration of these exoproteins.

Enterotoxin B production by Staphylococcus aureus under controlled fatty acid nutrition induced by cerulenin

1977 ◽  
Vol 23 (9) ◽  
pp. 1145-1150 ◽  
Author(s):  
Robert A. Altenbern
Keyword(s):  
Fatty Acids ◽  
Staphylococcus Aureus ◽  
Fatty Acid ◽  
Membrane Fluidity ◽  
Unsaturated Fatty Acid ◽  
Unsaturated Fatty Acids ◽  
Basal Medium ◽  
Aureus Strain ◽  
Enterotoxin B ◽  
Fatty Acid Supplement

Cells of Staphylococcus aureus, strain S-6, can grow in the presence of 100 μg of cerulenin/ml if the basal medium is supplemented with certain saturated or unsaturated fatty acids. The production of enterotoxin B (SEB) is markedly influenced by both the ratio of saturated to unsaturated fatty acid and by the melting point of the unsaturated fatty acid supplement. The results presented suggest that a certain degree of membrane fluidity promotes maximum SEB production and that greater or lesser degrees of membrane fluidity prohibit substantial SEB formation but fail to affect final growth density.

Effect of Magnesium and Iron on the Growth of and Production of Extracellular Proteins by Staphylococcus aureus Strain S-6

1988 ◽  
Vol 51 (9) ◽  
pp. 731-733 ◽  
Author(s):  
ELISA YOKO HIROOKA ◽  
SONIA PRESA C. de SALZBERG ◽  
MERLIN S. BERGDOLL
Keyword(s):  
Staphylococcus Aureus ◽  
Dry Weight ◽  
Extracellular Proteins ◽  
Cellular Morphology ◽  
Dnase Activity ◽  
Aureus Strain ◽  
Gram Negative ◽  
Enterotoxin B ◽  
Mineral Ions

The effect of magnesium and iron on the growth, cellular morphology, deoxyribonuclease, coagulase, and enterotoxin B (SEB) production of Staphylococcus aureus strain S-6 in a pancreatic digest of casein (NAK) which had been treated with alumina to remove mineral ions was determined. Growth of S. aureus in the treated NAK medium (NAKSA) was minimal; the morphology of the cells was heterogenous with many large cells as well as some that were gram negative. The cells gradually reverted towards normal as the Mg2+ concentration was increased to 1.1 μg/ml. Cell dry weight increased from 0.36 ± 0.27 mg/ml to 1.16 ± 0.41 mg/ml, DNase activity increased from 7.6 units/mg dry weight to 77.0 units/mg dry weight, and SEB production increased from 12.2 to 54.3 μg/mg cell dry weight when the Mg2+ content was increased to 1.1 μg/ml. Increasing the Fe2+ content above the 0.4 μg/ml in the NAKSA medium containing 1.1 μg/Mg2+ resulted in decreases in dry weight and DNase activity, a slight increase in SEB production, and a relatively large increase in coagulase production.

scholarly journals Bovine abortion associated with Staphylococcus aureus infection - characterization of S. aureus strain isolated from fetal tissues

2020 ◽  
Vol 50 (6) ◽  
Author(s):  
Luan Cleber Henker ◽  
Marina Paula Lorenzett ◽  
Camila Imperico Riboldi ◽  
Franciele Maboni Siqueira ◽  
David Driemeier ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Fetal Loss ◽  
Multidrug Resistant ◽  
Aureus Strain ◽  
Alpha Hemolysin ◽  
Aureus Infection ◽  
Bronchiolar Epithelium ◽  
Toxic Shock Syndrome Toxin ◽  
Bovine Abortion

ABSTRACT: Staphylococcus aureus is a gram-positive bacterium, commonly found colonizing the skin and mucous membranes of humans and animals. This report describes a case of fetal loss associated with S. aureus infection in a cow. A six-month old, crossbred male bovine fetus from a beef farm was submitted for necropsy. At gross examination fibrinous pleuropneumonia was observed. Histologically, lesions were restricted to the lungs and consisted of marked multifocal to coalescing areas of inflammatory infiltrate of neutrophils, abundant fibrin exudation, necrosis of bronchiolar epithelium and numerous aggregates of coccoid bacteria. Lung and abomasal fluid bacterial culture yielded pure culture of S. aureus, which was characterized as a multidrug resistant strain. Molecular analysis indicated that the studied strain presented several genes of virulence factors including toxic shock syndrome toxin-1 (tst), staphylococcal enterotoxin type A (sea), Panton-Valentine leukocidin (pvl), alpha-hemolysin (hla) and delta-hemolysin (hld). This report documents an infrequent case of fetal loss in cattle due to infection with a highly virulent S. aureus strain.

Synthesis of enterotoxin B by Staphylococcus aureus strain S6 after recovery from heat injury

1973 ◽  
Vol 19 (12) ◽  
pp. 1463-1468 ◽  
Author(s):  
D. L. Collins-Thompson ◽  
A. Hurst ◽  
H. Kruse
Keyword(s):  
Heat Treatment ◽  
Staphylococcus Aureus ◽  
Salt Tolerance ◽  
Sodium Phosphate ◽  
Potassium Phosphate ◽  
Aureus Strain ◽  
Heat Injury ◽  
K Cells ◽  
Enterotoxin B ◽  
And Control

After sublethal heat treatment of Staphylococcus aureus S6 at 52C for 15 min in either 0.1 M sodium phosphate (Na cells) or 0.1 M potassium phosphate (K cells), more than 99% of the cells were unable to grow on a medium containing 7.5% NaCl. When placed in H and K medium the survivors recovered their salt tolerance and grew after a lag of 3 h (Na cells) or 5 h (K cells). In the absence of glucose, the total amount of enterotoxin B synthesized by the Na and K cells was similar to the control cells. Addition of 0.1% glucose to the medium increased the total amount of toxin formed by Na, K, and control cells.

scholarly journals Cloning and Expression of Alpha Hemolysin Toxin Gene of Staphylococcus aureus Against Human Cancer Tissue

2017 ◽  
Vol 5 (1) ◽  
pp. 22-29 ◽  
Author(s):  
Gamal M. Abdel-Fattah ◽  
Elsayed E. Hafez ◽  
Mysaa E. Zaki ◽  
Nada M. Darwesh
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Cloning And Expression ◽  
Human Lung Cancer ◽  
Aureus Strain ◽  
Toxin Gene ◽  
Cancer Tissue ◽  
Alpha Hemolysin

Recombinant technology has crucial impact in therapy development. In microbial environment, Pathogenic bacteria such as Staphylococcus aureus produces alpha hemolysin protein. This protein is used as anticancer protein. In our study, alpha hemolysin toxin (hia) gene was isolated from S. aureus isolate. The isolate of S. aureus was isolated from blood samples of patients at Microbiology laboratory of Chlidren Mansoura Unversity Hospital. The purified PCR of hia gene of S. aureus strain was subjected to sequencing and cloning. Recombinant alpha hemolysin toxin cloning was detected by PCR, and α- hemolysin protein was purified by Sphadex. The purified α-protein was used as anticancer against HepG-2 cells (human Hepatocellular carcinoma), HCT-116 cells (human colon carcinoma), MCF-7 cells (human breast cancer cell line) and A-549 cells (human Lung cancer cell line). Int. J. Appl. Sci. Biotechnol. Vol 5(1): 22-29

Synthesis of Enterotoxin by L-Forms of Staphylococcus aureus

1970 ◽  
Vol 1 (2) ◽  
pp. 169-173
Author(s):  
Joyce K. Czop ◽  
Merlin S. Bergdoll
Keyword(s):  
Staphylococcus Aureus ◽  
Type A ◽  
Prolonged Treatment ◽  
Type B ◽  
Enterotoxin B

After prolonged treatment with methicillin and penicillin, L-forms were prepared from 12 of 22 strains of Staphylococcus aureus . These L-forms were obtained from five of nine strains which produced enterotoxin A, from three of six strains that produced enterotoxin B, and from three of six strains that produced enterotoxin C. An L-form was also prepared from S. aureus strain AH, which produces both enterotoxins A and C. The synthesis of enterotoxin A by parent strains was least affected by the conditions required for L-form growth. Detectable amounts of enterotoxin A were produced by all of the revertants tested and by five of the six L-forms which were derived from type A-producing cells. Detectable amounts of enterotoxin B were produced by four of the five revertants but by none of the L-forms derived from type B-producing cells. Revertants and L-forms from cells which produced enterotoxin C did not produce enough enterotoxin C to be detectable on microslides.

Imperatorin inhibits the expression of alpha-hemolysin in Staphylococcus aureus strain BAA-1717 (USA300)

2016 ◽  
Vol 109 (7) ◽  
pp. 915-922 ◽  
Author(s):  
Ping Ouyang ◽  
Junjie Chen ◽  
Mao Sun ◽  
Zhongqiong Yin ◽  
Juchun Lin ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Aureus Strain ◽  
Alpha Hemolysin

Protein A of Staphylococcus aureus strain NCTC8325 interacted with heparin

2021 ◽  
Author(s):  
Hui Shi ◽  
Jiaqin Tang ◽  
Cuiying An ◽  
Lingkang Yang ◽  
Xianxuan Zhou
Keyword(s):  
Staphylococcus Aureus ◽  
Protein A ◽  
Aureus Strain
Sign in / Sign up

Export Citation Format

Share Document