Thermal stability of ribosomes from a thermophilic and a mesophilic fungus

1973 ◽  
Vol 19 (6) ◽  
pp. 761-763 ◽  
Author(s):  
H. M. Miller ◽  
M. G. Shepherd
Keyword(s):  
Thermal Stability ◽  
Ion Concentration ◽  
Magnesium Ion ◽  
Penicillium Notatum ◽  
Ribosomal Subunits ◽  
Ion Concentrations ◽  
Thermal Stability Of

Ribosomes and ribosomal subunits from the thermophile Penicillium duponti were found to be more thermostable than the corresponding particles from the mesophile Penicillium notatum. The thermostability of the ribosomes from both organisms was dependent on magnesium ion concentration. The dissociation of the 80-S ribosomes into 60-S and 40-S subunits occurred at higher magnesium ion concentrations for the mesophile than the thermophile.

THE EFFECT OF ION CONCENTRATION AND pH ON THE THERMAL STABILITY OF A CANINE ADENOVIRUS

1967 ◽  
Vol 13 (9) ◽  
pp. 1139-1149 ◽  
Author(s):  
T. Yamamoto
Keyword(s):  
Thermal Stability ◽  
Salt Solution ◽  
Ion Concentration ◽  
Ph Adjustment ◽  
Ion Concentrations ◽  
Stabilizing Effect ◽  
High Concentrations ◽  
Ph Range ◽  
Thermal Stability Of

The inactivation of a canine adenovirus hemagglutinin and infectivity at 56 °C at varying times up to 1 h has been studied in the presence of various ions and concentrations of ions.Under conditions of uncontrolled pH 1–2 M NaCl and MgCl2 both stabilized the virus hemagglutinin and infectivity. Lower concentrations of these salts as well as 0.04 M phosphate, Hank's balanced salt solution, and minimal Eagle's medium had little stabilizing effect. Molar concentrations of phosphate and Tris butter were also found to stabilize the virus hemagglutinin.The pH adjustment of the suspending salt solution was found to be important since high concentrations of salts such as MgCl2 are quite acidic. Both 1 M MgCl2 and 1 M NaCl stabilized the virus in the pH range of 4–6, but inactivation was rapid at pH 3 and less rapid at pH 7. Ion concentrations lower than 1 M markedly reduced the hemagglutinin stabilization at optimun pH.

Magnesium deficiency in embryos of Xenopus laevis

Development ◽  
1977 ◽  
Vol 39 (1) ◽  
pp. 97-113
Author(s):  
John C. Miller ◽  
Richard Landesman
Keyword(s):  
Xenopus Laevis ◽  
Normal Development ◽  
Magnesium Deficiency ◽  
Deficiency Syndrome ◽  
Ion Concentration ◽  
Magnesium Ion ◽  
Magnesium Ions ◽  
Ion Content ◽  
Ion Concentrations ◽  
Rearing Temperature

Embryos of Xenopus laevis reared in media with various low (≤ 10-5 M) magnesium ion concentrations will exhibit differing degrees of a potentially lethal magnesium starvation syndrome depending on the ion concentration and rearing temperature. The higher the rearing temperature or the lower the magnesium ion content of the medium the more severely the syndrome will be expressed. (Normal development can be expected at temperatures 13–30 °C and magnesium ion concentrations > 10−5 M to 10−2 M.) The magnesium deficiency syndrome in Xenopus embryos is described in detail and compared with the normal and anucleolate conditions. The deficiency condition becomes manifest after hatching as retarded growth and differentiation with progressive paralysis and edema. At the same time alterations are observed in the pattern of soluble proteins. The use of magnesium ions as a probe for investigating developing systems is discussed.

scholarly journals The Effect of Divalent Metal Ions on the Temperature Stability of Poly(I:C) Duplex

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Evgeniya Usenko ◽  
Vladimir Valeev ◽  
Alexander Glamazda ◽  
Victor Karachevtsev
Keyword(s):  
Thermal Stability ◽  
Metal Ions ◽  
Concentration Dependence ◽  
Temperature Stability ◽  
Ion Concentration ◽  
Poly I:C ◽  
Divalent Metal Ions ◽  
Buffer Solution ◽  
Solution Ph ◽  
Thermal Stability Of

The effect of divalent ions on the thermal stability of the double-stranded polynucleotide polyinosinic:polycytidylic acid (poly(I:C)) in a buffer solution (pH 7) was studied by UV absorption spectroscopy and thermal denaturation by measuring the absorbance at 260 nm. The concentration dependence of the polynucleotide melting temperature (Tm) in the presence of metal ions (2.5 × 10−6–10−2 M) was obtained. It was found that the duplex thermal stability grew gradually upon an increase of Ca2+ and Mn2+ concentration. The thermal stability of the duplex in the presence of Cd2+, Ni2+, and Zn2+ rises up to an ion concentration of about 10−4 M and further falls. In contrast to these ions, the addition of Cu2+ to the solution leads to a sharp decline in Тm of the duplex at a concentration above 10−5 M. The observed difference in the influence of metal ions on the poly(I:C) thermal stability is explained by the different affinity of these ions to both the phosphate groups and the nitrogenous bases of the polynucleotides. Based on the analysis of the Tm concentration dependence, the dominant binding of each ion to the specific polynucleotide components was proposed.

The Ordered Phase Formation in Ti-Al-Ga Alloys

1970 ◽  
Vol 28 ◽  
pp. 440-441
Author(s):  
Shiro Fujishiro ◽  
Harold L. Gegel
Keyword(s):  
Thermal Stability ◽  
High Temperature ◽  
Titanium Alloys ◽  
Growth Kinetics ◽  
Stoichiometric Composition ◽  
Good Potential ◽  
Alpha Titanium ◽  
Cold Rolled ◽  
Kinetics Of ◽  
Thermal Stability Of

Ordered-alpha titanium alloys having a DO19 type structure have good potential for high temperature (600°C) applications, due to the thermal stability of the ordered phase and the inherent resistance to recrystallization of these alloys. Five different Ti-Al-Ga alloys consisting of equal atomic percents of aluminum and gallium solute additions up to the stoichiometric composition, Ti3(Al, Ga), were used to study the growth kinetics of the ordered phase and the nature of its interface.The alloys were homogenized in the beta region in a vacuum of about 5×10-7 torr, furnace cooled; reheated in air to 50°C below the alpha transus for hot working. The alloys were subsequently acid cleaned, annealed in vacuo, and cold rolled to about. 050 inch prior to additional homogenization

Microstructure studies of tungsten silicide schottky contacts on Gaas

1987 ◽  
Vol 45 ◽  
pp. 328-329
Author(s):  
Yih-Cheng Shih ◽  
E. L. Wilkie
Keyword(s):  
Thermal Stability ◽  
Field Effect Transistors ◽  
Schottky Contact ◽  
Schottky Contacts ◽  
Excellent Thermal Stability ◽  
Barrier Heights ◽  
Gaas Substrates ◽  
Film Adhesion ◽  
Threshold Voltages ◽  
Thermal Stability Of

Tungsten silicides (WSix) have been successfully used as the gate materials in self-aligned GaAs metal-semiconductor-field- effect transistors (MESFET). Thermal stability of the WSix/GaAs Schottky contact is of major concern since the n+ implanted source/drain regions must be annealed at high temperatures (∼ 800°C). WSi0.6 was considered the best composition to achieve good device performance due to its low stress and excellent thermal stability of the WSix/GaAs interface. The film adhesion and the uniformity in barrier heights and ideality factors of the WSi0.6 films have been improved by depositing a thin layer of pure W as the first layer on GaAs prior to WSi0.6 deposition. Recently WSi0.1 has been used successfully as the gate material in 1x10 μm GaAs FET's on the GaAs substrates which were sputter-cleaned prior to deposition. These GaAs FET's exhibited uniform threshold voltages across a 51 mm wafer with good film adhesion after annealing at 800°C for 10 min.

Thermal stability of Al-Cu-Fe icosahedral alloys

1991 ◽  
Vol 1 (12) ◽  
pp. 1823-1836 ◽  
Author(s):  
M. Bessière ◽  
A. Quivy ◽  
S. Lefebvre ◽  
J. Devaud-Rzepski ◽  
Y. Calvayrac
Keyword(s):  
Thermal Stability ◽  
Thermal Stability Of

Practical criteria for the thermal stability of a unidimensional superconductor

1994 ◽  
Vol 4 (4) ◽  
pp. 653-657
Author(s):  
B. Bonzi ◽  
M. El Khomssi ◽  
H. Lanchon-Ducauquis
Keyword(s):  
Thermal Stability ◽  
Thermal Stability Of

Time and thermal stability of magnetic properties of amorphous Fe80TM3B17 alloys

1998 ◽  
Vol 08 (PR2) ◽  
pp. Pr2-63-Pr2-66 ◽  
Author(s):  
R. Varga ◽  
P. Vojtaník ◽  
A. Lovas
Keyword(s):  
Thermal Stability ◽  
Magnetic Properties ◽  
Thermal Stability Of
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