Modification of pigmentation and cell-wall structure of Rhodotorula aurantiaca by culture in the presence of sulfur amino acids

1972 ◽  
Vol 18 (4) ◽  
pp. 423-427 ◽  
Author(s):  
K. A. Killick
Keyword(s):  
Amino Acids ◽  
Cell Wall ◽  
Cell Walls ◽  
Helix Pomatia ◽  
Wall Structure ◽  
Carotenoid Pigments ◽  
Sulfur Amino Acids ◽  
Rhodotorula Aurantiaca ◽  
Indirect Consequence ◽  
Increased Sensitivity

Growth of Rhodotorula aurantiaca in a medium supplemented with sulfur amino acids led to the synthesis and accumulation of S-adenosylmethionine, which was accompanied by a reduction in the total contents of carotenoid pigments and an increased sensitivity of the cell walls to snail gut enzymes (Helix pomatia) as judged by spheroplast formation. Walls isolated from supplemented cultures contained increased amounts of lipid and mannan but had a reduced content of protein and alkali-insoluble carbohydrate. The chemical modifications of the cell wall, which resulted as an indirect consequence of excessive S-adenosylmethionine synthesis, are interpreted to explain the ease with which sulfur amino acid-supplemented cultures of R. aurantiaca are converted to spheroplasts as well as their tendency to aggregate.

HYPHAL CELL WALL STRUCTURE OF TWO SPECIES OF PYTHIUM

1966 ◽  
Vol 12 (3) ◽  
pp. 471-475 ◽  
Author(s):  
Ralph Mitchell ◽  
Naama Sabar
Keyword(s):  
Amino Acids ◽  
Cell Wall ◽  
Cell Walls ◽  
Cell Wall Structure ◽  
Wall Structure ◽  
Pythium Myriotylum ◽  
Hyphal Cell

Hyphal cell walls were prepared by ultrasonication. Glucose was the only sugar detected in both species. Evidence was obtained indicating that the Pythium butleri Subram. glucan is beta 1,2-linked, and that the glucan of Pythium myriotylum Drechsler is beta 1,4-linked. Sixteen amino acids were detected in P. butleri cell wall hydrolysates. Four of these amino acids were absent from P. myriotylum preparations. Similar quantities of lipid were found in both species. The significance of these data for fungal classification is discussed.

scholarly journals Novel tool to quantify cell wall porosity relates wall structure to cell growth and drug uptake

2019 ◽  
Vol 218 (4) ◽  
pp. 1408-1421 ◽  
Author(s):  
Xiaohui Liu ◽  
Jiazhou Li ◽  
Heyu Zhao ◽  
Boyang Liu ◽  
Thomas Günther-Pomorski ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cell Walls ◽  
Dynamic Structure ◽  
Cell Types ◽  
Antifungal Drugs ◽  
Drug Uptake ◽  
Wall Structure ◽  
Quenching Efficiency ◽  
Model Plant Arabidopsis Thaliana ◽  
Cell Wall Porosity

Even though cell walls have essential functions for bacteria, fungi, and plants, tools to investigate their dynamic structure in living cells have been missing. Here, it is shown that changes in the intensity of the plasma membrane dye FM4-64 in response to extracellular quenchers depend on the nano-scale porosity of cell walls. The correlation of quenching efficiency and cell wall porosity is supported by tests on various cell types, application of differently sized quenchers, and comparison of results with confocal, electron, and atomic force microscopy images. The quenching assay was used to investigate how changes in cell wall porosity affect the capability for extension growth in the model plant Arabidopsis thaliana. Results suggest that increased porosity is not a precondition but a result of cell extension, thereby providing new insight on the mechanism plant organ growth. Furthermore, it was shown that higher cell wall porosity can facilitate the action of antifungal drugs in Saccharomyces cerevisiae, presumably by facilitating uptake.

scholarly journals Investigation of the Mechanical Properties of Flax Cell Walls during Plant Development: The Relation between Performance and Cell Wall Structure

Fibers ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 6 ◽  
Author(s):  
Camille Goudenhooft ◽  
David Siniscalco ◽  
Olivier Arnould ◽  
Alain Bourmaud ◽  
Olivier Sire ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cell Wall ◽  
Plant Development ◽  
Cell Walls ◽  
Cell Wall Structure ◽  
Wall Structure

scholarly journals The composition of the cell wall of Fusicoccum amygdali

1971 ◽  
Vol 125 (2) ◽  
pp. 461-471 ◽  
Author(s):  
K. W. Buck ◽  
M. A. Obaidah
Keyword(s):  
Cell Wall ◽  
Sodium Hydroxide ◽  
Cell Walls ◽  
Digestive Enzymes ◽  
Helix Pomatia ◽  
Water Soluble ◽  
Extracellular Polysaccharides ◽  
Lytic Enzymes ◽  
Similar Nature ◽  
Dark Blue

1. The cell wall of Fusicoccum amygdali consisted of polysaccharides (85%), protein (4–6%), lipid (5%) and phosphorus (0.1%). 2. The main carbohydrate constituent was d-glucose; smaller amounts of d-glucosamine, d-galactose, d-mannose, l-rhamnose, xylose and arabinose were also identified, and 16 common amino acids were detected. 3. Chitin, which accounted for most of the cell-wall glucosamine, was isolated in an undegraded form by an enzymic method. Chitosan was not detected, but traces of glucosamine were found in alkali-soluble and water-soluble fractions. 4. Cell walls were stained dark blue by iodine and were attacked by α-amylase, with liberation of glucose, maltose and maltotriose, indicating the existence of chains of α-(1→4)-linked glucopyranose residues. 5. Glucose and gentiobiose were liberated from cell walls by the action of an exo-β-(1→3)-glucanase, giving evidence for both β-(1→3)- and β-(1→6)-glucopyranose linkages. 6. Incubation of cell walls with Helix pomatia digestive enzymes released glucose, N-acetyl-d-glucosamine and a non-diffusible fraction, containing most of the cell-wall galactose, mannose and rhamnose. Part of this fraction was released by incubating cell walls with Pronase; acid hydrolysis yielded galactose 6-phosphate and small amounts of mannose 6-phosphate and glucose 6-phosphate as well as other materials. Extracellular polysaccharides of a similar nature were isolated and may be formed by the action of lytic enzymes on the cell wall. 7. About 30% of the cell wall was resistant to the action of the H. pomatia digestive enzymes; the resistant fraction was shown to be a predominantly α-(1→3)-glucan. 8. Fractionation of the cell-wall complex with 1m-sodium hydroxide gave three principal glucan fractions: fraction BB had [α]D +236° (in 1m-sodium hydroxide) and showed two components on sedimentation analysis; fraction AA2 had [α]D −71° (in 1m-sodium hydroxide) and contained predominantly β-linkages; fraction AA1 had [α]D +40° (in 1m-sodium hydroxide) and may contain both α- and β-linkages.

Die gemeinsame Wurzel der Lyse von Escherichia coli durch Penicillin oder durch Phagen

1957 ◽  
Vol 12 (7) ◽  
pp. 421-427 ◽  
Author(s):  
W. Weidel ◽  
J. Primosigh
Keyword(s):  
Cell Wall ◽  
Cell Walls ◽  
Building Blocks ◽  
Diaminopimelic Acid ◽  
Wall Structure ◽  
Gram Positive ◽  
E Coli ◽  
Growing Cell ◽  
Cell Wall Disruption ◽  
Entire Cell

One of the two layers of the E. coli B cell wall is shown to possess the chemical composition typical of a gram-positive microorganism. It is this layer which lends support and strength to the entire cell wall structure, its rigidity depending up on the incorporation of building blocks made up from alanine, glutamic acid, diaminopimelic acid, muramic acid and glucosamine.Phage enzyme is an agent capable of removing these stabilizing units from the „gram-positive “ layer, thereby causing it to collapse. Penicillin appears to prevent the biosynthetic incorporation of the same stabilizing units into growing cell walls, thus producing eventually the effect of cell wall disruption in a basically similar way.The rather manifold aspects of these findings are discussed at some length.

Subcellular localization of the Streptococcus mutans P1 protein C terminus

1999 ◽  
Vol 45 (6) ◽  
pp. 536-539 ◽  
Author(s):  
Mary K Homonylo-McGavin ◽  
Song F Lee ◽  
George H Bowden
Keyword(s):  
Amino Acids ◽  
Monoclonal Antibody ◽  
Cell Wall ◽  
Streptococcus Mutans ◽  
Polyclonal Antibody ◽  
Cell Walls ◽  
Protein C ◽  
Protein Localization ◽  
Subcellular Location ◽  
C Terminus

To determine the subcellular location of the Streptococcus mutans P1 protein C-terminal anchor, cell envelope fractionation experiments were conducted in combination with Western immunoblotting, using monoclonal antibody MAb 6-8C specific for an epitope that maps near the C terminus of P1 protein and also a polyclonal antibody preparation directed against the P1 C-terminal 144 amino acids (P1COOH). P1 protein was detected in cell walls but not the membrane purified from S. mutans cells by the monoclonal antibody. In contrast, P1 protein was not detected in the same cell wall preparation using the anti-P1COOH polyclonal antibody. However, proteins released from the cell walls by treatment with mutanolysin contained antigen that was recognized by the anti-P1COOH antibody, suggesting that the epitopes recognized by the antibody were masked by peptidoglycan in the cell wall preparations. When cell walls were treated with boiling trichloroacetic acid to solubilize cell-wall-associated carbohydrate, P1 antigen could not be detected in either the solubilized carbohydrate, or in the remaining peptidoglycan, regardless of whether polyclonal or monoclonal antibody was used. However, when the peptidoglycan was treated with mutanolysin, P1 antigen could be detected in the mutanolysin solubilized fraction by MAb 6-8C. Collectively, these data suggest that the C-terminal 144 amino acids of the P1 protein are embedded within the cell wall, and associated exclusively with the peptidoglycan. Furthermore, the ability of the anti-P1COOH antibody to recognize P1 antigen only after mutanolysin treatment of cell walls suggests these C-terminal 144 amino acids are tightly intercalated within the peptidoglycan strands.Key words: antigen P1, cell wall proteins, fusion proteins, peptidoglycan, protein localization.

scholarly journals Chilling Injury in Peaches: A Cytochemical and Ultrastructural Cell Wall Study

1992 ◽  
Vol 117 (1) ◽  
pp. 114-118 ◽  
Author(s):  
J.G. Luza ◽  
R. van Gorsel ◽  
V.S. Polito ◽  
A.A. Kader
Keyword(s):  
Cell Wall ◽  
Cell Walls ◽  
Prunus Persica ◽  
Periodic Acid ◽  
Chilling Injury ◽  
Middle Lamella ◽  
Wall Structure ◽  
Positive Staining ◽  
Intercellular Matrix ◽  
Parenchyma Cells

Fruits of mid- (`O'Henry'), late (`Airtime'), and extra-late-season (`Autumn Gem') peach [Prunus persica (L.) Batsch] cultivars were examined for changes in cell wall structure and cytochemistry that accompany the onset of mealiness and leatheriness of the mesocarp due to chilling injury. The peaches were stored at 10C for up to 18 days or at SC for up to 29 days. Plastic-embedded sections were stained by the Schiff's-periodic acid reaction, Calcofluor white MR2, and Coriphosphine to demonstrate total insoluble carbohydrates, ß-1,4 glucans, and pectins, respectively. Mealiness was characterized by separation of mesocarp parenchyma cells leading to increased intercellular spaces and accumulation of pectic substances in the intercellular matrix. Little structural change was apparent in the cellulosic component of the cell walls of these fruits. In leathery peaches, the mesocarp parenchyma cells collapsed, intercellular space continued to increase, and pectin-positive staining in the intercellular matrix increased greatly. In addition, the component of the cell walls that stained positively for ß-1,4 glucans became thickened relative to freshly harvested or mealy fruit. At the ultrastructural level, dissolution of the middle lamella, cell separation, irregular thickening of the primary wall, and plasmolysis of the mesocarp parenchyma cells were seen as internal breakdown progressed.

scholarly journals Aplikasi Kalsium dan NAA untuk Mengendalikan Getah Kuning Buah Manggis (Garcinia mangostana L.)

2018 ◽  
Vol 9 (1) ◽  
pp. 10-18
Author(s):  
Yulinda Tanari ◽  
Darda Efendi ◽  
Roedhy Poerwanto ◽  
Didy Sopandie ◽  
Ketty Suketi
Keyword(s):  
Cell Wall ◽  
Cell Division ◽  
Cell Walls ◽  
Block Design ◽  
Wall Structure ◽  
Sink Strength ◽  
Naphthaleneacetic Acid ◽  
Garcinia Mangostana ◽  
Random Block ◽  
Random Block Design

ABSTRACTThe yellow sap is produced naturally in mangosteen organ except in the root. The yellow sap contaminated the aryl and rind if the epithelial cell walls rupture due to deficiency of calcium (Ca). Calcium is one of structural component of cell walls, whereas naphthaleneacetic acid (NAA) has its role in improving cell division and cell elongation. Interaction of Ca and NAA can improve sink strength and capacity because the newly formed cells need Ca to construct wall structure. This experiment aimed at finding out the effect of Ca and NAA applications in reducing the contamination of yellow sap in mangosteen. The experiment was conducted by using factorial random block design consisting of 2 factors and 3 replications. The first factor was Ca dosage (0 and 4.8 kg/tree), and the second factor was NAA concentration (0, 200, 400 and 600 ppm). The results showed that application of 4.8 Ca/tree and 200 ppm NAA as much as 5 ml / fruit effectively improve the content of Ca pectate in pericarp, reduced the percentage of yellow sap contamination on the fruit segment, aryl and rind to 0% and 12.3% respectively compared to control (17.8% on fruit segment, 36.8% on aryl and 56.1% on rind).Key words: aryl, Ca pectate, cell wall, middle lamela.ABSTRAKGetah kuning adalah getah yang dihasilkan secara alami pada setiap organ manggis, kecuali pada akar. Getah kuning akan keluar dan mencemari aril serta kulit jika dinding sel epitel pecah karena kekurangan kalsium (Ca). Kalsium adalah komponen dinding sel, berperan dalam struktur dan permeabilitas membran sedangkan asam naftalenasetat (NAA) berperan penting dalam meningkatkan pembelahan dan pembesaran sel. Interaksi keduanya dapat meningkatkan kapasitas sink buah karena sel yang baru terbentuk membutuhkan Ca dalam menyusun struktur dinding sel. Percobaan bertujuan untuk mengetahui pengaruh aplikasi Ca dan NAA dalam menurunkan cemaran getah kuning manggis. Percobaan menggunakan rancangan acak kelompok faktorial 2 faktor dengan 3 ulangan. Faktor ke-1 yaitu dosis Ca (0 dan 4.8 kg Ca/pohon) dan faktor ke-2 yaitu konsentrasi NAA (0, 200, 400 dan 600 ppm) dengan volume semprot 5 ml perbuah. Hasil percobaan menunjukkan bahwa aplikasi 4.8 kg Ca/pohon dengan NAA 200 ppm sebanyak 5 ml/buah efektif meningkatkan kandungan Ca pektat perikarp dan menurunkan persentase buah tercemar getah kuning menjadi 0% pada juring dan aril serta 12.3% pada kulit dibandingkan dengan perlakuan kontrol (17.8% pada juring, 36.8% pada aril dan 56.1% pada kulit buah).Kata kunci: aril, Ca pektat, dinding sel, lamela tengah

scholarly journals Staphylococcus aureus cell wall structure and dynamics during host-pathogen interaction

2021 ◽  
Vol 17 (3) ◽  
pp. e1009468
Author(s):  
Joshua A. F. Sutton ◽  
Oliver T. Carnell ◽  
Lucia Lafage ◽  
Joe Gray ◽  
Jacob Biboy ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Cell Walls ◽  
Ex Vivo ◽  
Cell Wall Structure ◽  
Wall Structure ◽  
Bacterial Cells ◽  
Human Macrophages ◽  
Structure And Dynamics

Peptidoglycan is the major structural component of the Staphylococcus aureus cell wall, in which it maintains cellular integrity, is the interface with the host, and its synthesis is targeted by some of the most crucial antibiotics developed. Despite this importance, and the wealth of data from in vitro studies, we do not understand the structure and dynamics of peptidoglycan during infection. In this study we have developed methods to harvest bacteria from an active infection in order to purify cell walls for biochemical analysis ex vivo. Isolated ex vivo bacterial cells are smaller than those actively growing in vitro, with thickened cell walls and reduced peptidoglycan crosslinking, similar to that of stationary phase cells. These features suggested a role for specific peptidoglycan homeostatic mechanisms in disease. As S. aureus penicillin binding protein 4 (PBP4) has reduced peptidoglycan crosslinking in vitro its role during infection was established. Loss of PBP4 resulted in an increased recovery of S. aureus from the livers of infected mice, which correlated with enhanced fitness within murine and human macrophages. Thicker cell walls correlate with reduced activity of peptidoglycan hydrolases. S. aureus has a family of 4 putative glucosaminidases, that are collectively crucial for growth. Loss of the major enzyme SagB, led to attenuation during murine infection and reduced survival in human macrophages. However, loss of the other three enzymes Atl, SagA and ScaH resulted in clustering dependent attenuation, in a zebrafish embryo, but not a murine, model of infection. A combination of pbp4 and sagB deficiencies resulted in a restoration of parental virulence. Our results, demonstrate the importance of appropriate cell wall structure and dynamics during pathogenesis, providing new insight to the mechanisms of disease.

scholarly journals Bundling of cellulose microfibrils in native and polyethylene glycol-containing wood cell walls revealed by small-angle neutron scattering

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Paavo A. Penttilä ◽  
Michael Altgen ◽  
Muhammad Awais ◽  
Monika Österberg ◽  
Lauri Rautkari ◽  
...  
Keyword(s):  
Cell Wall ◽  
Neutron Scattering ◽  
Plant Cell ◽  
Small Angle ◽  
Cell Walls ◽  
Small Angle Neutron Scattering ◽  
Plant Cell Wall ◽  
Raw Materials ◽  
Cellulose Microfibrils ◽  
Wall Structure

AbstractWood and other plant-based resources provide abundant, renewable raw materials for a variety of applications. Nevertheless, their utilization would greatly benefit from more efficient and accurate methods to characterize the detailed nanoscale architecture of plant cell walls. Non-invasive techniques such as neutron and X-ray scattering hold a promise for elucidating the hierarchical cell wall structure and any changes in its morphology, but their use is hindered by challenges in interpreting the experimental data. We used small-angle neutron scattering in combination with contrast variation by poly(ethylene glycol) (PEG) to identify the scattering contribution from cellulose microfibril bundles in native wood cell walls. Using this method, mean diameters for the microfibril bundles from 12 to 19 nm were determined, without the necessity of cutting, drying or freezing the cell wall. The packing distance of the individual microfibrils inside the bundles can be obtained from the same data. This finding opens up possibilities for further utilization of small-angle scattering in characterizing the plant cell wall nanostructure and its response to chemical, physical and biological modifications or even in situ treatments. Moreover, our results give new insights into the interaction between PEG and the wood nanostructure, which may be helpful for preservation of archaeological woods.

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