The genetic control of the two types of mannan produced by Saccharomyces cerevisiae

1971 ◽  
Vol 17 (11) ◽  
pp. 1451-1454 ◽  
Author(s):  
J. F. T. Spencer ◽  
P. A. J. Gorin ◽  
G. H. Rank
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Magnetic Resonance ◽  
Genetic Control ◽  
Spectral Type ◽  
Proton Magnetic Resonance ◽  
Side Chains ◽  
Type B ◽  
Complex Type ◽  
Mendelian Gene ◽  
Different Strains

The two mannans formed by different strains of Saccharomyces cerevisiae give proton magnetic resonance spectra (spectral types A and B), which suggests that they differ principally in the presence of a-(1 → 3)-linked mannopyranosyl end units of the side chains of the more complex type (giving spectral type B). The presence or absence of such end units was under the control of a single mendelian gene, since the two types of mannan segregated independently during sporulation. The gene controlling this type of mannan produced was apparently not linked to those controlling the requirements for adenine, uracil, leucine, or histidine. The implications of the findings concerning the mechanism of biosynthesis of yeast mannan are discussed.

Proton Magnetic Resonance of Proteins Fully Deuterated except for 1H-Leucine Side Chains

Science ◽  
1968 ◽  
Vol 161 (3843) ◽  
pp. 795-796 ◽  
Author(s):  
H. L. Crespi ◽  
R. M. Rosenberg ◽  
J. J. Katz
Keyword(s):  
Magnetic Resonance ◽  
Proton Magnetic Resonance ◽  
Side Chains

Structures of yeast mnnans containing both α- and β-linked D-mannopyranose units

1969 ◽  
Vol 47 (9) ◽  
pp. 1499-1505 ◽  
Author(s):  
P. A. J. Gorin ◽  
J. F. T. Spencer ◽  
S. S. Bhattacharjee
Keyword(s):  
Magnetic Resonance ◽  
Proton Magnetic Resonance ◽  
Main Chain ◽  
Specific Rotation ◽  
Substantial Proportion ◽  
Side Chains ◽  
Structural Investigations ◽  
Magnetic Resonance Spectra

Several mannans from yeasts give proton magnetic resonance spectra with distinctive H-1 signals at higher field than τ 4.55. A number of these mannans were investigated polarimetrically and each has a specific rotation corresponding to a mixture of α- and β-D-linkages. Four were partially hydrolyzed and shown to have most of the α-linkages in the main chains and the β-linkages in the side chains. Detailed structural investigations showed that the Pichiapastoris mannan probably has an α-(1→6)-D-manno-pyranosyl main-chain substituted in the 2-positions with a substantial proportion of O-α-D-mannopy-ranosyl-(1→2)-O-β-D-mannopyranosyl-(1→2)-O-β-D-mannopyranosyl-(1→2)-O-α-mannopyranosyl side-chains (1). The related mannan from Citeromycesmatritensis has a similar main-chain, which is substituted in the 2-positions by α-D-mannopyranosyl units and β-(1→2)-pyranose-linked D-mannotriosyl and perhaps mannobiosyl side-chains (2).

Comparison of proton magnetic resonance spectra of cell-wall mannans and galactomannans of selected yeasts with their chemical structures

1969 ◽  
Vol 47 (19) ◽  
pp. 3569-3576 ◽  
Author(s):  
P. A. J. Gorin ◽  
J. F. T. Spencer ◽  
R. J. Magus
Keyword(s):  
Cell Wall ◽  
Magnetic Resonance ◽  
Proton Magnetic Resonance ◽  
Side Chains ◽  
Chemical Structures ◽  
Degree Of Similarity ◽  
Magnetic Resonance Spectra

Yeasts can often be identified with the aid of the distinctive proton magnetic resonance (p.m.r.) spectra of their cell-wall mannans or galactomannans. Some of the polysaccharides, such as the galactomannan of Torulopsisgropengiesseri and the mannan of Saccharomycesrouxii give p.m.r. spectra with only slight differences and have closely related chemical structures in terms of main chains and side chains. Other spectrally similar polysaccharides with related chemical structures have also been found. These are firstly the galactomannans of Trichosporonfermentans and Candidalipolytica and secondly the mannose-containing polysaccharides of Torulopsisbombi (Torulopsis species PRL 319-67), Torulopsis magnoliae and Torulopsisapicola (Hajsig strain). The mannans of Candidacatenulata and Endomycopsisfibuliger give p.m.r. spectra with a lesser degree of similarity, but have markedly different chemical structures. These results show that polysaccharides with similar p.m.r. spectra have related chemical structures and may therefore arise from phylogenetically related yeasts.

Galactomannans of Trichosporon fermentans and other yeasts; proton magnetic resonance and chemical studies

1968 ◽  
Vol 46 (13) ◽  
pp. 2299-2304 ◽  
Author(s):  
P. A. J. Gorin ◽  
J. F. T. Spencer
Keyword(s):  
Magnetic Resonance ◽  
Proton Magnetic Resonance ◽  
Main Chain ◽  
Resonance Spectrum ◽  
Proton Magnetic Resonance Spectrum ◽  
Yeast Cells ◽  
Side Chains ◽  
Trichosporon Fermentans ◽  
Chemical Studies ◽  
Magnetic Resonance Spectra

A survey of the alkali soluble polysaccharides of yeast cells shows that galactomannans are formed by certain species of the genera Trichosporon, Schizosaccharomyces, and Nadsonia. Different species of Trichosporon form galactomannans, pentosylmannans, and a mannan. The galactomannans are distinguishable from those of Schizosaccharomyces by the H-1 region of their proton magnetic resonance spectra (recorded at 70° in D2O using an HA-100 Varian spectrometer), but the spectra of Trichosporon hellenicum and Nadsonia fulvescens galactomannans are virtually indistinguishable.The proton magnetic resonance spectrum of the Trichosporon fermentans galactomannan contains fewer signals than those of other Trichosporon galactomannans. The polymer consists predominantly of a main-chain containing a high proportion of (1 → 6)-mannopyranosyl units substituted in their 2-positions by O-α-D-galactopyranosyl-(1 → 2)-O-α-D-mannopyranosyl side-chains (1).

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