Serotyping of Proteus rettgeri on the basis of O antigens

1974 ◽  
Vol 20 (5) ◽  
pp. 777-789 ◽  
Author(s):  
John L. Penner ◽  
Norman A. Hinton ◽  
Joan Hennessy
Keyword(s):  
Common Antigen ◽  
Definition Of ◽  
O Antigens ◽  
Type Strains

A serotyping system for Proteus rettgeri on the basis of 72 O antigens has been developed in a study of 324 strains obtained from widely distributed sources. In the investigation of the interrelations among the 72 O-type strains by cross-titration of each of the 72 anti-O sera with antigen of each of the O-type strains, both reciprocal and nonreciprocal (unilateral) reactions were observed. The presence of the Kunin common enterobacterial antigen (CA) was noted in each strain of P. rettgeri examined, and the unilateral reactions of two antisera were shown to be due to antibody present against CA. This indicated that CA was in the immunogenic state in two strains of this species. A second common antigen resembling CA in some respects but differing in specificity and in its agglutinability was shown to be the cause of unilateral reactions in a third antiserum. In other cases, unilateral reactive antibodies appeared to be directed against antigens that remained undefined. Each reciprocal reaction was investigated to determine the nature of the interrelations between interacting strains, and the definition of these led to the development of procedures for the production of absorbed anti-O sera with greater specificity. The serotyping scheme has increased the number of strains that may be typed through their O antigens, and its application in epidemiological investigations of infections due to P. rettgeri is indicated.

scholarly journals Pseudovibrio japonicus sp. nov., isolated from coastal seawater in Japan

2007 ◽  
Vol 57 (9) ◽  
pp. 1952-1955 ◽  
Author(s):  
Shoichi Hosoya ◽  
Akira Yokota
Keyword(s):  
Dna Hybridization ◽  
Type Strain ◽  
Novel Species ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Biochemical Characteristics ◽  
Boso Peninsula ◽  
Coastal Seawater ◽  
Definition Of ◽  
Type Strains

A Gram-negative, motile, rod-shaped bacterium (WSF2T) was isolated from coastal seawater of the Boso Peninsula in Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain WSF2T represented a separate lineage within the genus Pseudovibrio. The DNA G+C content of strain WSF2T was 51.7 mol%. DNA–DNA hybridization values between strain WSF2T and the type strains of Pseudovibrio species were significantly lower than those accepted as the phylogenetic definition of a species. Furthermore, some biochemical characteristics indicated that strain WSF2T differed from other Pseudovibrio species. Based on these characteristics, it is proposed that the isolate represents a novel species, Pseudovibrio japonicus sp. nov. The type strain is WSF2T (=IAM 15442T=NCIMB 14279T=KCTC 12861T).

scholarly journals Flammeovirga kamogawensis sp. nov., isolated from coastal seawater in Japan

2007 ◽  
Vol 57 (6) ◽  
pp. 1327-1330 ◽  
Author(s):  
Shoichi Hosoya ◽  
Akira Yokota
Keyword(s):  
Dna Hybridization ◽  
Novel Species ◽  
Rrna Gene ◽  
The Novel ◽  
Threshold Values ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Coastal Seawater ◽  
Definition Of ◽  
Type Strains

Two strains of gliding, agarolytic bacteria, strains YS10T and YML5, were isolated from coastal seawater off Kamogawa, Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel isolates represent a separate lineage within the genus Flammeovirga. DNA–DNA hybridization values between these isolates and the type strains of species of the genus Flammeovirga were significantly lower than those accepted as threshold values for the phylogenetic definition of a species. Furthermore, some of the phenotypic characteristics indicate that the isolates differ from other Flammeovirga species. Based on these differences, it is suggested that the isolates represent a novel species, for which the name Flammeovirga kamogawensis sp. nov. is proposed. The type strain is YS10T (=IAM 15451T=NCIMB 14281T).

scholarly journals Preparation of typing antisera specific for O antigens of Pseudomonas aeruginosa

1976 ◽  
Vol 4 (2) ◽  
pp. 124-128
Author(s):  
N H Duncan ◽  
N A Hinton ◽  
J L Penner ◽  
I B Duncan
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cross Reactivity ◽  
Cell Suspensions ◽  
Clinical Isolates ◽  
Cross Reactions ◽  
High Incidence ◽  
The Cross ◽  
O Antigens ◽  
Type Strains

Results of serotyping 966 clinical isolates of Pseudomonas aeruginosa showed that 72% agglutinated specifically in one or another of the 16 typing antisera, but 28% agglutinated in two or more and often in as many as 10 antisera; this polyagglutinability correlated with a high incidence of cross-reactivity among the antisera. Absorption of each typing antiserum with either cell suspensions of five O-type strains or with a suspension of a particular polyagglutinable strain (SMC 247) abolished cross-reactivity in the typing antisera without significantly reducing titers against the homologous strains. All but four of the polyagglutinable strains agglutinated specifically in one or another absorbed antisera. The cross-reactions of unabsorbed antisera were interpreted to have been caused by antibodies directed not against specific O antigens but against thermostable specificities that remain undefined.

scholarly journals Antigenic scheme for Citrobacter koseri (syn. C. diversus, Levinea malonatica); three new antigens recognized in strains from Israel

1981 ◽  
Vol 86 (1) ◽  
pp. 111-115 ◽  
Author(s):  
R. J. Gross ◽  
B. Rowe ◽  
I. Sechter ◽  
D. Cahan ◽  
G. Altman
Keyword(s):  
O Antigens ◽  
Type Strains

SUMMARYAn antigenic scheme for Citrobacter koseri was described previously and consisted of 14 ‘O’ antigens. Three additional antigens are now added to the scheme and type strains for these antigens are designated. Their origins and their biochemical and serological reactions are described.

scholarly journals Aquimarina macrocephali sp. nov., isolated from sediment adjacent to sperm whale carcasses

2010 ◽  
Vol 60 (10) ◽  
pp. 2298-2302 ◽  
Author(s):  
Masayuki Miyazaki ◽  
Yuriko Nagano ◽  
Yoshihiro Fujiwara ◽  
Yuji Hatada ◽  
Yuichi Nogi
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Sperm Whale ◽  
Gliding Motility ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Dna Relatedness ◽  
Definition Of ◽  
Type Strains

A Gram-negative, rod-shaped, non-spore-forming, strictly aerobic strain with gliding motility, designated JAMB N27T, was isolated from sediment adjacent to sperm whale carcasses off Kagoshima, Japan, at a depth of 219 m. Strain JAMB N27T contained MK-6 as the major isoprenoid quinone and iso-C15 : 0, iso-C15 : 1, C16 : 1 and iso-C17 : 1 as the predominant fatty acids. Casein, chitin, gelatin and starch were degraded. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JAMB N27T represented a separate lineage within the genus Aquimarina. The DNA G+C content of strain JAMB N27T was 33.1 mol%. DNA–DNA relatedness values between strain JAMB N27T and type strains of species of the genus Aquimarina were significantly lower than the cut-off value accepted for the definition of a novel species. Therefore, strain JAMB N27T represents a novel species, for which the name Aquimarina macrocephali sp. nov. is proposed. The type strain is JAMB N27T (=JCM 15542T=NCIMB 14508T).

scholarly journals A Novel Gene Required for Rhamnose-Glucose Polysaccharide Synthesis in Streptococcus mutans

1999 ◽  
Vol 181 (20) ◽  
pp. 6556-6559 ◽  
Author(s):  
Yoshihisa Yamashita ◽  
Yukie Shibata ◽  
Yoshio Nakano ◽  
Hiromasa Tsuda ◽  
Nobuo Kido ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Streptococcus Mutans ◽  
Common Antigen ◽  
E Coli ◽  
Novel Gene ◽  
Enterobacterial Common Antigen ◽  
Polysaccharide Synthesis ◽  
O Antigens

ABSTRACT Gene rgpG is required for biosynthesis of rhamnose-glucose polysaccharide (RGP) in Streptococcus mutans. Its deduced amino acid sequence had similarity to WecA, which initiates syntheses of enterobacterial common antigen and some O antigens in Escherichia coli. Gene rgpGcomplemented a wecA mutation of E. coli, suggesting that rgpG may function similarly in RGP synthesis.

scholarly journals Genome Analysis of Endobacterium cerealis, a Novel Genus and Species Isolated from Zea mays Roots in North Spain

2020 ◽  
Vol 8 (6) ◽  
pp. 939
Author(s):  
Esther Menéndez ◽  
Jose David Flores-Félix ◽  
Martha Helena Ramírez-Bahena ◽  
Jose M. Igual ◽  
Paula García-Fraile ◽  
...  
Keyword(s):  
Zea Mays ◽  
Phylogenetic Analyses ◽  
Hydrolytic Enzymes ◽  
Plant Colonization ◽  
Rrna Gene ◽  
Novel Genus ◽  
Phenotypic Differences ◽  
Novel Genus And Species ◽  
Definition Of ◽  
Type Strains

In the present work, we analyse the genomic and phenotypic characteristics of a strain named RZME27T isolated from roots of a Zea mays plant grown in Spain. The phylogenetic analyses of 16S rRNA gene and whole genome sequences showed that the strain RZME27T clustered with the type strains of Neorhizobium galegae and Pseudorhizobium pelagicum from the family Rhizobiaceae. This family encompasses several genera establishing symbiosis with legumes, but the genes involved in nodulation and nitrogen fixation are absent in its genome. Nevertheless, genes related to plant colonization, such as those involved in motility, chemotaxis, quorum sensing, exopolysaccharide biosynthesis and hydrolytic enzymes production were found. The comparative pangenomic analyses showed that 78 protein clusters present in the strain RZME27T were not found in the type strains of its closest relatives N. galegae and P. pelagicum. The calculated average nucleotide identity (ANI) values between the strain RZME27T and the type strains of N. galegae and P. pelagicum were 75.61% and 75.1%, respectively, similar or lower than those found for other genera from family Rhizobiaceae. Several phenotypic differences were also found, highlighting the absence of the fatty acid C19:0 cyclo ω8c and propionate assimilation. These results support the definition of a novel genus and species named Endobacterium cerealis gen. nov. sp. nov. whose type strain is RZME27T.

scholarly journals Phylogenomic Analyses of the Genus Pseudomonas Lead to the Rearrangement of Several Species and the Definition of New Genera

Biology ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 782
Author(s):  
Zaki Saati-Santamaría ◽  
Ezequiel Peral-Aranega ◽  
Encarna Velázquez ◽  
Raúl Rivas ◽  
Paula García-Fraile
Keyword(s):  
New Species ◽  
New Genera ◽  
Taxonomic Organization ◽  
Bacterial Systematics ◽  
The Creation ◽  
Phylogenomic Analyses ◽  
Definition Of ◽  
Type Strains

Pseudomonas is a large and diverse genus broadly distributed in nature. Its species play relevant roles in the biology of earth and living beings. Because of its ubiquity, the number of new species is continuously increasing although its taxonomic organization remains quite difficult to unravel. Nowadays the use of genomics is routinely employed for the analysis of bacterial systematics. In this work, we aimed to investigate the classification of species of the genus Pseudomonas on the basis of the analyses of the type strains whose genomes are currently available. Based on these analyses, we propose the creation of three new genera (Denitrificimonas gen nov. comb. nov., Neopseudomonas gen nov. comb. nov. and Parapseudomonas gen nov. comb. nov) to encompass several species currently included within the genus Pseudomonas and the reclassification of several species of this genus in already described taxa.

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