Permeability increases in cells of Salmonella enteritidis induced by ethylenediaminetetraacetate and temperature treatments

1972 ◽  
Vol 18 (12) ◽  
pp. 1803-1807 ◽  
Author(s):  
J. R. Chipley ◽  
H. M. Edwards Jr
Keyword(s):  
Ribonucleic Acid ◽  
Cell Walls ◽  
Untreated Control ◽  
Salmonella Enteritidis ◽  
Deoxyribonucleic Acid ◽  
Actinomycin D ◽  
Temperature Dependent ◽  
Lethal Effects ◽  
Treated Cell ◽  
Uptake And Release

Treatment of cells of Salmonella enteritidis with ethylenediaminetetraacetate (EDTA) resulted in losses of less than 4% of the total cellular deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and protein. No lethal effects could be observed when treated cells were plated and counted. The uptake of 14C-actinomycin-D, 22Na+, and 65Zn2+ into EDTA-treated cells and the release of 65Zn2+ from EDTA-treated cell walls were strictly temperature-dependent. The uptake of 22Na+ and 65Zn2+ in control and EDTA-treated cells appeared to be enzymatically controlled and could be inhibited by p-chloromercuribenzoate. The uptake and release of these radioisotopes in treated cells was two to five times that of untreated, control cells. The release of lipopolysaccharide could be correlated with a change in permeability of cells when they were treated with EDTA.

scholarly journals Use of actinomycin D for the specific quenching of fluorescence of deoxyribonucleic acid in cells stained with acridine aminoderivatives.

1976 ◽  
Vol 24 (11) ◽  
pp. 1169-1172 ◽  
Author(s):  
A V Zelenin ◽  
E A Kirianova ◽  
V A Kolesnikov ◽  
N G Stepanova
Keyword(s):  
Acridine Orange ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Actinomycin D ◽  
Quenching Of Fluorescence ◽  
Approximate Estimation ◽  
In Cells

Actinomycin D specifically quenches the fluorescence of acridine orange and quinacrine bound to deoxyribonucleic acid in cytologic preparations, but does not change the fluorescence of these fluorochromes bound to RNA. The following fluorescence-cytochemical applications of techniques based on these findings can be suggested: (a) distinction between deoxyribonucleic acid and ribonucleic acid; (b) detection of double-stranded virus ribonucleic acid; (c) approximate estimation of the lengths of A-T sequences in deoxyribonucleic acid molecules.

scholarly journals Deoxyribonucleic acid-dependent ribonucleic acid polymerases from normal and polyoma-transformed BHK-21/C13 cells

1975 ◽  
Vol 145 (3) ◽  
pp. 509-516 ◽  
Author(s):  
R J Cooper ◽  
H M Keir
Keyword(s):  
Ionic Strength ◽  
Rna Polymerase ◽  
Dna Content ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Actinomycin D ◽  
Activity Ratio ◽  
Cell Types ◽  
Dna Templates ◽  
Alpha Amanitin

DNA-dependent RNA polymerase (EC 2.7.7.6) ACTIVITIES FROM NORMAL BHK-21/C13 cells and from BHK-21/C13 cells transformed by polyoma virus (PYY cells) were solubilized and fractionated on columns of DEAE-Sephadex. Various properties of the A and B enzymes from the two types of cell were compared. 1. The yields of polymerase relative to the DNA content of the nuclear preparations are similar for both cell types. 2. The ionic-strength optima of polymerases A and B are 12.5 mM and 100mM with respect to (NH4)2SO4 for both cell types. 3. The Mn2+/Mg2+ activity ratio (measured at the respective optimum for each cation) for polymerase A from BHK-21/C13 cells was 1.48 and for the polymerase A from PYY cells was 0.55. The corresponding ratios for polymerase B were 10.11 for BHK-21/C13 cells and 22.75 for PYY cells. 4. Minor differences in the ability of the A polymerases to transcribe native and denatured DNA templates were observed; such differences were not apparent when the B polymerases were compared. 5. All the polymerases were inhibited completely by actinomycin D and by rifampicin AF/013, but not markedly so by rifampicin. Alpha-amanitin inhibited polymerase B but not polymerase A.

scholarly journals SOME BIOCHEMICAL PROPERTIES OF CHINESE HAMSTER CELLS SENSITIVE AND RESISTANT TO ACTINOMYCIN D

1974 ◽  
Vol 63 (3) ◽  
pp. 773-779 ◽  
Author(s):  
Robert H. F. Peterson ◽  
Judith A. O'Neil ◽  
June L. Biedler
Keyword(s):  
Rna Synthesis ◽  
Actinomycin D ◽  
Chinese Hamster ◽  
Biochemical Properties ◽  
Metabolic Inhibitors ◽  
Drug Resistant ◽  
Temperature Dependent ◽  
Chinese Hamster Cells ◽  
Uptake And Release ◽  
Resistant Cells

A graded series of drug-resistant Chinese hamster sublines has been examined for biochemical changes accompanying resistance to actinomycin D. The most highly resistant subline, DC-3F/AD X, is maintained at 10 µg/ml of the antibiotic. It was shown that over 250 times more actinomycin D is required to inhibit RNA synthesis in this subline than in the parental DC-3F line. The DC-3F/AD X subline was also shown to have a somewhat reduced capacity to transport uridine as compared to parental cells. Sensitive cells took up over 50 times more tritiated antibiotic than the most resistant cells, as determined in a 1-h assay. Uptake of actinomycin D was shown to be temperature-dependent in both resistant and sensitive cells and was not influenced by various metabolic inhibitors. Resistance could not be explained by a rapid uptake and release of the antibiotic, as demonstrated in efflux experiments, or by its metabolism. In addition, highly resistant cells which are cross-resistant to puromycin were shown to have a reduced capacity to take up labeled puromycin. These studies provide further evidence indicating that the mechanism of resistance to actinomycin D is reduced permeability to drug and suggesting that cell membrane alteration accounts for resistance to both actinomycin D and puromycin.

scholarly journals The Enzymatic Phosphorylation of Ribonucleic Acid and Deoxyribonucleic Acid

1966 ◽  
Vol 241 (12) ◽  
pp. 2933-2943 ◽  
Author(s):  
Abraham Novogrodsky ◽  
Moshe Tal ◽  
Abraham Traub ◽  
Jerard Hurwitz
Keyword(s):  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Enzymatic Phosphorylation
Sign in / Sign up

Export Citation Format

Share Document