Fermentation patterns of some lactose-negative enteric bacteria
Organic acids produced during fermentative growth of many lactose-negative members of Enterobacteriaceae have been determined quantitatively by thin-layer chromatography. Nearly all organisms examined elaborated succinic acid and many also produced fumaric and (or) pyruvic acid in varying ratios. Some differentiation of the genera could be achieved by calculation of the ratio of succinic/fumaric acid or succinic/pyruvic acid. Anaerogenic Salmonella strains and all Shigella species exhibited a succinic to fumaric acid ratio of less than 1.0. Aerogenic Salmonella strains yielded a succinic to fumaric acid ratio of 2.3 to 4.3. Proteus vulgaris and Proteus mirabilis were the only species examined that produced pyruvic acid in addition to succinic acid and fumaric acid. Strains of Edwardsiella tarda showed a broad range of succinic to fumaric acid ratios from 2.4 to 8.5. The single strain of Providencia alcalifaciens that was examined yielded only succinic acid and could be readily differentiated from other organisms examined.