Action of chlorpromazine on spore-forming Bacillus species

1974 ◽  
Vol 20 (12) ◽  
pp. 1689-1693 ◽  
Author(s):  
Michael Orlowski ◽  
Manuel Goldman
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Megaterium ◽  
Mechanism Of Action ◽  
Bacillus Species ◽  
Hexose Monophosphate ◽  
Inhibitory Effects ◽  
Hexose Monophosphate Shunt ◽  
Bacterial Systems

The hypothesis that chlorpromazine inhibition of bacterial endospore germination acts at the level of hexose monophosphate shunt (HMP) dehydrogenases was tested and substantiated for Bacillus cereus T and Bacillus megaterium. However, this cannot be an exclusive mechanism of action in bacterial systems because inhibitory effects on growth and respiration are caused by the drug even when these HMP enzymes are not present in the organisms.

Isolation, identification and in silico study of native cellulase producing bacteria

2019 ◽  
Vol 17 ◽  
Author(s):  
Farzane Kargar ◽  
Mojtaba Mortazavi ◽  
Mahmood Maleki ◽  
Masoud Torkzadeh Mahani ◽  
Younes Ghasemi ◽  
...  
Keyword(s):  
Growth Rate ◽  
Bacillus Cereus ◽  
16S Rdna ◽  
Enzyme Production ◽  
Rational Design ◽  
Cellulase Production ◽  
Bacillus Species ◽  
Bacillus Sp ◽  
Chain Polymer ◽  
Bacillus Toyonensis

Aims: The purpose of this study was to screen the bacteria producing cellulase enzymes and their bioinformatics studies. Background: Cellulose is a long-chain polymer of glucose that hydrolyzes by cellulases to glucose molecules. In order to design the new biotechnological applications, some strategies have been used as increasing the efficiency of enzyme production, generating cost-effective enzymes, producing stable enzymes and identification of new strains. Objective: On the other hand, some bacteria special features have made them suitable candidates for the identification of the new source of enzymes. In this regard, some native strains of bacteria were screened. Method: These bacteria were grown on a culture containing the liquid M9 media containing CMC to ensure the synthesis of cellulase. The formation of a clear area in the culture medium indicated decomposition of cellulose. In the following, the DNA of these bacteria were extracted and their 16S rDNA genes were amplified. Result: The results show that nine samples were able to synthesize cellulase. In following, these strains were identified using 16S rDNA. The results show that these screened bacteria belonged to the Bacillus sp., Alcaligenes sp., Alcaligenes sp., and Enterobacter sp.conclusionThe enzyme activity analysis shows that the Bacillus toyonensis, Bacillus sp. strain XA15-411 Bacillus cereus have produced the maximum yield of cellulases. However, these amounts of enzyme production in these samples are not proportional to their growth rate. As the bacterial growth chart within 4 consecutive days shows that the Alcaligenes sp. Bacillus cereus, Bacillus toyonensis, Bacillus sp. strain XA15-411 have a maximum growth rate. The study of the phylogenetic tree also shows that Bacillus species are more abundant in the production of cellulase enzyme. These bioinformatics analyses show that the Bacillus species have different evolutionary relationships and evolved in different evolutionary time. Other: However, for maximum cellulase production by this bacteria, some information as optimum temperature, optimum pH, carbon and nitrogen sources are needed for the ideal formulation of media composition. The cellulase production is closely controlled in microorganisms and the cellulase yields appear to depend on a variety of factors. However, the further studies are needed for cloning, purification and application of these new microbial cellulases in the different commercial fields as in food, detergent, and pharmaceutical, paper, textile industries and also various chemical industries. However, these novel enzymes can be further engineered through rational design or using random mutagenesis techniques.

Cytoplasmic pH regulation in phorbol ester-activated human neutrophils

1986 ◽  
Vol 251 (1) ◽  
pp. C55-C65 ◽  
Author(s):  
S. Grinstein ◽  
W. Furuya
Keyword(s):  
Metabolic Pathways ◽  
Phorbol Esters ◽  
Ph Regulation ◽  
Human Neutrophils ◽  
Hexose Monophosphate ◽  
Cytoplasmic Ph ◽  
Extracellular Acidification ◽  
Hexose Monophosphate Shunt ◽  
Activated Neutrophils ◽  
Cytoplasmic Acidification

Activation of neutrophils by 12-O-tetradecanoylphorbol-13-acetate (TPA) is accompanied by an initial cytoplasmic acidification, followed by an alkalinizing phase due to Na+-H+ countertransport. The source of the acidification, which is fully expressed by activation with TPA in Na+-free or amiloride-containing media, was investigated. The acidification phase was detected also in degranulated and enucleated cytoplasts, ruling out a major contribution by the nucleus or secretory vesicles. Cytoplasmic acidification was found to be associated with an extracellular acidification, suggesting metabolic generation of H+. Two principal metabolic pathways are stimulated in activated neutrophils: the reduction of O2 by NADPH-oxidase and the hexose monophosphate shunt. A good correlation was found between the activity of these pathways and the changes in cytoplasmic pH. Inhibition of superoxide synthesis prevented the TPA-induced cytoplasmic acidification. Moreover, activation of the hexose monophosphate shunt with permeable NADPH-oxidizing agents (in the absence of TPA) also produced a cytoplasmic acidification. Cytoplasmic acidification was also elicited by exogenous diacylglycerol and by other beta-phorbol diesters, which are activators of the kinase, but not by unesterified phorbol or by alpha-phorbol diesters, which are biologically inactive. The results suggest that the cytoplasmic acidification induced by phorbol esters in neutrophils reflects accumulation of H+ liberated during the metabolic burst that follows activation.

scholarly journals Canine pyometra associated with Bacillus species: a case report

2018 ◽  
Vol 63 (No. 3) ◽  
pp. 143-149 ◽  
Author(s):  
MK Kim ◽  
HY Yoon ◽  
MH Lee ◽  
JH Kim
Keyword(s):  
Mammary Gland ◽  
Case Report ◽  
Bacillus Megaterium ◽  
Fatal Outcome ◽  
Bacillus Species ◽  
Bacterial Isolation ◽  
Sonographic Examination ◽  
First Time ◽  
Gland Tumour ◽  
Unilateral Mastectomy

An 11-year-old, female Maltese was presented for evaluation of a mammary gland tumour. An abdominal sonographic examination showed an echogenic, fluid-filled, dilated uterus; pyometra was also diagnosed. Ovariohysterectomy and unilateral mastectomy was performed and the dog recovered uneventfully. However, 11 h later, the dog’s condition suddenly deteriorated and it died on the day after the surgery. Bacillus circulans and Bacillus megaterium were isolated from its uterine content; these species had not been reported previously in canine pyometra. The two species were resistant to various antibiotics, including cefazolin used during the treatment. We describe for the first time the presentation, diagnosis, bacterial isolation and fatal outcome of B. circulans and B. megaterium infection in a Maltese dog with pyometra.

scholarly journals Macrophage variants in oxygen metabolism.

1980 ◽  
Vol 152 (4) ◽  
pp. 808-822 ◽  
Author(s):  
G Damiani ◽  
C Kiyotaki ◽  
W Soeller ◽  
M Sasada ◽  
J Peisach ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cytochrome C ◽  
Superoxide Anion ◽  
Oxygen Metabolism ◽  
Hexose Monophosphate ◽  
Phagocytic Cells ◽  
Form Complex ◽  
Hexose Monophosphate Shunt ◽  
Cytochrome C Reduction ◽  
Generate Superoxide Anion

Whereas phagocytic cells from normal individuals have the capacity to kill ingested bacteria and parasites, those from patients with several uncommon genetic deficiency diseases are known to be defective in bactericidal activity. Studies on neutrophils of these patients have revealed fundamental defects in their ability to reduce molecular oxygen and metabolize it to superoxide anion, hydrogen peroxide, and oxygen radicals. In the present experiments, we describe a clone of a continuous murine macrophage-like cell line, J774.16, that, upon appropriate stimulation, activates the hexose monophosphate shunt, and produces superoxide anion and hydrogen peroxide. With nitroblue tetrazolium to select against cells capable of being stimulated by phorbol myristate acetate to reduce the dye to polymer--formazan--which is toxic fot cells, we have selected for variants that are defective in oxygen metabolism. Four of these subclones have been characterized and found to be lacking in the ability (a) to generate superoxide anion, as measured by cytochrome c reduction; (b) to produce hydrogen peroxide, as measured by the ability to form complex I with cytochrome c peroxidase; and (c) to be stimulated to oxidize glucose via the hexose monophosphate shunt. These variants appear to represent a useful model for studying the molecular basis for macrophage cytocidal activity.

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