Salt response of amylase produced in media of different NaCl or KCl concentrations by a moderately halophilic Micrococcus

1972 ◽  
Vol 18 (10) ◽  
pp. 1617-1620 ◽  
Author(s):  
Hiroshi Onishi
Keyword(s):  
Salt Concentration ◽  
Response Patterns ◽  
Moderately Halophilic ◽  
Amylase Production ◽  
Salt Response

Amylase production by a moderately halophilic Micrococcus sp. was influenced by the salt concentration of the medium, whereas salt-response patterns of amylase produced in media of 0.75 to 3.0 M NaCl or KCl were found to be almost identical.

Effects of salts on the aspartate transcarbamylase of a halophilic eubacterium, Vibrio costicola

1989 ◽  
Vol 67 (9) ◽  
pp. 666-669 ◽  
Author(s):  
Ijeoma Ahonkhai ◽  
Masahiro Kamekura ◽  
Donn J. Kushner
Keyword(s):  
Saccharomyces Cerevisiae ◽  
High Activity ◽  
Ammonium Sulphate ◽  
Prolonged Storage ◽  
Response Patterns ◽  
Aspartate Transcarbamylase ◽  
Moderately Halophilic ◽  
Low Salt ◽  
Salt Response ◽  
Atcase Activity

The aspartate transcarbamylase (ATCase) in cell-free extracts of the moderately halophilic eubacterium, Vibrio costicola, was stable in 1.5 M NaCl, but not in 0.5 M NaCl on prolonged storage at 4 °C in concentrated extracts. At lower salt concentrations, activity was lost rapidly. ATCase activity was optimal at about 1.5 M NaCl or 1.0 M KCl, although high activity was detected at 0.15 M NaCl. In the presence of 0.03 M aspartate both succinate and maleate inhibited ATCase activity. CTP inhibited the activity of the enzyme at low salt concentrations (0.15 to 0.3 M). Much less inhibition occurred at higher salt concentrations. Precipitating the enzyme with ammonium sulphate resulted in loss of CTP inhibition. The ATCase of V. costicola differs from those of a nonhalophile (Saccharomyces cerevisiae) and an extremely halophilic archaebacterium (Halobacterium cutirubrum) in its salt-response patterns of activity and regulation.Key words: halophilic, aspartate transcarbamylase, Vibrio costicola.

scholarly journals Draft Genome Sequence ofHalobacillussp. Strain KGW1, a Moderately Halophilic and Alkaline Protease-Producing Bacterium Isolated from the Rhizospheric Region ofPhragmites karkafrom Chilika Lake, Odisha, India

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Ananta Narayan Panda ◽  
Samir R. Mishra ◽  
Lopamudra Ray ◽  
Neha Sahu ◽  
Ankita Acharya ◽  
...  
Keyword(s):  
Water Sample ◽  
Salt Concentration ◽  
Toxic Metals ◽  
Genome Annotation ◽  
Alkaline Protease ◽  
Draft Genome ◽  
Gene Clusters ◽  
Bacterial Dna ◽  
Moderately Halophilic ◽  
Chilika Lake

Halobacillussp. strain KGW1 is a moderately halophilic, rod shaped, Gram-positive, yellow pigmented, alkaline protease-producing bacterium isolated from a water sample from Chilika Lake, Odisha, India. Sequencing of bacterial DNA assembled a 3.68-Mb draft genome. The genome annotation analysis showed various gene clusters for tolerance to stress, such as elevated pH, salt concentration, and toxic metals.

Influence of salt concentration on the susceptibility of moderately halophilic bacteria to antimicrobials and its potential use for genetic transfer studies

1995 ◽  
Vol 31 (6) ◽  
pp. 365-371 ◽  
Author(s):  
Mar�a-Jos� Coronado ◽  
Carmen Vargas ◽  
Hans J�rg Kunte ◽  
Erwin A. Galinski ◽  
Antonio Ventosa ◽  
...  
Keyword(s):  
Salt Concentration ◽  
Halophilic Bacteria ◽  
Genetic Transfer ◽  
Moderately Halophilic ◽  
Potential Use ◽  
Moderately Halophilic Bacteria

THE GLYCEROL DEHYDROGENASES OF PSEUDOMONAS SALINARIA, VIBRIO COSTICOLUS, AND ESCHERICHIA COLI IN RELATION TO BACTERIAL HALOPHILISM

1954 ◽  
Vol 32 (1) ◽  
pp. 206-217 ◽  
Author(s):  
R. M. Baxter ◽  
N. E. Gibbons
Keyword(s):  
Escherichia Coli ◽  
Sodium Chloride ◽  
Potassium Chloride ◽  
Salt Concentration ◽  
Halophilic Bacteria ◽  
Molar Concentration ◽  
Moderately Halophilic ◽  
E Coli ◽  
High Concentrations ◽  
Chloride Concentrations

Glycerol dehydrogenases from the extremely halophilic Pseudomonas salinaria and the moderately halophilic Vibrio costicolus are described and compared with the corresponding enzyme from the nonhalophilic Escherichia coli. The properties of all three enzymes are similar except their responses to salt concentration. The enzymes from E. coli and V. costicolus are most active at sodium chloride concentrations of about 0.25 M and 0.5 M respectively; that from P. salinaria is not only most active in the presence of 1.5 M NaCl but is irreversibly inactivated in the absence of salt. All three enzymes are more active in the presence of potassium chloride than of sodium chloride at any given molar concentration. These results suggest that the extremely halophilic bacteria contain high concentrations of salt and that their enzymes function maximally at these high concentrations. In contrast the moderately halophilic organisms contain relatively little salt and their enzymes are more comparable with those of nonhalophiles.

The Synthesis of Poly-β-Hydroxybutyrate by Moderately Halophilic Bacteria Halomonas venusta

2014 ◽  
Vol 1033-1034 ◽  
pp. 306-310 ◽  
Author(s):  
Shuang Gao ◽  
Ling Hua Zhang
Keyword(s):  
Salt Concentration ◽  
Halophilic Bacteria ◽  
Carbon Sources ◽  
Dry Weight ◽  
Phosphate Concentration ◽  
Two Phase ◽  
Moderately Halophilic ◽  
Initial Carbon ◽  
Carbon Nitrogen Ratio ◽  
Moderately Halophilic Bacteria

In this study, poly-β-hydroxybutyrate (PHB) was synthesized by moderately halophilic bacteria Halomonas venusta and identified by 1H-NMR. The effects of different carbon sources, salt concentration, initial carbon nitrogen quality ratio, initial phosphate concentration were studied on the synthesis of PHB. The optimum conditions of the PHB synthesis were detemined. Glucose was as carbon source with the concentration of 80 g/L and salt concentration, initial carbon nitrogen ratio, initial phosphate concentration was 3%, 15 and 1.9 g/L respectively. Under the above conditions, a two-phase synthesis system of PHB was constructed. The PHB synthesis amount and yield of cell dry weight was reached up to 1.5 g/L and 69.19 wt.%, respectively. The efficient synthesis of PHB was realized.

Nutrition and distribution of salt response in populations of moderately halophilic bacteria

1970 ◽  
Vol 16 (4) ◽  
pp. 253-261 ◽  
Author(s):  
M. P. Forsyth ◽  
D. J. Kushner
Keyword(s):  
Salt Concentration ◽  
Halophilic Bacteria ◽  
Nutrient Supply ◽  
Nutritional Requirements ◽  
Glucose Medium ◽  
Salt Tolerant ◽  
Replica Plating ◽  
Ph Values ◽  
Salt Response ◽  
Moderately Halophilic Bacteria

Cultures of Micrococcus halodenitrificans and Vibrio costicolus can grow in the presence of 0.4–3.5 M NaCl. Three lines of investigation: attempts to select for more or less salt-tolerant cells; colony counts at different salt concentrations; and replica plating experiments, suggested that populations of these organisms were genetically homogeneous in their salt response. That is, each cell in a population could grow over the whole range of salt concentration in which the culture grew.The nutritional requirements of V. costicolus were studied. This organism can grow in a minimal (salts–glucose) medium at pH values above 6.0 and in a salt concentration range 0.5–2.2 M. Increasing the concentration of phosphate buffer greatly increased cell yield. The range of salt concentration in which V. costicolus could grow depended on the nutrient supply, being widest in complex media. A wider range was possible in the presence of amino acids than in a salts–glucose medium. All growth factors permitting growth to occur at high salt concentrations were not identified.

THE GLYCEROL DEHYDROGENASES OF PSEUDOMONAS SALINARIA, VIBRIO COSTICOLUS, AND ESCHERICHIA COLI IN RELATION TO BACTERIAL HALOPHILISM

1954 ◽  
Vol 32 (3) ◽  
pp. 206-217 ◽  
Author(s):  
R. M. Baxter ◽  
N. E. Gibbons
Keyword(s):  
Escherichia Coli ◽  
Sodium Chloride ◽  
Potassium Chloride ◽  
Salt Concentration ◽  
Halophilic Bacteria ◽  
Molar Concentration ◽  
Moderately Halophilic ◽  
E Coli ◽  
High Concentrations ◽  
Chloride Concentrations

Glycerol dehydrogenases from the extremely halophilic Pseudomonas salinaria and the moderately halophilic Vibrio costicolus are described and compared with the corresponding enzyme from the nonhalophilic Escherichia coli. The properties of all three enzymes are similar except their responses to salt concentration. The enzymes from E. coli and V. costicolus are most active at sodium chloride concentrations of about 0.25 M and 0.5 M respectively; that from P. salinaria is not only most active in the presence of 1.5 M NaCl but is irreversibly inactivated in the absence of salt. All three enzymes are more active in the presence of potassium chloride than of sodium chloride at any given molar concentration. These results suggest that the extremely halophilic bacteria contain high concentrations of salt and that their enzymes function maximally at these high concentrations. In contrast the moderately halophilic organisms contain relatively little salt and their enzymes are more comparable with those of nonhalophiles.

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